Method for preparing wheat germ protein ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling

An ultrafiltration separation and wheat germ protein technology is applied in the field of continuous enzymatic hydrolysis and ultrafiltration separation coupling technology to prepare wheat germ protein ACE inhibitory peptides, which can solve the problems of reducing product activity, excessive product degradation, and complicated purification methods, etc. Improve utilization, avoid enzyme inactivation, and achieve continuous effects
CN101514355AActive Publication Date: 2009-08-26JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2009-08-26

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Abstract

The invention relates to a method for preparing wheat germ protein angiotensin-converting enzyme ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling; the method is carried out according to the following steps: degreasing wheat embryo powder is crashed, and the wheat germ protein is extracted by a middle-temperature amylase method; after the wheat germ protein solution is homogenized, the preparation and separation of wheat germ antihypertensive peptide is carried out by adopting the technology of protein continuous enzymolysis and the ultra-filtration separation coupling, finally, the powdered wheat germ antihypertensive peptide is obtained by compression and drying. The method can lead the product to be on-line separated so as to avoid excessive enzymolysis; meanwhile, the enzyme is utilized in a recycling way and the utilization rate of unit enzyme is improved; in addition, enzyme eliminating operation is avoided by interval operation, the continuity of the process for preparing the wheat germ protein ACE inhibitory peptide is realized. The wheat germ protein ACE inhibitory peptide which is obtained by the method can be used for preparing medicines or health care foods with the function of inhibiting the activity of the internal angiotensin-converting enzyme ACE.
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Description

Technical field:

[0001] The invention relates to the field of bioengineering, in particular to the field of functional food production technology and biopharmaceuticals, in particular to a method for preparing wheat germ protein ACE inhibitory peptide by coupling continuous enzymolysis and ultrafiltration separation. Background technique:

[0002] Angiotensin-converting enzyme (ACE) plays an important role in regulating blood pressure and cardiovascular function. ACE inhibitors prevent the conversion of angiotensin I to angiotensin II, which has a strong vasoconstrictive effect, by inhibiting ACE, and at the same time prevent ACE from degrading bradykinin, which has a vasodilation effect, so as to achieve the purpose of lowering blood pressure.

[0003] Wheat germ is the main by-product of flour processing, and it is also the most nutritious part of wheat. Its content is about 2%-3% in wheat grain, and it contains extremely rich high-quality protein, fat, vitamins, starch an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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