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Method for preparing wheat germ protein ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling

An ultrafiltration separation and wheat germ protein technology is applied in the field of continuous enzymatic hydrolysis and ultrafiltration separation coupling technology to prepare wheat germ protein ACE inhibitory peptides, which can solve the problems of reducing product activity, excessive product degradation, and complicated purification methods, etc. Improve utilization, avoid enzyme inactivation, and achieve continuous effects

Active Publication Date: 2009-08-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing research on wheat germ ACE inhibitory peptides is mainly through intermittent enzymatic hydrolysis, followed by downstream separation. The intermittent enzymatic hydrolysis method has the following important defects: low enzymatic hydrolysis efficiency, low utilization rate of unit enzyme resources; excessive degradation of products, Reduced product activity; discontinuous process and cumbersome purification methods
These problems have seriously restricted the production and marketization process of this product

Method used

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  • Method for preparing wheat germ protein ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling
  • Method for preparing wheat germ protein ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Add defatted wheat germ powder into water at a concentration of 10% (w / v), stir at a pH value of 9.5 for 0.5 h, centrifuge to obtain a supernatant to adjust the pH value to 7.0, and use a medium-temperature amylase at 70° C. for 180 min to enzymatically hydrolyze. The amount of enzyme added is 3% (w / v); after enzymatically degrading the starch, adjust the pH to 4.5 to obtain a precipitate, dissolve and dilute with water, adjust the pH to neutral, and spray dry.

[0015] Weigh 5kg of wheat germ protein, add 50 times the weight of deionized water, stir and dissolve, configure a 2% solution, add 8% (w / w) alkaline protease of the wheat germ protein quality after homogenization, and maintain the reaction solution The pH value is 9.0, and the enzymatic hydrolysis reaction is carried out at 40°C. The enzymatic hydrolysis reaction solution is sent to the ultrafiltration device, the product solution passes through the ultrafiltration membrane, and the unreacted substrate and enz...

Embodiment 2

[0017] Add 8% (w / v) of defatted wheat germ powder into water, stir at pH 8.5 for 0.5 h, centrifuge to obtain the supernatant, adjust the pH to 6.5, use medium-temperature amylase at 60°C for 240 min, add The enzyme amount is 1% (w / v); after enzymatically degrading the starch, adjust the pH to 4 to obtain a precipitate, dissolve and dilute with water, adjust the pH value to neutral, and spray dry.

[0018] Take 5kg wheat germ protein, add 100 times of weight deionized water, stir and dissolve, configure 1% wheat germ solution, add alkaline protease after homogenization, the enzyme amount is 6% (w / w), maintaining the pH value of the reaction solution at 8.0, and performing the enzyme-membrane coupling reaction at 50°C. The enzymatic hydrolysis reaction solution is sent to the ultrafiltration device, the product solution passes through the ultrafiltration membrane, and the unreacted substrate and enzyme solution continue to return to the reactor for reaction. The molecular weigh...

Embodiment 3

[0020] Add defatted wheat germ powder at a concentration of 12% (w / v) to water, stir at pH 9.5 for 0.5 h, centrifuge to obtain the supernatant, adjust the pH to 7.5, use medium-temperature amylase at 70°C for 120 min, add The enzyme amount is 2.5% (w / v); after enzymatically degrading the starch, adjust the pH to 4 to obtain a precipitate, dissolve and dilute with water, adjust the pH to neutral, and spray dry.

[0021] Weigh 5kg of wheat germ protein, add 100 times the weight of deionized water, stir to dissolve, configure 1% wheat germ solution, add neutral protease after homogenization, the amount of enzyme added is 4% of the mass of wheat germ protein, and maintain the reaction The pH value of the solution was 7.0, and the enzyme-membrane coupling reaction was carried out at 45°C. The enzymatic hydrolysis reaction solution is sent to the ultrafiltration device, the product solution passes through the ultrafiltration membrane, and the unreacted substrate and enzyme solution ...

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Abstract

The invention relates to a method for preparing wheat germ protein angiotensin-converting enzyme ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling; the method is carried out according to the following steps: degreasing wheat embryo powder is crashed, and the wheat germ protein is extracted by a middle-temperature amylase method; after the wheat germ protein solution is homogenized, the preparation and separation of wheat germ antihypertensive peptide is carried out by adopting the technology of protein continuous enzymolysis and the ultra-filtration separation coupling, finally, the powdered wheat germ antihypertensive peptide is obtained by compression and drying. The method can lead the product to be on-line separated so as to avoid excessive enzymolysis; meanwhile, the enzyme is utilized in a recycling way and the utilization rate of unit enzyme is improved; in addition, enzyme eliminating operation is avoided by interval operation, the continuity of the process for preparing the wheat germ protein ACE inhibitory peptide is realized. The wheat germ protein ACE inhibitory peptide which is obtained by the method can be used for preparing medicines or health care foods with the function of inhibiting the activity of the internal angiotensin-converting enzyme ACE.

Description

Technical field: [0001] The invention relates to the field of bioengineering, in particular to the field of functional food production technology and biopharmaceuticals, in particular to a method for preparing wheat germ protein ACE inhibitory peptide by coupling continuous enzymolysis and ultrafiltration separation. Background technique: [0002] Angiotensin-converting enzyme (ACE) plays an important role in regulating blood pressure and cardiovascular function. ACE inhibitors prevent the conversion of angiotensin I to angiotensin II, which has a strong vasoconstrictive effect, by inhibiting ACE, and at the same time prevent ACE from degrading bradykinin, which has a vasodilation effect, so as to achieve the purpose of lowering blood pressure. [0003] Wheat germ is the main by-product of flour processing, and it is also the most nutritious part of wheat. Its content is about 2%-3% in wheat grain, and it contains extremely rich high-quality protein, fat, vitamins, starch an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/01A61P9/12A23L1/305A23L33/18
Inventor 马海乐王振斌骆琳何荣海赵伟睿
Owner JIANGSU UNIV
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