Establishment method for brown mushroom three-level strain propagation system
A technology of tertiary strains and establishment methods, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of long breeding time, and achieve the effects of fast growth speed, wide fruiting temperature range, and shortened growth cycle.
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Embodiment 1
[0041] Establishment of three-stage propagation system of brown mushroom
[0042] 1.1 Preparation of mother species:
[0043] (1) The formula of the mother seed medium, that is, peat soil medium (g / L): 600g peat soil, 20g glucose, 100g wheat grains, 1.5g magnesium sulfate, 1g potassium dihydrogen phosphate, 2g peptone, and 1000mL water.
[0044] (2) Preparation of medium. Take an appropriate amount of peat soil and wheat grains, add a certain amount of clear water and boil for 20-30 minutes, filter with 4 layers of gauze to obtain the filtrate. Add magnesium sulfate, potassium dihydrogen phosphate, peptone, glucose, and agar to the filtrate. After the medicine is dissolved, make up water and pack into test tubes.
[0045] (3) Sterilization. Sterilize at 121°C for 20 minutes. After sterilization, remove the pendulum bevel.
[0046] (4) Inoculation and cultivation. Strictly inoculate the brown mushroom hyphae in the middle of the slope according to the aseptic technique. ...
Embodiment 2
[0066] A method for establishing a brown mushroom three-stage strain propagation system
[0067] (1) Preparation of mother species:
[0068] 1) The formula of mother seed medium, that is, peat soil medium (g / L): 600g peat soil, 20g glucose, 100g wheat grains, 1.5g magnesium sulfate, 1g potassium dihydrogen phosphate, 2g peptone, 1000mL water;
[0069] 2) Preparation of culture medium: Take appropriate amount of peat soil and wheat grains, add 1000ml of water and boil for 30 minutes, filtrate; add magnesium sulfate, potassium dihydrogen phosphate, peptone, glucose, agar to the filtrate, after the medicine is dissolved, add water, Pack test tubes;
[0070] 3) Sterilization: 121°C, sterilize for 20 minutes, after the sterilization, take out the pendulum;
[0071] 4) Inoculation and cultivation: Inoculate the mycelia of brown mushrooms in the middle of the slope in strict accordance with the aseptic technique; cultivate at a constant temperature of 25°C until the tube is full; ...
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