123 results about "Avian leukosis viruses" patented technology

The invention relates to a kit for detecting the specific antibody of the A/ B/ J subgroup of an avian leukosis virus and a detection method thereof, and belongs to the field of molecular biology. Thekid includes a luminescent microporous plate, a sealing film, a receptor microsphere I, a receptor microsphere II, a donor microsphere, a calibrator and an analytical buffer solution; the receptor microsphere I is marked with the His-cENV protein of the avian leukosis virus A/ B subgroup, and the receptor microsphere II is marked with the His-gp85 protein of the avian leukosis virus J subgroup; the donor microsphere is marked with an rabbit-anti-chicken second-antibody. The detection method includes the steps of firstly, preprocessing a collected sera sample; then, adding the receptor microsphere I, the receptor microsphere II and the sera sample into the luminescent microporous plate as required, and detecting an optical signal value under 680 nm exciting light. The method achieves the detection on the A/ B/ J specific antibody of the avian leukosis virus, and has the advantages of no washing, simple operation, sensitive detection, high specificity and strong stability.

The invention discloses a CQD (Carbon Quantum Dot) adjuvant and a vaccine containing the same, i.e., each single part of vaccine agent contains 200 to 400mug of the CQD adjuvant. The phenomenon that CQDs can be used as an excellent adjuvant for resisting ALV-J (avian leukosis virus subgroup J) is found for the first time; researches find that the CQDs prepared by the invention have no cytotoxicity, cannot induce reduction of the cell activity, are good in biocompatibility, and are safe and reliable to use in the immunizing dose range. The vaccine prepared by using the CQDs as the adjuvant, which is disclosed by the invention, with respect to a vaccine prepared by a Freund's adjuvant, can more effectively induce the generation of a high antibody titer to carry out immune protection and provide a more lasting protective immune response.

The invention relates to a method for efficiently amplifying subgroup J avian leukosis virus (ALV). According to the method, chicken hepatocytes can be used for efficiently amplifying the subgroup J ALV, the characteristic of high titer virus can be produced earlier by using the chicken hepatocytes, the separation and detection time of the subgroup J ALV can be shortened, the pathogen can be detected earlier, and the sensitivity of a subgroup J ALV detection method is improved. When subgroup J ALV infection MOI is 0.001 hour, the effect of enzyme-linked immunosorbent assay (ELISA) on the chickhepatocytes infected for 3 days is comparable to the effect of ELISA on DF-1 cells infected for 7 days. The efficient amplification method established by the invention accelerates the purification process of the subgroup J ALV, thus shortening the time and reducing cost; the method has very good application value in the purification detection of the subgroup J ALV.