Establishment method for brown mushroom three-level strain propagation system

A technology of tertiary strains and establishment methods, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of long breeding time, and achieve the effects of fast growth speed, wide fruiting temperature range, and shortened growth cycle.

Active Publication Date: 2013-06-19
天津市金三农农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the brown mushroom has a good market prospect, but there are few researches on its cultivation method at present, especially compared with the Agaricus bisporus tertiary bacterial strain breeding time is longer, the present invention aims at preparing the brown

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Establishment of three-stage propagation system of brown mushroom

[0042] 1.1 Preparation of mother species:

[0043] (1) The formula of the mother seed medium, that is, peat soil medium (g / L): 600g peat soil, 20g glucose, 100g wheat grains, 1.5g magnesium sulfate, 1g potassium dihydrogen phosphate, 2g peptone, and 1000mL water.

[0044] (2) Preparation of medium. Take an appropriate amount of peat soil and wheat grains, add a certain amount of clear water and boil for 20-30 minutes, filter with 4 layers of gauze to obtain the filtrate. Add magnesium sulfate, potassium dihydrogen phosphate, peptone, glucose, and agar to the filtrate. After the medicine is dissolved, make up water and pack into test tubes.

[0045] (3) Sterilization. Sterilize at 121°C for 20 minutes. After sterilization, remove the pendulum bevel.

[0046] (4) Inoculation and cultivation. Strictly inoculate the brown mushroom hyphae in the middle of the slope according to the aseptic technique. ...

Embodiment 2

[0066] A method for establishing a brown mushroom three-stage strain propagation system

[0067] (1) Preparation of mother species:

[0068] 1) The formula of mother seed medium, that is, peat soil medium (g / L): 600g peat soil, 20g glucose, 100g wheat grains, 1.5g magnesium sulfate, 1g potassium dihydrogen phosphate, 2g peptone, 1000mL water;

[0069] 2) Preparation of culture medium: Take appropriate amount of peat soil and wheat grains, add 1000ml of water and boil for 30 minutes, filtrate; add magnesium sulfate, potassium dihydrogen phosphate, peptone, glucose, agar to the filtrate, after the medicine is dissolved, add water, Pack test tubes;

[0070] 3) Sterilization: 121°C, sterilize for 20 minutes, after the sterilization, take out the pendulum;

[0071] 4) Inoculation and cultivation: Inoculate the mycelia of brown mushrooms in the middle of the slope in strict accordance with the aseptic technique; cultivate at a constant temperature of 25°C until the tube is full; ...

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Abstract

The invention discloses an establishment method for a brown mushroom three-level strain propagation system. The establishment method for the brown mushroom three-level strain propagation system comprises three-level strain propagation of stock cultures, liquid strains and cultispecies, wherein the formula of culture mediums of the stock cultures comprises, by weight (g/L), 600g of turfy soil, 20g of glucose, 100g of wheat grains, 1.5g of magnesium sulfate, 1g of monopotassium phosphate, 2g of peptone and 1000mL of water, the formula of the liquid strains comprises, by weight (g/L), 200g of potatoes, 20g of glucose, 2g of peptone, 2g of monopotassium phosphate, 1g of magnesium sulfate and 10.01g of VB, the pH (potential of hydrogen) is natural, and the cultispecies comprise, by weight (%), 92% of wheat grains, 5% of gypsum and 3% of turfy soil. Compared with a traditional solid state three-level strain, through using the propagation system, the propagation cycle is shortened by 38 days. The establishment method for the brown mushroom three-level strain propagation system is an optimum scheme, and has a certain guiding significance to the mass industrialized production of brown mushrooms.

Description

technical field [0001] The invention provides a cultivation method related to brown mushrooms, more specifically a method for multiplying brown mushroom tertiary strains. Background technique [0002] brown mushrooms ( Agaricus brunnescens Peck), is a rare species of mushrooms, belonging to Basidiomycotina (Basidiomycotina), Agaritales (Agaritales), mushroom family (Agaricaceae), mushroom genus ( Garicus ). Brown mushrooms, commonly known as brown mushrooms, are Bohemian subspecies. The strain has high yield, excellent mushroom quality, and fresh and tender taste. The mushroom cap is natural brown, the stipe is white, and the meat is dense and firm. All current white Agaricus species are white varieties of the brown mushroom, which means that the brown mushroom is the wild ancestor of the white Agaricus bisporus. Brown mushrooms, also known as white mushrooms and champignons, belong to Basidiomycetes, Agaricaceae, Agaricaceae, and Mushrooms. Brown mushrooms are rich in...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 班立桐黄亮王玉杨丽维孙茜孙少起
Owner 天津市金三农农业科技开发有限公司
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