28 results about "Peptide library" patented technology

This invention discloses a universal detection method for tumor antigen-specific thymus-dependent lymphocytes in a broad population of East Asia, applicable to lung cancer and gastrointestinal cancers. The broad-spectrum T-cell epitope peptide library used in this detection method contains specific antigen T-cell epitope peptides presented by 13 HLA-A molecules, 15 HLA-B molecules, and 14 HLA-C molecules with an allele frequency greater than 1% in the East Asian population. The total gene frequencies of these dominant HLA-A, B, and C molecules account for approximately 95%, 71%, and 93% of the Chinese population, respectively, and are similar in total gene frequencies in Northeast and Southeast Asian populations. Therefore, this detection method is applicable to the vast majority of individuals in this region, providing a universal method for evaluating specific tumor antigen-specific T-cell immune function in patients with lung cancer or gastrointestinal tumors.

The application discloses a method for determining key amino acid residues of bone collagen peptide promoting osteogenesis activity, belongs to the field of biological medicine and food science, and aims to solve the technical problem that traditional methods are low in efficiency, insufficient in accuracy, and cannot efficiently identify key amino acid residues of bone collagen peptide determining the activity of promoting osteogenesis. The application comprises the following steps: firstly, obtaining target bone collagen peptide and constructing a single-point alanine mutant peptide library thereof; then, sequentially performing conformation and physicochemical property characteristic analysis, binding free energy simulation calculation with specific receptor protein, and cell experiment determination of the activity of promoting osteogenesis on the target peptide and the mutant peptide; finally, screening the key amino acid residues based on multi-level joint determination of characteristic deviation, binding free energy change and activity decline ratio. The application is mainly used for quickly and accurately positioning the core functional site of bone collagen peptide promoting osteogenesis activity, and provides key theory and technical basis for efficient screening, rational design and function strengthening of osteogenic activity peptide.

The invention aims to provide a peptide for accelerating fat metabolism and application thereof, the peptide is prepared into a product convenient to use, and the amino acid sequence of the peptide is MVHSYSETSD. The peptide is obtained through peptide library screening, bioinformatics analysis screening and fat cell decomposition experiment verification, and it is verified that the active peptide obtained through screening has the effects of accelerating fat metabolism, inhibiting fat accumulation, reducing blood fat and body fat content and reducing body weight; the insulin sensitivity and the glucose tolerance are improved, and the prevention and treatment effects on obesity and complications thereof are realized.

The application aims to provide a peptide for accelerating fat metabolism and application thereof, and to prepare the peptide into a product convenient to use, and the amino acid sequence of the peptide is MVHSYSETSD. The peptide is obtained through screening of a peptide library, screening through bioinformatics analysis, and verification through fat cell decomposition experiment, and the application verifies that the active peptide screened has the effect of accelerating fat metabolism, inhibiting fat accumulation, reducing blood fat and body fat content, reducing body weight, improving insulin sensitivity and glucose tolerance, and realizing the prevention and treatment effect on obesity and its complications.

This invention proposes a method for screening bisphenol A-specific peptides using molecular simulation, belonging to the field of food safety technology. The method includes the following steps: 1) Through protein stacking and molecular docking, the molecular structure and interactions of bisphenol A-protein cocrystal compounds in a protein structure database are comprehensively analyzed to obtain the receptor protein and the original parent chain; 2) Based on the original parent chain obtained in step 1), the parent peptide is obtained by truncation of the parent peptide segment by analyzing the main active amino acids in the molecular docking results; 3) By performing virtual amino acid mutations on the parent peptide obtained in step 2), a peptide library specifically binding to bisphenol A is constructed, and molecular dynamics is used for preliminary screening of the peptides; 4) The peptide chains screened in step 3) are used as specific recognition elements, and peptides labeled with fluorescein isothiocyanate are used to form specific recognition probes. The specific recognition probes are mixed with bisphenol A, and the peptide sequences with the strongest specific binding ability to bisphenol A are screened based on the different fluorescence differences. This invention is mainly applied to the screening of specific recognition elements for toxic and harmful small molecules, active ingredients, and functional factors. This method avoids the complex preparation steps of antibodies and the cumbersome screening process of aptamers, achieving high-throughput design of specific recognition elements.

The present disclosure provides a peptide library comprising stable isotope-labeled (SIL) peptides, wherein the peptides comprise or consist of the sequences according to Table A, Table B, or Table C.

Disclosed is a fluorescently labeled peptide library useful for diagnosing multiple sclerosis. The fluorescently labeled peptide library comprising fluorescently labeled peptides represented by the following formula:wherein X1, X2, X3 and X4 are independently arbitrary amino acid residues, and the peptides are immobilized on a chip.

The invention relates to the technical field of biology, in particular to affinity polypeptide of human serum albumin and a design method of the affinity polypeptide. The molecular mechanism and microscopic details of interaction of HSA-Mgbc7HopQNbAlb1 compounds are analyzed through an MD simulation technology and an MM-PBSA free energy decomposition method, key binding sites between the compounds and spatial distribution of the key binding sites are determined, and an affinity model is established, so that a candidate affinity polypeptide library is constructed, and meanwhile, the peptide library is expanded through intelligent design. Then, screening and verifying the affinity polypeptide library through methods of molecular docking, conformation analysis, hydrophobicity analysis, MD simulation and the like, so as to obtain the high-affinity polypeptide aiming at the HSA target spot. The binding performance of the affinity polypeptide and the HSA is verified through a double-antibody one-step sandwich ELISA experiment, adsorption isotherm determination and an affinity chromatography experiment. The polypeptides such as FKITSGSLSR, DGALTPPSEY and the like obtained by screening have high affinity with the HSA (Human Serum Albumin).

The invention relates to the technical field of biology, in particular to a p53 protein targeting inhibitor and a design method thereof. The molecular mechanism and microscopic details of interaction of azurin-p53 protein complexes are analyzed through an MD simulation technology and an MM-PBSA free energy decomposition method, key binding sites between the complexes and spatial distribution of the key binding sites are determined, and an affinity model is established, so that a candidate affinity ligand peptide library is constructed. Then, screening and verifying the affinity ligand library through methods of molecular docking, conformation analysis, binding site comparison, hydrophobicity analysis, MD simulation and the like to obtain a high-affinity ligand aiming at a p53 protein target spot, and verifying the binding performance of the affinity peptide ligand and the p53 protein through a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) experiment. And screening to obtain the p53 protein inhibitory peptide with high affinity with the p53 protein.

This invention discloses a method for de novo design of antifungal peptides, the obtained peptides, and their applications. The method includes: S1 peptide library retrieval and amino acid preference analysis; S2 peptide design based on multiple parameters; S3 AI high-throughput screening of the peptide library to obtain candidate antifungal peptides. The amino acid sequences of the mentioned antifungal peptides are also disclosed in this invention as shown in SEQ ID Nos: 1 to 3. The antifungal peptides developed by this invention can specifically target and kill fungi such as Malassezia furfur without inhibiting the growth of other beneficial bacteria such as Propionibacterium acnes, thereby effectively regulating the skin surface microecological balance and maintaining skin health; solving the problem that current antimicrobial peptides, due to their broad-spectrum antibacterial properties, can disrupt the skin microecological balance and cause more serious skin diseases.

The application relates to a method for generating a target AAV9 antibody epitope affinity peptide ligand by using a protein language model and a screening method and application thereof, and relates to an affinity peptide candidate sequence construction and screening method, an affinity peptide ligand, an affinity medium and application thereof for targeting adeno-associated virus type 9 (AAV9). The AAV9 capsid region recognized by Fab2-4 is taken as a target, structure analysis, natural amino acid positioning, key residue extraction and affinity peptide template construction are carried out, a candidate peptide library is generated by using a protein language model, and a candidate affinity peptide ligand is screened by combining molecular docking, binding free energy evaluation, refined docking and molecular dynamics simulation. The affinity peptide ligand is any one of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4, can be immobilized on a solid phase matrix to form an affinity medium, is used for AAV9 recognition, capture and purification, and provides a technical scheme for AAV9 purification process development.

The invention provides a litopenaeus vannamei head umami peptide and a preparation method thereof, and the umami peptide is characterized in that the amino acid sequence of the umami peptide is selected from any one of the sequences as shown in SEQ ID NO: 1-5; the delicate flavor threshold value of the delicate flavor peptide is 0.2 to 0.5 mmol / L. According to the method, high-value utilization of the penaeus vannamei head byproducts is achieved, and the problems of wasting of aquatic product processing resources and environmental protection pain are effectively solved; five brand-new umami peptide sequences are obtained for the first time, the umami threshold value is far lower than that of monosodium glutamate, the synergistic fresh-increasing effect is outstanding, and the blank of an aquatic product source umami peptide library is filled.

The present technology provides an approach to designing libraries of peptide sequences for discovery and testing of significantly more motifs than would be otherwise available in a given fixed library format. The technology includes a plurality of x-mers embedded in N-mer peptides sequences, where N and x are integers and where N is greater than x. This approach provides for the representation of multiple unique x-mer peptides in a single N-mer peptide feature.

The invention relates to the technical field of biology, and discloses an antioxidant peptide derived from fermented meat and application of the antioxidant peptide, the antioxidant peptide comprises minced pork, auxiliary materials and a composite leavening agent composition composed of lactobacillus sake and staphylococcus xylosus, and fermentation and maturation are carried out by controlling the inoculum size and adopting a two-stage temperature control process. The core innovation of the method is as follows: firstly, performing comprehensive peptide sequence identification on small molecular weight components extracted from a fermentation product by adopting liquid chromatography-tandem mass spectrometry, and then accurately predicting and locking a target antioxidant peptide from a peptide library by utilizing virtual screening technologies such as molecular docking and the like. The antioxidant peptide prepared by the invention can be used for effectively relieving cell oxidative damage induced by ethanol by activating a Kelch sample ECH associated protein 1-Nrf2 (Keap1-Nrf2) signal channel. According to the method, directional fermentation and computational biology are combined, a new normal form is provided for efficiently discovering novel active peptides from natural products, and the research and development efficiency is effectively improved.

A method for co-compartmentalising a cyclic polypeptide with a polynucleotide encoding the cyclic polypeptide, comprising the steps of a) forming a compartment containing a polynucleotide encoding the cyclic polypeptide, b) expressing a polypeptide from the polynucleotide, and c) cyclising the polypeptide. Co-compartmentalised cyclic polypeptides and encoding polynucleotides. Libraries of co-compartmentalised cyclic polypeptide and encoding polynucleotide. Methods for screening libraries of co-compartmentalised cyclic polypeptide and encoding polynucleotide. Incorporation of non-canonical nucleic acids into such libraries.

A method for constructing a DNA-encoded library, specifically, (s1) providing an HP module, the HP module comprising a nucleic acid; (s2) covalently attaching chemical structural units to the HP module to form chemical moieties attached to the HP module, while covalently attaching oligonucleotides that encode the chemical structural units to the HP module to form encoded nucleic acid moieties of the HP module, thereby forming a nucleic acid-compound conjugate; (s3) subjecting the nucleic acid-compound conjugate to a linking reaction with a compound shown in formula (II) to obtain a first mixture; S-R5 (II), wherein the S is a solid-phase carrier, and R5 is a second group participating in the linking reaction; (s4) subjecting the first mixture to filtration, washing, and purification, and then to cleavage to obtain a new compound; and (s5) repeating steps (s2)-(s4) x times, wherein x is an integer greater than 0, thereby forming a DNA-encoded library. The obtained DNA-encoded library has a larger load capacity, high purity, a larger peptide library, and wider applicable reactions.

The invention provides a specific monoclonal antibody for resisting pseudorabies virus IE180 protein as well as a preparation method and application of the specific monoclonal antibody, and belongs to the technical field of biological medicines. The invention provides a hybridoma cell strain pIE180 mAb for producing a specific monoclonal antibody against the pseudorabies virus IE180 protein, and successfully prepares the monoclonal antibody against the PRV IE180 protein. An overlapping peptide library technology shows that the monoclonal antibody can recognize a new B cell epitope, and the epitope is highly conservative in various classic and mutant isolates, can be used for specific detection of PRV infection and development of diagnostic reagents, provides an important molecular basis for design of subunit vaccines, and has broad application prospects. Important clinical application and market prospects are realized.

Methods and systems are provided herein for selecting an affinity reagent which binds a desired peptide epitope in a plurality of sequence contexts. The method relies on obtaining a peptide library, each peptide having the sequence αXβ, wherein X is the desired peptide epitope, wherein each of α and β comprise an amino acid, using the peptide library to select an affinity reagent.

The present invention relates to methods of producing a conformationally constrained peptide in cellulo using a pnictogen-containing crosslinker. The present invention also relates to conformationally constrained peptides obtainable by said methods and a method for screening peptide libraries for transcription factor agonists that tests whether said conformationally constrained peptide inhibits association between two candidate binding partners. The present invention also relates to the use of a pnictogen-containing cross-linker in a method of producing a conformationally constrained peptide in cellulo. The present invention also relates to recombinant peptides comprising three thiol- / selenol-containing residues linked to a pnictogen-containing cross-linker. The present invention also relates to a kit for use in said methods.

Parkinson's disease (PD) is a neurodegenerative disorder that causes weakness in humans, characterized by the accumulation of alpha-synuclein aggregates in the brain. Development of effective therapies targeting alpha-synuclein is challenging due to the inherent disorder properties of alpha-synuclein. Peptide inhibitors or alpha-synuclein binding peptides (alpha-SBP) that bind alpha-synuclein with nanomolar affinity and are effective in inhibiting aggregation thereof are disclosed. Peptide libraries are constructed using phage displays in which peptide inhibitors, such as PD-6, appear as nanomolar binding agents, can enter neuronal cells and bind to alpha-synuclein. In general, alpha-SBP, such as PD-6, has been demonstrated to inhibit the formation of alpha-synuclein aggregates in vitro and in vivo, and exhibit a therapeutic effect in a subject.

The application belongs to the field of biochemistry, and discloses a tyrosine-based polypeptide modular post-modification method and application thereof. The polypeptide modular post-modification method is as follows: adding a polypeptide, 5-bromo-1,2,3-triazine and an inorganic base in a reaction solvent, stirring and reacting, then adding an electron-rich dienophile, stirring and reacting at a certain temperature, and then concentrating, separating and purifying the mixture to obtain a polypeptide modular post-modification product. The polypeptide modular post-modification method has the advantages of simple raw material synthesis, simple operation, mild reaction conditions, high reaction efficiency, wide substrate application range, and compatibility with liquid phase and solid phase synthesis. In addition, the polypeptide modular post-modification method can be applied to constructing a dual-modular peptide imaging probe, realizing long-acting modification of endogenous polypeptides, and constructing a new type of antibacterial short peptide library, and provides a new and feasible method for the development of polypeptide drugs.

The invention discloses a method for directionally enriching and screening peptide fragments capable of effectively promoting the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) from complex biological enzymatic hydrolysate, and belongs to the field of biotechnology and active peptide screening. The method comprises the following steps: co-immobilizing ADH1B and ALDH2 on the same solid-phase carrier in a high-activity manner by adopting a segmented pH sequential coupling technology to prepare a dual-enzyme affinity carrier; the carrier is used for adsorbing peptide fragments capable of being specifically combined with double enzymes in a complex peptide library; step-by-step elution is carried out by utilizing a specific competitor of ADH1B and ALDH2, so that two types of targeted peptide enriched components are respectively obtained; the specificity is verified through reverse screening and alcohol metabolism enzyme activity promotion function determination. According to the invention, high-efficiency parallel screening and synergistic combination discovery of alcohol metabolism key pathway double-target active peptides are realized for the first time, and an innovative technical platform is provided for developing a multi-target and high-efficiency alcohol metabolism regulator.

The application belongs to the technical field of polypeptide cyclization, and specifically provides a cyclic peptide construction method based on thiol-alkene photo-click chemistry, comprising the following steps: S1, designing a polypeptide sequence containing multiple cysteines; S2, reducing disulfide bonds in the polypeptide through a thiol reducing agent; S3, under ultraviolet light irradiation, performing thiol-alkene photo-click chemistry reaction on the reduced polypeptide, a crosslinking agent and a photoinitiator to form a cyclic peptide structure. The method uses ultraviolet light to excite the addition reaction of thiol and alkene, generates stable thioether bonds, constructs a cyclic peptide structure, has high selectivity and high efficiency, and is performed under mild conditions without using a metal catalyst. The method is applied to the construction of a phage display cyclic peptide library, and has wide application potential in the construction of a bicyclic peptide library. The obtained cyclic peptide has significantly enhanced stability, and provides an effective tool for screening high-quality cyclic peptide ligands that specifically bind to a target.

This invention discloses a tetanus vaccine and its enzyme-linked immunosorbent assay (ELISpot) analysis method. The tetanus vaccine of this invention is designed based on screened tetanus antigen peptides, which contain any of the amino acid sequences shown in SEQ ID NO:1-SEQ ID NO:61. Addressing the technical problem of the lack of specific T-cell immune response detection methods for existing tetanus vaccines, this invention designs an overlapping peptide library covering the entire sequence of tetanus toxoid, screening out dominant peptides that can effectively stimulate T-cell responses, significantly improving the sensitivity and specificity of detection. This method optimizes the processing flow of PBMCs in the test samples, establishes a quantitative analysis standard based on IFN-γ secretion, and achieves accurate assessment of the T-cell immune response induced by the tetanus vaccine.

The application discloses umami peptides derived from Penaeus monodon and application thereof, and belongs to the technical field of bioactive peptides. The umami peptides are 11 in number, and the amino acid sequences are respectively ACWVPCEK, QRMMQ, DAKKACW, DRM, RMM, PDPDPT and the like, as shown in SEQ ID NO. 1-11. The umami peptides are applied in umami agents. The umami peptides are obtained from the myoglobin of Penaeus monodon by using a rapid screening technology, wherein the umami intensity of QRMMQ is the strongest, the umami threshold of PDPDPT is the lowest, and ACWVPCEK has a synergistic umami effect. The application enriches the umami peptide library of marine origin, improves the economic value of Penaeus monodon, and simultaneously studies key amino acids and an action mechanism of umami peptide binding with umami receptors, thereby providing a reference for studying the umami mechanism of umami peptides.

The invention belongs to the technical field of cosmetics, and particularly relates to a cyclic peptide with functions of promoting collagen synthesis, hair follicle activation and scalp aging resistance and application of the cyclic peptide in cosmetics. According to the invention, a novel cyclic peptide capable of regulating and controlling hair follicle activation and scalp anti-aging related signal channels is found through peptide library screening, and a structurally-similar peptide of the cyclic peptide is synthesized, and the structure of the cyclic peptide is shown as a formula (I) or (II). The cyclic peptide can effectively up-regulate the expression of hair follicle activation marker genes, promote the proliferation of hair follicle dermal papilla cells and enhance the antioxidant capacity, and can also promote the synthesis of collagen, so that the cyclic peptide can be used for preparing cosmetic compositions for preventing and treating alopecia, promoting hair growth and resisting scalp aging. (I) (II)

The invention relates to the technical field of preparation of plant growth regulators, in particular to a growth regulator containing plant antibacterial peptides. Comprising the following steps: preparing a plant antibacterial peptide solution, preparing an antibacterial peptide-loaded bio-based wet film, preparing an antibacterial peptide-bacterial cellulose nano suspension, preparing an SA-AMPS auxiliary agent, and preparing the growth regulator containing the plant antibacterial peptide. The degreased broad bean powder and the degreased Chinese prickly ash seed meal are subjected to common enzymolysis to prepare the plant antibacterial peptide solution, and the degreased broad bean powder and the degreased Chinese prickly ash seed meal are different in amino acid composition, sequence and protein structure and can provide more diverse cleavage sites for protease as enzymolysis substrates together; the amino acid sequence and the space structure of the compound antibacterial peptide library are diversified, the variety and the number of active peptide fragments in the product are increased, the compound peptide library generated by synergistic enzymolysis of the amino acid sequence and the space structure can initiate multi-target and multi-mechanism synergistic attack on ralstonia solanacearum, the antibacterial spectrum is expanded, and the antibacterial rate is remarkably increased.

This invention belongs to the fields of genetic engineering and protein engineering technology, specifically relating to a norovirus-specific CD8. + T-cell epitope peptides and their applications. This invention focuses on the CD8 specific capsid protein of the NV virus. + The study focused on T cells, using in vitro detection methods to sequentially screen a peptide library and peptide fragments that exhibit dominant immune responses to capsid proteins. Subsequently, based on these dominant immune peptides, a CD8-specific antibody against NV immune dominance was constructed. + The in vitro expansion and culture system for T cells successfully cultured VP1-P23, VP2-P16, and VP2-P26 dominant peptide-specific CD8. + T cells. Furthermore, based on the dominant 18 peptides, epitopes of the core short peptides were sequentially identified. This invention relates to norovirus-specific CD8... + T-cell epitope peptides can be used for the diagnosis of NV infection, and the superior response in previously infected individuals has multiple protective effects, which can be used for the development of antiviral vaccines.