296 results about "Ace inhibition" patented technology

Disclosed are compositions containing a VDRA/Vitamin D analog to treat or prevent kidney disease, including chronic kidney disease. The present invention also relates to methods of treating kidney disease by administering to a patient a pharmaceutical composition containing a therapeutically effective amount of a VDRA/Vitamin D analog. Compositions according to the invention include a VDRA/Vitamin D analog and at least one of the following agents: an ACE inhibitor, an angiotensin (II) receptor blocker (ARB) and aldosterone blocker in therapeutically effective amounts to inhibit renin production or inhibit activation of the renin-angiotensin-aldosterone system. Preferred compositions contain paricalcitol with at least one of these other agents. Such combinations can avoid ACE inhibition escape and aldosterone escape with subsequent increase in angiotensin (II) and aldosterone generation.

Disclosed are compositions containing a VDRA/Vitamin D analog to treat or prevent kidney disease, including chronic kidney disease. The present invention also relates to methods of treating kidney disease by administering to a patient a pharmaceutical composition containing a therapeutically effective amount of a VDRA/Vitamin D analog. Compositions according to the invention include a VDRA/Vitamin D analog and at least one of the following agents: an ACE inhibitor, an angiotensin (II) receptor blocker (ARB) and aldosterone blocker in therapeutically effective amounts to inhibit renin production or inhibit activation of the renin-angiotensin-aldosterone system. Preferred compositions contain paricalcitol with at least one of these other agents. Such combinations can avoid ACE inhibition escape and aldosterone escape with subsequent increase in angiotensin (II) and aldosterone generation.

The invention discloses a novel orally-effective spirulina source antihypertensive peptide and a preparation method thereof. The antihypertensive peptide is a tripeptide with the sequence of isoleucine-glutamine-proline (Ile-Gln-Pro, IQP). An angiotensin converting enzyme (ACE) inhibitory peptide is prepared by the following steps of: performing enzymolysis on spirulina platensis by adopting alkali protease, and performing ultrafiltration, gel filtration chromatography and secondary reversed phase high-performance liquid chromatography purification. Tests prove that the IQP is a noncompetitive inhibitor of ACE, and can keep good ACE inhibitory activity under digestion of main gastrointestinal protease. The IQP has good antihypertensive effect on essential hypertension rat (SHR) and has great application prospects in medicaments and functional foods for preventing and treating hypertension.

The invention discloses a yak skin blood pressure-decreasing collagen polypeptide and a preparation method thereof. The preparation comprises the following steps: pre-treating raw materials by virtue of physical and chemical methods, preparing gelatin, preparing ACE-inhibiting collagen polypeptide by virtue of composite enzymatic hydrolysis, and separating and purifying polypeptide by utilizing methods such as ultra-filtration, ion-exchange column chromatography, gel filtration chromatography and high performance liquid chromatography; concentrating and drying. According to the technical scheme in the invention, collagen polypeptide is successfully extracted from the yak skin, and the collagen polypeptide has good solubility, emulsifying capacity and foaming characteristic, and has higher ACE-inhibiting activity (blood pressure decreasing effect); the preparation method is simple and easy to operate, and low in cost, so that the application prospect of the yak skin is expanded.

The invention provides a preparation method of a cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor. The preparation method comprises the steps of A, weighing kernel meal of degreased cannabis sativa; drying; crushing; sieving to remove impurities; feeding water until reaching fixed material-liquid ratio; homogenizing through a colloid mill; sieving; regulating the pH value; ultrasonically assisting to extract; extracting protein with alkali under a constant temperature; performing isoelectric precipitation; centrifuging to remove supernate; extracting protein from precipitate with alkali for the second time; feeding water to reach the fixed material-liquid ratio; homogenizing through a homogenizing machine; sieving; regulating the pH value; performing isoelectric precipitation again; centrifuging to remove supernate; neutralizing; drying to obtain separated-out protein of kernel meal of cannabis sativa; B, feeding deionized water to separated-out protein of kernel meal of cannabis satival; regulating pH; feeding mixed protease for enzymolysis under the constant temperature; deactivating enzyme by boiling water bath; centrifuging to obtain supernate; C, separating and purifying protease enzymolysis solution of kernel meal of cannabis satival; collecting to obtain the ACE peptide inhibitor of protein of kernel meal of cannabis satival; freezing and drying to obtain the cannabis sativa protein ACE peptide inhibitor. The method has the advantages that the operation is simple, the product yield is increased, and the ACE inhibition effect is excellent.

The invention relates to development and utilization of maize polypeptide products and discloses a method for preparing maize polypeptide through synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis by immobilized enzyme, belonging to the technical field of deep processing of maize. The method comprises (1) a preparation process for sodium alginate-immobilized alkali protease; and (2) a process for synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis of zein by immobilized enzyme. According to the invention, synchronous multi-frequency ultrasonic wave and immobilized enzyme combined technology is employed for enzymatic hydrolysis and preparation of the polypeptide product, so the method provided by the invention has the following advantages compared with conventional methods utilizing enzymatic hydrolysis, single-frequency ultrasonic-aided enzymatic hydrolysis and double-frequency ultrasonic-aided enzymatic hydrolysis: the prepared maize polypeptide has higher ACE inhibitory activity, and the yield and activity of the polypeptide are better improved. The immobilized enzyme is recoverable; the repeated utilization rate of the enzyme is higher; production cost is better saved; and a feasible approach is provided for industrial production of a high-added-value maize polypeptide product.

The invention discloses a lactoalbumin antihypertensive peptide and a preparation method and an application thereof. The lactoalbumin antihypertensive peptide is prepared by the following steps: after hydrating lactoalbumin solution, adjusting the pH value to 7.0-10.0, adding protease I for enzymolysis reaction; after reacting for 120-240 minutes, keeping reaction temperature constant; adjusting the pH value to 6.0-8.0; adding protease II into a reaction system to continue reacting; after reacting for 120-240 minutes, stopping reacting to obtain rough enzyme solution; inactivating enzyme in the rough enzyme solution, and adjusting the pH value to the isoelectric point of lactoalbumin; centrifuging to obtain supernate; adjusting the pH value of the supernate to 6.0-8.0; and collecting ultrafiltrate by a 10 kDa ultrafiltration membrane; and obtaining lactoalbumin antihypertensive peptide. Product analysis indicates that the prepared lactoalbumin antihypertensive peptide contains less than 2% of ash content, protein recovery rate approaches to 90%, TCA soluble nitrogen content is more than 85%, free amino acid content is 70.23 mu mol/mL, and ACE suppression ratio is more than 85%.

The invention discloses a method for preparing antihypertensive peptides through enzymolysis of ground meat proteins of a tuna. The method comprises the steps of: (1) regarding the ground meat of the tuna as the raw material, mixing and homogenizing the raw material and a buffer solution according to a weight/volume ratio of 1 g: 3-5 ml, and then adjusting the pH value to 9.5-10 to obtain a mixed solution; (2) heating the mixed solution to 55-60 DEG C for pre-heating, and adding alcalase according to 1.0%-1.5% of the raw material for enzymolysis at an enzymolysis temperature of 55-60 DEG C for 4-6 h; and (3) performing enzyme deactivation on the obtained enzymolysis product to obtain an enzymolysis solution and then enabling the enzymolysis solution to sequentially suffer from ultrafiltration, desalination, freeze drying and chromatography to obtain the antihypertensive peptides. The method for preparing the antihypertensive peptides through the enzymolysis of the ground meat proteins of the tuna, disclosed by the invention, has the advantages of abundant source of the raw material, low price, simple preparation process, relatively strong activity of the prepared antihypertensive peptides; compared with a chemosynthetic ACE (Angiotensin Converting Enzyme) inhibitor, the ACEP (ACE Inhibitive Bioactive Peptide) prepared through enzymolysis of the ground meat of the tuna has the advantages of being safe and nontoxic, specific in antihypertensive efficacy, easy to digest and absorb and the like.

A depressor peptide with higher suppression activity to angiotensin-converting enzyme (ACE) and having 6 amino acids is prepared from the protein of Chinese shrimp through enzymolyzing, separation and purifying. Its short peptide with 12 sequences is chemically synthesized. It can be used to prepare antihypertensive medicine.

The invention discloses a method for extracting silk peptide from cocoon fiber, which belongs to the technical field of preparation of the silk peptide. The method comprises the following steps: the cocoon fiber is added into a Na2CO3 solution for refining, filtrated and added into a CaCl2 solution for dissolution; protease for silk dissolution solution obtained after dissolution undergoes enzymatic hydrolysis reaction; after ultrafiltraiotn and nano-filtration separation of digest, a feed liquid is obtained; and the silk peptide powder is obtained after spray drying of the feed liquid. The method has the advantages that ACE inhibitory polypeptide is enriched through ultrafiltraiotn and nano-filtration separation of the digest; the efficiency of removing CaCl2 salt is high; the method has the advantages of large processing capacity, mild separation condition, simple flow, low energy consumption and so on; and the industrial production is easy to realize.

The invention provides a loach protein antihypertensive peptide and a preparation method thereof, belonging to the technical field of biology. The antihypertensive short peptide separated and purified from an enzymolysis product of loach protein has high activity in inhibiting angiotensin converting enzyme (ACE). The preparation method provided by the invention comprises the following steps of: carrying out enzymolysis on the loach protein to prepare an enzymolysis liquid; separating and purifying the antihypertensive peptide from the enzymolysis liquid; and sequencing the antihypertensive peptide. The invention combines a modern enzyme engineering technique and a peptide purifying and identifying technique, deep development and utilization on the loach protein are utilized, and a novel-sequence short peptide Leu-Glue-Tyr with high ACE inhibiting activity is found; and the novel loach protein antihypertensive peptide provided by the invention has high ACE inhibiting activity and has an IC50 value of 0.103 mg/mL.

The invention discloses a preparation method of rice bran protein peptide with ACE inhibitory activity. The method is characterized in that rice bran is adopted as a raw material, and comprises the following steps: firstly extracting rice bran protein, then preparing rice bran protein hydrolysate by adopting an enzyme hydrolysis method, and separating and purifying the rice bran ACE inhibitory peptide by adopting a hyperfiltration method, sephadex G-15 and a RP-HPLC method. According to the preparation method, the rich and cheap rice bran resource is effectively utilized to prepare the rice bran protein peptide with the high ACE inhibitory activity. The decompression function of the rice bran ACE inhibitory peptide can be maximally utilized, so that on one hand, the additional value of the rice bran is increased, and the good economic benefit and market value can be created; on the other hand, the morbidity of the hypertension can be effectively reduced, the high morbidity can be controlled, the human beings can be protected from the harm of the hypertension, the application value and social benefit are good, and the theoretical foundation and the experiment evidence can be provided for researching the structure and decompression mechanism of the rice bran protein ACE inhibitory peptide.

The invention relates to Lactobacillus helveticus H9 and application of the Lactobacillus helveticus H9 to the resistance of hypertension and the prevention of cardiovascular disease. A preparation method comprises the following steps of: isolating strains, identifying the strains, preserving the strains, culturing the strains, preparing a bacterial liquid, preparing fermented milk, and screening a strain with angiotensin-converting enzyme (ACE) inhibitory activity; and further evaluating the influence of Lactobacillus helveticus H9 fermented milk on hypertension (SHR, spontaneously hypertensive rats) and normal blood pressure (WHY rats, Wistar-Kyoto rats). The research shows that strain fermented milk has a better in-vivo antihypertensive effect, has the advantages of long onset time and the like, does not affect blood circulation, and has no adverse effects on the normal blood pressure.

The invention discloses a method for separating and purifying peanut bioactive peptide. The method comprises the steps that: peanut protein power protease enzymatic hydrolysate is prepared; the enzymatic hydrolysate is nanofiltrated; the filtrate is pre-condensed, such that a pre-condensed peanut protein power protease enzymatic hydrolysate is obtained; the pre-condensed enzymatic hydrolysate is desalted by a resin column; condensed solution is collected, such that desalted peanut protein power protease enzymatic hydrolysate is obtained; the desalted enzymatic hydrolysate is ultrafiltrated, separated, and condensed; the condensed solution is collected and lyophilized, such that peanut bioactive peptide is obtained. Peanut bioactive peptide provided by the invention has high purity, and has good functional activities of oxidative stability, bacteriostasis and ACE inhibitive activity. The peanut bioactive peptide can be applied in foodstuff industries as a natural foodstuff additive, and can be applied in auxiliary treatment of hypertension as a natural functional healthcare food material. The method has advantages of simple technology and easy operation, and is suitable for industrialized productions.

The invention aims at providing a novel angiotensin-converting enzyme inhibition peptide, namely an ACE inhibition peptide prepared form isochrysis galbana, and with the amino acid sequence of SEQ ID NO:1. The invention provides a preparation method of a high-activity angiotensin-converting enzyme inhibition peptide sourcing from marine microalgae. The current angiotensin-converting enzyme inhibiter mainly comes from food and large macroalgae of laver and the like, and coming from bait microalgae is not reported. The structure of the obtained active peptide with a unique structure is Tyr-Met-Gly-Leu-Asp-Leu-Lys. The structure is novel and has the characteristics of being small in molecular weight and easily absorbed by a human body.

Milk ACE inhibiting peptide is prepared by: hydrolyzing milk casein as raw material with protease, heating the hydrolytic liquid to inactivate protease, centrifugally separating to obtain supernatant, desalting with ion exchange resin, freezing to dry and obtain high active edible ACE inhibiting peptide. The product can be used as food additive as functional factor or as food supplementary to add into various foods.

Shark protein antigypertensive peptide, preparation and application thereof, which pertains to marine biology technical field. The novel antigypertensive peptide with two amino acid separated and purified from proteolysis product of shark, has a high inhibiting activity for angiotensin converting enzyme (ACE). Preparation includes steps of carrying out enzymolysis for shark protein to prepare enzymatic hydrolysate, seperating and purifying peptide from the enzymatic hydrolysate, and sequencing of peptide. The invention is applicable for preparing antihypertensive medicament. The invention apply microbial enzyme technology to deep development of shark, obtain peptide having novel sequence, with high inhibiting activity for ACE.

The invention discloses walnut peptide having the ACE inhibitory activity and a preparation method thereof. The amino acid sequence of the walnut peptide is EPNGLLLPGY, and the preparation method of the walnut peptide comprises the steps of taking walnut pomace as raw materials, selecting appropriate protease, preparing walnut antihypertensive peptide coarse products according to the optimal enzymolysis technology, adopting the coupling chromatographic technique of the membrane technology for conducting fine preparation on the walnut antihypertensive peptide, adopting the high performance liquid chromatography for conducting further purification, and adopting the vacuum freeze drying or low-temperature spray drying technology for preparing final products. According to the walnut peptide, the walnut pomace is adopted as the raw materials for developing the protein peptide, the raw materials are abundant, the price is low, and the large-scale industrialized production can be satisfied; the walnut peptide has important significance in walnut protein product diversification and development and utlization of walnut biological active peptide in functional foodstuff, health food or medicine.

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through bean pulp enzymolysis. The method comprises the following steps that (1) high-temperature bean pulp is crushed and sieved, obtained bean pulp powder and water are mixed according to a mass ratio of 1:(4-6), the pH value is regulated to 8 to 10, and a bean pulp mixture is obtained; (2) the pH value of the bean pulp mixture is regulated to 7 to 9, a complex enzyme preparation is added, the mixture is uniformly mixed, hydrolysis is carried out for 5 to 8 hours at the temperature being 35 to 50 DEG C, after the hydrolysis is completed, hydrolysis liquid is boiled in a boiling water bath for enzyme deactivation, and mixed liquid is obtained; (3) the pH value of the mixed liquid is regulated to 6.5 to 7.5, supernatant is taken, ultrafiltration treatment is performed through an ultrafiltration membrane, and filtered liquid is obtained; and (4) the filtered liquid is subjected to vacuum freezing drying, and the ACE inhibitory peptide powder is obtained. The method for preparing ACE inhibitory peptides through bean pulp enzymolysis has the advantages that the process is simple and is easy to implement; in the whole process, the reaction conditions are mild; the compounded complex enzyme preparation is used; and the industrial processing operation is convenient.

The invention provides an oligopeptide composition, and a delicious seasoning containing the same and a preparation method and application thereof. The oligopeptide composition is prepared from a shellfish or alga through enzymatic hydrolysis under the action of supersonic waves, wherein enzymatic hydrolysis conditions are that the power of the supersonic waves is 250 to 500 W, an enzyme concentration is 2 to 10 mg/g, the mass percent concentration of a substrate is 2 to 10% (in terms of the content of protein), temperature is 45 to 60 DEG C, time is 1 to 10 h and used enzymes comprises flavourzyme and one or more selected from the group consisting of compound protease, neutral protease, papain, alkali protease and pancreatin, and the molecular weight of oligopeptide in the oligopeptide composition is 200 to 4000 Da. The oligopeptide composition has high ACE inhibition activity and unique delicious taste; and the delicious seasoning with a blood pressure-reducing health function and unique flavor can be prepared from the oligopeptide composition.

The invention discloses an ACE inhibitory peptide and a preparation method thereof, and provides a casein hydrolysate which has an anti-hypertension effect and is rich in the ACE inhibitory peptide, the ACE inhibitory peptide with an anti-hypertension effect, and a preparation method of the ACE inhibitory peptide. The casein hydrolysate rich in the ACE inhibitory peptide contains two peptide fragments with ACE inhibitory activities; and the amino acid sequences of the two peptide fragments with ACE inhibitory activities are respectively YS and YQKFPQYLQY. The amino acid sequence of the ACE inhibitory peptide is YS or YQKFPQYLQY. The ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is sufficiently mixed with milk powder matrixes to prepare anti-hypertension milk powder; or the ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is mixed with fresh milk to obtain a mixture, and lactic acid bacteria is inoculated in the mixture to obtain yoghourt with an anti-hypertension effect. The dairy product has a remarkable anti-hypertension effect, has no influence on the normal blood pressure, is safe to eat, and is suitable for being ate by the hypertension groups.

The invention provides a yak bone protein peptide with an ACE inhibiting function and a preparation method and application thereof. The yak bone protein peptide is prepared from enzymatically hydrolyzed yak bone protein by multiple-step enzymatic hydrolysis by compound protease, and the compound protease consists of alkali protease, papain, trypsin and flavourzyme. The yak bone protein peptide provided by the invention has an excellent ACE inhibiting function, the ACE inhibiting activity (IC50 value) is smaller than 0.32mg/mL, and the yak bone protein peptide can be used as an ACE inhibitor to be applied to special foods and nutritional foods. The preparation method of the yak bone protein peptide provided by the invention is simple, the whole processing course is not added with acid or alkali for regulating the pH value, and the product maintains good functional characteristics and is easier to realize industrialized production.

The invention mainly relates to Larimichthys crocea connetin source ACE inhibitory peptide sequences and applications thereof. The two kinds of peptide sequences respectively are WAR and WQR, and establish a basis for prevention and treatment of hypertension. Different on-line databases are used, and after multiple times of screening, the inhibitory peptides with high efficient ACE inhibition activity are obtained. WAR and WQR are artificially synthesized, in order to verify external ACE inhibition activity of the peptides by using a high performance liquid chromatography method. The inventionrelates to two kinds of active peptides with good external ACE inhibition activity. The two kinds of peptide sequences are used for developing health food with blood pressure adjusting effects, and the purpose for prevention, control, alleviation and auxiliary treatment of hypertension is realized by long-term usage.

The invention relates to a preparation method for a deer blood active composition. The deer blood active composition is obtained by taking fresh deer blood as a raw material and performing virus inactivation, protease first enzymatic hydrolysis, saccharomyces cerevisiae secondary fermentation and drying. The deer blood active composition prepared according to the technology possesses the following characteristics: (1) large-molecular-weight protein is degraded into small molecules in the enzymatic hydrolysis process, deer blood is prevented from causing adverse reactions such as allergy, and because the peptide active site is exposed, the deer blood active composition possesses ACE inhibition activity, DPP-IV inhibition activity, antibacterial activity and other functions; and (2) after the deer blood active composition subjected to first enzymatic hydrolysis and secondary fermentation is decomposed, original fishy smell of a blood product is eliminated, and the taste is relatively easily accepted. The deer blood active composition is applicable to fields of medicines, functional food, food additives and the like, and possesses wide application prospect.

The invention provides green tea goat milk with a function of reducing blood pressure and a preparation method of the green tea goat milk. Goat milk beverage which is rich in ACE inhibitory peptides is prepared by compound enzymolysis, is low in production cost, not limited by resource and small in environmental pollution, and can be mass-produced, and products are safe. On the basis, ultra-fine green tea powder is prepared by enzymic hydrolysis of cell walls and a low-temperature ultra-fine pulverizing technology, specific surface area is increased, and a large number of effective constituents are released. The ultra-fine green tea powder is added in the goat milk in proportion, and the obtained product not only has various nutrient substances and tea fragrance in green tea, but also has treatment effect of reducing high blood pressure, and has wide application prospect in the food industry. The tea powder is prepared by low-temperature ultra-fine pulverization, the original color, flavor and taste quality of tea leaves can be maintained effectively, after the tea leaves is pulverized into ultra-fine powder, the specific surface area of the tea powder is increased obviously, the effective constituents are released easily, human absorption can be improved, and therefore, nutrition absorptivity of human to the tea leaves is improved. Ultra-fine pulverization is a pure physical process, and pure natural property of processed products is maintained.