47 results about "Exopalaemon carinicauda" patented technology

The invention relates to an environment-friendly feed for an exopalaemon carinicauda. The feed comprises fish meal, fermented bean pulp, peanut meal, arginine, glycine, wheat gluten, wheat, high protein flour, defatted rice bran, brewers' yeast, shrimp shell powder, clam ferment powder, fish oil, vegetable oil, unshell elements, compound enzyme preparations, compound vitamins, compound organic trace elements, choline chloride and monocalcium phosphate for feed. In addition, feed ingredient further comprises 500-800U/kg of phytase. Due to adoption of the environment-friendly feed for the exopalaemon carinicauda, so that a nutritional demand of an optimal growth of the exopalaemon carinicauda can be met, a feed utilization ratio is improved, and pollution to a water environment during a breeding process of the exopalaemon carinicauda is reduced. Meanwhile, peptides are applied to improve immunity of the exopalaemon carinicauda, and a sustainable development of a seawater breeding industry of the exopalaemon carinicauda can be realized.

A method for improving the current-year small-specification fingerling overwintering survival rate of fugu obscurus is composed of four production steps which are small-specification fingerling concentrated breeding, temporary culture adaptation, forced domestication and reinforced cultivation. The method is characterized by comprising the steps of placing small-specification thin fingerlings which are yellow in body color in the current year in an indoor cement pool in a concentrated mode ahead of time in the last ten days of October prior to grinding wheel when the water temperature is 20 DEG C, feeding fresh and live opossum shrimp or exopalaemon carinicauda during an adaptation period of 2-3 days for the small-specification fingerlings just subjected to concentrated breeding, starting forced domestication with matched feed after stopping feeding the fresh and live baits for 2-3 days, continuing the domestication for 7-10 days, feeding twice per day during the reinforced cultivation period, sucking the bottom to discharge dirt per day with the feeding for 2 hours as the standard, keeping water quality fresh, adopting circulating water or replacing the water twice per week, replacing 2/3 of water each time, maintaining the water temperature at over 15 DEG C, and adopting a fugu obscurus conventional method to overwinter after continuing the reinforced cultivation for 18-22 days.

The invention relates to an artificial maturing method for exopalaemon carinicauda in anestrous seasons, in particular to an artificial reproduction method for exopalaemon carinicauda in autumns and winters by means of regulating and controlling the temperature and the illumination cycle and enriching nutrients for the exopalaemon carinicauda. The artificial maturing method mainly includes controlling the temperature of water; regulating and controlling the illumination cycle; and enriching nutrients for the exopalaemon carinicauda. Temperature control equipment simulates a procedure that the temperature of water in springs rises gradually so as to control the temperature of the water; fluorescent lamps and a timer simulate a procedure that the illumination cycle is gradually prolonged in the springs so as to regulate and control the illumination cycle; and fresh baits such as nereid are fed to the exopalaemon carinicauda to enrich nutrient supplied for the exopalaemon carinicauda in a gonad development procedure. The exopalaemon carinicauda can smoothly reproduce by laying eggs after being bred for 2-3 weeks under the condition. The artificial maturing method has the advantages that characteristics that the temperature of the water gradually rises in the springs and the illumination cycle is gradually prolonged under natural conditions are simulated, so that gonads of the exopalaemon carinicauda are developed in the anestrous seasons, the exopalaemon carinicauda reproduces by laying the eggs in the anestrous seasons, and the purpose of controlling reproduction of the exopalaemon carinicauda throughout the year is achieved.

The invention relates to a method for obviously improving the survival rate of exopalaemon carinicauda in the artificial breeding process, in particular to a method for carrying out larva separation and collection by utilizing phototaxis of larvae. The method comprises the following steps of dividing different functional regions in a spawning pool; regulating illumination in different functional regions; and installing a lifting adjustable larva collection net. In the step of dividing the functional regions, a large-aperture screen is utilized to isolate and divide the spawning pool into a parent shrimp action region and a larva collection region; and illumination regulation comprises the steps of covering the parent shrimp action region with a sunshade net and carrying out lure in the larva collection region by a fluorescent lamp. The method has the advantages that division of different functional regions and illumination regulation enable larvae and parent shrimps to be spatially and effectively separated and reduces the predation rate of the parent shrimps for the larvae, so that post-larvae yield is greatly improved; meanwhile, embryo abscission and death of the parent shrimps, which are caused by frequent sorting of the parent shrimps, are also avoided and the survival rate in the breeding process is improved; and the lifting adjustable larva collection net can be used for collecting larvae in due time according to the hatching condition of larvae, so that labor intensity is reduced and the practical value on production is improved.

The technology belongs to the technical field of aquiculture disease prevention, in particular to the preparation method of the immune reinforcing agent for cultivating crustacean, and the applicationmethod thereof. The aquiculture composite immune reinforcing agent comprises 2-4 parts of aquiculture extractive or baicalin and 2.5-5 parts of levamisole. The composite immune reinforcing agent caneffectively enhance non-specific immunity for cultivating blue crab, improve the activity of the blood serum phenol oxidase, the superoxide dismutase and the alkalinephosphatase of the blue crab, andimproves the disease resistance of the blue crab to common pathogens such as white spot syndrome virus, vibrio, hematodinium, etc. The composite immune reinforcing agent can be applied to the cultivation of the blue crab by being injected or added into blue crab fresh fish bait and granulated feed for preventing and controlling the disease at the disease peak and reducing the disease rate and themortality rate of the blue crab. The immune reinforcing agent is added into the feed for preventing and controlling the disease of the penaeus vannamei boone and exopalaemon carinicauda.

The invention discloses a method for synchronous line establishment of small-population parents in artificial breeding of exopalaemon carinicauda. In non-high-breeding season at relatively low water temperature, water temperature is regulated and controlled in three development stages (parent prawn gonad development stage, zygote embryo development stage and larval development stage) by virtue of a temperature control method, so that families established by small-population parents in different gonad development stages can develop into a post-larval stage at the same time as much as possible, and the synchronous line establishment of the small-population parents is finally achieved. By respectively setting temperature gradients according to the suitable temperature ranges of the exopalaemon carinicauda in different development stages, the method disclosed by the invention is conducive to the shortening of development time difference of various parent progenies, and capable of achieving the synchronous line establishment and multi-generation breeding per year of the small-population parents in the artificial breeding of the exopalaemon carinicauda.

The invention provides a method for detecting an EcSSR1024 microsatellite DNA marker in exopalaemon carinicauda, which comprises: extracting genomic DNA of the exopalaemon carinicauda and diluting the genomic DNA for later use; designing specific primers at the two ends of the core sequence of an EcSSR1024 microsatellite; performing the polymerase chain reaction (PCR) amplification of the genomicDNA of the exopalaemon carinicauda by using the primers, and detecting the product of the PCR amplification; and analyzing by using strips appeared in the product, and obtaining the polymorphic map of a highly-genetic variation in the core sequence area of the EcSSR1024 in the exopalaemon carinicauda. By using the primers and detection method, which are provided by the invention, the genetic variation in the core area of the EcSSR1024 microsatellite in each individual of exopalaemon carinicauda can be detected, and the polymorphic map of the highly-genetic variation in a genetic marker gene locus in the EcSSR1024 in exopalaemon carinicaud can be obtained quickly. The method is suitable to be used in population genetic marking, genealogical identification, genetic map construction and the like.

The invention discloses a method for rapidly breeding Cynoglossus Semilaevis Gunther in a soil pond, comprising the following steps of soil pond selecting, advanced fry stocking, breeding management, harvesting and the like. The invention has convenient and reasonable operation, and the survival rate reaches more than 90 percent. The invention can also be used for breeding aquatic products of Exopalaemon carinicauda and the like.

The invention provides a triple-PCR (Polymerase Chain Reaction) detection method for exopalaemon carinicauda by using a microsatellite mark. The triple-PCR detection method comprises the following steps of: designing and synthesizing a triple-PCR primer; extracting genome DNA (Deoxyribose Nucleic Acid); carrying out triple-PCR; and detecting a PCR product, wherein the PCR comprises a triple-PCR system and a triple-PCR program. According to the triple-PCR detection method disclosed by the invention, the triple-PCR system for identifying the family and the paternity of the exopalaemon carinicauda is established and synchronous detection of three microsatellite sites in one PCR is realized, so that the efficiency of the triple-PCR detection method is increased by three times compared with that of an original PCR method. The triple-PCR detection method disclosed by the invention has the characteristics of high efficiency, economy, simplicity, convenience, feasibility and the like and can be popularized and applied in genetic diversity analysis, genetic relationship identification, family management and selective breeding.

The invention provides a portunus trituberculatus and exopalaemon carinicauda polyculture feed and belongs to the technical field of fishery industry. The portunus trituberculatus and exopalaemon carinicauda polyculture feed is prepared from the following substances in parts by weight: 20-30 parts of red fishmeal, 5-10 parts of arginine, 8-15 parts of spiral seaweed powder, 4-6 parts of immunopolysaccharide, 10-15 parts of fermented soybean meal, 2-3 parts of pinus massoniana needle powder, 1-5 parts of vinasse, 2-6 parts of bacillus subtilis powder, 2-3 parts of fish oil, 10-15 parts of rice bran, 5-10 parts of nepeta cataria powder, 5-8 parts of cellulase, 0.2-0.5 part of compound vitamin, 0.5-1 part of compound mineral substance and 5-8 parts of houttuynia cordata powder. The portunus trituberculatus and exopalaemon carinicauda polyculture feed has balanced nutrition and can meet the daily requirements of portunus trituberculatus and exopalaemon carinicauda; the feed efficiency is high, the survival rate of the portunus trituberculatus and the exopalaemon carinicauda is high and the weight growth speed is high; after all the raw materials are reasonably compounded, the palatability and the food calling effect are good; the immunity of the portunus trituberculatus can be remarkably enhanced and the antibacterial and anti-virus capabilities of the portunus trituberculatus are improved; and the portunus trituberculatus and exopalaemon carinicauda polyculture feed has good stability in water and is environment-friendly.

The invention provides a method for constructing an Exopalaemon carinicauda reciprocal backcross family. The method includes the following steps: (1) parent shrimp selection; (2) parent shrimp mating; (3) cultivation and breeding of F1 offspring seeds; (4) parent-child backcross; and (5) cultivation and breeding of backcross progeny offspring seeds. Male and female Exopalaemon carinicauda parent shrimps which are distributed naturally or bred artificially and have excellent characters are selected, and the reciprocal backcross family can be acquired through completely-artificial breeding, aquaculture and parent-child mating; the success rate of construction of the reciprocal backcross family can reach 75%, and can provide material and technique support for quantitative character genetic analysis, near-isogenic line construction and back cross breeding of the Exopalaemon carinicauda.

An exopalaemon carinicauda feed is prepared by the following raw materials in parts by weight: 2-4 parts of clover powder, 0.2-0.4 part of soya lecithin, 0.3-0.5 part of peanut oil, 10-12 parts of rape cake, 8-12 parts of cottonseed cake, 2-4 parts of Chinese medical stone powder, 1-2 parts of zeolite powder, 3-5 parts of spirulina powder, 2-4 parts of silkworm chrysalis powder, 1-2 parts of earthworm powder, 2-5 parts of chitosan, 3-5 parts of peanut shell, 0.1-0.2 part of radix astragali, 0.3-0.5 part of isatis root, 0.1-0.2 part of finelydivided phtheirospermum root, 1-2 parts of salvia officinalis, 1-2 parts of Chinese angelica, 2-4 parts of chicken liver, 1-2 parts of chicken skin, 1-2 parts of rose root powder, 3-5 parts of potato, 20-25 parts of corn flour, 12-14 parts of wheat flour, and 1-2 parts of EM original liquid.

The invention relates to a method for selecting hypoxia-tolerant Exopalaemon carinicauda. The method includes: selecting live adult Exopalaemon carinicauda; preparing water with the dissolved oxygen content being not lower than 4ppm, and placing the adult Exopalaemon carinicauda into the water, wherein the water is isolated from atmosphere; when dead Exopalaemon carinicauda appears in the water along with dissolved oxygen content lowering, removing the dead Exopalaemon carinicauda from the water to prevent the water from being polluted by the decomposed dead Exopalaemon carinicauda; when the number of the live adult Exopalaemon carinicauda lowers to 10% of the initial adding quantity of the adult Exopalaemon carinicauda, collecting the live adult Exopalaemon carinicauda in the water. By the method, the adult Exopalaemon carinicauda individuals good in hypoxia tolerance can be screened out to facilitate follow-up breeding.

The invention provides an exopalaemon carinicauda livetin binding protein gene and application thereof. The sequence of the gene is shown in SEQ ID NO.1 in the description. The amino acid sequence encoded by the gene is shown in SEQ ID NO.2 in the description. An exopalaemon carinicauda livetin binding protein comprises three structure domains, namely EcVBP-PC1, EcVBP-PC2 and EcVBP-P3 respectively. The invention discloses application of the gene EcVBP in mediating a foreign protein into a zygote of a shelled animal through the livetin binding protein. By adopting the gene provided by the invention, the foreign protein is successfully mediated into a zygote of neocaridina denticulate sinensis, so that feasibility that VBP as a "molecule truck" carries the foreign protein into the zygote ofthe shelled animal is verified, and a basis is made for gene editing and molecular designing breeding of the shelled animal later.

The invention relates to an exopalaemon carinicauda compound eye development regulation gene EcEy which can generate obvious phenotypic change in an embryo period after being successfully edited so asto indicate the success rate of gene editing operation, and the gene can also be applied to the establishment of gene editing platforms of other decapod crustaceans. The EcEy gene of the exopalaemoncarinicauda has a nucleotide sequence shown as SEQ ID No. 1 in a sequence list; the guide RNA (gRNA) designed and synthesized on the basis of the sequence has a ribonucleic acid sequence shown as SEQID No.2 in a sequence list; EcEy-gRNA and commercial Cas 9 mRNA are mixed and injected into fertilized eggs of exopalaemon carinicauda, a PAX structural domain of an EcEy gene can be specifically recognized and cut, and an obvious compound eye defect phenotype is observed in the early stage of embryonic development. The EcEy gene can be used as a target gene for gene editing research of exopalaemon carinicauda, is used for verifying whether gene editing operation succeeds or not, and can also be used for establishing gene editing platforms of other decades of crustaceans.

The invention discloses a quantitative test method of exopalaemon carinicauda mitochondrial genome copy number. The method comprises the steps of selecting the adenylyl transferase gene (ANT) and the ATP6 respectively as the single copy genes of the nuclear genome and the mitochondrial genome of white tailed shrimp, designing the primer and the fluorescent probe suitable for the real time quantitative PCR reaction of the ANT and ATP6 genes, preparing real time quantitative PCR reaction standard of the ANT and ATP6 genes, through drawing the standard curve (as shown in the attachment), and using the standard curve as the standard, and quantifying and calculating the copy number of the mitochondrial genome in the to-be-tested sample cells. The method can correctly and quantitatively test the mitochondrial genome copy number of the exopalaemon carinicauda tissue sample cells, and has the advantages of being high in specificity, and simple in operation. Merely the fluorescence quantitative PCR instrument is enough to complete the quantification of the exopalaemon carinicauda mitochondrial genome copy number.

The invention discloses a method for acquiring ecological early breeding seeds of acanthogobius ommaturus Richardson, and relates to technologies for acquiring early seeds for early breeding for acanthogobius ommaturus Richardson. The method includes selecting parent fish; controlling the breeding quantities; selecting heat-insulation sheds of ponds; cultivating the parent fish through the winters; collecting fish fries. The method is characterized in that the parent fish with the weight ranging from 100 grams to 250 grams is selected in periods from November to December, and is cultivated in the ponds for takifugu rubripes through the winters, nylon films are constructed to form heat-insulation greenhouses on the upper sides of the ponds, the areas of the ponds preferably range from 3mu to 8mu, the ratio of female parent fish to male parent fish is 1:1, 10-12 pieces of parent fish is bred in each mu of the ponds under the control, 3-5 kilograms of live exopalaemon carinicauda are placed in each mu in winter cultivation periods and are used as fodders for the parent fish, water is changed regularly, an excellent water quality condition needs to be kept, 1.2-meter water levels of the ponds are kept, and the salinity of the water in the ponds keeps ranging from 6%o to 15%o; the fish fries are sampled after 20th of March in the next year, growth conditions of the fish fries are inspected, and the fish fries are timely collected and distributed when the sizes of the growing fish fries range from 4 centimeters to 6 centimeters.

The invention relates to an LAMP detection method for detecting the health state of exopalaemon carinicauda before and after white spot syndrome virus infection. According to the LAMP detection method, in allusion to a cDNA sequence of a gene of which the expression quantity is significantly increased in the early stage of WSSV outburst and which is obtained through massive screening in the exopalaemon carinicauda, 6 primers are designed, and a (Loop-mediated isothermal amplification, LAMP) detection method is established. By the LAMP detection method, nucleic acid extraction and purification are not required, the cost is low, the operation is convenient, and the detection process is fast and sensitive. By the LAMP detection method, diagnosing and prewarning effects can be quickly and effectively achieved in the early stage of the disease outburst of the WSSV-infected exopalaemon carinicauda.

The invention relates to a shrimp pond net cage combination breeding method. The method comprises the steps of shrimp pond modification, putting of a net cage, putting of exopalaemon carinicauda berried parent shrimps, putting of young penaeus monodon, putting of young inkfish, putting of stichopus japonicus fry, staged feeding of bait and the like. By means of the protection function of the net cage, exopalaemon carinicauda, penaeus monodon, inkfish and stichopus japonicus are introduced into the same breeding pond, the stability of an ecological system is improved, and the occurrence rate ofdiseases of exopalaemon carinicauda and penaeus monodon is effectively controlled. Meanwhile, a water body is fully utilized, the productivity of the water area is improved, the economic benefit is increased, and the method is suitable for being popularized in northern outdoor shrimp ponds in China.

The invention relates to a pond polyculture high-yield method for red-shell meretrix meretrix and exopalaemon carinicauda holthuis. The method comprises the following steps: a deep water tank and a peripheral platform are dug in the middle of a pond, and water is normally stored in the pond; after disinfection, water quality maintenance is carried out, and initial water fertilization is carried out; in March, seeding of the red-shell meretrix meretrix is completed; fertilizing water mainly in early May, and putting parent exopalaemon carinicauda holthuis from late May to early June; and before the first batch of shrimp seeds grow to 2.5 cm in the current year, the aquatic water body is stably yellow green or yellowish brown mainly by adjusting the algal facies of the water body, after the shrimp seeds grow to 2.5 cm to 3 cm, pellet feed is fed, and feeding is enhanced in September to October. According to the method, by setting the pond stocking sequence and time of the meretrix meretrix and the exopalaemon carinicauda holthuis, the win-win situation of breeding of the meretrix meretrix and the exopalaemon carinicauda holthuis is achieved through ecological niche complementation, close matching of varieties and breeding management operation of key nodes, and the purpose of high yield is achieved.