17 results about "Ace inhibition" patented technology

The invention discloses a screening method of hericium erinaceus functional polypeptide based on artificial intelligence assistance and polypeptide or a polypeptide composition. The method comprises the following steps: (1) constructing an AI auxiliary annotation model; (2) extracting hericium erinaceus crude protein; (3) preparing hericium erinaceus protein enzymatic hydrolysate through composite enzymatic hydrolysis; (4) performing polypeptide identification on the hericium erinaceus protein enzymatic hydrolysate to obtain a polypeptide sequence; and (5) carrying out AI auxiliary annotation and polypeptide screening by using a large language model based on a DeepSeek platform so as to obtain a polypeptide sequence with assumed biological activity. The polypeptide sequence and the polypeptide composition which have remarkable ACE inhibition and oxidation resistance dual functions are successfully obtained by utilizing the method disclosed by the invention. Compared with a traditional experience screening path, the method realizes a high-throughput active peptide development process which is targeted in structure, accurate in prediction and capable of verifying a closed loop.

An antihypertensive peptide probiotic goat milk powder and a preparation method thereof are provided. Goat milk is used as a raw material, the goat milk is sterilized and then cooled, and the screened probiotic bacteria for fermenting goat milk to produce antihypertensive peptides, namely Lacticaseibacillus rhamnosus KD5 (which is preserved in CCTCC on Sep. 7, 2023, with a preservation number of CCTCC NO: M20231641), is added and stirred evenly, and then fermented at a constant temperature. After the fermentation is completed, probiotic fermented goat milk containing antihypertensive peptides is obtained. After mixing the probiotic fermented goat milk evenly with or without nutritional fortifiers for middle-aged and elderly people, it is subjected to vacuum low-temperature spray-drying to obtain antihypertensive peptide probiotic goat milk powder, which has a high ACE inhibition rate and can be used to assist consumers in lowering blood pressure.

The invention relates to the technical field of bioengineering, in particular to a preparation process for extracting antihypertensive peptide from euphausia superba. The process comprises the following steps: adding a ferulic acid / arginine double-grafted pullulan compound in a preparation process, and breaking an emulsification interface by utilizing beta-cyclodextrin adsorption and phospholipase A2 cracking to obtain a soluble pretreatment product; meanwhile, short-time round-trip pH pulse is implemented in the incision enzyme hydrolysis process to activate potential enzyme cutting sites, finally, hydrophobic aggregation peptides are removed through weak acid rapid elution, and excision hydrolysis is performed to obtain a target product with the molecular weight of 0.5-3 kDa, so that dynamic induction and antioxidant steady-state dual control of hydrolysis reaction is realized; the generation proportion of short peptides and the ACE inhibition persistence are improved, and the flavor and the storage stability are remarkably improved. The method is controllable in technological process, high in repeatability and suitable for large-scale preparation of the euphausia superba protein functional peptide and development and application of antihypertensive food.

The invention relates to the technical field of functional food, in particular to a tartary buckwheat and roxburgh rose composition for regulating blood pressure and blood fat and a preparation method thereof. The tartary buckwheat and roxburgh rose composition comprises tartary buckwheat and roxburgh rose. The mass ratio of the tartary buckwheat to the roxburgh rose is 1: (1-3). In-vitro activity evaluation, cell experiments and animal experiments prove that flavonoid compounds of tartary buckwheat and active ingredients such as vitamin C of roxburgh rose in the composition generate a synergistic effect, so that the antioxidant capacity, the ACE inhibition rate, the cholesterol clearance rate and the pancreatic lipase inhibition rate can be remarkably improved, NO generation is effectively promoted, and the curative effect is good. The contents of TC, TG and LDL-L are reduced, the HDL-L level is increased, and the balance of vascular active substances is regulated. The raw materials are all medicinal and edible, are high in safety and free of side effects, provide a natural and efficient auxiliary conditioning scheme for chronic metabolic diseases such as hypertension and hyperlipidemia, and have a good industrialization prospect.

The invention relates to the technical field of biological medicine and food fermentation, in particular to a black fungus antihypertensive peptide culture medium composition based on selenium-enriched yeast protein, a liquid fermentation method and antihypertensive application of the black fungus antihypertensive peptide culture medium composition. The culture medium comprises selenium-enriched yeast protein, glucose, fermented soybean water extract dregs, inorganic salt and trace elements, the high-activity antihypertensive peptide is obtained by optimizing a liquid fermentation and separation purification process, the ACE inhibition IC50 value of the high-activity antihypertensive peptide is smaller than 50 micrograms per milliliter, and the high-activity antihypertensive peptide can be compounded with a hawthorn extract and the like to prepare an oral preparation and has a remarkable antihypertensive function.

The invention discloses a miichthys miiuy ACE inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of ACE inhibitory peptides. The ACE inhibitory peptide of the miichthys miiuy provided by the invention comprises an amino acid sequence as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5 or Asp-Gln-Gly. The ACE inhibitory peptide with the best activity is prepared and purified by taking miichthys miiuy meat as a raw material and taking the ACE inhibition rate as an index. The influence, antioxidant activity and mechanism of the ACE inhibitory peptide on blood pressure regulation factors are researched from the chemical level, the cellular level and computer simulation, the novel ACE inhibitory peptide is found, and a theoretical basis is provided for ocean resource high value.

The invention discloses a preparation method of a double-strain synergistic fermentation antihypertensive beverage, and particularly relates to the technical field of brewing beverage preparation. The double-strain synergistic fermentation antihypertensive beverage comprises the following raw materials in parts by weight: 1-2 parts of blue thistle pollen, 10-15 parts of a dogbane leaf decocted preparation, 5-15 parts of a pomegranate flower decocted preparation, 10-15 parts of a rose decocted preparation, 10 parts of a coreopsis bicolor decocted preparation, 2-5% of fructo-oligosaccharide, a viable count ratio of bifidobacterium longum 1714 to lactobacillus plantarum CW006 being (1-2): 1, and the balance of water. According to the preparation method of the double-strain synergistic fermentation antihypertensive beverage disclosed by the invention, four breakthroughs are realized through double-strain synergistic fermentation: the viable count reaches 1.2 * 10 < 8 > CFU / mL, and the ACE inhibition rate is 89.2%, so that the industry is new and high; the anthocyanin retention rate is 82%, the stability double guarantee is formed in the 45-day shelf life, and the centrifugal stability is 2.3% and is superior to the national standard by 5 times; the fermentation period is shortened to 14 hours, the yield reaches 98.7% in cooperation with real-time biosensing monitoring, the double-bacterium symbiotic index SI is equal to 1.32, and the yield of gamma-aminobutyric acid is increased by 61%.

This invention belongs to the field of food science and technology, specifically relating to a composition for cardiovascular protection and its preparation method. The composition includes freeze-dried natto powder, milk protein peptide powder, dihydromyricetin, and functional red yeast rice powder. The freeze-dried natto powder has a nattokinase content of 3000 FU / g, 5000 FU / g, 10000 FU / g, or 20000 FU / g. The milk protein peptide powder has a total VPP and IPP content ≥5.0%, and the milk protein peptide powder has an ACE inhibition rate ≥63%. This invention addresses the limitations of existing cardiovascular health foods that rely on single-component efficacy and cannot achieve multi-target, comprehensive protection. It employs a scientific combination of four natural active ingredients—freeze-dried natto powder, milk protein peptide powder, dihydromyricetin, and functional red yeast rice powder—to achieve synergistic effects across multiple targets.

The application discloses a plant extraction compound for reducing blood pressure, a preparation method and application thereof. The compound is composed of high-purity silybin extract and a specific fusion polypeptide through molecular self-assembly. The sequence of the fusion polypeptide is shown in sequence SEQ ID NO:1, which innovatively integrates cell membrane penetration function, angiotensin II receptor 1 antagonism function and hydrophobic drug binding function. The core of the application is that through polypeptide-mediated active targeting, silybin is accurately delivered to the diseased blood vessel area, and the double synergistic mechanism of upstream ACE inhibition and downstream receptor blocking of the renin-angiotensin-aldosterone system is realized. In-vivo pharmacodynamic experiments prove that compared with single components, the compound can produce more significant and persistent antihypertensive effect, and can effectively reverse myocardial hypertrophy and fibrosis caused by hypertension, and show excellent synergistic therapeutic effect and target organ protection ability.

The invention discloses a sophora moorcroftiana protein source antihypertensive peptide and application of the sophora moorcroftiana protein source antihypertensive peptide. The sophora moorcroftiana protein source antihypertensive peptide comprises at least one of active peptides with the sequence structure of MGLPWYR, WHRPLR, INPLGFR, LPNSLFLFR and LFLWPIYR. A polypeptide sequence library is constructed through proteolysis of sophora moorcroftiana, bioinformatics analysis is combined, multiple polypeptides with ACE inhibitory activity are found, after high-purity polypeptides are obtained through a chemical method, a liquid chromatograph-mass spectrometer is used for characterization, the effect of the polypeptides is verified through ACE inhibition experiments, all the polypeptides can inhibit ACE, and the inhibitory activity of the polypeptides MGLPWYR and WHRPLR is about 90%.

The invention relates to yak plasma protein peptide powder with strong antioxidation and ACE (angiotensin converting enzyme) inhibition functions and a preparation method thereof, and the preparation method comprises the following steps: S1, dissolving yak plasma protein powder in water, sterilizing at high temperature, cooling and homogenizing to obtain yak plasma protein liquid; s2, performing enzymolysis on the yak plasma protein liquid to obtain yak plasma protein enzymatic hydrolysate; s3, sequentially performing multi-stage separation, concentration and drying on the yak plasma protein enzymatic hydrolysate to obtain yak plasma protein peptide powder with strong antioxidation and ACE inhibition functions; and S4, determining the components of the yak plasma protein peptide powder. The amino acid sequence EDGGGWWYNR of the yak plasma protein peptide powder is 39.5%-42.5%, and the amino acid sequence WYNR of the yak plasma protein peptide powder is 15.3%-17.8%. The yak plasma protein peptide powder disclosed by the invention has remarkable strong anti-oxidation and ACE (Angiotensin Converting Enzyme) inhibition effects, and is good in water solubility, easy to absorb by a human body and free from unpleasant odor. The preparation method is easy to operate and suitable for industrial production, acid and alkali are not added in the whole preparation process, and green processing can be achieved.

The application relates to the technical field of food processing, and discloses a preparation method of celery juice with high retention rate of active ingredients, which comprises the following steps: pretreating celery to obtain pretreated celery sections; mixing the celery sections with a composite enzyme to obtain a mixture, and then performing beating treatment to obtain celery slurry; performing enzymolysis treatment on the celery slurry to obtain celery slurry after enzymolysis; performing enzyme inactivation treatment on the celery slurry after enzymolysis, and then performing centrifugation to obtain primary celery juice; performing ultra-high temperature instant sterilization on the primary celery juice, and then performing sterile filling to obtain celery juice products. The celery juice obtained by the combined beating treatment has improved DPPH free radical scavenging rate, enhanced antioxidant activity and improved ACE inhibition rate. The activity of the celery juice is not only derived from flavonoids, but also derived from ACE inhibitory peptides newly generated by neutral protease hydrolysis of celery protein, and the two contribute to each other, so that the blood pressure lowering function is obviously better than that of the celery juice without enzyme or the celery juice prepared by a single beating process.

The invention discloses a buffalo milk active polypeptide preparation method, and relates to the technical field of dairy product processing, and the buffalo milk active polypeptide preparation method comprises the following steps: preparing buffalo cheese protein into a substrate solution; performing high-pressure homogenization and mild heat treatment on the substrate solution; carrying out two-stage enzymolysis on the pretreated substrate solution, wherein the first-stage enzymolysis adopts an enzyme combination mainly comprising incision enzyme, and the hydrolysis degree is controlled to be 10-22%; in the second-stage enzymolysis, flavourzyme and aminopeptidase or a compound enzyme preparation containing flavourzyme and aminopeptidase are added into the reaction system, and enzymolysis is carried out until the total hydrolysis degree reaches 28%-40%; separating the enzymatic hydrolysate by adopting an ultrafiltration membrane and a nanofiltration membrane, and collecting permeate with the molecular weight of less than 2000Da; and carrying out post-treatment on the permeate to obtain the buffalo milk active polypeptide. According to the method, the bitter taste of the product can be remarkably reduced, and specific sequences with high-value biological activities such as ACE inhibition and oxidation resistance can be better reserved and enriched.

The invention discloses a polypeptide with effects of reducing blood pressure and resisting oxidation as well as a related product and application thereof, and relates to the technical field of biology. The polypeptide with angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained through screening, and the amino acid sequence of the polypeptide is AVPYPQRDMP. In-vitro activity evaluation results show that the polypeptide not only has strong ACE inhibitory activity, but also can effectively scavenge free radicals; the ACE inhibition IC50 value is 258.46 mu M, the free radical scavenging TEAC value is 1.47 mu mol TE / mu mol, and compared with similar polypeptides, the polypeptide has better ACE inhibition effect and free radical scavenging effect. In-vivo antihypertensive evaluation is carried out through a spontaneous hypertension rat model, it is also shown that the compound has a good antihypertensive effect, and the compound can be used for preparing products related to antihypertensive and / or antioxidation.

The invention provides a preparation method of a comprehensive tea source active peptide with controllable activity. The method can be used for preparing the comprehensive tea source active peptide with the main ACE inhibition activity, such as ACE inhibition ratio gt; 45% of a comprehensive tea source ACE inhibitory peptide; the comprehensive tea source active peptide with antioxidant activity as a main component can be prepared, for example, the DPPH clearance rate is gt; 40% of a comprehensive tea source antioxidant peptide; and the preparation process is simple and controllable, and batch continuous preparation can be realized.

An antihypertensive peptide probiotic goat milk powder and a preparation method thereof are provided. Goat milk is used as a raw material, the goat milk is sterilized and then cooled, and the screened probiotic bacteria for fermenting goat milk to produce antihypertensive peptides, namely Lacticaseibacillus rhamnosus KD5 (which is preserved in CCTCC on Sep. 7, 2023, with a preservation number of CCTCC NO: M20231641), is added and stirred evenly, and then fermented at a constant temperature. After the fermentation is completed, probiotic fermented goat milk containing antihypertensive peptides is obtained. After mixing the probiotic fermented goat milk evenly with or without nutritional fortifiers for middle-aged and elderly people, it is subjected to vacuum low-temperature spray-drying to obtain antihypertensive peptide probiotic goat milk powder, which has a high ACE inhibition rate and can be used to assist consumers in lowering blood pressure.

The invention discloses a hexapeptide NS6 with ACE inhibitory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the hexapeptide NS6 is NLLELS, and the hexapeptide NS6 can be prepared through a solid-phase synthesis method and an enzymolysis method. The hexapeptide NS6 is identified from polysiphonophyta protein enzymatic hydrolysate and has potential interaction with ACE, under the concentration of 0.1 mg / mL, the ACE inhibition rate is 82.74%, and compared with VPP and IPP (under the same concentration, the ACE inhibition rates are 82.32% and 78.00% respectively), the ACE inhibition activity is not superior to that of VPP, but is remarkably superior to that of IPP (plt; 0.05), richer choices are provided for development of auxiliary blood pressure lowering functional food needing precise formula design within a specific activity range, and the hexapeptide NS6 can be used for preparing blood pressure lowering drugs or auxiliary blood pressure lowering functional food.