O-type foot and mouth disease virus antigen epitope molecular mimic peptide and application thereof

A foot-and-mouth disease virus and antigen epitope technology, applied in the field of molecular immunology, to achieve good economic and social benefits, and low production costs

Inactive Publication Date: 2009-12-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, the report of efficient genetically engineered FMDV vaccine is seldom, if can screen out on the basis of FMDV neutrality epitope, develop its genetically engineered vaccine, will help to develop more effective genetically engineered vaccine

Method used

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  • O-type foot and mouth disease virus antigen epitope molecular mimic peptide and application thereof
  • O-type foot and mouth disease virus antigen epitope molecular mimic peptide and application thereof
  • O-type foot and mouth disease virus antigen epitope molecular mimic peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Screening and sequence analysis of porcine foot-and-mouth disease virus VP1 antigen mimotope

[0034] 1. Experimental materials

[0035] 1. Phage Random Peptide Library Kit

[0036] The linear 12-peptide library kit for phage surface capsid protein III display was purchased from NewEngland Biolabs, USA. The titer of the peptide library was 4×10 12 pfu / ml, random diversity 1.9×10 9 ; The genotype of the recipient strain E.coli ER2537 is F'lac q Δ(1acZ)M15proA+B+fhuA2supE thiΔ(1ac-proAB)Δ(hsdMS-mcrB)5(rk - mk - McrBC).

[0037] 2. Chemical reagents

[0038] BSA was purchased from Roche Company; IPTG and X-gal were purchased from Bebco Company; PEG8000, PEG20000, ABTS and Tween20 were purchased from Sigma Company; foot-and-mouth disease ELISA detection kit was produced by Lanzhou Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences; HRP-labeled anti-M13 monoclonal Antibodies and DEAE sephadex A50 were purchased from Pharmacia; porc...

Embodiment 2

[0071] Example 2: Application of Mimotope Peptides in Vaccines

[0072] 1. Experimental materials:

[0073] 1. Experimental animals C57BL / 6 (H-2kb) mice and Balb / c mice, 6-8 weeks old, were purchased from the Experimental Animal Center of Second Military Medical University.

[0074]2. Bacterial Strains and Genetic Engineering Vectors Host bacteria for genetic engineering: Escherichia coli DH5α, Top10′; pVAX1 vector, pBAD / gIII A vector (purchased from Invitrogen Life Science).

[0075] 3. Common reagents

[0076] Anti-HBcAg mAb was purchased from Biodesign Company, and HRP-conjugated Rabbitanti-mouse IgG was purchased from Sigma Company.

[0077] PCR primers: Synthesized by Shanghai Jikang Co., Ltd.

[0078] Commonly used enzymes: restriction endonucleases SacI, Bbe I, Nco I, Xho I, all of which are products of Takara Company. RNase is a product of Huashun Company.

[0079] Molecular biology kits: pGEM-T kit is the product of Huashun Company; PCR kit and T4 ligase kit are ...

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Abstract

The invention relates to the molecular immunology field. Although the existing foot-and-mouth disease weak poison vaccines and inactivated vaccines and other routine vaccines have good immunogenicity, but the vaccines have some unsafe factors such as reversion of virulence, incomplete virus blanching, the escape of live-virus from a preparation factory and the like so that people are motivated to find a more safe and effective FMD vaccine. In the molecular mimic peptide invention, phage display techniques are used to sieve effective FMDV epitopes to provide an O-type foot and mouth disease virus antigen epitope molecular mimic peptide and the invention also provides an application thereof in preparing immunogen and swine foot-and-mouth disease epitope peptide vaccine. The FMDV epitope peptide vaccine using the molecular mimic peptide of the invention has the antigenicity of target molecule without toxicity, is very safe and has low mass production cost and good economic effect and social effect.

Description

technical field [0001] The invention relates to the field of molecular immunology, in particular to a short peptide capable of simulating the antigenic epitope of O-type foot-and-mouth disease virus, and the application of the short peptide in preparing immunogen and porcine foot-and-mouth disease epitope vaccine. Background technique [0002] Foot and Mouth Disease (FMD) is an acute, febrile and highly contagious infectious disease of cloven-hoofed animals. The disease has many transmission routes and fast transmission speed, and it has become a pandemic in the world many times. Although the mortality rate of the disease is not high (except for young animals), it causes a decline in animal production performance, and culling animals costs a lot of manpower, material and financial resources, affects international trade, and causes heavy economic losses. For example, the FMD that broke out in my country's Taiwan in 1997, Japan and South Korea in 2000, and the United Kingdom ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K39/135A61P31/14
Inventor 孙树汉郭瀛军王越张毅周奇
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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