15 results about "Molecular Immunology" patented technology

The invention relates to the technical field of molecular immunology and biology, in particular to a sicca syndrome model mouse B cell immune repertoire sequencing and subpopulation analysis method. The method comprises the following steps: S1, sample preparation: preparing a single-cell suspension from a target autoimmune disease animal model and a preset tissue of a control animal, and carrying out B cell enrichment treatment on the single-cell suspension to obtain a B cell sample; s2, multi-dimensional data parallel analysis: analyzing the B cell sample, and performing parallel construction of the multi-dimensional data; and S3, integrated correlation analysis: carrying out correlation analysis on the immune group library data, the cell subset data and the signal channel data obtained in the step S2 to establish a correlation relationship among specific B cell cloning characteristics, specific B cell subset changes and specific signal channel activity, and carrying out multilevel analysis and generating a B cell immune response mechanism of the autoimmune disease. The efficiency and accuracy of disease mechanism research and drug action mechanism evaluation are remarkably improved.

The application discloses an African swine fever H240R epitope peptide, a monoclonal antibody and application thereof, and relates to the technical field of molecular immunology. The amino acid sequence of the African swine fever H240R epitope peptide is shown as SEQ ID NO. 19 or SEQ ID NO. 20. The monoclonal antibody is monoclonal antibody 11C1A6 or monoclonal antibody 38A3B2; the amino acid sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody 11C1A6 are shown as SEQ ID NO. 9-10, respectively; the amino acid sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody 38A3B2 are shown as SEQ ID NO. 11-12, respectively. The African swine fever H240R epitope peptide is identified, and the monoclonal antibody which is specifically combined with the African swine fever H240R protein is prepared, thereby laying a foundation for preparing an African swine fever polypeptide vaccine and a detection kit.

The present invention relates to the fields of treating autoimmune diseases and molecular immunology, and specifically, to an anti-IL-17A antibody, a pharmaceutical composition thereof, and use thereof. The present invention relates to a monoclonal antibody or an antigen binding fragment thereof, wherein a heavy chain variable region of the monoclonal antibody comprises: HCDR1-HCDR3 with amino acid sequences set forth in SEQ ID NOs: 31-33 respectively, or HCDR1-HCDR3 with amino acid sequences set forth in SEQ ID NOs: 37-39 respectively, and a light chain variable region of the monoclonal antibody comprises: LCDR1-LCDR3 with amino acid sequences set forth in SEQ ID NOs: 34-36 respectively, or LCDR1-LCDR3 with amino acid sequences set forth in SEQ ID NOs: 40-42 respectively. Monoclonal antibodies of the present invention can specifically antagonize the binding of IL-17A to a ligand and inhibit activation of fibroblasts by IL-17A.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-5C8, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

This invention relates to monoclonal antibodies against CTLA4 or their antigen-binding fragments, pharmaceutical compositions, and uses. The invention belongs to the fields of tumor therapy and molecular immunology, providing monoclonal antibodies against CTLA4 or their antigen-binding fragments, pharmaceutical compositions thereof, and their uses. The monoclonal antibodies of this invention can block the binding of CTLA4 to B7, relieve the immunosuppression caused by CTLA4, and activate T lymphocytes.

This invention belongs to the field of zoonotic disease prevention and control and molecular immunology technology, specifically relating to the application of a biological product that silences or knocks out the ID2 gene in regulating host resistance to Brucella infection. This invention constructs an ID2 gene knockout macrophage model using CRISPR / Cas9 gene editing technology and establishes a Brucella infection system. Experiments show that ID2 gene knockout significantly enhances the host's ability to clear Brucella. RNA-seq analysis shows that ID2 participates in regulating the host's transcriptional response network to Brucella, with its differentially expressed genes significantly enriched in lipid metabolism and autophagy. Mechanistic results indicate that the ID2 gene participates in the regulation of Brucella-mediated lipophage. It can be seen that the ID2 gene affects the intracellular replication level and lipophage process of Brucella. This invention provides new targets and theoretical basis for drug development against Brucella infection, immune regulation, and disease-resistant breeding.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-1A1, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The application discloses a duck BRS3 polypeptide and a preparation method and application of a polyclonal antibody thereof, and belongs to the field of biochemistry and molecular immunology. The amino acid sequence of the duck BRS3 polypeptide is shown as SEQ ID NO. 3. A polypeptide sequence with a length of 20 aa is screened from an intracellular region of a duck BRS3 protein sequence, and after being artificially synthesized by a solid phase, the polypeptide sequence is used as an immunizing antigen to immunize New Zealand white rabbits, and a duck BRS3 protein polyclonal antibody with an antiserum titer of 1:25600 or above is obtained. The rabbit anti-duck BRS3 polyclonal antibody prepared in the application can specifically combine with the BRS3 protein in duck tissues, fills a blank in the field of duck BRS3 protein detection research, and lays a foundation for the research on the function of BRS3 in a duck body.

This invention discloses a method for preparing and applying a duck interneuron B modified polypeptide and its polyclonal antibody, belonging to the fields of biochemistry and molecular immunology. The amino acid sequence of the duck interneuron B modified polypeptide is shown in SEQ ID NO.4. This invention screens a 19aa polypeptide sequence from the duck interneuron B protein sequence, adds a cysteine ​​residue (Cys, C) to its C-terminus, synthesizes it artificially in a solid phase, and uses it as an immunogenic antigen to immunize New Zealand white rabbits, obtaining a duck interneuron B polyclonal antibody with an antiserum titer of over 1:204800. The rabbit anti-duck interneuron B polyclonal antibody prepared by this invention can specifically bind to interneuron B protein in duck tissues, filling a gap in the research field of duck interneuron B protein detection and laying the foundation for the study of the function of interneuron B in ducks.

The application discloses a novel coronavirus S protein specific B cell antigen epitope polypeptide and application thereof, and belongs to the technical field of molecular immunology. In order to provide a novel coronavirus protein polypeptide antigen with immunoprotective function or serodiagnosis value. The amino acid sequence of the antigen epitope polypeptide disclosed by the application is shown as SEQ ID NO:1 or SEQ ID NO:2. The SARS-CoV-2 S protein specific B cell antigen epitope synthetic polypeptide, the synthetic epitope polypeptide coupling antigen and the antigen epitope fusion expression protein disclosed by the application can be used as antigens to specifically detect the anti-SARS-CoV-2 S protein antibodies generated after the body is immunized or infected, can be used for SARS-CoV-2 infection diagnosis, vaccine immunization effect evaluation and as a vaccine component to induce specific antibodies.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-6D6, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The application belongs to the technical field of molecular immunology, and particularly relates to a kind of anti-GAPDH carboxyethylation modified monoclonal antibody and its application, the amino acid sequence of HCDR1~HCDR3 of the monoclonal antibody is sequentially shown as SEQ ID NO.4~SEQ ID NO.6;The amino acid sequence of LCDR1~LCDR3 is sequentially shown as SEQ ID NO.7~SEQ ID NO.9.The application successfully prepared the monoclonal antibody of specific recognition GAPDH Cys247 carboxyethylation modification, the antibody has high affinity and high specificity, can effectively distinguish the carboxyethylation similar to lactoylation modification.The antibody can be applied to immunoblotting, immunofluorescence, immunoprecipitation and other various detection scenes, and can be used for preparing inflammation diagnostic kit and screening anti-inflammatory drugs, has the dual value of basic research and clinical application.

The present application belongs to the field of fish molecular immunology, and particularly relates to an oral vaccine containing a largemouth bass iridovirus ATPase and a preparation method thereof. B. subtilis The spores of the WB600 strain are antigen delivery carriers, the spore coat protein C (CotC) is used as an anchor protein, and the key protein of the largemouth bass iridovirus is fused with the ATPase gene and CotC the gene to construct a recombinant spore surface display system, so that the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. The vaccine can tolerate the gastrointestinal environment after being orally administered to fish, and is accurately delivered to the gut-associated lymphoid tissue to stimulate the immunity of the fish, so that the prevention and control of the largemouth bass iridovirus is finally achieved.

The application discloses a Paralichthys olivaceus rhabdovirus recombinant single-chain variable region antibody and application thereof, and belongs to the field of molecular immunology. The recombinant single-chain variable region antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application is characterized in that the G protein B cell antigen epitope of the Paralichthys olivaceus rhabdovirus G protein gene is obtained through precise screening, the epitope sequence is used as an antigen to prepare a hybridoma cell, and the specific single-chain antibody is efficiently produced by using an E. coli expression system. The recombinant single-chain antibody can specifically recognize and combine with HIRRV, targets a key functional region of the virus, effectively neutralizes virus infection, reduces non-specific immune responses, and significantly improves the accuracy of rapid diagnosis, detection and treatment. The application can be used as a Paralichthys olivaceus rhabdovirus infection detection or diagnosis reagent and a targeted blocking drug for Paralichthys olivaceus rhabdovirus.