341 results about "Wheat grain" patented technology

The invention discloses a wheat single grain appearance anomaly detection method based on a deep learning model and hyperspectral imaging. The method comprises the following steps: step 1, collecting different types of wheat grain samples; step 2, acquiring hyperspectral image data of the wheat grains by using a visible light-near infrared and short wave near infrared hyperspectral imaging system, and extracting spectral information and image information; step 3, preprocessing the spectral data, and verifying a preprocessing effect; 4, screening spectral characteristic wave bands, extracting texture characteristics and morphological characteristics in combination with a gray-level co-occurrence matrix, and constructing a middle-level data fusion model; step 5, constructing an atlas feature fusion deep learning model to realize high-level fusion of spectrum and image features; and step 6, pixel-level classification is carried out on the hyperspectral image, and a spatial distribution visualization result of the appearance abnormity of the wheat grains is generated. The method has high precision, nondestructive testing and strong generalization ability, and is suitable for wheat quality grading, processing and sorting and storage safety management.

Provided is the use of a protein derived from wheat and a biomaterial related thereto in increasing the yield per plant and grain protein content of a crop. The protein is designated as SWEET11, and may be a protein having an amino acid sequence as shown in sequence 9, sequence 6 or sequence 3 in the sequence listing. The wheat SWEET11 protein (TaSWEET11) is over-expressed in the Zhengmai 7698 wheat variety (nationally approved), and it is found that the over-expression of TaSWEET11 can significantly improve the thousand-grain weight of wheat grains and increase the yield. In addition, the over-expression of TaSWEET11 also increases the protein content in the wheat grain, significantly improving the grain quality while increasing the yield. The TaSWEET11 protein can be used for breeding and quality improvement of crops.

The invention discloses a wheat grain protein spatial distribution difference identification method. The method comprises the following steps: S1, constructing a semantic segmentation data set; an improved U-Net network is adopted as a model architecture, and preliminary background removal is completed; s2, binarization processing is carried out, and preliminary segmentation is completed; obtaining an endosperm edge contour by using a Sobel edge detection algorithm; determining the center position of an endosperm area; carrying out layer-by-layer corrosion to obtain a radial layered structure image; s3, acquiring a sampling matrix; a K-means + + algorithm is adopted to initialize the clustering center for iteration; and identifying the protein and calculating the space distribution proportion of the protein. According to the method, semantic segmentation of the U-Net model, dynamic layering of radial corrosion and component clustering of the K-means + + algorithm are improved, full automation from image preprocessing to protein quantitative analysis is achieved, tedious operation of manual description is reduced, image processing is more accurate, layering processing can be conducted on the image, and the protein distribution gradient can be accurately reflected.

The invention relates to the technical field of genetic engineering, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to the protein content of wheat grains and application. The SNP molecular marker contains a nucleotide sequence of which the polymorphism is T or C at the 167440648 bp position of a wheat 7B chromosome. According to the present invention, the research determines that the site is the SNP site closely related to the protein content, and the specific KASP primer combination designed based on the SNP site can rapidly and accurately identify the wheat protein content. According to the technical scheme provided by the invention, the selection efficiency can be improved, the breeding period can be shortened, and wheat varieties with ideal protein content can be quickly identified and cultivated. The KASP primer combination provided by the invention provides an effective tool for molecular marker-assisted selection of wheat nutritional quality related traits.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention discloses a wheat yield remote sensing prediction method combining phenological parameters, which comprises the following steps: S1, performing field observation in a key growth period of winter wheat, synchronously collecting canopy hyperspectral reflectivity data, leaf area index (LAI) and SPAD value of each observation sample point, and recording wheat grain yield of the corresponding sample point; s2, performing noise reduction preprocessing on the acquired canopy hyperspectral data, and extracting sensitive spectral parameters by combining principal component analysis (PCA) and correlation analysis methods; s3, taking the sensitive spectrum parameters, LAI and SPAD values as independent variables, taking the wheat grain yield as a dependent variable, and adopting partial least squares regression (PLSR) to construct a multivariable yield prediction model; and S4, performing wheat yield prediction on an independent test sample or regional scale remote sensing data by using the trained model. The method overcomes the defect that only yield sensitive spectrum parameters are used for predicting the effect, and accurate estimation of the model is achieved.

The invention discloses a hyperspectrum-based wheat variety identification method. The method comprises the following steps: establishing a hyperspectrum identification model set of a target variety; checking and screening to obtain a target variety hyperspectral recognition model; and detecting the purity of the to-be-detected wheat variety in the wheat blind sample by using the variety identification hyperspectral model. And constructing a hyperspectral recognition model set of the target variety, wherein the modeling process comprises the following steps: confirming a wheat grain sample area in a spectrum acquisition process, extracting an average spectrum of a wheat grain modeling sample and constructing a recognition model. Screening and optimization of a target variety hyperspectral recognition model: selecting a to-be-detected sample with known target variety purity; determining a wheat grain sample area during sample spectrum collection; identifying a target variety in the to-be-detected wheat grain sample, calculating the purity of the target variety, and comparing the purity of the target variety with a true purity value of the target variety to obtain a hyperspectral identification model of the wheat target variety for identifying the target variety.

The present invention relates to the field of wheat breeding, and specifically relates to a TaMyb10-94E gene related to grain color and pre-harvest sprouting resistance and the use thereof. The TaMyb10-94E gene was discovered on a 3D chromosome of Suining Tuotuo wheat, and the function of the TaMyb10-94E gene in the aspect of regulating the grain color and pre-harvest sprouting resistance of wheat has been verified, indicating that the special mutation can confer pre-harvest sprouting resistance to wheat without producing red grains. Additionally, with regard to the mutation, a method for identifying the TaMyb10-94E gene based on an SNP site is further developed, which is beneficial for the efficient breeding of white-grain wheat varieties that are resistant to pre-harvest sprouting.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker closely linked with the peel thickness of wheat grains and application of the SNP marker, and belongs to the technical field of biology, an SNP site closely linked with the peel thickness character of the wheat grains is excavated through genome-wide association analysis, the SNP site is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat, and the SNP site is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat and is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat. The site base has A / G polymorphism, and the AA genotype or AG genotype has higher grain peel thickness than the GG genotype. Through genotype detection of the SNP site, the wheat grain pericarp thickness character can be obviously distinguished, a KASP primer is developed based on the SNP site, and molecular marker-assisted identification or assisted breeding can be performed on the wheat grain pericarp thickness character.

The utility model relates to the technical field of wheat detection, and discloses an imperfect wheat grain detection system which comprises a base, and a mounting frame is arranged on the base; the vibration feeding disc is arranged on the base and is used for orderly conveying the wheat to the discharge port one by one; and the high-speed cameras are located below the discharging port of the vibration feeding disc, the multiple high-speed cameras are arranged in a circle, and a wheat discharging channel is defined by the multiple high-speed cameras. Compared with a traditional scheme, wheat samples do not need to be arranged, meanwhile, the multiple high-speed cameras are arranged at the discharging port of the vibration feeding disc, when wheat grains pass through the wheat discharging channel defined by the high-speed cameras, the multiple high-speed cameras conduct 360-degree photographing detection on the wheat grains at the same time, and therefore the wheat grains can be accurately detected. The detection range is more comprehensive, and the detection result is more accurate.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a TaMGD3 gene in improvement of plant starch quality and cultivation of a high-starch transgenic plant, including application of the TaMGD3 gene in promotion of wheat grain starch synthesis, application of the TaMGD3 gene in cultivation of the high-starch and / or high-amylopectin transgenic plant, and application of the TaMGD3 gene in improvement of plant starch quality and cultivation of the high-starch and / or high-amylopectin transgenic plant. The invention also discloses an application of the TaMGD3 gene in improvement of plant starch characteristics. A nucleotide sequence of the TaMGD3 gene is shown as SEQ ID NO. 1. The invention also provides a cultivation method of transgenic wheat. In wheat cultivation, wheat amylopectin quality improvement is realized through overexpression of the gene shown in SEQ ID NO.1. The TaMGD3 gene can improve the starch content of wheat grains, influence the particle size distribution of the wheat grain starch, improve the amylopectin proportion, improve the peak viscosity and the relative crystallinity of the starch, and facilitate the improvement of the cooking quality related to the wheat starch.

The invention discloses an application of a TaAP2-4 gene in regulating and controlling starch content of wheat grains, which is used for enhancing the expression quantity of the TaAP2-4 gene to improve the starch content of the wheat grains and weakening the expression quantity of the TaAP2-4 gene to reduce the starch content of the wheat grains. The TaAP2-4 gene is located on a chromosome 4 of a wheat genome, and three copies, namely TaAP2-4-A, TaAP2-4-B and TaAP2-4-D, are formed on the chromosome 4. According to the invention, a Crispr / Cas9 gene editing vector for knocking out the TaAP2-4 gene is constructed by adopting a reverse genetics method, the TaAP2-4 gene is successfully knocked out in a wheat material by adopting an agrobacterium-mediated method, and the starch content of a knocked-out mutant is reduced, so that the condition that the TaAP2-4 gene plays a positive regulation role in a wheat grain starch synthesis process is known. In practical application, directional knockout mutation of the genome level TaAP2-4 gene can be achieved through a gene transformation process, the gene knockout mutation method can be used for verifying functions of the TaAP2-4 gene, and materials are provided for mining other starch synthesis related genes.

The invention discloses a plant source leaf surface resistance and control agent for efficiently reducing the cadmium content of wheat and a preparation method thereof. According to the invention, S-allyl-L-cysteine is used as an important component of the foliar blocking and controlling agent, so that the bioavailability of cadmium ions is effectively reduced, and the absorption and transport of cadmium in plants are reduced, thereby reducing the cadmium content in wheat grains. Meanwhile, S-allyl-L-cysteine has relatively strong antioxidant capacity, so that free radicals in the plant body can be removed, and cells are protected from oxidative damage. As a plant growth regulator, S-allyl-L-cysteine can improve nutrient absorption and utilization of plants and promote photosynthesis and energy metabolism of the plants, so that the yield and quality of wheat are improved. The S-allyl-L-cysteine can activate a defense mechanism in a plant body, enhance the resistance of the plant to environmental stress, including the resistance to heavy metal pollution, and improve the overall health and yield of wheat.

The invention discloses a polyploidy breeding method for wheat plant genome doubling, which belongs to the technical field of plant breeding, and comprises the following steps: treating germinated wheat for 30 days under the conditions of low temperature of 2-7 DEG C and 24-hour illumination, then transplanting into soil, culturing for 45-55 days under the conditions that the temperature is 20-25 DEG C, the light-dark culture period is 20-22 hours, and the light-dark culture period is 2-4 hours, the illumination intensity is 10000-20000 lx, and the culture time is 20-30 days. Wherein the ratio of the red light to the blue light is 3: 2, obtaining a large number of chromosome group doubled seeds until the wheat grains are mature, and realizing wheat plant chromosome group doubling. Experiments prove that the method is easy to operate, toxic reagents are avoided, the period is short, and the efficiency of obtaining wheat with doubled chromosome groups is high.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a plant growth regulator for inhibiting plant germination and application thereof, the plant growth regulator for inhibiting plant germination is obtained by compounding S-abscisic acid and dihydrogibberellin; in the plant growth regulator for inhibiting plant germination, the molar ratio of dihydrogibberellin to S-abscisic acid is (0.2-2): (0.1-1). By combining dihydrogibberellin with S-abscisic acid, the proportion of endogenous hormone gibberellin and abscisic acid in wheat grains can be effectively reduced, the activity of alpha-amylase is reduced, the content of soluble sugar is reduced, the dormancy capacity of the wheat grains is enhanced, and the germination rate of the wheat grains in the mature period is remarkably reduced.

The invention discloses a wheat grain moisture content lossless prediction method based on hyperspectral imaging and Wasserstein generative adversarial network data enhancement, which realizes accurate moisture prediction by fusing spectral feature data enhancement and deep learning modeling. Collecting spectral image data of the wheat grains by using a visible light-near infrared and short wave infrared hyperspectral imaging system; a Wasserstein generative adversarial network is adopted to generate synthetic spectrum and moisture data in a differentiated mode, data distribution consistency is verified through t-SNE, and a triple training set is expanded; noise is eliminated in combination with first-order derivative preprocessing, key characteristic wavelengths are screened by using SPA and ReliefF algorithms, and a convolutional neural network regression model is constructed; experiments show that the method achieves # imgabs0 # imgabs1 # RMSEP = 0.5717 in the visible light-near infrared band and # imgabs2 # RMSEP = 1.0009 in the short wave infrared band, and the precision is remarkably improved compared with a traditional extreme learning machine and a back propagation neural network.

The invention relates to a method for regulating and controlling the color of wheat grains and reducing the germination rate of the grains, a related TaMyb10 mutant protein, a gene, a gene editing vector and application of the TaMyb10 mutant protein. The invention discloses a method for regulating and controlling the color of wheat grains and reducing the germination rate of the grains. The method comprises the step of inhibiting or destroying the interaction of TaMyb10 protein and bHLH protein in wheat to obtain white-grain pre-harvest germination resistant wheat. The invention further discloses a functional segment which is combined with bHLH in the TaMyb10 protein and can influence the color and the pre-harvest germination resistance of wheat grains, application of a variant of the functional segment, TaMyb10 mutant protein containing substitution and / or deletion and / or addition of amino acid residues at one or more functional sites of the functional segment, and application of the TaMyb10 mutant protein. The interaction between the TaMyb10 and the bHLH protein is weakened or does not interact with the bHLH protein through the mutation of the amino acid at the functional site, so that the wheat shows the characteristic of white-grain pre-harvest sprouting resistance, and the cultivation of the white-grain pre-harvest sprouting resistance wheat variety is facilitated.

The present invention discloses an air impact separation type cereal processing mechanism, including a processing box body, a floating pressing mechanism, a bottom pressing plate, and an air impact mechanism; in the present invention, when the diversion ring cover moves downward and abuts against the upper periphery of the pressing groove of the bottom pressing plate, the pressing column continues to move downward and is inserted into the pressing groove on the upper side of the bottom pressing plate, so that the wheat grains in the pressing groove are pressed into the shape of cereal. Then, the floating box drives the pressing column to move upward. When the pressing column moves upward out of the pressing groove and moves into the inside of the diversion ring cover and the diversion folding ring, air is inhaled from the outside through the suction pipe. The air enters the floating box through the telescopic pipe, and after passing through the annular air cavity, it is discharged from the horizontal diversion ring groove. In this way, the cereal attached to the bottom of the pressing column is separated by the air flow in the horizontal direction and falls into the original pressing groove, thus greatly improving the convenience and efficiency of separating the cereal attached to the bottom of the pressing column.

The utility model discloses a kernel moxibustion device, belongs to the technical field of medical tools, and provides the kernel moxibustion device capable of effectively reducing the scald risk and improving the smoke utilization rate. The kernel moxibustion device comprises an upper cover and a base, the base is provided with a positioning sleeve, the main body part of the positioning sleeve is a configuration ring, and a material disc is arranged in the configuration ring and suitable for containing moxa; the material disc is provided with convection holes penetrating through the upper surface and the lower surface, the configuration ring is provided with a smoke outlet penetrating through the inner wall and the outer wall below the material disc, and the upper cover is suitable for being buckled at the upper end of the positioning sleeve. By arranging the upper cover structure, smoke generated by moxa combustion is prevented from escaping upwards, the smoke falls through the convection holes of the material disc and makes full contact with the skin, the utilization rate of the smoke is effectively increased, the consumption of moxa is reduced, and the economical efficiency of kernel moxibustion is improved; due to the design of the base structure, moxa is burnt on the material disc with the holes, a heat source is prevented from being too close to the skin, the moxa does not need to be extinguished in time, and the scalding risk can be effectively reduced.

The application relates to the field of crop seeding technology, and particularly discloses a wheat-corn interplanting self-adaptive seeding width regulation method based on crop and soil parameter identification, which comprises the following steps: normalizing soil body penetration resistance data to generate soil body penetration resistance score values and dividing seeding condition grades; jointly analyzing wheat grain moisture content data and soil slope data to construct a slope moisture content correlation matrix and perform dimension expansion to obtain interference characteristic factors; inputting wheat row spacing data, the seeding condition grades and the interference characteristic factors into a decision rule library to generate seeding width values and seeding density values; converting the seeding width values into channel boundary coordinate instructions of seeding machines, and driving the seeding machines to adjust the operation width of corn seeding according to the channel boundary coordinate instructions; and synchronously regulating the single irrigation amount of wheat medium-shallow buried drip irrigation. The application can improve the seeding precision and resource utilization efficiency of wheat-corn interplanting operation.

The invention provides a wheat imperfect grain recognition method and a wheat imperfect grain recognition device. The method comprises the following steps: enabling wheat in a wheat sample to be detected to drop one by one; in the falling process of each wheat grain, at least two cameras are adopted to shoot each wheat grain so as to at least obtain images on the two sides of each wheat grain, and at least two images to be recognized are obtained; identifying the at least two to-be-identified images based on a constructed deep learning network model suitable for wheat imperfect grain images to obtain the grain type of each grain of wheat; and sorting each wheat grain to a corresponding collection area based on the grain type. According to the scheme, the recognition speed and the recognition precision of the wheat imperfect grains can be improved, the required calculation amount is small, and the method can be deployed in a resource-limited production environment.

The invention discloses a method for improving the nutritional quality of wheat and reducing vomitoxin by combining cold plasma with lactic acid bacteria fermentation. The method comprises the following specific steps: (1) putting wheat grains polluted by DON into a reaction cavity of cold plasma equipment; (2) preparing a single or compound lactic acid bacteria starter; (3) mixing the wheat subjected to cold plasma treatment with a lactic acid bacteria leavening agent for fermentation; and (4) drying, grinding and sieving the fermented wheat to prepare the wheat flour meeting the standard. Through progressive cooperative treatment of cold plasma pretreatment and compound lactobacillus fermentation, the final degradation rate of DON reaches 91.04%, functional components (resistant starch reaches 13.71% and dietary fiber reaches 13.14%) are synchronously increased, and phytic acid (lowest to 0.42%) is greatly degraded to improve the bioavailability of mineral substances. Besides, the method can optimize the wheat processing performance, the prepared noodles are moderate in hardness, good in elasticity and stretch-proof, no chemical reagent is added in the whole process, collaborative optimization of efficient detoxification, nutrient enrichment and processing performance improvement is achieved, and a green and economical solution is provided for safe utilization of polluted wheat.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

This invention discloses a haplotype SNP molecular marker for identifying wheat grain starch content in the TaMYB44-4D gene and its application. Specifically, the SNP is T or C at position 1557bp of the sequence in SEQ ID NO.1. Based on this SNP, primers for detecting this haplotype SNP molecular marker have also been developed, and a kit containing these primers has been prepared. By PCR and polyacrylamide gel electrophoresis, the SNP and genotype can be accurately identified, thereby distinguishing between superior haplotypes (high starch content) and non-superior haplotypes (low starch content) in wheat.

The utility model relates to the technical field of wheat processing, and discloses a wheat impurity screening device which comprises a screening frame, a screening plate, a fixing assembly and an elastic assembly, a vibration motor is fixedly installed on the outer side of the screening frame, the screening frame is arranged above a base, and an opening structure is arranged on the upper surface of the base. The wheat screening device has the following advantages and effects that wheat grains are daily screened through the screen mesh, when the screen mesh needs to be cleaned, the insertion rods can be driven by the driving assembly to move, so that the insertion rods can be moved out of the locking grooves, the plate body can be separated from the screening frame, the plate body is inclined, and the screening efficiency is improved. And meanwhile, after the plate body moves downwards, the sliding rods are aligned with the pin holes, so that the plate body can be connected with the sliding rods in an inserted mode, the plate body can be subjected to vibration force application of the vibration motor, the impurities can be conveniently shaken out in a vibration mode, and the impurities can be comprehensively and effectively cleaned. Therefore, the cleaning convenience and efficiency are improved.

The application provides a wheat kernel hardness major locus and a SNP marker Marker288063 and an application, belongs to the wheat breeding technical field, and mainly provides the SNP molecular markers Marker288063, Marker288023, Marker287945 and Marker288351; the SNP locus provided by the application has important significance for detecting and breeding a wheat kernel high HI variety, strain and breeding material, and a molecular marker can be developed according to the SNP locus, so that the wheat quality breeding efficiency is accelerated.

The invention relates to a method for increasing folic acid content of wheat malt seedlings through ultrasonic field and amino acid solution treatment, which belongs to the technical field of agricultural food biology and is characterized in that wheat grains with high germination rate are used as raw materials, and the wheat malt seedlings are cultivated through soaking disinfection, amino acid solution dipping and ultrasonic field treatment and regulation and control of illumination, temperature, humidity and CO2 concentration. And drying and crushing to obtain the wheat malt seedling powder rich in folic acid. According to the method disclosed by the invention, the permeability of cell membranes is enhanced by utilizing an ultrasonic cavitation effect, folic acid precursor absorption and synthetase activation are promoted, the folic acid content in the wheat malt seedlings is effectively increased, and the wheat malt seedling powder with the folic acid content of 450-550mu g / 100g is obtained.

The invention belongs to the technical field of gene engineering, and particularly relates to application of TaNAS1 protein or a coding gene thereof in regulation and control of plant nicotinamide content. The invention provides application of a TaNAS1 protein or a coding gene thereof in regulating and controlling the content of nicotinamide in plants. The amino acid sequence of the TaNAS1 protein is as shown in SEQ ID NO: 3. The TaNAS1 protein or the coding gene thereof is overexpressed in a plant, so that the nicotinamide content of the plant can be increased, the agronomic traits of wheat can be improved, and the nutrient element content of wheat grains can be increased. Specifically, overexpression of the TaNAS1 protein or the coding gene of the TaNAS1 protein increases the grain length, grain width and hundred-grain weight of wheat, and increases the contents of zinc, iron and copper elements in wheat grains.