107 results about "Every Two Weeks" patented technology

The invention discloses a method for breeding a small water turtle. The method comprises the first step of selecting a high-quality small water turtle for incubation, the second step of carrying out watering 2-4 times every day during the incubation, the third step of embathing the incubated young turtle through normal saline, the fourth step of enabling the breeding area of the small water turtle to comprise a breeding pond, a sandbeach area and a grassland area, the fifth step of moving the young turtle to the breeding pond, the sixth step of feeding the young turtle with fodder twice every day when the water temperature of the breeding pond is 18-32 DEG C, the seventh step of changing water once every 3-6 days when the water temperature of the breeding pond is 18-32 DEG C, the eighty step of embathing the small water turtle in the whole pond with saline solutions every two weeks in the growing period of the small water turtle, the ninth step of additionally feeding the small water turtle with pill fodder twice every day in the spawning period of the small water turtle and the tenth step of enabling the small water turtle to start to be in hibernation when air temperature reduces to below 12 DEG C. Through the mode, according to the method for breeding the small water turtle, the growth cycle of the small water turtle is properly prolonged, and the artificial taming and breeding of the small water turtle is achieved.

The invention belongs to the technical field of cell engineering, and specifically relates to a method for obtaining Lolium L. embryogenic callus and maintaining regeneration capacity of the callus. According to the present invention, the material for callus induction is the mature seed embryos of the Lolium L.; the mature embryos are adopted to induce the callus, after the mature embryos are cultured for 1 month, 4 secondary cultures are performed, wherein the time of the 4 secondary cultures is 2 months, and the secondary culture is performed every 2 weeks; a differentiation regeneration culture treatment is performed on the callus, the differentiated plant is continuously subjected to the secondary culture, and the callus incapable of differentiation is discarded; the stipe meristem area of the secondary culture plant after regenerating is subjected to the second callus induction, wherein the culture time is 1 month; the callus is subjected to the secondary culture for 2 months, wherein the culture media is changed every two weeks; then the differentiation regeneration culture treatment is performed on the callus, wherein the callus incapable of differentiation is discarded; a long-term secondary culture is performed on the regenerated plant, when the callus is required to carry out the relevant research, the stipe meristem area of the plant is subjected to induction and secondary culture to obtain the required callus, wherein the regenerated plant is subjected to the second differentiation. According to the present invention, the operation is simple and feasible, and the stable and high-frequency differentiation regeneration efficiency can be obtained.

The invention discloses a rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum. The method comprises acclimation of tissue culture rooting seedlings in a bottle, transplantation, environment control after transplantation, fertilization management and pest control, wherein the environment control is implemented as follows: a small arch shed is put up below a sunshade net arch structure set up in a large shed, the top surface of the small arch shed is covered with a sunshade net, and a hole tray is put in the small arch shed; the humidity of the small arch shed is kept at 80%-85%, the temperature in the daytime is kept 25 DEG C-30 DEG C, the temperature at night is kept not lower than 5 DEG C, and moisture is controlled; fertilization management and pest control are implemented as follows: after the tissue culture seedlings are transplanted to the hole tray for 30 days, a leaf fertilizer is applied once every two weeks, chlorothalonil or carbendazim is sprayed once every 15 days and sprayed for 3-4 times in total, and imidacloprid is sprayed once every week at the initial stage of aphids in spring and sprayed for 3-4 times in total. By means of the method, the transition time of the tissue culture seedlings of the rhododendron lapponicum is shortened, the survival rate of hardened seedlings is up to 90% or higher, the seedlings grow healthy and strong, a standard seedling hardening technical scheme is provided, and the method is suitable for industrial seedling hardening.

The invention discloses a taxus chinensis cuttage cultivation method. The taxus chinensis cuttage cultivation method comprises the steps as follows: inserting insertion branches treated with plant hormones onto a prepared seedbed substrate in sequence, controlling the cuttage depth to be 3-6 cm, watering each day, spraying a 700-time carbendazol solution once each week, after half a month, reducing the watering frequency, averagely watering once every 3 days, spraying the 700-time carbendazol solution once every two weeks, and transplanting for cultivating after the insertion branches take roots. The method has the advantages that firstly, as the seedbed substrate adopted in the method is better in physical permeability, formation, rooting and after-rooting growth of callus tissues are facilitated; secondly, saw powder is good in heat retaining property and the temperature difference between day and night for the substrate is slightly changed, so that rooting is facilitated; and thirdly, a small amount of humus soil is strong in water and fertility retaining capability, so that growth of root systems and new branches of nursery stocks is facilitated; the seedbed substrate is disinfected at the same time, so that the insertion branches are effectively prevented being infected with pathogenic bacteria; and the insertion branches are sprayed with the carbendazol solution after cuttage, so that the cuttage rooting is facilitated, the survival rate is increased, and the seedling growth time is shortened.

The invention discloses recombinant proserum/growth hormone fusion protein, a preparation and purification method of the recombinant fusion protein, and the use of the recombinant fusion protein to preparation of medicines for treating children dwarfism. The amino acid sequence of the recombinant proserum/growth hormone fusion protein is SEQID NO.1, and the nucleotide sequence of the recombinant proserum/growth hormone fusion protein is SEQID NO.2. According to a preparation technology of the recombinant proserum/growth hormone fusion protein disclosed by the invention, yeast engineering bacteria are constructed and expressed, so that high-density expression recombinant fusion protein is obtained; and through a purification technology, the recombinant proserum/growth hormone fusion proteinwhich can be used clinically is obtained. The recombinant proserum/growth hormone fusion protein obtained by the preparation and purification method adopts a creative medicine structure for treatingthe children dwarfism, has long residual action that administration can be performed once every two weeks, is more suitable for children medication demands, and has more excellent treatment effects, less administration frequency and lower production cost.

The invention relates inter alia to a method of treating tumorous disease in a human patient by administering to the patient a human immunoglobulin specifically binding to the human EpCAM antigen, the immunoglobulin exhibiting a serum half-life of at least 15 days, the method comprising the step of administering the immunoglobulin no more frequently than once every week, preferably no more frequently than once every two weeks.

The invention provides a corylus heterophylla Fisch.*corylus avellanaL. seedling cultivation method. The method comprises the steps that female parent tree field planting, leaf fertilizer applying and layering seedling cultivating are carried out, according to leaf fertilizer applying, when the growth height of green branches of root shoots reaches 25 cm, leaf fertilizer is irrigated in a dripped mode, the adopted amount is 5 L/mu, and spraying is carried out once every two weeks. According to the seedling cultivation method, the layering rooting rate is 99.3%, the survival rate is 99.8%, and time for seedling cultivation is shortened by 10 days; cultivated hazelnut seedlings are first-class seedlings, the seedling height is 85 cm, the ground diameter is 0.85 cm, the number of lignified side roots is 10, and the length is 25 cm; when the cultivated hazelnut seedlings grow into two-year-old trees, the freeze-resistant ability is high, and the trees can resist low temperature of 37 DEG C below zero during the dormancy period; after the cultivated corylus heterophylla Fisch.*corylus avellanaL. seedlings are planted, the fruit yield is large, the average single fruit weight reaches 2.8 g, shells are thin, the kernel percent is 45%, the average single-plant yield of the trees of 5-6 years old is 2.5 kg, and fruits are borne 10 days earlier.

The invention provides a trollflower planting method and trollflowers obtained by planting. The method mainly comprises the following steps: applying an organic fertilizer as a base fertilizer before seeding, watering the soil and turning over the soil; seeding trollflower seeds onto the soil surface, compacting, covering the surfaces of the seeds with 0.8-1.5 cm of an organic matter, enabling the sown seeds to sprout 8-9 days after seeding; growing seedlings under light all days until the seedlings grow to 15-25 cm; transplanting the seedlings to the soil laid with mulching film according to the law that 3-5 seedlings are planted in a hole within a row spacing of 50 cm*50 cm, watering in time after planting, then watering once every two weeks, applying a liquid fertilizer to the roots under mulching film every 20 days in a drop irrigation manner for three times continuously, then spraying the liquid fertilizer to foliage every 7-10 days, weeding once every 7-9 days, and cultivating until the trollflower plants grow to 1-1.2 meter high; harvesting the trollflowers 7-10 days after flowering. The trollflowers planted according to the invention are high in yield, good in quality, and large in economic and biologic benefits.

The invention discloses an ecological culture method of bait for brevifilis fry. The method comprises the following steps: A, constructing a bait pond; B, disinfecting the bait pond; C, applying fertilizer, namely, applying 8 to 12kg of special fishing fertilizer at every 667m<3>, and then compensating 8 to 12g of urea and 0.4 to 0.6g of superphosphate at each cubic meter in every two weeks; D, introducing young water earthworm, namely, draining water from the bait pond in March, uniformly spreading young water earthworm based on the standard of 0.4 to 0.6kg/m<2> to the bottom of the bait pond, and then adding water being 5cm in depth to cover the young water earthworm; E, ecologically managing, namely, adding 47-53kg of snails at every 667m<2> to control the water quality, and overturning the pond once through a rake at every 15 days; F, daily managing; and G, collecting once every 30 days at night, namely, adding cold water to the bait pond, irradiating through light to attract water earthworm to gather, and then collecting through a mesh bag formed by 40-meshes polyethylene fiber mesh cloth.

A purple grape planting method includes steps: digging a planting pit in a diameter of 60-80cm and in a depth of 60-70cm, selecting 1-2-year grafted seedlings complete in root system and large in fibril quantity, and planting by a row spacing of 1.6-1.8m and a line spacing of 5-5.5m; in a germination stage, applying 12-15kg of monoammonium phosphate and 8-10kg of urea per mu, uniformly spraying compound sodium nitrophenolate to leaf surfaces every two weeks in a flowering stage, applying 15-20kg of nitrogen fertilizers and 10-12kg of urea per mu in a fruiting stage, applying 16-18kg of potassium fertilizers and 10-12kg of urea per mu in a fruit expansion stage, and applying 20-25kg of a ternary compound fertilizer per mu 15-18 days after harvesting; harvesting in batches. The purple grapeplanting method is scientific and reasonable, fertilizer types and fertilizer quantity required by grape growth are provided so as to guarantee required nutrient elements in a purple grape growth process and promote purple grapes in good shapes, and purple grape yield is increased.

The invention discloses a rhododendron simiarum Hance seed propagation method. The method comprises steps as follows: a clay pot is filled with a mixed substrate containing humus soil and vermiculite in a volume ratio being 3:1; seeds collected in the previous year are uniformly sown on the surface of the substrate in the period from the early April to the middle of May, and then the upper layer of the seeds is uniformly covered with a sheared living moss layer; the seeds are watered sufficiently and covered with a plastic film for heat and humidity preservation, and water is replenished twice every week in a germination period; the film is uncovered after the seeds germinate, the seeds are subjected to 50%-75% sun-shade and normal-temperature maintenance management, and a fungicide is sprayed once every two weeks; seedlings after cultivated for one year are transplanted to trays for maintenance management in greenhouses, or the seedlings after cultivated for 2-3 years are directly transplanted to plastic greenhouses or outdoor nursery gardens for field management. The method is simple to operate and convenient to implement, can remarkably increase the seedling rate of rhododendron simiarum Hance, improve seedling quality, reduce difficulties in water management and disease control at the seedling stage and reduce cost, and can be applied to large-scale seedling cultivation and industrial development of the rhododendron simiarum Hance.

The invention relates to a construction method and application of a DNA (Deoxyribonucleic Acid) vaccine for avian leukosis virus subgroup J. A recombinant eukaryotic expression plasmid pcDNA3.1-Fc-env of an Fc fragment gene for expressing chicken immunoglobulin G and an envelope protein (env protein) gene of the avian leukosis virus subgroup J is constructed; transient transfection and indirect immunofluorescence assay prove that the pcDNA3.1-Fc-env can be accurately expressed in a 293T cell; a great number of plasmids are extracted, purified and quantified to 1mg/ml, then, the recombinant plasmids are used for immunizing mice, each mouse is immunized three times, 100mu g of recombinant plasmids are used in each immunization, and one immunization is carried out every two weeks; the level of an ALV-J env protein-specific antibody in serum is detected to show that the pcDNA3.1-Fc-env has the effect of preventing the avian leukosis virus subgroup J.

The invention discloses a method for planting blueberry. The method for planting blueberry comprises: first, the land of blueberry is began with a long hill and the blueberry distance and spacing are determined; second, a drain is set; third, soil is mixed with manure and the manure is prepared from, by weight: 100-120 parts of shard, 5-6 parts of sulphur powder, 150-180 parts of distillers' grains, 50-60 parts of bits of wood, 20-30 parts of dough cake and 2-3 parts of enzyme microorganism, acres of manure 400- 600 kilogram; forth, planted blueberry is poured on root, 10-20 thick pine needles are laid in the soil on the long hill and pine needles are covered with coconut bran 5 - 10 centimeter thick; the fifth is water application: during the time of blueberry cultivation, soil moisture content is kept in 20 - 25 percent; the fifth also includes spreading manure: after two moths blueberry planting, biogas slurry is sprayed to blueberry leaves until the buds appear, once every two weeks; buds appearing and half month before blueberry harvest, nutrient solution is sprayed on blueberry leave once a week, and the nutrient solution is prepared from, by weight: 10-15 parts of biogas slurry and 60-70 parts of vinegar lees. The method for planting blueberry improves the yield and quality of blueberry by using reasonable planting method.

The invention discloses a health-protecting growth-promoting feed additive for duck and application of the feed additive. The additive is prepared from the following ingredients: tropaeolum, laccocephalum mylittae, fructus momordicae, ailanthus altissima, endive, flabelate maiden-hair herb, puffball, chrysanthemum, sweet wormwood, astragalus smicus, polygonum caespitosum and pumpkin seeds, and is prepared by the blending the ingredients according to a proportion. When in use, the weight of the additive is 1.5-3.5% of weight of the basal feed, and the duck is fed once every two weeks, and continuously fed for two days once; in epidemic situation, the dosage is increased to 3.5%, and the additive is continuously fed for 3 days. The average weight increment in the test groups using the additive at 70 days old is higher than that in the blank control group, while the morbidity is lower than that in the blank control group. The health-protecting growth-promoting feed additive is based on physiologic characteristics of disease occurrence regularity of the duck, meanwhile considers about the compatibility of traditional Chinese medicines, and has the effects of preventing and controlling the disease, promoting growth and the like as the medicines in each ingredient are mutually synergetic and added.

The invention discloses a growing and fattening pig healthcare growth-promoting feed additive and application thereof. The additive comprises the following components: common nostoc, honeysuckle leaves, herba portulacae, polygala tenuifolia, watermelon roots and leaves, maidenhair, ashycoloured ironweed herb, cynanchum amplexicaule, melastoma affine, narrowleaf falsepimpernel herb, polygonum posumbu and plant soot, wherein the components are blended according to ratios. When being used, the additive accounts for 1.5-3.5% of basic feed in mass and used once for two days continuously every two weeks; when diseases break out, the dosage is increased to be 3.5%, and the additive is fed for 3 days continuously. The average weight increase of experiment groups using the additive disclosed by the invention is larger than that of a blank control group, and the morbidity of the experiment groups is lower than that of the blank control group. According to the physiological characteristics and the disease regularity of outbreak of pigs as well as the compatibility principle of traditional Chinese medicines, the medicines cooperate with one another and promote and enhance one another, so that the additive has the effects of disease prevention, disease treatment, growth promoting and the like.

The invention discloses a mist cuttage and rapid propagation method for Sinocrassula leaves. The method comprises cultivation of a female parent, leaf stripping, wound healing, preparation of a cuttage bed, leaf cuttage and management, and plug seedling management. In the cultivation of the female parent, a soilless cultivation way is adopted; the temperature is kept at 20-30 DEG C in the daytime and at 10-16 DEG C at night; the relative air humidity is 55-65 percent; and a compound fertilizer is applied once every two weeks. Cuttage leaves are placed into a greenhouse of which the shading coefficient is 80-85 percent for airing till base wounds of the leaves are healed. Cinerite is taken as a seedbed cuttage substrate. The temperature in a leaf cuttage shed is kept at 20-30 DEG C in the daytime and at 12-16 DEG C at night, and the relative air humidity is kept at 80-90 percent. The leaves are transplanted after rooting, and turf and cinerite are taken as substrates. The temperature and humidity in a plug seedling shed are the same as those in the female parent cultivation. Through adoption of the method, a cultivated female parent is robust; cuttage leaves take root quickly; from cuttage to rooting and seedling emergence, 35-42 days are spent; from cuttage to the formation of commercial seedlings, 45-57 days are spent; and the cuttage seedling rate is over 96 percent. The method is suitable for large-scale seedling production.

The present invention concerns a pediatric growth hormone deficiency (PGHD) therapy for pediatric subjects. The therapy comprises administering to the pediatric patient with PGHD a human growth hormone-XTEN (hGH-XTEN) fusion protein in therapeutically effective doses every week, every two weeks, semimonthly, every three weeks, or monthly. This therapy is not inferior compared to the height velocity achieved with daily injections of hGH not linked to XTEN over the same period.

The invention discloses a culture method for effectively reducing the heavy metal cadmium content in cultured fish bodies. A mixed culture method oriented to fish and pearl mussel culture is adopted, and 70-100 kg of quick lime is applied for each mu of water surface before putting; in the culture process, the quick lime is applied for one time every two weeks, and the application amount each time is appropriately adjusted according to the season and weather conditions. By the adoption of the culture method, the content of cadmium ions entering cultured fish bodies or other aquatic organism bodies can be effectively reduced; the pH of a culture water body is regulated through the quick lime, and the toxic effect of the heavy metal cadmium on the cultured fishes is reduced; a sufficient calcium source ensures healthy development of bones and other components of the fishes and other aquatic organisms; the stronger heavy metal enrichment capabilities of hyriopsis cumingii and other low-value mussels are fully utilized, and the amount of the heavy metal cadmium enriched in the fish bodies can be decreased. The culture method has the advantages of being simple, simple and convenient to operate, wide in raw material source, low in cost, obvious in effect and convenient to popularize and apply during production.

The invention relates to the technical field of deratization, in particular to a deratization method. The deratization method comprises the following steps: (1) putting in rat poisons: placing rat houses in rat usually haunting places and putting in the rat poisons; (2) pasting warning signs: arranging the warning signs on the rat houses; (3) cleaning the rat houses: cleaning the rat houses once every two weeks after the rat poisons are put in; (4) blocking rat holes: blocking hole openings of rat caves by using cement or concrete. According to the deratization method disclosed by the invention, the rat houses are placed in the rat usually haunting places to trap and kill rats, and rat dead bodies are cleaned timely to prevent infection of germs from resulting in diseases, furthermore, the rat holes are blocked to prevent the rats from continuously harming buildings, so that the purpose of effectiveness, efficiency, safety, and no pollution of the deratization process is realized.

The invention discloses a propagation method of dendrobiumchrysotoxumlindlphanerophyte. The method comprises the step of (1), budding phanerophyte, namely spraying nutrient solution on whole plant of the dendrobiumchrysotoxumlindl for one time every two weeks from mild-to-end of February to March in Spring, in which the nutrient solution is prepared adding carbamide into a biogas slurry and controlling content of nitrogen in the nutrient solution to be 0.05-0.15%; (2) cutting phanerophyte, namely cutting the phanerophyte and 1-2 sections of stock plant bulbus commonly when root grows to 2-4cm, dipping cutting notches in 600-800-fold solution of 70% mancozebwettable powder for 3-5 seconds, and then using moss to wrap the root and plant the root in a planting bed swallet; (3) performing initial management of phanerophyte seedlings; and (4) performing final-period management of the phanerophyte seedlings. Through the method, a large number of phanerophytes can be germinated; a germination rate is 80-90%; rate of survival of the phanerophyte seedlings is greater than or equal to 98%; the phanerophyte seedlings grow quickly and can flower in about 2 years; and yield of flowers reaches 80-110kg/unit of area.

An ecological cultivation method of fugu flavidus intercropping barracuda parental fish is characterized in that barracudas are released to prepare parental fish in winter; an outdoor earth pond is provided with an aerator of 1.5 Kw at every 3 to 5 mu; the pond is disinfected by quick lime before the barracudas are released; water is injected into the pond a week later and the water source is natural seawater; after ten days of injecting the water, two-year-old barracudas are released to prepare parental fish as backup; the density is within the range of 20 to 30 tails per mu without feeding fish meal; in spring, the water temperature of the outdoor earth pond is risen to 14 to 15 DEG C; two-year-old fugu flavidus fingerlings that live through winter are released into the outdoor earth pond; the density is within the range of 800 to 1000 tails per mu; the fugu flavidus fingerlings are fed with baits twice a day; the daily bait-feeding amount is 3 to 8 percent of the weight of each of the fugu flavidus fingerlings; the baits are eel feeding stuff; the next bait-feeding amount is regulated timely according to the ingestion situation; the water is changed once every two weeks; from mid to late of November, the fugu flavidus fingerlings are started to be caught into a greenhouse for living through winter; and the barracudas are kept in the pond to early January and the pond is dried for catching the barracudas when the temperature is relatively low.

The invention provides a rice, shrimp and soft-shelled turtle symbiotic efficient low-carbon agriculture breeding method. The method includes the following steps that a, a good rice field is selectedas a rice, shrimp and soft-shelled turtle symbiotic field parcel, rice is planted in the center of the rice field, annular furrows are dug in the periphery of the rice field for breeding shrimps and soft-shelled turtles, and anti-escape facilities are built on the peripheries of the annular furrows; b, the annular furrows start to be cleaned and aired in the last ten days of February, medicine pond cleaning is carried out on the annular furrows, and disinfection with bleaching powder is carried out after water flows; c, water is stored, basic bait is cultured, 10 cm-20 cm of water flows again,organic fertilizer is selected for water full of fertilizer, and 3 kg of nitrogen fertilizer and 1 kg of phosphatic fertilizer are applied per mu; d, 180-200 kg of margarya melanoide starts to be putper mu in the middle ten days of March; e, young shrimps are put in the first ten days of April; f, aquatic plants start to be transplanted in the middle ten days of April; g, rice seedling raising is carried out in the middle ten days of May; h, microbial preparations start to be put every two weeks in the middle ten days of May; i, soft-shelled turtle fry are stocked in the last ten days of May, and dry-pond catching is carried out in the last ten days of November; j, rice seedling transplanting is carried out in the first ten days of June, and rice is harvested in the first ten days of November. By means of the method, use of chemical fertilizer is restrained, the input-output ratio is increased, and incomes of farmers are increased.