24 results about "Glucose & Protein" patented technology

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The present application relates to a kind of skin care oil compositions for sensitive skin damage repair and its application, the skin care oil compositions include microbial oil and base oil, the microbial oil is with perilla seed powder, almond powder, camellia seed powder, glucose, protein peptone, yeast extract as substrate, using schizochytrium and lactobacillus plantarum to ferment substrate, the microbial oil obtained by fermentation has good effect of repairing skin damage.

The invention relates to the technical field of microbial strains, and provides torulaspora delbrueckii, and the preservation number of the torulaspora delbrueckii is CGMCC (China General Microbiological Culture Collection Center) NO.7.581. The invention further discloses a screening and identifying culture medium which is used for screening, identifying and culturing the torulaspora delbrueckii. The screening and identifying culture medium comprises a nutrient agar culture medium, a sugar fermentation culture medium, an assimilation carbon source culture medium, an assimilation nitrogen source culture medium and a YPD basal culture medium. The torulaspora delbrueckii is screened from the mousse fruit wine and is used for mousse fermentation, the defect that the taste and flavor of existing saccharomycetes are similar in the fermentation process can be overcome, and the traditional flavor and taste of mousse can be reserved. And the contents of glucose, peptone and yeast extract powder in the culture medium are optimized through a single factor test and a response surface method, so that the amount of torulaspora delbrueckii is further increased, and theoretical guidance is provided for industrial application of mousalaisi brewing.

The invention relates to the technical field of microbial fermentation, and discloses a moisturizing and soothing bifidobacterium lactis SF-B-20 fermentation product as well as a preparation method and application thereof, and the preservation number of bifidobacterium lactis SF-B-20 is CGMCC (China General Microbiological Culture Collection Center) No.20924. The moisturizing and soothing bifidobacterium lactis SF-B-20 fermentation product is prepared by fermenting a fermented substrate with bifidobacterium lactis SF-B-20, and the fermented substrate comprises a Chinese herbal medicine extract and a fed-batch culture medium; chinese herbal medicine raw materials for preparing the Chinese herbal medicine extract comprise the following components: silver birch juice, radix ampelopsis, astragalus membranaceus, aloe, purslane, liquorice, chamomile and radix sophorae flavescentis, the fed-batch culture medium comprises the following components: glucose, peptone, yeast extract powder and tremella polysaccharide, and the moisturizing and soothing bifidobacterium lactis SF-B-20 yeast has moisturizing and soothing effects and is safe and non-irritating.

The present invention provides a green and safe method for synthesizing mannitol, belonging to the technical field of mannitol biosynthesis; the synthesis method includes steps of primary seed culture, secondary seed culture and fermentation culture; in the secondary seed culture, the primary seed culture solution is inoculated into a secondary seed culture medium to obtain a secondary seed culture solution; the components of the secondary seed culture medium are glucose, peptone, yeast extract, dipotassium hydrogen phosphate, glycerol, inositol, trehalose, and an additive; the present invention adopts two-stage seed culture and fermentation culture, effectively promotes the synthesis of yeast in the direction of mannitol, shortens the fermentation time, improves the yield and quality of mannitol, greatly improves the production efficiency, and has good biological safety. The culture conditions are mild, avoiding dangerous and energy-consuming operations such as high temperature and high pressure, with high safety, and conforming to the concept of green and safe production.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The present invention belongs to the technical field of microbial fertilizers, and in particular, relates to an agricultural microbial agent and a preparation method and application thereof, wherein the agricultural microbial agent comprises the following raw materials in mass percentage: 4-8% brown sugar, 1-3% glucose, 12-18% peptone, 8-12% beef extract, 0.5-1.5% edible salt, 8-12% yeast extract, 1-3% Bacillus megaterium, 1-3% peptone-like bacillus, 1-3% Bacillus subtilis, and the balance is water. The number of effective live bacteria in the agricultural microbial agent of the present invention is ≥200 million / mL, and the rate of foreign bacteria is ≤10%, which meets the use standard of the agricultural microbial agent. The application of the microbial agent of the present invention can not only significantly increase the yield of peppers and increase economic benefits, but also significantly increase the length, width and flesh thickness of peppers, reduce the nitrate content in peppers, and make its content far lower than the maximum limit standard of nitrate in pollution-free peppers, effectively improve the quality of peppers, and effectively reduce the amount of chemical fertilizers, and has various functions.

The application provides a biological skin activity fermentation liquor with multiple effects, a preparation method thereof and a cosmetic, and relates to the cosmetic field.The method comprises the following steps: mixing camellia oleifera seed meal, protease, cellulase, lipase and water to obtain a first mixture; performing enzymolysis on the first mixture to obtain a fermentation substrate; performing second mixing on the fermentation substrate, glucose, peptone, yeast powder, disodium hydrogen phosphate and citric acid, and sterilizing to obtain a fermentation culture medium; activating bacillus velezensis through an LB culture medium to obtain a seed liquid; performing third mixing on the seed liquid and the fermentation culture medium, and performing fermentation to obtain a camellia oleifera seed meal fermentation liquor; performing centrifugation on the camellia oleifera seed meal fermentation liquor to obtain a centrifuged fermentation liquor; performing fourth mixing on the centrifuged fermentation liquor and polysaccharide-degrading enzyme, performing enzymolysis, and performing filtration to obtain the biological skin activity fermentation liquor with multiple effects.The method not only improves the high-value utilization of the camellia oleifera seed meal, but also obtains the biological skin activity fermentation liquor with high safety and outstanding activity.

The invention relates to the technical field of microbial culture, in particular to an efficient tremella aurantialba liquid strain culture medium and a preparation and culture method. The feed comprises the following components in percentage by mass: 0.5% of corn flour; 3% of glucose; 0.4% of peptone; 0.2% of monopotassium phosphate; 0.1% of magnesium sulfate; 0.001% of vitamin B; and the balance of water. The method has the beneficial effects that 1, the growth efficiency is obviously improved; 2, the strain quality is stable and controllable; 3, the advantage of production cost is outstanding; operation is easy and convenient, and popularization is easy.

The invention relates to the technical field of biological medicines, and particularly discloses a culture medium for high-density expression of an FMDV 3ABC antigen by escherichia coli and a fermentation culture method. The invention discloses a culture medium for high-density expression of an FMDV 3ABC antigen by escherichia coli. The culture medium comprises a basic culture medium and a fed-batch culture medium, the basic culture medium is prepared from tryptone, a yeast extract, glycerol, a stabilizer, ampicillin sodium, NaCl, K2HPO4, MgSO4 and CaCO3; the solvent is water; the fed-batch culture medium is prepared from glucose, peptone, yeast extract, glycerol, ammonium sulfate, a stabilizer and ampicillin sodium; the solvent is water; the stabilizer is prepared from polydimethylsiloxane aqueous emulsion and palmarosa oil. By utilizing the technical scheme provided by the invention, the fermentation culture time in the fermentation culture method for expressing the FMDV 3ABC antigen by escherichia coli can be shortened, and the obtained antigen product is high in yield and high in purity.

The present invention belongs to the technical field of esophageal squamous cell carcinoma inhibitors, and particularly relates to an EI24 agonist and its application in the preparation of a preparation for inhibiting the proliferation of esophageal squamous cell carcinoma. The EI24 agonist is pentagalloyl glucose, FOXP1 protein or a combination of both. It can targetedly increase the expression level of EI24 and inhibit the proliferation of esophageal squamous cell carcinoma cells, and has good application prospects in inhibiting esophageal squamous cell carcinoma.

The present invention relates to herbicidal mixtures and their methods and uses for controlling undesirable vegetation in conventional and tolerant, e.g. glufosinate-tolerant, cotton, wherein the herbicidal mixtures comprise L-glufosinate and and at least one herbicidal compound II selected from clethodim, fluazifop, fluazifop-butyl, fluazifop-P, fluazifop-P-butyl, haloxyfop, haloxyfop-methyl, haloxyfop-P, haloxyfop-P-methyl, metamifop, quizalofop, quizalofop-ethyl, quizalofop-tefuryl, quizalofop-P, quizalofop-P-ethyl, quizalofop-P-tefuryl, chlorimuron, chlorimuron-ethyl, thifensulfuron, thifensulfuron-methyl, trifloxysulfuron, tritosulfuron, imazamethabenz, imazamethabenz-methyl, imazamox, imazamox ammonium, imazapic, imazapic ammonium, imazapyr, imazapyr isopropylammonium, imazaquin, imazethapyr, imazethapyr ammonium, pyrithiobac, pyrithiobac-sodium, amicarbazone, atrazine, prometryn, diuron, fluometuron, thiadiazuron, carfentrazone, carfentrazone-ethyl, flumiclorac, flumiclorac-pentyl, flumioxazin, fluthiacet, fluthiacet-methyl, fomesafen, pyraflufen, pyraflufen-ethyl, oxyfluorfen, saflufenacil, sulfentrazone, tiafenacil, trifludimoxazin, norflurazon, picolinafen, clomazone, topramezone, fenquinotrione, mesotrione, bicyclopyrone, isoxaflutole, tembotrione, tolpyralate, glyphosate, glyphosate dimethylammonium, glyphosate-isopropylammonium, glyphosate-potassium, glyphosate-trimesium (sulfosate), pendimethalin, trifluralin, acetochlor, alachlor, butachlor, dimethenamid, dimethenamid-P, metazachlor, metolachlor, S-metolachlor, pyroxasulfone, 2,4-D and its salts and esters, dicamba and its salts and esters, fluroxypyr, fluroxypyr-butometyl, fluroxypyr-meptyl, quinclorac, quinclorac dimethylammonium, quinmerac, cinmethylin, endothal, cycloxydim, sethoxydim, diflufenzopyr, diflufenzopyr-sodium, bentazone and bentazone-sodium and other compounds.

The present application provides a grifola frondosa liquid culture medium and its culture method and application, relates to the fermentation technical field.The present application provides a grifola frondosa liquid culture medium, including glucose, proteose peptone, KH2PO4, MgSO4, vitamin B1, gibberellin and tyrosol, the culture medium can significantly improve grifola frondosa growth and the secretion of extracellular polysaccharide, and can be used for the preparation of food, medicine and health care product.

The present invention provides a fermentation system and a fermentation method for vancomycin, belonging to the technical field of microbial fermentation. The vancomycin fermentation system of the present invention comprises a basal medium and a feeding medium; the basal medium is glucose 10 - 30 g / L, peptone 5 - 15 / L, yeast extract 2 - 8 g / L, potassium dihydrogen phosphate 1 - 3 g / L, magnesium sulfate 0.5 - 1.5 g / L; the feeding medium is a glucose peptone mixture, glucose 500 g / L, peptone 250 - 1000 g / L; during the fermentation process, the feeding medium is added regularly. The present invention can effectively improve the fermentation ability of Amycolatopsis orientalis, increase the yield of vancomycin, and reduce the content of impurities - demethylvancomycin in the fermentation broth.

The invention provides a lactobacillus plantarum fermentation culture medium and a preparation method thereof, the culture medium is composed of glucose, peptone, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium phosphate, the content of the components of the culture medium is as follows: 10-80 g / L of glucose, 20-80 g / L of peptone, 0.05-1 g / L of magnesium sulfate heptahydrate, 0.05-1 g / L of manganese sulfate monohydrate and 0.5-4 g / L of dipotassium phosphate, and the pH value of the culture medium is 6-7.5. The preparation method of the culture medium comprises the following steps: S1, accurately preparing the components of the culture medium; s2, independently dissolving glucose, and independently dissolving other components; s3, respectively carrying out high-pressure steam sterilization treatment on the two parts of solutions; s4, cooling the two parts of sterilized solution, mixing under sterile operation, and uniformly stirring to adjust the pH value of the solution; and S5, cooling the culture medium solution in a sterile environment, and storing in a low-temperature environment of 4 DEG C. The lactobacillus plantarum fermentation culture medium prepared by the invention is simple in component, stable in performance, low in price and high in applicability, the viable bacterium rate is 3 times that of an MRS culture medium, and the lactobacillus plantarum fermentation culture medium has a relatively great market application prospect.

The invention discloses a composition rich in natural B vitamins as well as a preparation method and application thereof, and belongs to the field of sports nourishment raw materials. The composition is prepared by fermenting raw materials including glucose, peptone, yeast extract, magnesium sulfate and the like by adopting mixed strains including saccharomyces cerevisiae ATCC26603, candida utilis ATCC9950 and rhodotorula glutinis ATCC32765; and treating by combining an ultrahigh pressure synergistic enzyme method process to obtain yeast extract powder which is rich in natural B vitamins and is relatively high in yeast protein yield. The yeast extract prepared by the invention has excellent muscle injury protection and repair effects, and the sports nourishment prepared by compounding the yeast extract with an embedding medium and an antioxidant can reduce muscle injury after sports, promote muscle development and meet core nutritional requirements of sports crowds.

The invention discloses a fermentation culture method of clostridium butyricum, and belongs to the technical field of microbial fermentation. The invention provides a clostridium butyricum fermentation medium. The clostridium butyricum fermentation medium comprises glucose, peptone, corn flour, dipotassium phosphate, monopotassium phosphate, licochalcone D, ganoderic acid A and pectin. The invention also provides a method for fermenting and culturing clostridium butyricum based on the fermentation culture medium. The method comprises the following steps: culturing pseudomonas putida, collecting fermentation liquor, separating supernatant, regulating pH by using malic acid, uniformly mixing with the fermentation culture medium, and inoculating clostridium butyricum seed liquid for anaerobic culture. Experimental results show that the clostridium butyricum fermentation culture method provided by the invention can remarkably improve the multiplication capacity of the clostridium butyricum, and after fermentation culture is performed for 48 hours, the content of viable bacteria in fermentation liquor is as high as 524.7 * 10 < 8 > CFU / mL. The invention provides a new strategy for efficient culture of clostridium butyricum and preparation of micro-ecological products, and has a wide application prospect.

The invention relates to the technical field of strain culture, and particularly discloses a nutrient solution, an expanding propagation solution and a culture method for culturing colored bean puffball strains. The nutrient solution provided by the invention comprises 18-21g / L of glucose, peptone, yeast powder, monopotassium phosphate, citric acid, calcium chloride, diammonium hydrogen phosphate, vitamin B1, wort, magnesium sulfate heptahydrate, ferric chloride, boric acid, manganese chloride tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate and sodium molybdate dehydrate. The nutrient solution, the solid culture medium and the propagation liquid provided by the invention are rich and balanced in nutrition, and are suitable for culture and propagation of colored bean puffball, so that the production cycle of a stock culture stage and a production seed propagation stage are obviously shortened, and the experiment cost is greatly reduced; and the components are clear and stable, the components are accurately proportioned, the quality is stable, the difference between batches is small, and the stability and repetition of experimental results are facilitated.

The present invention provides a culture medium and use thereof in water toxicity colorimetric detection. The culture medium comprises glucose, peptone, NaCl, a beef extract, and a ferric salt. The solubility of the ferric salt in water is not high, such that part of the ferric salt remains resuspended in a solution along with cultured bacteria to continuously release Fe3+ after centrifugation and washing, thereby successfully constructing a water toxicity colorimetric detection sensor which is simple, environment-friendly, and visible to naked eyes.

The invention belongs to the technical field of coal mine fire prevention and extinguishing, and particularly relates to microbial gel foam for preventing coal spontaneous combustion. The microbial gel foam is prepared from the following components in percentage by mass: 0.3 to 0.5 percent of composite foaming agent, 0.03 to 0.12 percent of foam stabilizer, 0.2 to 0.6 percent of gelling agent, 0.1 to 0.3 percent of cross-linking agent, 5 to 25 percent of nutrient substance, 5 to 20 percent of activated oxygen-consuming microbial solution and the balance of water. The composite foaming agent is formed by mixing AES and APG, the foam stabilizer is XG, the gelling agent is SA, and the cross-linking agent is CL; the nutrient substance consists of glucose, peptone and yeast extract powder, and the activated oxygen-consuming microorganism solution contains enterobacter and saccharomycetes. According to the microbial gel foam, the concentration of environmental oxygen can be reduced to 5%, the concentration of produced carbon dioxide is as high as 60%, and coal oxygen reaction can be effectively hindered after the gel foam collapses.

The invention provides a culture medium for promoting growth of genetically modified bacillus subtilis and a preparation method thereof. The culture medium for promoting growth of genetically modified bacillus subtilis is prepared from the following raw materials: pure fermentation liquor, anhydrous dextrose, peptone, yeast extract, monopotassium phosphate, calcium carbonate and distilled water. The culture medium for promoting the growth of the genetically modified bacillus subtilis can relieve the metabolic pressure caused by the gene modification of the bacillus subtilis, improve the growth rate and metabolic activity of cells, ensure the high-throughput expression of riboflavin, realize the efficient and stable culture of strains, lay a foundation for industrial production, and have wide application prospects. The problem of growth inhibition of the bacillus subtilis after gene modification is solved.

The invention relates to a skin care oil composition aiming at sensitive skin injury repair and application thereof, the skin care oil composition comprises microbial oil and basic oil, the microbial oil takes perilla seed powder, almond powder, camellia seed powder, glucose, peptone and yeast extract as substrates, the substrates are fermented by utilizing schizochytrium limacinum and lactobacillus plantarum, and the basic oil is obtained by fermenting the substrates. The microbial oil obtained by fermentation has a good effect of repairing skin injury.

The application provides a Lactobacillus plantarum fermentation medium and a preparation method thereof. The components of the medium are composed of glucose, peptone, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium hydrogen phosphate, and the content of the medium components is as follows: 10-80 g / L of glucose, 20-80 g / L of peptone, 0.05-1 g / L of magnesium sulfate heptahydrate, 0.05-1 g / L of manganese sulfate monohydrate and 0.5-4 g / L of dipotassium hydrogen phosphate, and the pH of the medium is 6-7.5. The preparation method of the medium comprises the following steps: S1, accurately preparing each component of the medium; S2, separately dissolving the glucose and the remaining components; S3, respectively sterilizing the two solutions by high-pressure steam; S4, cooling the two sterilized solutions, mixing the two solutions under aseptic operation, uniformly stirring the solution and adjusting the pH value of the solution; and S5, cooling the medium solution in a sterile environment and storing the medium solution in a low-temperature environment at 4 DEG C. The Lactobacillus plantarum fermentation medium prepared by the application has the advantages of simple components, stable performance, low price, strong applicability, high viable cell count (3 times of that of MRS medium) and great market application prospect.

This invention provides a liquid culture medium for the preparation of *Auricularia auricula-judae* spawn in high-altitude areas, comprising a primary fermentation liquid culture medium, a secondary fermentation liquid culture medium, and a fermenter liquid culture medium. The primary fermentation liquid culture medium has a pH of 6-7 and comprises: 5-10g soluble starch, 0-5g soybean meal, 10-15g glucose, 1-3g peptone, 2g potassium dihydrogen phosphate, 0.5g magnesium sulfate, and 10mg vitamin B1 per liter of medium. The secondary fermentation liquid culture medium also has a pH of 6-7 and comprises: 5-10g soluble starch, 0-5g soybean meal, 10-15g glucose, 1-3g peptone, 2g potassium dihydrogen phosphate, and 0.5g magnesium sulfate per liter of medium. The fermenter liquid culture medium is prepared by adding 1.5%-2.0% by weight of sawdust powder to the primary fermentation liquid culture medium. This invention also provides a method for preparing *Auricularia auricula-judae* spawn in high-altitude areas. The method of this invention can produce the original strain of golden ear fungus in about 20 days, with a short production cycle, and is suitable for the rapid production of original strain of golden ear fungus in high-altitude areas.