140 results about "Wheat grain" patented technology

The invention discloses a wheat yield remote sensing prediction method combining phenological parameters, which comprises the following steps: S1, performing field observation in a key growth period of winter wheat, synchronously collecting canopy hyperspectral reflectivity data, leaf area index (LAI) and SPAD value of each observation sample point, and recording wheat grain yield of the corresponding sample point; s2, performing noise reduction preprocessing on the acquired canopy hyperspectral data, and extracting sensitive spectral parameters by combining principal component analysis (PCA) and correlation analysis methods; s3, taking the sensitive spectrum parameters, LAI and SPAD values as independent variables, taking the wheat grain yield as a dependent variable, and adopting partial least squares regression (PLSR) to construct a multivariable yield prediction model; and S4, performing wheat yield prediction on an independent test sample or regional scale remote sensing data by using the trained model. The method overcomes the defect that only yield sensitive spectrum parameters are used for predicting the effect, and accurate estimation of the model is achieved.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

This invention discloses a haplotype SNP molecular marker for identifying wheat grain starch content in the TaMYB44-4D gene and its application. Specifically, the SNP is T or C at position 1557bp of the sequence in SEQ ID NO.1. Based on this SNP, primers for detecting this haplotype SNP molecular marker have also been developed, and a kit containing these primers has been prepared. By PCR and polyacrylamide gel electrophoresis, the SNP and genotype can be accurately identified, thereby distinguishing between superior haplotypes (high starch content) and non-superior haplotypes (low starch content) in wheat.

The invention belongs to the technical field of heavy metal pollution treatment, and discloses a method for reducing soil cadmium activity and wheat grain cadmium content. According to the method, the low-cadmium-accumulation wheat variety is selected, the composite conditioner is applied, the low-cadmium-accumulation wheat variety is Jimai 22, and the composite conditioner is composed of mineral source potassium fulvate and zinc fertilizer. The complex passivation effect of humic acid components in mineral source potassium fulvate on soil heavy metal is utilized, the ion antagonism effect of zinc and cadmium and the low cadmium accumulation characteristic of Jimai 22 are combined, the cadmium content of wheat grains is synergistically reduced to 0.1 mg / kg or below, meanwhile, the cadmium content of the soil can be remarkably reduced, and the soil with cadmium exceeding the standard can be repaired. The treatment method provided by the invention has the advantages of simple operation, easily available raw materials, low cost, stable synergistic cadmium reduction effect and the like.

The invention relates to the technical field of heat energy engineering and efficient energy-saving equipment, and discloses a bionic heat exchanger based on seal beard turbulent flow and wheat grain communication configuration, the bionic heat exchanger comprises an inlet flow collecting cavity, an outlet flow collecting cavity and a plurality of heat exchange pipes, the heat exchange pipes are horizontally arrayed, the inlet flow collecting cavity is correspondingly arranged at one ends of the heat exchange pipes, and the outlet flow collecting cavity is correspondingly arranged at the other ends of the heat exchange pipes. The heat exchanger further comprises an inlet tube plate and an outlet tube plate, the inlet tube plate is fixedly installed on the side, facing the heat exchange tubes, of the inlet flow collecting cavity, and the outlet tube plate is fixedly installed on the side, facing the heat exchange tubes, of the outlet flow collecting cavity. The wavy torsion structures of the seal beard-imitating turbulent flow columns in the pipes induce generation of hairpin vortexes, a thermal boundary layer is efficiently destroyed, heat exchange in fluid is promoted, meanwhile, a three-dimensional networked flow channel is constructed by means of the hollow wheat grain-imitating communicating pipes between the pipes, transverse fluid mixing between pipe bundles is achieved, and the heat exchange efficiency is improved. Through the synergistic effect of the two structures, local heat accumulation and flowing dead zones are thoroughly eliminated, and the overall heat transfer performance is enhanced.

The application discloses a wheat kernel length molecular marker and application thereof, and solves the technical problem of how to provide a wheat kernel length molecular marker. The application specifically discloses the following application of any one of an InDel molecular marker or a substance for detecting the InDel molecular marker: A1) application in identification or auxiliary identification of wheat kernel length; A2) application in preparation of a product for identification or auxiliary identification of wheat kernel length; A3) application in preparation of a wheat breeding product; the InDel molecular marker is a DNA molecule with a nucleotide sequence of sequence 5. The kernel length of the deletion type wheat is longer than or longer than the kernel length of the insertion type or heterozygous type wheat, and can be used in industrial production.

The application discloses a genetic marker linked with a wheat kernel nickel ion accumulation QTL qNi.hnaas-6BS, adopts a Wheat Breeders 660K microarray chip to identify genotype data of a natural population, combines phenotype data of kernel nickel ion content of the wheat natural population, carries out whole genome association analysis (GWAS), identifies that a quantitative trait locus (QTL) qNi.hnaas-6BS for controlling wheat kernel nickel ion accumulation exists on a short arm of a 6B chromosome of the wheat, the QTL is closely linked with SNP6872, is located at the 125,482,475th nucleotide of the 6B chromosome, and the SNP6872 has C / T polymorphism. Haplotype analysis results on a population level show that when the nucleotide of the site is CC, the wheat kernel has lower nickel ion content, and is a favorable allelic genotype for limiting the accumulation of the wheat kernel nickel ion.

The application relates to the technical field of wheat kernel type genes, and provides a wheat kernel type gene TaCYT-5A TaCYT-5A The application of the molecular marker is KASP-TaCYT-5A, the marker is a SNP site (C / T) detected at 495818646 bp in a QTL cluster for controlling the wheat kernel type and kernel length traits of a 5A chromosome 484432128-495883712 bp, that is, a 4-hydroxyphenylacetaldehyde oxime monooxygenase gene TaCYT-5A The coding region is 124 bp away from the start codon. The functional KASP molecular marker of the wheat 4-hydroxyphenylacetaldehyde oxime monooxygenase gene TaCYT-5A provided by the application can be used for identifying whether an TaCYT-5A excellent allele exists in a wheat variety / strain, and applied in assisted selection breeding and breeding in combination with other known kernel type related genes.

The invention discloses a wheat alkyl resorcinol content molecular marker and application thereof. The invention provides an application of a substance for detecting the genotype of an SNP site AX-109949078 in a wheat genome in any one of the following products or preparation of any one of the following products: A1) identifying or assisting in identifying the content of alkylresorcinol in wheat grains; a2) screening or auxiliary screening of wheat with high alkyl resorcinol grains; a3) breeding wheat with high alkylresorcinol grains; a4) wheat genetic breeding, and the SNP site AX-109949078 is the 81st site of SEQ ID NO.4. Experiments prove that a wheat alkyl resorcinol content site is determined, the SNP marker closely linked with the wheat alkyl resorcinol content site is AX-109949078, a KASP primer is developed according to the corresponding marker, and molecular marker-assisted screening can be carried out on the alkyl resorcinol content.

The invention belongs to the technical field of biology, and discloses application of a KASP molecular marker related to the wet gluten content of wheat grains. One technical scheme of the invention protects the application of the composition for detecting the polymorphism or genotype of the SNP1 site in the wheat genome in any one of A1-A3: A1, the application in identification or auxiliary identification of the wet gluten content of wheat grains; a2, application in preparation of products for identification or auxiliary identification of the wet gluten content of the wheat grains; a3, application in wheat breeding or application in preparation of wheat breeding products; the SNP1 site is an SNP site in a wheat genome, the nucleotide variety of the SNP1 site is T or C, and the SNP1 site is the 251th nucleotide of SEQ ID No.1. The composition for detecting SNP1 site polymorphism and genotype can be used for preparing products for high-throughput identification of the wet gluten content of wheat grains.

The invention belongs to the technical field of agricultural remote sensing monitoring, and discloses a saline-alkali soil wheat grain total phenol content remote sensing monitoring method and system. Based on multi-source remote sensing data, meteorological and vegetation characteristics are extracted through phenological matching, and a protein content estimation model containing a salt stress nonlinear inhibition item is constructed; and establishing a quantitative relationship between the protein and the total phenol content, and finally, performing inversion by using an optimization algorithm to obtain the high-precision total phenol content, thereby realizing effective monitoring on the quality of the wheat grains in the wide-range saline-alkali soil. A'protein content-total phenol content 'secondary inversion framework is created for the first time, total phenol content monitoring is indirectly achieved through a protein remote sensing inversion technology, and the technical problem that direct spectrum inversion is low in precision is effectively solved; the saline-alkali soil specificity stress response model is creatively established, the non-linear inhibition effect of salinity on the crop physiological process is considered in the inversion process, the monitoring precision and the model adaptability are remarkably improved, and the method has the advantages of being high in monitoring precision, high in practicability, wide in application range and the like.

The application discloses a SNP marker related to wheat kernel alpha-tocopherol content and application thereof. The application provides application of a substance for detecting a SNP site Ta-6A-SNP haplotype in a wheat genome in any one of the following: A1) identifying or assisting in identifying wheat kernel alpha-tocopherol content; A2) screening or assisting in screening wheat with high alpha-tocopherol content in kernels; A3) breeding wheat with high alpha-tocopherol content in kernels; A4) wheat genetic breeding. The application finds a SNP site Ta-6A-SNP on a 6A chromosome of wheat, and develops a molecular marker according to the SNP, which is a KASP marker, has good repeatability, low cost, is beneficial to screening wheat varieties with high alpha-tocopherol content, and has important significance for cultivating new wheat varieties with high alpha-tocopherol content.

The present invention provides a use of a GAL-5B protein and a coding gene therefor in plant breeding, wherein the loss of function of the GAL-5B protein can significantly promote the accumulation of raffinose in plant seeds, while simultaneously increasing the protein content and reducing the starch content in the seeds; moreover, overexpression of GAL-5B reduces the raffinose content in the plant seeds.

The invention relates to a rice and wheat in-situ yield measurement device, system and method, and relates to the technical field of yield measurement, the rice and wheat in-situ yield measurement device comprises a mounting assembly, a separation assembly, a detection assembly and a display module, feeding, threshing, impurity removal and detection structures are integrated into a whole, the structure is compact, an operation handle is held by hand to move to any point in the field, and operation is convenient. Rice and wheat crops are inserted into the feeding opening, the separation assembly can discharge impurities such as glume shells, stems and leaves to the periphery of the roots of the plants, a covering layer is formed, water evaporation is reduced, organic matter is supplemented, additional collection bags are not needed, and zero-material-consumption and zero-waste green operation is achieved; particle parameters and position parameters are obtained and displayed through the display screen immediately, a traditional threshing machine, a traditional cleaning machine and a traditional table type metering device do not need to be carried for dispersed operation, multiple times of detection can be carried out at the same place, and the accuracy of yield indexes is improved.

The application discloses a molecular marker related to wheat kernel nickel ion accumulation, uses a natural population constructed by a team in the early stage, uses a Wheat Breeders 660K microarray chip to identify genotypic data of the natural population, combines phenotypic data of the wheat natural population kernel nickel ion content, carries out whole genome association analysis (GWAS), identifies that a quantitative trait locus (QTL) qNi.hnaas-6BL controlling the wheat kernel Ni ion accumulation exists on a long arm of a 6B chromosome of the wheat, the QTL is closely linked with SNP4008, is located at the 693,584,519th nucleotide of the 6B chromosome, and the SNP4008 has A / G polymorphism. Haplotype analysis results on the population level show that when the nucleotide of the site is AA, the wheat kernel has lower nickel ion content, and is a favorable allelic genotype; when the nucleotide of the site is GG, the wheat kernel has higher nickel ion content, and is an unfavorable allelic genotype.

The application discloses a new gene TaGW1-5B for regulating wheat kernel weight and application thereof. The application provides application of TaGW1-5B protein or TaGW1-5B gene in regulating plant traits; the plant traits are plant kernel traits and / or plant root traits; the kernel traits are kernel weight and / or kernel length; the root traits are root length and / or root surface area. The regulation is negative regulation. The application also provides application of a substance for inhibiting TaGW1-5B gene in cultivating plants with changed kernel traits and / or changed root traits; the changed kernel traits are increased kernel weight and / or increased kernel length; the changed root traits are increased root length and / or increased root surface area. The application can be used for creating new wheat germplasm with higher unit yield, and has important theoretical significance and application value for improving kernel weight.

The utility model discloses a double-color injection mold for molding a product with wheat grain texture, which comprises a fixed mold, a first fixed mold core and a second fixed mold core are arranged on the fixed mold, a wheat grain texture molding layer for molding a wheat grain texture surface on a first injection product is arranged on the bottom surface of a mold cavity of the first fixed mold core, and a second fixed mold core is arranged on the bottom surface of a mold cavity of the second fixed mold core. The second fixed mold is provided with a fixed mold cavity for molding a secondary injection product; and the movable mold is provided with two same movable mold cores, and the movable mold cores are provided with movable mold cavities used for forming a first-shot product. The wheat ear grain texture forming layer is arranged at the bottom of the mold cavity of the fixed mold core for forming the first-shot product, and wheat ear grains are formed between the first-shot product and the second-shot product, so that the wheat ear grains can be well presented on the product, and the double-shot injection mold is convenient to machine and form and high in production efficiency. And the forming quality and the presenting effect of the wheat ear grains can be well ensured.

The present application belongs to the technical field of molecular biology, and particularly relates to a wheat kernel protein content regulating gene TaGPC-6A and an encoded protein and application thereof. The wheat kernel protein content regulating gene TaGPC-6A of the present application is shown in the sequence table SEQ ID NO:1, and has a length of 1858 bp; the amino acid sequence of the encoded protein is shown in the sequence table SEQ ID NO:4, and has a length of 507. The present application proves the positive role of the gene TaGPC- 6A in regulating the wheat kernel protein content through a mutant and transgenic material. The gene TaGPC-6A of the present application can be applied to the improvement breeding of the wheat kernel protein content; the research on the gene helps to reveal the molecular genetic basis of the formation of the wheat kernel protein, and provides important gene resources for the wheat quality breeding.

The invention belongs to the technical field of wheat molecular breeding, and particularly relates to an SNP molecular marker related to wheat grain hardness on a wheat chromosome. The SNP molecule is named as qHI.1B-1; the molecular marker contains a nucleotide sequence of which the polymorphism at the 393305782bp of the wheat 1B chromosome is A or G; the wheat with the polymorphic site genotype of GG has lower grain hardness compared with the wheat with the genotype of AA. Based on the particularity, the inventor carries out further BSR analysis on an F2 population constructed based on the two varieties, and the SNP site is obtained. Based on the site, a certain foundation can be laid for early and rapid detection and analysis of the wheat hardness character, the subsequent selection efficiency can be improved, the breeding period can be shortened, and wheat varieties with ideal grain hardness can be rapidly identified and cultivated.

This invention discloses an InDel molecular marker associated with wheat grain length and its application. This InDel molecular marker can be used to identify wheat grain length traits or for wheat breeding. The primer sequences for amplifying the InDel molecular marker include an upstream primer F and a downstream primer R. The sequence of the upstream primer F is shown in SEQ ID NO.1, and the sequence of the downstream primer R is shown in SEQ ID NO.2. The molecular marker provided by this invention has been validated in multiple varieties, and the detection results are highly consistent with the grain length phenotype. It can be used to extract leaf DNA at the seedling stage for early selection, providing a direct and effective molecular tool for the genetic improvement of wheat grain length. It can be widely applied in high-yield and high-quality wheat breeding practices.

This invention discloses a molecular marker for the QTL QFAC-3BS of ferulic acid content in wheat grains and its application. This invention belongs to the field of molecular breeding, specifically relating to the molecular marker for the QTL QFAC-3BS of ferulic acid content in wheat grains and its application. The method for identifying or assisting in the identification of ferulic acid content in wheat grains according to this invention includes detecting the genotype of a SNP site in the genome of the wheat to be tested, and identifying or assisting in the identification of ferulic acid content in wheat grains based on the genotype. The SNP site is a site on wheat chromosome 3B, and its nucleotide type is A or G, located at nucleotide position 81 of sequence 1 in the sequence listing. The KASP marker and its specific primer set developed in this invention can be used to detect the genotype of the SNP site, achieving molecular marker-assisted screening for ferulic acid content in grains, which is of great significance for breeding wheat varieties with increased ferulic acid content in grains.

The invention discloses a cultivation method for improving the germination rate of wheat grains harvested in advance under the breeding acceleration condition, and belongs to the technical field of agricultural planting technology.The cultivation method comprises the following steps that wheat seeds harvested in advance are pretreated and then subjected to illumination germination treatment; the wheat seeds harvested in advance refer to wheat seeds harvested on the 15th-30th day after wheat blooming; the pretreatment specifically comprises one or more of drying treatment, hydrogen peroxide treatment, variable-temperature treatment and gibberellin treatment; the method aims at solving the problems that under the planting acceleration condition, the germination rate of the unripe wheat seeds is low, and the growth consistency is poor. By means of the method, the germination rate of wheat seeds harvested in advance is remarkably increased to 77.78% from 43.33%, the growth consistency of offspring is enhanced, the growth cycle is shortened, a reliable and efficient solution is provided for rapid planting and efficient breeding of wheat, and the method has important agricultural application value and practical popularization significance.

This invention discloses a method for wheat grain quality detection and functional gene analysis based on hyperspectral imaging technology, belonging to the field of agricultural testing technology. The method includes: extracting hyperspectral indices; measuring wheat grain quality indicators as manual measurements; assigning weight coefficients to each quality indicator to digitize the wheat quality phenotype; extracting characteristic wavelengths to establish predictive models for each indicator and wheat quality values; performing GWAS on hyperspectral indices and chemical indicators as phenotypic traits with genotypes to screen SNP loci, conduct co-location analysis, obtain candidate genes, and perform functional verification. This invention, through hyperspectral technology combined with chemometric modeling, is suitable for real-time monitoring during wheat quality breeding and processing; by combining spectral phenotype with GWAS gene mining, it overcomes the limitations of traditional quality detection that only serves phenotypic screening, forming a closed loop of "non-destructive detection - gene localization," providing targeted genes for wheat quality improvement.

The utility model provides a press machine for oat food raw materials, which belongs to the field of oat processing, and comprises a processing box body, two pressure rollers are rotatably arranged in the processing box body; a feeding mechanism; a guide rod fixedly connected with the material guide plate is slidably connected into the arc-shaped groove, an eccentric roller which abuts against the pressure roller and rotates synchronously with the pressure roller is arranged at the bottom of the material guide plate, and the eccentric roller rotates to drive the material guide plate to swing up and down. The eccentric roller rotates to enable the part, close to the hopper, of the material guide plate to swing up and down and drive the anti-blocking rod to swing up and down at the same time, the bottom of the hopper is not prone to being blocked through swing of the anti-blocking rod, and therefore wheat grains can be stably conveyed to the material guide plate and can be slowly conveyed into the processing box body through vertical swing of the material guide plate. And therefore, excessive wheat grains are prevented from entering the space between the pressure rollers to influence flattening of the oats, and the quality of the oats can be ensured.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a wheat grain protein content related protein, a coding gene thereof and application.The wheat grain protein content related protein is a TaNPF5.5-2A protein and / or a TaNPF5.5-2B protein.The research shows that the grain protein content and the total nitrogen content of the TaNPF5.5-2B overexpression transgenic wheat strain have a significant advantage.The present application screens and mines a new wheat grain protein content related gene, which not only provides a theoretical clue for the molecular genetic research of the wheat grain protein content, but also provides important gene resources for the molecular breeding of the wheat quality.

The invention belongs to the technical field of wheat germplasm identification, particularly relates to a wheat grain zinc and iron content marking method and application, discloses four SNP (Single Nucleotide Polymorphism) sites QGZn-4B, QGZn-7B, QGFe-2A and QGFe-3B which are stably associated with the zinc content and the iron content of wheat grains, and solves the core pain points of poor molecular marker stability and indefinite association in the prior art. And a reliable genetic target is provided for accurate identification of the zinc and iron content of wheat. According to the invention, a KASP specific primer combination developed for four SNP loci is also adopted, so that efficient genetic typing of a wheat material is realized, a complicated sequencing process is not needed, the operation is simple and convenient, the specificity is high, and the problems of long detection period, complicated operation and high cost of a traditional molecular marker are effectively avoided. The blank of efficient tools in current wheat zinc and iron content molecular marker assisted breeding is effectively filled, and the method has important significance in promoting innovation and upgrading of a wheat nutritional quality breeding technology.

The application discloses application of wheat B1 protein and related biological materials thereof in regulating wheat kernel size, kernel weight and protein content. The application causes B1 protein not to be expressed or to be down-regulated by knocking out a coding gene of B1 protein which inhibits awn elongation in a wheat genome, and experimental results show that the absence of B1 protein expression in the wheat genome can improve the kernel size, kernel weight and protein content of awnless wheat. The application can be applied to high-yield and high-quality breeding and variety improvement of wheat.

This invention discloses a molecular marker for detecting pleiotropic loci of zinc, iron, and thousand-grain weight in wheat grains and its application, belonging to the field of biotechnology. The technical problem this invention aims to solve is: how to identify or assist in the identification of thousand-grain weight / zinc / iron content traits in wheat grains. To solve this problem, this invention provides the AX-109500250 locus and a substance for detecting the genotype of the AX-109500250 locus in the identification or assistance in the identification of thousand-grain weight / zinc / iron content traits in wheat grains. The AX-109500250 locus is a SNP site in the wheat genome, such as nucleotide 48 of SEQ ID No. 1, and its nucleotide type is C or T. The molecular marker provided by this invention has important application value for molecular breeding of high-zinc / high-iron / high-grain-weight wheat.

The invention relates to a method for increasing the content of gamma-aminobutyric acid (GABA) in wheat malt seedlings through pulsed electric field synergistic stress germination treatment, which belongs to the technical field of agricultural product processing and is characterized in that wheat grains are used as raw materials, wheat malt seedlings are cultivated through soaking disinfection, pulsed electric field treatment and stress germination, and wheat malt seedling powder rich in GABA is obtained through drying and crushing. The treatment time of the pulsed electric field is short, the improvement effect is remarkable, the content of GABA in the wheat malt seedlings can be effectively improved, and the content of GABA in the wheat malt seedlings reaches 360-450 mg / kg DW.