298 results about "Bioactive peptide" patented technology

The invention discloses a blood protein precise active peptide capable of tonifying qi and blood, improving microcirculation and promoting vasodilation and a preparation method and application thereof, and belongs to the technical field of blood protein peptide. The blood protein precision active peptide comprises at least one of LGFP, IGFP, FPHFN, LFL, LFI, FESF, ILF, LIF, FLL, FIL, FII or FPHFD. The prepared blood protein precision active peptide has multiple biological activities: an antihypertensive effect is achieved by inhibiting ACE and vasoconstriction substances; vasodilation is promoted by activating a PI3K / AKT / eNOS signal channel; a zebra fish model proves that the traditional Chinese medicine composition has the effects of remarkably improving microcirculation and tonifying qi and blood. The preparation method is low in equipment requirement, simple in process, easy to operate and convenient for industrial large-scale production. The method enriches a bioactive peptide library, and is of great significance in promoting technical progress and industrial upgrading in the field of hemoglobulin peptides.

The invention particularly relates to a ginseng peptide with anti-aging activity and a preparation method and application thereof, and belongs to the technical field of bioactive peptides. The method comprises the following steps: by taking ginseng fibrous roots, ginseng leaves and other by-products as raw materials, carrying out weak acid ultrasonic pretreatment, loading natural anti-aging active components, carrying out low-temperature high-pressure homogenization and airflow crushing synergistic treatment, carrying out double-stage composite enzymolysis, clarifying with an alternating electric field ceramic membrane, carrying out five-stage series ultrafiltration classification, carrying out four-stage filter element sterilization, freeze-drying and the like in sequence. The high-purity ginseng peptide is prepared. The total nitrogen content of the prepared ginseng peptide is larger than or equal to 14.8%, the proportion of a target peptide fragment is larger than or equal to 97.2%, the yield is larger than or equal to 18.5%, and the ginseng peptide has good antioxidant and anti-aging activity and can be used for development of functional food and skin care products. The method is low in cost, high in resource utilization rate and green and efficient in process, and the product purity and activity are remarkably superior to those of a traditional method.

Provided herein are peptide amphiphiles (PAs) comprising a bioactive peptide, nanofibers displaying the bioactive PAs, and methods of use thereof. The disclosed peptide amphiphiles comprise a hydrophobic tail, a structural peptide segment, a charged peptide segment, and a bioactive IKVAV peptide. The disclosed PAs may be used in cell culture methods and in methods of treating central nervous system injury.

The invention discloses a scallop skirt ACE inhibitory peptide activity method and system based on deep learning, and relates to the technical field of bioengineering.The scallop skirt ACE inhibitory peptide activity method comprises the steps that a comprehensive data set is obtained based on simulated in-vivo test data and peptide level feature data of different peptides, key features are screened, and a training set and a test set are divided; initializing a random forest model, an SVM model and a logistic regression model, performing independent training by using the training set, and determining an optimal prediction model of the peptide antihypertensive activity based on a prediction result of the test set; extracting importance data of key features based on the optimal prediction model for the antihypertensive activity of the peptide, sorting the importance data, and visually analyzing the antihypertensive activity features, feature importance sorting and model performance of different peptides; and all key results in the analysis process are integrated and systematically stored. According to the method, normal form reference with prediction accuracy and mechanism depth can be provided for clinical transformation of marine bioactive peptides, and AI-driven novel peptide drug research and development are promoted to enter a new stage.

The invention belongs to the technical field of bioactive peptides, and discloses a white snakehead oligopeptide as well as a preparation method and application thereof. The white snakehead oligopeptide is selected from one of oligopeptides with amino acid sequences as shown in SEQ ID NO.1-8. The invention further discloses a preparation method of the white snakehead oligopeptide. The polypeptide provided by the invention has a relatively high ferrous / zinc absorption rate to a human body, has a certain function of treating iron-deficiency anemia and zinc deficiency, and has a good application prospect in mineral (iron / zinc) supplements, functional foods and health-care products.

Use of an effective amount of the bioactive peptide in the preparation of a medicament for the prevention and / or treatment of hypoxic-ischemic encephalopathy in newborns, wherein the amino acid sequence of the bioactive peptide contains KKNRNKLRRQHSY or a functionally equivalent variant thereof. Optionally, the bioactive peptide further comprises a signal peptide at its N-terminus or C-terminus.

The invention discloses a preparation process of ready to dye ready to dye and easy to dye easy to dye hair, and particularly relates to the technical field of hair treatment chemistry, the process is realized through three stages of pretreatment, modification treatment and neutralizing and shaping which are sequentially executed, the hair is immersed in a solution containing an alkaline reagent system and a bioactive peptide structure enhancer, and then the hair is soaked in a solution containing an alkaline reagent system and a bioactive peptide structure enhancer; at a specific temperature, synchronously opening hair cuticles and repairing keratin disulfide bond damage; under the catalysis of a rare earth metal complex catalyst, a permanent cationization three-dimensional network is formed through covalent grafting of an amphiphilic quaternary ammonium salt compound and a keratin nucleophilic site; a neutralizing solution containing an organic acid system and reactive siloxane is adopted to close hair cuticles, an ionic bond anchored hydrophobic protective film is formed, a biological peptide synergistic repair mechanism effectively overcomes keratin damage caused by traditional alkali treatment, and the completeness of the hair cuticles is remarkably improved; a high-density permanent cation site is constructed through rare earth catalytic covalent grafting, so that the dye adsorption strength is improved, and the problem that hair dyeing process damage and durability are difficult to consider at the same time is solved.

This invention discloses an anti-inflammatory peptide derived from pearl oysters and its applications, relating to the field of bioactive peptide technology. The amino acid sequence of this anti-inflammatory peptide is TAMY. The anti-inflammatory peptide derived from pearl oysters provided by this invention can regulate the release of cellular inflammatory mediators, reduce the levels of NO and pro-inflammatory cytokines (such as TNF-α, IL-6, and IL-1β), and simultaneously promote the secretion of anti-inflammatory cytokines (such as IL-10), exhibiting excellent anti-inflammatory activity and suitable for use in functional foods, cosmetics, or pharmaceutical ingredients.

The invention belongs to the technical field of bioactive peptides, and particularly relates to a phycocyanin peptide and application thereof in preparation of a product with functions of inhibiting PTGS2 expression and regulating an Akt / GSK3beta signal channel. According to the invention, phycocyanin is taken as a raw material, active polypeptides with specific sequences are obtained through an enzymolysis separation technology, and the amino acid sequences of the active polypeptides are respectively Leuu-Ala-Ser and Leuu-Gly-Leu. Experiments prove that the phycocyanin peptide prepared by the invention can significantly inhibit the expression of PTGS2, and can play a related regulation role by activating an Akt / GSK3beta signal channel, so that the technical blank of plant active peptide targeting PTGS2 and Akt / GSK3beta signal channels is filled, and the phycocyanin peptide can be widely applied to the development of related products for regulating the abnormal expression of PTGS2 and Akt / GSK3beta signal channels.

The present disclosure provides a nanobody platform having a high affinity purification on protein A, wherein the nanobodies may incorporate diverse bioactive peptides, lipid or glycoside in its CDR1 and / or CDR3 regions. The present disclosure also provides a peptide-modified nanobody comprising non-toxic bioactive peptides and that exhibit potent anti-tumor activity. Moreover, the peptide-modified nanobody is able to be combined with other technologies, such as but not limited to bispecific antibodies and antibody-drug conjugates. Further, the present disclosure also provides production processes of the nanobodies and their use as a treatment and diagnostic agents.

Described herein are isolated peptides, compositions comprising the same, and methods of using such peptides or compositions in the treatment of depression, central nervous system disorders, and neurodevelopmental disorders.

The invention belongs to the technical field of bioactive peptide preparation and food processing, and discloses an ultrasonic-assisted preparation method of giant salamander muscle peptide zinc chelate. The preparation method comprises the following steps: pretreating giant salamander muscles; preparing giant salamander muscle polypeptide; preparing a chelate product of giant salamander muscle peptide and zinc sulfate heptahydrate under an ultrasonic condition; and carrying out alcohol precipitation separation on a chelation reaction product. The giant salamander muscle peptide zinc chelate prepared by the invention can be directly used as a natural nutrition enhancer to timely supplement nutritional ingredients necessary for a human body. The preparation method provided by the invention has the advantages of short production cycle and no pollution to the environment, and the obtained product has stable performance and high absorption efficiency and can be widely applied to the fields of foods, medicines and health care.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

The invention discloses a preparation method of collagen micromolecule active peptide and bioactive peptide for inhibiting cancer cell proliferation and improving intracellular environment, and belongs to the technical field of bioactive peptide preparation and biological functional raw materials. According to the method, bone collagen peptide, cartilage peptide (hydrolyzed II-type collagen peptide), collagen peptide (German Jilida), non-denatured II-type collagen peptide and yak bone marrow peptide are taken as core raw materials, and optional ingredients such as bovine bone marrow peptide powder, shark cartilage peptide and Belgian imported inulin can be compounded as required; through a step-by-step preparation process comprising the steps of graded purification pretreatment of raw materials, precise proportioning and mixing, low-temperature activation and synergism and stable micro-capsule embedding, structure protection and efficacy synergy of each collagen small-molecule active peptide and bioactive components are realized; the prepared bioactive peptide product can significantly inhibit cancer cell proliferation, regulate intracellular oxidation-reduction balance and improve intracellular microenvironment, the preparation process is mild and free of high-temperature damage, the raw material biological activity retention rate is high, the raw material source is controllable, the large-scale production feasibility is high, and the bioactive peptide product can be widely applied to preparation in the fields of functional food, bioactive raw materials and the like.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

The invention provides a peptide liquid enrichment and hydroponic irrigation intelligent fish and vegetable peptide integration method, which comprises the following steps: S1, obtaining a preset production strategy selected by a user through an input device, and obtaining an operation mode selected by the user; s2, according to the operation mode, adopting a multi-target collaborative optimization algorithm to generate operation instructions of a culture unit, a water culture unit and a peptide extraction unit; s3, sending the operation instruction to the culture unit, the water culture unit and the peptide extraction unit through a standardized interface, and obtaining the operation state of each unit; s4, adjusting a water flow parameter of the culture unit according to the running state, and generating a first water body state; s5, extracting bioactive peptides from the first water body state to generate a second water body state; s6, adjusting nutrient solution supply parameters according to the second water body state, and generating a plant growth state; s7, the treated third water body state flows back to the culture unit, culture parameters are adjusted, and the collaborative result of fish growth and water quality optimization is achieved.

This invention discloses a guanylate cyclase-C (GC-C) activating peptide and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the GC-C activating peptide is PFPGPIPN. The GC-C activating peptide of this invention can significantly activate GC-C activity, increasing intracellular cGMP levels. Furthermore, the GC-C activating peptide of this invention can alleviate constipation and abdominal pain symptoms in mice, thereby effectively preventing or improving irritable bowel syndrome (IBS). Therefore, the GC-C activating peptide provided by this invention has advantages such as high activity, safety and non-toxicity, and good water solubility. It can be used as a candidate molecule for alternative or adjuvant drugs in the clinical treatment of IBS, and also as a health product or food to improve constipation or abdominal pain.

The application belongs to the technical field of biotechnology and particularly relates to a multifunctional bioactive peptide based on highland barley wine lees protein and a preparation method thereof, wherein the amino acid sequence of the bioactive peptide is shown as SEQ ID NO:1. The bioactive peptide has small molecular weight, is easy to absorb, can inhibit tyrosinase activity and efficiently scavenge free radicals, and has the advantages of non-toxicity, non-carcinogenicity, high hydrophilicity, long half-life and the like through bioinformatics analysis prediction, and can be applied to the development of drugs for treating skin pigmentation and oxidative stress related diseases or daily chemicals for whitening, anti-aging, antioxidant and the like, and has a wide application prospect.

This invention relates to the field of bioactive peptide preparation technology, and discloses a method for preparing and applying a bifunctional antioxidant and anti-inflammatory peptide based on porcine skin collagen. The bifunctional peptide has the amino acid sequence GPSGPPGEKGP. Through peptidomics and computer simulation screening, this invention identified five novel antioxidant peptides with good solubility, non-sensitizing properties, and non-toxicity. Using an AFB1-induced HuH7 cell oxidative damage model, in in vitro cell experiments, GP-11 was shown to significantly reduce ALT, AST activity, and ROS levels, enhance the activity of SOD, CAT, and GSH-Px antioxidant enzymes, and activate the Keap1-Nrf2 pathway. It also reduced the levels of inflammatory factors TNF-α and IL-6 by downregulating the classical cGAS-STING inflammatory pathway. GP-11 exhibits both significant antioxidant effects and the ability to reduce the levels of inflammatory factors, demonstrating a dual function of antioxidation and anti-inflammation.

The invention discloses an oyster-derived polypeptide and application thereof, and belongs to the technical field of bioactive peptides. The oyster source polypeptide provided by the invention is selected from at least one of the following polypeptide sequences: PAPLWQMPKFRN, NPVDPAPLWQMPK, PFPL, VPPF, PLNF, APAPAVKYLR, PPMIFKTR, PPMIFKTRIM, AVNMVPFPR, FSLP, PFKP, FPSIVGRPR, VPKPYLNHPL, LPPL, PQDVPPKKPLIMIAAR, VPFVPYLNHPL, FLLK, SPPELPDVM and MPVTDPPLRTF. The invention also discloses a preparation method of the oyster source polypeptide. The invention also discloses application of the oyster-derived polypeptide in preparation of food, health care products or medicines with an immune regulation function. The oyster source peptide product disclosed by the invention has digestion stability and immunocompetence, can keep a basic structure after simulating gastric juice digestion, and can activate the immune function of RAW264.7 cells and improve the expression quantity of cell factors. The method provided by the invention can provide a new direction for high-value utilization of marine resources, and has a good application prospect.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

The application discloses a quinoa hexapeptide with a blood sugar lowering effect and application thereof, and belongs to the technical field of bioactive peptides. The amino acid sequence of the quinoa hexapeptide PGSPGR is Pro-Gly-Ser-Pro-Gly-Arg. The quinoa hexapeptide PGSPGR has alpha-glucosidase inhibitory activity and can effectively reduce blood sugar level, and therefore, can be applied to preparation of medicines or food for lowering blood sugar. The application further provides a polypeptide containing the peptide segment PGSPGR and a quinoa peptide rich in the polypeptide, and the polypeptide and the quinoa peptide are applied to preparation of medicines or food for lowering blood sugar, thereby providing a new scheme and theoretical basis for treatment of diabetes, and having good market prospect and application potential.

The application discloses corn active peptides with effects of dispelling alcohol, reducing uric acid and resisting oxidation, a preparation and application thereof, and belongs to the technical field of bioactive peptides.The amino acid sequence of the corn active peptides is at least one of LMFP, FEGLFR, FLR and QLPSYR.The corn active peptides with effects of dispelling alcohol, reducing uric acid and resisting oxidation are separated from corn germ meal through enzymolysis, ultrafiltration and other technologies, the amino acid sequences of the separated peptide segments are determined, and bioinformatics technology is combined for screening, and finally four peptide segments are obtained.The identified peptide segment sequences are chemically synthesized, and the effects of dispelling alcohol, reducing uric acid and resisting oxidation of the synthesized single peptide segments and compound peptide segments are determined.The corn active peptides all have good XOD inhibition rate, DPPH clearance rate and ADH activation rate, and have important application prospects in the development of products with effects of dispelling alcohol, reducing uric acid and resisting oxidation.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The application discloses a polypeptide with uric acid reducing activity and a preparation method and application thereof, relates to the field of bioactive peptides, and provides a preparation method of the polypeptide with uric acid reducing activity, which takes whey protein as raw material, and obtains the polypeptide with high activity through directional enzymolysis and ultrafiltration purification, and the polypeptide can reduce blood uric acid level by inhibiting xanthine oxidase activity, and the method has the advantages of high efficiency, low cost and easy implementation, can be implemented on a large scale, and is helpful to improving the utilization value of whey protein in the industry, and provides a new way for the development of functional natural anti-uric acid level abnormality or xanthine oxidase activity abnormality related disease products.

The present application provides a kind of bioactive seventeen peptides and its application, belong to bioactive peptide technical field, the amino acid sequence of the seventeen peptides is as shown in SEQ ID NO.1;Seventeen peptides provided by the present application can significantly reduce immune damage, improve immune organ index, reduce spleen tissue damage, can significantly promote the secretion level of immunoglobulin and cytokine in blood;The polypeptide has immunoregulation, can improve immunity, can be used for preparing the product of enhancing immunity.

This invention discloses an octapeptide derived from soybean protein with the function of lowering blood pressure, blood sugar, and cholesterol, as well as its preparation and application, belonging to the field of small molecule peptide technology. The octapeptide sequence is YEGNWGPL, which can be obtained artificially or by targeted enzymatic hydrolysis of soybean protein isolate. Functional verification in a hyperlipidemia model shows that the octapeptide YEGNWGPL has the function of lowering blood lipids; functional verification in a hyperglycemia model shows that the octapeptide YEGNWGPL has the function of lowering blood sugar, with a significantly better effect than the drug metformin; functional verification in a hypertension model shows that the octapeptide YEGNWGPL has the function of lowering blood pressure, with a significantly better effect than the antihypertensive drug captopril; moreover, food-derived bioactive peptides have high biosafety, therefore, they can be applied to the development of related products for lowering blood pressure, blood sugar, and cholesterol or assisting in lowering blood pressure, blood sugar, and cholesterol, with good market prospects and application potential.

The application discloses selenium-containing chickpea peptides, a preparation method thereof and application of the selenium-containing chickpea peptides in preparation of blood pressure lowering drugs, and belongs to the technical field of bioactive peptides. Selenium-enriched Bacillus natto is used to ferment chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then fermentation conditions are optimized and quantitative peptidomics technology is used to obtain selenium-containing chickpea peptides PQM and PQML. Experiments prove that the selenium-containing chickpea peptides have good in-vivo stability, bioavailability and blood pressure lowering activity, have an effect of inhibiting angiotensin converting enzyme (ACE) activity, and have potential for preventing or treating hypertension.

The invention discloses an algae-derived functional active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequence of the algae-derived functional active peptide is at least one of FPRFR and FLF. According to the preparation method, two bioactive peptides with the effects of resisting oxidation and reducing uric acid are obtained through separation and purification by taking micrococcus as a raw material, chemical synthesis is carried out according to an identified peptide fragment sequence, the effects of reducing uric acid and resisting oxidation of a synthesized single peptide fragment and a compounded peptide fragment are determined, and the bioactive peptides have relatively good XOD inhibition rate and DPPH clearance rate. Therefore, the algae-derived functional active peptides not only can be used as natural antioxidants in food, medicines and / or cosmetics, but also can be used as natural uric acid-reducing supplements in medicines; the method has an important application prospect in the development of products with the effects of reducing uric acid and resisting oxidation.

The invention belongs to the technical field of biological medicines and bioactive peptides, and relates to a xanthine oxidase inhibitory peptide derived from goat milk protein and application thereof. The amino acid sequence of the xanthine oxidase inhibitory peptide is as shown in SEQ ID NO. 1. The xanthine oxidase inhibitory peptide can be used for preparing a product for inhibiting xanthine oxidase or a product for regulating and controlling the uric acid level in a human body. The XOD regulation active peptide provided by the invention is safe in source and clear in structure, can be applied to the field of functional food or nutrition intervention, and provides a safe and natural active substance basis for diet regulation of hyperuricemia and related metabolic diseases.