455 results about "Bioactive peptide" patented technology

The invention relates to the technical field of bioactive peptides, in particular to a novel multifunctional collagen-like tripeptide and application thereof, an amino acid sequence contains multiple combinations, X can be selected from multiple components, 3-methoxy-4-hydroxyphenylalanine and the like are added, and the novel multifunctional collagen-like tripeptide can interact with specific proteins, activate related pathways and play multiple physiological functions and is applied to multiple fields. The collagen-like tripeptide has obvious advantages, innovates component expansion functions, improves the preparation method, improves the efficiency, reduces the cost, is matched with other components in the fields of beauty, health care and medicine, enhances the efficacy, realizes targeted delivery, and brings a new opportunity to related industries.

The invention discloses a fish by-product active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequences of the fish by-product active peptide are shown as SEQ ID NO: 1 to SEQ ID NO: 3. Both the single peptide fragment and the compound peptide fragment of the fish by-product active peptide can obviously inhibit NO secretion of a lipopolysaccharide (LPS)-induced macrophage (RAW264.7) inflammation model, so that the fish by-product active peptide has a relatively good application prospect in preparation of a product with an anti-inflammatory effect; and a foundation is laid for high-added-value utilization of salmon byproducts and promotion of research, development and application of functional active peptides.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

The invention discloses a blood protein precise active peptide capable of tonifying qi and blood, improving microcirculation and promoting vasodilation and a preparation method and application thereof, and belongs to the technical field of blood protein peptide. The blood protein precision active peptide comprises at least one of LGFP, IGFP, FPHFN, LFL, LFI, FESF, ILF, LIF, FLL, FIL, FII or FPHFD. The prepared blood protein precision active peptide has multiple biological activities: an antihypertensive effect is achieved by inhibiting ACE and vasoconstriction substances; vasodilation is promoted by activating a PI3K / AKT / eNOS signal channel; a zebra fish model proves that the traditional Chinese medicine composition has the effects of remarkably improving microcirculation and tonifying qi and blood. The preparation method is low in equipment requirement, simple in process, easy to operate and convenient for industrial large-scale production. The method enriches a bioactive peptide library, and is of great significance in promoting technical progress and industrial upgrading in the field of hemoglobulin peptides.

The invention relates to a bioactive polypeptide GIVVVPGSGF as well as a preparation method and application thereof. The bioactive polypeptide GIVVVPGSGF provided by the invention has bitter taste, the crystal of the bioactive polypeptide GIVVVPGSGF is powdery, and the bioactive polypeptide GIVVVPGSGF can be combined with a bitter taste receptor TAS2R14. The bioactive polypeptide can be used as a bitter additive and an additive for foods and beverages, and the beverages comprise alcoholic beverages and non-alcoholic beverages. The bioactive peptide GIVVVPGSGF provided by the invention is a small-molecular-weight peptide screened from yellow wine, sensory evaluation and electronic tongue results show that the extracted bitter peptide has good bitter taste and richness, on one hand, the bioactive peptide GIVVVPGSGF has high bitter taste strength, can properly improve the bitter taste of wine, and may increase the flavor quality under certain conditions; on the other hand, the bitter peptide GIVVVPGSGF in the yellow rice wine is properly removed, so that the evaluation experience of the yellow rice wine can be improved.

The invention discloses a corn active peptide with effects of dispelling effects of alcohol, reducing uric acid and resisting oxidation, a preparation and application of the corn active peptide, and belongs to the technical field of biological active peptides. The amino acid sequence of the corn active peptide is at least one of LMFP, FEGLFR, FLR and QLPSYR. The corn active peptide with the effects of dispelling the effects of alcohol, reducing uric acid and resisting oxidation is separated from corn germ meal through enzymolysis, ultrafiltration and other technologies, amino acid sequence determination is conducted on the separated peptide fragments, screening is conducted in combination with the bioinformatics technology, and finally four peptide fragments are obtained. According to the present invention, the corn active peptides are identified, chemical synthesis is performed according to the identified peptide fragment sequence, the effects of alcohol effect dispelling, uric acid reducing and oxidation resistance of the synthesized single peptide fragment and the compounded peptide fragment are determined, and the corn active peptides have good XOD inhibition rate, good DPPH clearance rate and good ADH activation rate, and have important application prospects in the development of the products with effects of alcohol effect dispelling, uric acid reducing and oxidation resistance.

The invention discloses a marine-derived bioactive peptide with a blood sugar reducing effect, a preparation and application, and belongs to the technical field of bioactive peptides. The amino acid sequence of the bioactive peptide is at least one of FYP, FPF and YAPFPK. Three bioactive peptides with the blood sugar reducing effect are separated and purified from oysters through enzymolysis, ultrafiltration, gel chromatography, liquid chromatography-mass spectrometry and bioinformatics technologies. According to the present invention, chemical synthesis is performed according to the identified peptide fragment sequence, the hypoglycemic activity of the synthesized single peptide fragment and the compounded peptide fragment is detected, and the bioactive peptides have good alpha-glucosidase inhibition activity, such that the bioactive peptides have good application prospects in the preparation of the products with the hypoglycemic effect, and can be widely used in the preparation of the products with the hypoglycemic effect. And a foundation is laid for high value-added utilization of oysters and promotion of research, development and application of functional active peptides.

The invention particularly relates to a ginseng peptide with anti-aging activity and a preparation method and application thereof, and belongs to the technical field of bioactive peptides. The method comprises the following steps: by taking ginseng fibrous roots, ginseng leaves and other by-products as raw materials, carrying out weak acid ultrasonic pretreatment, loading natural anti-aging active components, carrying out low-temperature high-pressure homogenization and airflow crushing synergistic treatment, carrying out double-stage composite enzymolysis, clarifying with an alternating electric field ceramic membrane, carrying out five-stage series ultrafiltration classification, carrying out four-stage filter element sterilization, freeze-drying and the like in sequence. The high-purity ginseng peptide is prepared. The total nitrogen content of the prepared ginseng peptide is larger than or equal to 14.8%, the proportion of a target peptide fragment is larger than or equal to 97.2%, the yield is larger than or equal to 18.5%, and the ginseng peptide has good antioxidant and anti-aging activity and can be used for development of functional food and skin care products. The method is low in cost, high in resource utilization rate and green and efficient in process, and the product purity and activity are remarkably superior to those of a traditional method.

The invention relates to the technical field of deep learning, and discloses a multifunctional bioactive peptide function classification method based on evolutionary deep learning. Comprising the following steps: acquiring a data sample set, and dividing the data sample set into a training set and a test set according to a preset proportion; preprocessing the training set and the test set to obtain a preprocessed training set and a preprocessed test set; constructing an initial deep learning model for active peptide function classification, searching parameters of the model by using an evolutionary algorithm, and constructing an optimized deep learning model based on the parameters; training an optimized deep learning model by using the preprocessed training set, and testing the trained optimized deep learning model by using the preprocessed test set to obtain a trained deep learning model; and preprocessing a to-be-classified peptide sequence, and inputting the preprocessed peptide sequence into the trained deep learning model to obtain a classification result. By adopting the method, the classification precision of the active peptide and the migration ability of the model to adapt to different data sets can be improved.

The invention discloses anti-inflammatory polypeptide nanoparticles and a preparation method thereof, and belongs to the technical field of bioactive peptides. The anti-inflammatory polypeptide nano-particles are nano-particles obtained by coating polypeptide with an anti-inflammatory effect with chitosan and sodium tripolyphosphate, the polypeptide with the anti-inflammatory effect contains bioactive peptides of which the amino acid sequences are YAADQ, FPYTT and YVTYA. The phycocyanin anti-inflammatory peptide is natural in source, the performance of the phycocyanin anti-inflammatory peptide can be remarkably improved through the nanometer embedding technology, and compared with traditional drugs, the phycocyanin anti-inflammatory peptide has lower toxic and side effects and potential cost advantages. The polypeptide nanoparticles prepared by the invention can effectively prevent the phycocyanin anti-inflammatory peptide from being degraded in the digestive tract, have a remarkable treatment effect on mice with ulcerative colitis, and have an important application prospect in preparation of ulcerative colitis prevention and treatment products.

Disclosed herein is an adenoviral vector system utilizing DARPin adapters. The system is highly effective, safe and able to deliver DNA in a cell-specific manner. It is demonstrated that the system is unexpectedly versatile, and can be used in conjunction with protein scaffolds, bioactive peptides and small molecules. This makes the system useful for numerous purposes, including the use of the system for therapeutic and diagnostic purposes.

Provided herein are peptide amphiphiles (PAs) comprising a bioactive peptide, nanofibers displaying the bioactive PAs, and methods of use thereof. The disclosed peptide amphiphiles comprise a hydrophobic tail, a structural peptide segment, a charged peptide segment, and a bioactive IKVAV peptide. The disclosed PAs may be used in cell culture methods and in methods of treating central nervous system injury.

The invention discloses a scallop skirt ACE inhibitory peptide activity method and system based on deep learning, and relates to the technical field of bioengineering.The scallop skirt ACE inhibitory peptide activity method comprises the steps that a comprehensive data set is obtained based on simulated in-vivo test data and peptide level feature data of different peptides, key features are screened, and a training set and a test set are divided; initializing a random forest model, an SVM model and a logistic regression model, performing independent training by using the training set, and determining an optimal prediction model of the peptide antihypertensive activity based on a prediction result of the test set; extracting importance data of key features based on the optimal prediction model for the antihypertensive activity of the peptide, sorting the importance data, and visually analyzing the antihypertensive activity features, feature importance sorting and model performance of different peptides; and all key results in the analysis process are integrated and systematically stored. According to the method, normal form reference with prediction accuracy and mechanism depth can be provided for clinical transformation of marine bioactive peptides, and AI-driven novel peptide drug research and development are promoted to enter a new stage.

The invention discloses a preparation method of crocodile liver peptide for improving liver injury, and relates to the technical field of bioactive peptide extraction, and the preparation method comprises the following steps: crocodile liver pretreatment: cutting fresh crocodile liver into blocks, mixing with deionized water, homogenizing, and adding a zirconium phosphate molecular sieve to obtain crocodile liver homogenate; enzymatic hydrolysis reaction: adjusting the pH value of the homogenate, and adding a compound enzyme preparation for enzymatic hydrolysis; carrying out enzyme deactivation treatment, namely heating, cooling, centrifuging and taking supernate; performing ultrafiltration separation, namely filtering the supernate through an ultrafiltration membrane, and collecting a small molecule peptide solution; purifying and refining: purifying through sephadex column chromatography, and freeze-drying to obtain a crocodile liver peptide product; a double-particle-size zirconium phosphate molecular sieve is constructed to cooperate with a compound enzyme preparation for enzymolysis, electron transfer is enhanced by combining phosphorylation modification, the defects of metal inhibition enzyme activity, insufficient concealed peptide fragment cutting and oxidative damage are overcome, meanwhile, the application of the crocodile liver peptide in the aspect of improving the liver function is researched, and the crocodile liver peptide has a good application prospect. And a new choice is provided for prevention and treatment of liver diseases and liver health care.

The invention relates to an application of a bioactive peptide YPFPGPIH derived from bovine casein in the aspect of anti-inflammation or immunoregulation. The peptide can be combined with TLR2 and TLR4 to inhibit the activation of NF-kappa B and MAPK signal channels, so that the levels of inflammatory factors such as IL-1beta, TNF-alpha and NO are remarkably reduced, the expression of IL-10 is up-regulated, and inflammation balance regulation is realized; the body inflammatory response is improved, and the immune organ injury is recovered. The peptide can be used for preparing anti-inflammatory drugs, immunomodulatory functional foods and nutritional supplements, and has good safety and application prospects.

The present application relates to the technical field of polypeptide, and discloses a pearl producing Pinctada martensii with whitening and antioxidant effects, a preparation method and application thereof, wherein the pearl producing Pinctada martensii is a mixture of short peptides, the pearl producing Pinctada martensii takes pearl layer powder of Pinctada martensii as raw material, water and composite ionic liquid are added in proportion, and after physical modification under high temperature and high pressure, the pearl producing Pinctada martensii is sequentially subjected to complex degradation by bromelain and central protease, enzyme inactivation, centrifugation, separation and purification, and identification. The pearl producing Pinctada martensii prepared by the present application has the effects of whitening and free radical scavenging, and the present application provides a new type of bioactive peptide for the field of cosmetics, and simultaneously improves the application value of pearl layer powder in the fields of biological medicine and food.

The invention belongs to the field of functional polypeptide preparation, and particularly relates to euphausia superba peptide and application thereof in preparation of immunomodulatory drugs. The euphausia superba peptide is prepared from the following peptide fragments: DIFDPL (Diaphragmitic Induced Factor Dinucleotide Lipoprotein Lipoprotein), KPWALT (Keylanine White Amplified Transcription), LPFQRL (Lipoprotein Lipoprotein Quantitative Reducing Lipoprotein), FYFF (Factor Yeast Factor The Antarctic krill mixed peptide sample prepared by the invention has no cytotoxicity, and can promote cell proliferation, enhance cell NO secretion, improve cell phagocytic ability and effectively enhance immunity. The euphausia superba peptide prepared in the invention is easier to absorb, and has significant reduction and oxidation resistance. The polypeptide provided by the invention is a functional influence factor which has huge potential in the aspect of enhancing immunity, different bioactive peptides have different action mechanisms due to amino acid composition and structural characteristics, and theoretical support is provided for further developing food for enhancing the immunity function by utilizing the euphausia superba peptide. The development value of the euphausia superba is improved.

The invention belongs to the technical field of bioactive peptides, and discloses a white snakehead oligopeptide as well as a preparation method and application thereof. The white snakehead oligopeptide is selected from one of oligopeptides with amino acid sequences as shown in SEQ ID NO.1-8. The invention further discloses a preparation method of the white snakehead oligopeptide. The polypeptide provided by the invention has a relatively high ferrous / zinc absorption rate to a human body, has a certain function of treating iron-deficiency anemia and zinc deficiency, and has a good application prospect in mineral (iron / zinc) supplements, functional foods and health-care products.

Use of an effective amount of the bioactive peptide in the preparation of a medicament for the prevention and / or treatment of hypoxic-ischemic encephalopathy in newborns, wherein the amino acid sequence of the bioactive peptide contains KKNRNKLRRQHSY or a functionally equivalent variant thereof. Optionally, the bioactive peptide further comprises a signal peptide at its N-terminus or C-terminus.

The invention discloses a preparation process of ready to dye ready to dye and easy to dye easy to dye hair, and particularly relates to the technical field of hair treatment chemistry, the process is realized through three stages of pretreatment, modification treatment and neutralizing and shaping which are sequentially executed, the hair is immersed in a solution containing an alkaline reagent system and a bioactive peptide structure enhancer, and then the hair is soaked in a solution containing an alkaline reagent system and a bioactive peptide structure enhancer; at a specific temperature, synchronously opening hair cuticles and repairing keratin disulfide bond damage; under the catalysis of a rare earth metal complex catalyst, a permanent cationization three-dimensional network is formed through covalent grafting of an amphiphilic quaternary ammonium salt compound and a keratin nucleophilic site; a neutralizing solution containing an organic acid system and reactive siloxane is adopted to close hair cuticles, an ionic bond anchored hydrophobic protective film is formed, a biological peptide synergistic repair mechanism effectively overcomes keratin damage caused by traditional alkali treatment, and the completeness of the hair cuticles is remarkably improved; a high-density permanent cation site is constructed through rare earth catalytic covalent grafting, so that the dye adsorption strength is improved, and the problem that hair dyeing process damage and durability are difficult to consider at the same time is solved.

This invention discloses an anti-inflammatory peptide derived from pearl oysters and its applications, relating to the field of bioactive peptide technology. The amino acid sequence of this anti-inflammatory peptide is TAMY. The anti-inflammatory peptide derived from pearl oysters provided by this invention can regulate the release of cellular inflammatory mediators, reduce the levels of NO and pro-inflammatory cytokines (such as TNF-α, IL-6, and IL-1β), and simultaneously promote the secretion of anti-inflammatory cytokines (such as IL-10), exhibiting excellent anti-inflammatory activity and suitable for use in functional foods, cosmetics, or pharmaceutical ingredients.

The invention discloses an eggshell membrane source antioxidant umami peptide and application thereof, the amino acid sequence of the eggshell membrane source umami peptide is at least one of CDDDF, CWDVYR and WHDCHR, and relates to the technical field of bioactive peptides. The eggshell membrane umami peptides with antioxidant activity are screened by using a method of combining virtual enzymolysis, machine learning, molecular docking and molecular dynamics simulation, and three eggshell membrane umami peptides are screened and can be combined with umami receptor T1R1 / T1R3 subunits, and the composite conformation is stable. Sensory evaluation and electronic tongue analysis prove that the eggshell membrane source antioxidant umami peptide has good umami characteristics, the umami threshold value is 0.04-0.10 mmol / L, the eggshell membrane source antioxidant umami peptide has DPPH free radical scavenging capacity, ABTS + free radical scavenging capacity and Fe < 3 + > reduction activity, a new raw material is provided for development of functional seasonings, and the eggshell membrane source antioxidant umami peptide has important application value.

The invention relates to the technical field of functional beverages, in particular to a composite polypeptide solid beverage with a uric acid reducing function and a preparation method of the composite polypeptide solid beverage. The composite polypeptide solid beverage with the uric acid reducing function is prepared from the following raw materials in percentage by weight: 15-25% of plukenetia volubilis extract, 30-40% of lysimachia christinae hance-plantain herb composite extract, 8-12% of oyster peptide powder, 5-8% of earthworm protein peptide freeze-dried powder and 3-5% of a flavoring agent. Wherein the ratio of a lysimachia christinae hance extract to a plantain herb extract in the lysimachia christinae hance-plantain herb composite extract is 3: The flavoring agent is erythritol and / or stevioside, xanthine oxidase is inhibited by using flavonoid substances, GLUT9 expression is synergistically down-regulated by using bispyribaloside, PRPP synthetase activity is regulated by using zinc-rich peptide, uric acid micro-crystals are dissolved by using earthworm protein peptide, a multi-target action network of flavonoid-glycoside-bioactive peptide is achieved, and the effect of improving the bioavailability of the flavonoid-glycoside-bioactive peptide is achieved. And triple mechanisms of excretion promotion, generation inhibition and kidney protection are realized.

The application discloses a milk-derived polypeptide VSL for promoting proliferation of skeletal muscle cells and application thereof, and belongs to the field of bioactive peptides. The milk-derived bioactive polypeptide VSL has the effect of promoting proliferation of muscle cells, and is expected to be used for preparing drugs for promoting proliferation of muscle cells and preventing sarcopenia. The milk-derived bioactive polypeptide VSL is convenient to synthesize, can be produced industrially, and has a good application prospect in the fields of food, medicine and cosmetics.

The invention belongs to the technical field of bioactive peptides, and particularly relates to a phycocyanin peptide and application thereof in preparation of a product with functions of inhibiting PTGS2 expression and regulating an Akt / GSK3beta signal channel. According to the invention, phycocyanin is taken as a raw material, active polypeptides with specific sequences are obtained through an enzymolysis separation technology, and the amino acid sequences of the active polypeptides are respectively Leuu-Ala-Ser and Leuu-Gly-Leu. Experiments prove that the phycocyanin peptide prepared by the invention can significantly inhibit the expression of PTGS2, and can play a related regulation role by activating an Akt / GSK3beta signal channel, so that the technical blank of plant active peptide targeting PTGS2 and Akt / GSK3beta signal channels is filled, and the phycocyanin peptide can be widely applied to the development of related products for regulating the abnormal expression of PTGS2 and Akt / GSK3beta signal channels.

The present disclosure provides a nanobody platform having a high affinity purification on protein A, wherein the nanobodies may incorporate diverse bioactive peptides, lipid or glycoside in its CDR1 and / or CDR3 regions. The present disclosure also provides a peptide-modified nanobody comprising non-toxic bioactive peptides and that exhibit potent anti-tumor activity. Moreover, the peptide-modified nanobody is able to be combined with other technologies, such as but not limited to bispecific antibodies and antibody-drug conjugates. Further, the present disclosure also provides production processes of the nanobodies and their use as a treatment and diagnostic agents.

This invention discloses the XO-inhibiting peptide GDEY and its applications, belonging to the field of bioactive peptide technology. The amino acid sequence of the XO-inhibiting peptide GDEY is shown in SEQ ID NO.2. The invention also discloses the application of the XO-inhibiting peptide GDEY in the preparation of xanthine oxidase inhibitors and in the preparation of drugs with the effect of inhibiting uric acid levels. This invention used molecular docking and BLAST+ software to virtually screen 15 potential XO-inhibiting peptides from the complete protein sequence of Litopenaeus vannamei. High-performance liquid chromatography (HPLC) was used to determine the XO-inhibiting activity, and the results showed that 7 small molecule peptides exhibited significant XO-inhibiting activity, possessing potential value in preventing hyperuricemia.

The invention relates to a bioactive polypeptide IVVLPTGVAH as well as a preparation method and application of the bioactive polypeptide IVVLPTGVAH. The bioactive polypeptide IVVLPTGVAH provided by the invention has bitter taste, the crystal of the bioactive polypeptide IVVLPTGVAH is powdery, and the bioactive polypeptide IVVLPTGVAH can be combined with a bitter taste receptor TAS2R14. The bioactive polypeptide can be used as a bitter additive and an additive for foods and beverages, and the beverages comprise alcoholic beverages and non-alcoholic beverages. The bioactive peptide IVVLPTGVAH provided by the invention is a small-molecular-weight peptide screened from yellow wine, sensory evaluation and electronic tongue results show that the extracted bitter peptide has good bitter taste and richness, on one hand, the bioactive peptide IVVLPTGVAH has high bitter taste strength, the bitter taste of wine can be properly improved, and the flavor quality can be possibly improved under certain conditions; on the other hand, the bitter peptide IVVLPTGVAH in the yellow rice wine is properly removed, so that the evaluation experience of the yellow rice wine can be improved.

The invention provides a method for efficiently preparing active polypeptide by fermenting rapeseed dregs, which comprises the following steps: pretreatment: crushing rapeseed dregs, adding 3-5% of reducing sugar, humidifying and sterilizing; performing mixed bacteria segmented fermentation: inoculating a composite strain consisting of bacillus licheniformis SWJS33 with high yield of aminopeptidase, aspergillus oryzae and lactobacillus plantarum, and fermenting at 30 DEG C or 37 DEG C for 72-84 hours; ultrasonic wall breaking: adding water after fermentation, uniformly mixing, carrying out ultrasonic treatment for 20-30 minutes under the power of 120-200W, and breaking thalli to release intracellular enzyme; enzymolysis: carrying out enzymolysis for 4-6 hours under the condition of 50-60 DEG C; post-treatment: enzyme deactivation, centrifugation and drying are carried out after enzymolysis, and a polypeptide product is obtained. By adding the exogenous reducing sugar, nutrition is enhanced, and thallus proliferation is accelerated; bacillus licheniformis SWJS33 with high yield of aminopeptidase is inoculated, so that the generation of small-molecule active peptide is further promoted; a mixed bacteria composite system and a segmented fermentation technology are adopted, and bacteria cells are crushed in an ultrasonic-assisted manner, so that the enzymolysis efficiency is enhanced, and the yield and activity of the polypeptide are remarkably improved.

Described herein are isolated peptides, compositions comprising the same, and methods of using such peptides or compositions in the treatment of depression, central nervous system disorders, and neurodevelopmental disorders.