42 results about "Molecular Immunology" patented technology

The invention discloses a paralichthys olivaceus rhabdovirus G protein tandem antigen epitope peptide and application thereof, and belongs to the field of fish molecular immunology. The amino acid sequence of the tandem antigen epitope peptide is shown as SEQ ID NO: 1. The preparation method comprises the following steps: (1) firstly, analyzing structural characteristics of HIRRV-G protein, predicting B cell antigen epitopes of the HIRRV-G protein, screening the antigen epitopes with advantages on the basis of a prediction result, and synthesizing the antigen epitopes; (2) screening a candidate peptide fragment with high affinity through an enzyme-linked immunosorbent assay; and (3) sequentially connecting the high-affinity peptide fragment sequences meeting the requirements by using a GPGPG connexon, cloning the connected sequences into a pET-28a prokaryotic expression vector, and performing induced expression to obtain the tandem antigen epitope peptide. Compared with a full-length G protein, the tandem antigen epitope peptide is smaller in molecular weight, higher in stability and higher in hydrophilicity; a large number of specific antibodies can be induced in fish bodies, and the death rate of the paralichthys olivaceus infected by viruses is remarkably reduced. The method can be used for HIRRV diagnosis detection reagent and subunit vaccine development.

The present invention relates to the field of immunotherapy and molecular immunology, and in particular to a monoclonal antibody specifically binding to PD-1 or an antigen-binding fragment thereof, and a pharmaceutical use thereof. The antibody or the antigen-binding fragment thereof can be particularly used for preventing or treating cancers, inflammation, and autoimmune diseases.

The invention discloses a duck BRS3 polypeptide and a preparation method and application of a polyclonal antibody of the duck BRS3 polypeptide, and belongs to the field of biochemistry and molecular immunology. The amino acid sequence of the duck BRS3 polypeptide is as shown in SEQ ID NO. 3. A segment of polypeptide sequence with the length of 20 aa is screened out in an intracellular region of a duck BRS3 protein sequence, after artificial solid-phase synthesis, the polypeptide sequence is used as an immunizing antigen to immunize New Zealand white rabbits, and the duck BRS3 protein polyclonal antibody with the antiserum titer being 1: 25600 or above is obtained. The rabbit anti-duck BRS3 polyclonal antibody prepared by the invention can be specifically combined with the BRS3 protein in duck tissues, so that the blank in the field of detection and research of the duck BRS3 protein is filled, and a foundation is laid for research of functions of the BRS3 in duck bodies.

The application discloses a tandem antigen epitope peptide of G protein of Paralichthys olivaceus rhabdovirus and application thereof, and belongs to the field of fish molecular immunology. The amino acid sequence of the tandem antigen epitope peptide is shown as SEQ ID NO:1. The preparation method of the application comprises the following steps: (1) first, analyzing the structural characteristics of HIRRV-G protein and predicting B cell antigen epitopes, and synthesizing the antigen epitopes with advantages based on the prediction results; (2) then, screening candidate peptide segments with high affinity through enzyme-linked immunosorbent assay; (3) sequentially connecting the high-affinity peptide segment sequences meeting the requirements by using a GPGPG linker, and cloning the connected sequences into a pET-28a prokaryotic expression vector, so that the tandem antigen epitope peptide is obtained after induced expression. Compared with the full-length G protein, the tandem antigen epitope peptide has smaller molecular weight, stronger stability and stronger hydrophilicity; the tandem antigen epitope peptide can induce a large amount of specific antibodies in fish bodies, and significantly reduces the mortality of fish infected by the virus. The tandem antigen epitope peptide can be used for the development of HIRRV diagnostic reagents and subunit vaccines.

The invention discloses a paralichthys olivaceus rhabdovirus recombinant single-chain variable region antibody and application thereof, and belongs to the field of molecular immunology. The recombinant single-chain variable region antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. According to the present invention, paralichthys olivaceus rhabdovirus G protein gene is cloned, accurate screening is performed to obtain the B cell antigen epitope of the G protein, the hybridoma cell is prepared by using the epitope sequence as the antigen, and the specific single-chain antibody is efficiently produced by using the Escherichia coli expression system; the recombinant single-chain antibody can specifically recognize and combine with HIRRV, target a virus key functional region, effectively neutralize virus infection, reduce non-specific immunoreaction, and significantly improve the accuracy of rapid diagnosis, detection and treatment. The compound can be used as a paralichthys olivaceus rhabdovirus infection detection or diagnosis reagent and a paralichthys olivaceus rhabdovirus targeted blocking drug.

The present invention relates to the technical field of molecular immunology, and specifically discloses novel antigen polypeptides for lung cancer and their applications. The polypeptide comprises one or more amino acid sequences selected from those shown in, for example, PDGPPEKP (SEQ ID NO.1), MNKALLPAK (SEQ ID NO.2), and MYCELPDSF (SEQ ID NO.3); or is a polypeptide with the same or similar function obtained by substituting and / or deleting and / or adding at least one amino acid in the amino acid sequences of the above polypeptides. The novel antigen polypeptide for lung cancer provided by the present invention can bind to MHC class I molecules on human cells, can significantly stimulate the activation and expansion of T lymphocytes with tumor cell killing effects, and increase the killing ability of T cells against lung cancer cells. Therefore, the polypeptide can be used to prepare drugs for treating lung cancer, especially for preparing lung cancer cell vaccines. The antigen polypeptide can be used to treat lung cancer and prevent recurrence and metastasis in patients after surgery.

The invention relates to the technical field of molecular immunology and biology, in particular to a sicca syndrome model mouse B cell immune repertoire sequencing and subpopulation analysis method. The method comprises the following steps: S1, sample preparation: preparing a single-cell suspension from a target autoimmune disease animal model and a preset tissue of a control animal, and carrying out B cell enrichment treatment on the single-cell suspension to obtain a B cell sample; s2, multi-dimensional data parallel analysis: analyzing the B cell sample, and performing parallel construction of the multi-dimensional data; and S3, integrated correlation analysis: carrying out correlation analysis on the immune group library data, the cell subset data and the signal channel data obtained in the step S2 to establish a correlation relationship among specific B cell cloning characteristics, specific B cell subset changes and specific signal channel activity, and carrying out multilevel analysis and generating a B cell immune response mechanism of the autoimmune disease. The efficiency and accuracy of disease mechanism research and drug action mechanism evaluation are remarkably improved.

The application discloses an African swine fever H240R epitope peptide, a monoclonal antibody and application thereof, and relates to the technical field of molecular immunology. The amino acid sequence of the African swine fever H240R epitope peptide is shown as SEQ ID NO. 19 or SEQ ID NO. 20. The monoclonal antibody is monoclonal antibody 11C1A6 or monoclonal antibody 38A3B2; the amino acid sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody 11C1A6 are shown as SEQ ID NO. 9-10, respectively; the amino acid sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody 38A3B2 are shown as SEQ ID NO. 11-12, respectively. The African swine fever H240R epitope peptide is identified, and the monoclonal antibody which is specifically combined with the African swine fever H240R protein is prepared, thereby laying a foundation for preparing an African swine fever polypeptide vaccine and a detection kit.

The present invention relates to the field of tumor therapy and molecular immunology, relates to a pharmaceutical use of an anti-PD-1-anti-VEGFA bispecific antibody, and in particular, to a use of the anti-PD-1-anti-VEGFA bispecific antibody or a pharmaceutical combination of said antibody and a chemotherapy drug in the preparation of a drug for treating non-small cell lung cancer with brain metastases. The bispecific antibody can effectively treat non-small cell lung cancer with brain metastases, has high safety, and has good prospects of application.

The invention belongs to the field of tumor treatment and molecular immunology, and relates to medical application of an anti-VEGFA-anti-PD-1 bispecific antibody. Specifically, the invention relates to application of an anti-VEGFA-anti-PD-1 bispecific antibody or a pharmaceutical composition of the anti-VEGFA-anti-PD-1 bispecific antibody and chemotherapeutic drugs in preparation of drugs for treating non-small cell lung cancer brain metastasis. The bispecific antibody can effectively treat non-small cell lung cancer brain metastasis, is high in safety and has a good application prospect.

The invention discloses a duck GRPR polypeptide and a preparation method and application of a polyclonal antibody of the duck GRPR polypeptide, and belongs to the field of biochemistry and molecular immunology. The duck GRPR gene is cloned for the first time, and the structure of the duck GRPR gene is analyzed; the preparation method comprises the following steps: taking amino acid polypeptide at 350-368 sites of a carboxyl terminal of duck GRPR protein as a hapten, and carrying out N-terminal modification on a polypeptide sequence to obtain duck GRPR modified polypeptide; and coupling the modified polypeptide with a carrier protein keyhole limpet hemocyanin to prepare a complete antigen, immunizing an animal, and separating and purifying from antiserum to obtain the polyclonal antibody of the duck GRPR polypeptide. Experiments show that the polyclonal antibody can be specifically combined with GRPR protein in duck tissue. According to the invention, the blank in the field of duck GRPR protein detection and research is filled, and a foundation is laid for research of the functions of the GRPR in the duck body.

The application discloses application of an Aeromonas vancleavei OmpW subunit vaccine as a vaccine for preventing bacterial septicemia of carps, and belongs to the technical field of genetic engineering and molecular immunology. The subunit vaccine protein is encoded by an Aeromonas vancleavei OmpW gene, the base sequence of the Aeromonas vancleavei OmpW gene is shown in the sequence table SEQ ID No. 1, the encoded protein is an Aeromonas vancleavei outer membrane protein OmpW subunit vaccine protein, and the amino acid sequence of the Aeromonas vancleavei outer membrane protein OmpW subunit vaccine protein is shown in the sequence table SEQ ID No. 4. After the subunit vaccine of the application is injected into carps, the fish body can be significantly stimulated to up-regulate expression of immune-related genes and produce a large amount of specific antibodies, the ability of the carps to resist Aeromonas vancleavei infection is effectively improved, and then bacterial septicemia of carps caused by Aeromonas vancleavei in carp culture is prevented.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-5C8, and the monoclonal antibody for resisting haemocyanin of Chinese prawns can be expressed through recombinant Expi293 cells of recombinant plasmids pcDNA3.1 containing genes of the monoclonal antibody for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The invention discloses a duck NMBR polypeptide, a polyclonal antibody and application, and belongs to the field of biochemistry and molecular immunology. The duck NMBR gene is cloned for the first time, and the structure of the duck NMBR gene is analyzed; amino acids at 341-359 sites at the carboxyl terminal of duck NMBR protein are used as haptens, the haptens are coupled with keyhole limpet hemocyanin to prepare complete antigens, then animals are immunized, and the polyclonal antibody of the duck NMBR polypeptide is separated and purified from antiserum. Experiments show that the polyclonal antibody can specifically bind to NMBR protein in duck tissue. According to the invention, the blank in the field of duck NMBR protein detection and research is filled, and a foundation is laid for research of the function of the NMBR in the duck body.

The invention discloses an anti-lipopolysaccharide factor of procambarus clarkia and antibacterial application of a core antibacterial structural domain of the anti-lipopolysaccharide factor, and belongs to the technical field of genetic engineering and molecular immunology. According to the technical scheme, the gene sequence of the procambarus clarkia anti-lipopolysaccharide factor is shown as SEQ ID No.1 and SEQ ID No.4 in a sequence table. The invention further specifically discloses an expression method of prokaryotic protein of the procambarus clarkia anti-lipopolysaccharide factor and an identification and synthesis method of a core antibacterial structural domain. The aeromonas hydrophila and the procambarus clarkii can be used for remarkably killing the aeromonas hydrophila in vivo and in vitro, and the survival rate of the procambarus clarkii infected with the aeromonas hydrophila can be remarkably improved. The anti-lipopolysaccharide factor and the core antibacterial structural domain thereof are stable in structure, simple to prepare, definite in component, safe and stable, and can effectively prevent the procambarus clarkii from death caused by bacterial infection.

The present invention relates to the gene, protein, recombinant expression vector, recombinant bacterium and application of fish interferon, belonging to the technical field of fish molecular immunology. The cDNA nucleotide sequence of the gene of fish interferon is as shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by the gene is as shown in SEQ ID NO.2. The present invention constructs an engineering strain to obtain a recombinant protein of fish interferon in vitro, and finds that it can significantly improve the phagocytic ability of macrophages of humpback groupers. Moreover, after overexpressing or interfering with the expression of the gene of humpback grouper interferon, it is found that it can significantly enhance the immune function of humpback groupers against Vibrio harveyi infection, and can be developed into an immunopreparation, having good practical application value.

The application provides a liquid-phase probe-based cfDNA-TCR library construction analysis method and application thereof, and belongs to the field of molecular immunology, and comprises the following steps: plasma cfDNA extraction, cfDNA UMI-UDI library construction, liquid-phase probe hybridization capture, enrichment of cfDNA library containing TCR genes, second-generation sequencing, off-machine data filtering and cleaning, merging of double-end sequencing data, alignment of sequencing data to a reference genome, removal of non-specific capture sequences, PCR deduplication according to UMI molecules, input of data into TRUST4 for contig assembly and annotation, and further immune repertoire analysis according to annotated CDR3 results. According to the method, TCR molecules in cfDNA can be more accurately detected by distinguishing PCR repeats and cloning repeats.

The present invention relates to the fields of treating autoimmune diseases and molecular immunology, and specifically, to an anti-IL-17A antibody, a pharmaceutical composition thereof, and use thereof. The present invention relates to a monoclonal antibody or an antigen binding fragment thereof, wherein a heavy chain variable region of the monoclonal antibody comprises: HCDR1-HCDR3 with amino acid sequences set forth in SEQ ID NOs: 31-33 respectively, or HCDR1-HCDR3 with amino acid sequences set forth in SEQ ID NOs: 37-39 respectively, and a light chain variable region of the monoclonal antibody comprises: LCDR1-LCDR3 with amino acid sequences set forth in SEQ ID NOs: 34-36 respectively, or LCDR1-LCDR3 with amino acid sequences set forth in SEQ ID NOs: 40-42 respectively. Monoclonal antibodies of the present invention can specifically antagonize the binding of IL-17A to a ligand and inhibit activation of fibroblasts by IL-17A.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-5C8, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits with molecular weights of 73kDa and 75kDa of Chinese shrimp hemocyanin, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention relates to the technical field of molecular immunology of prawns, in particular to a monoclonal antibody for resisting haemocyanin of Chinese prawns and application, the monoclonal antibody for resisting haemocyanin of Chinese prawns is anti-SHC-6D6 and can be expressed through a recombinant Expi293 cell of a recombinant plasmid pcDNA3.1 containing a monoclonal antibody gene for resisting haemocyanin of Chinese prawns; the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin is good in specificity, can be specifically combined to two subunits with the molecular weights of 73kDa and 75kDa of the fenneropenaeus chinensis haemocyanin, and can be used as a positive internal reference for detecting the content of the monoclonal antibody for resisting the fenneropenaeus chinensis haemocyanin in animals immunized by the fenneropenaeus chinensis haemocyanin.

The application discloses a duck hepatitis A virus type 3 VP0 polypeptide and a preparation method and application of a polyclonal antibody thereof, and belongs to the technical field of biochemistry and molecular immunology. The duck hepatitis A virus type 3 VP0 polypeptide has an amino acid sequence as shown in SEQ ID NO. 2. A polyclonal antibody is prepared by immunizing Kunming mice with the polypeptide. The polyclonal antibody of the duck hepatitis A virus type 3 VP0 polypeptide is obtained by immunizing an antiserum with a VP0 polypeptide coupled with KLH as an antigen. The polyclonal antibody preparation method is simple, low in cost, high in antibody titer, and capable of specifically recognizing the duck hepatitis A virus type 3 VP0 protein in eukaryotic cells.

The application relates to the technical field of shrimp molecular immunology, and in particular to an anti-Chinese shrimp hemocyanin monoclonal antibody and application. The anti-Chinese shrimp hemocyanin monoclonal antibody is anti-SHC-14B3, which can be expressed by recombinant Expi293 cells containing a recombinant plasmid pcDNA3.1 of the anti-Chinese shrimp hemocyanin monoclonal antibody gene. The anti-Chinese shrimp hemocyanin monoclonal antibody has good specificity, can be specifically combined with two subunits of Chinese shrimp hemocyanin with molecular weights of 73 kDa and 75 kDa, and can be applied as a positive internal reference for detecting the content of the anti-Chinese shrimp hemocyanin monoclonal antibody in animals immunized with Chinese shrimp hemocyanin.

The invention provides a construction and analysis method of a cfDNA-TCR library based on a liquid-phase probe and application thereof, and belongs to the field of molecular immunology, and the construction and analysis method comprises the following steps: plasma cfDNA extraction, cfDNA UMI-UDI library construction, liquid-phase probe hybridization capture, enrichment of a cfDNA library containing a TCR gene, next-generation sequencing, offline data filtration and cleaning, double-end sequencing data merging, and analysis of the cfDNA-TCR library based on the liquid-phase probe. Sequencing data is compared to a reference genome, a non-specific capture sequence is removed, PCR deduplication is carried out according to UMI molecules, the data is input into TRUST4 for contig assembly and annotation, and further immune repertoire analysis is carried out according to an annotated CDR3 result. According to the method disclosed by the invention, the TCR molecules in the cfDNA can be more accurately detected by distinguishing PCR repetition from cloning repetition.

The present application relates to the field of therapeutic antibodies and molecular immunology, and specifically provides an antibody against SARS-CoV-2 coronavirus S protein and the use thereof in the preparation of a medicament for treating COVID-19. The antibody can specifically recognize and bind to SARS-CoV-2 coronavirus S protein with high affinity, which ensures that the antibody can block the infection of SARS-CoV-2 to human cells.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

This invention relates to monoclonal antibodies against CTLA4 or their antigen-binding fragments, pharmaceutical compositions, and uses. The invention belongs to the fields of tumor therapy and molecular immunology, providing monoclonal antibodies against CTLA4 or their antigen-binding fragments, pharmaceutical compositions thereof, and their uses. The monoclonal antibodies of this invention can block the binding of CTLA4 to B7, relieve the immunosuppression caused by CTLA4, and activate T lymphocytes.

This invention discloses a nucleic acid aptamer that specifically binds to the p30 protein of African swine fever (ASFV) and its applications, belonging to the field of molecular immunology. Utilizing SELEX technology, this invention screens nucleic acid aptamers from a nucleic acid library using the ASFV p30 protein as the binding antigen. Subsequently, through aptamer secondary structure prediction, aptamer affinity determination, and aptamer specificity detection, two nucleic acid aptamers with the highest binding affinity and good specificity were identified, their core sequences being shown in SEQ ID No: 1 and SEQ ID No: 2, respectively. The ASFV p30 protein-specific nucleic acid aptamer screened and prepared by this invention exhibits excellent specificity and affinity, providing important technical support for further research on ASFV antigen detection technology.

The invention belongs to the field of immunotherapy and molecular immunology, and particularly relates to a monoclonal antibody specifically binding to PD-1 or an antigen binding fragment thereof and medical application of the monoclonal antibody or the antigen binding fragment. The antibodies or antigen-binding fragments thereof are particularly useful for the prevention or treatment of cancer, inflammation and autoimmune diseases.

The invention relates to the technical field of biochemistry and molecular immunology, in particular to a mycoplasma capricolum subsp. Capricolum RS02020 protein polyclonal antibody, the polyclonal antibody is prepared by carrying out biological immunization on a recombinant protein RS02020 coded and expressed by a recombinant plasmid pET28a-RS02020, and a coding gene sequence of the recombinant plasmid pET28a-RS02020 is a nucleotide sequence shown in SEQ ID NO.1 in a sequence table. The invention relates to a preparation method of a mycoplasma capricolum subsp. Capricolum RS02020 protein polyclonal antibody. The preparation method comprises the following steps: S1, constructing a recombinant plasmid pET28a-RS02020; S2, carrying out prokaryotic expression and purification on an RS02020 protein; and S3, polyclonal antibody preparation and titer determination. Through bioinformatics analysis, subcellular localization, transmembrane helix, antigenicity and hydrophilic characteristics of the Mccp protein RS020202 are provided, soluble efficient expression is tested in escherichia coli, and high-purity RS02020 recombinant protein is obtained; the prepared polyclonal antibody is high in titer and good in specificity, and can be applied to functional research of proteins and further evaluation of CCPP antigens.

This invention belongs to the field of zoonotic disease prevention and control and molecular immunology technology, specifically relating to the application of a biological product that silences or knocks out the ID2 gene in regulating host resistance to Brucella infection. This invention constructs an ID2 gene knockout macrophage model using CRISPR / Cas9 gene editing technology and establishes a Brucella infection system. Experiments show that ID2 gene knockout significantly enhances the host's ability to clear Brucella. RNA-seq analysis shows that ID2 participates in regulating the host's transcriptional response network to Brucella, with its differentially expressed genes significantly enriched in lipid metabolism and autophagy. Mechanistic results indicate that the ID2 gene participates in the regulation of Brucella-mediated lipophage. It can be seen that the ID2 gene affects the intracellular replication level and lipophage process of Brucella. This invention provides new targets and theoretical basis for drug development against Brucella infection, immune regulation, and disease-resistant breeding.