81 results about "Glucose & Protein" patented technology

The invention discloses an establishment method for a brown mushroom three-level strain propagation system. The establishment method for the brown mushroom three-level strain propagation system comprises three-level strain propagation of stock cultures, liquid strains and cultispecies, wherein the formula of culture mediums of the stock cultures comprises, by weight (g/L), 600g of turfy soil, 20g of glucose, 100g of wheat grains, 1.5g of magnesium sulfate, 1g of monopotassium phosphate, 2g of peptone and 1000mL of water, the formula of the liquid strains comprises, by weight (g/L), 200g of potatoes, 20g of glucose, 2g of peptone, 2g of monopotassium phosphate, 1g of magnesium sulfate and 10.01g of VB, the pH (potential of hydrogen) is natural, and the cultispecies comprise, by weight (%), 92% of wheat grains, 5% of gypsum and 3% of turfy soil. Compared with a traditional solid state three-level strain, through using the propagation system, the propagation cycle is shortened by 38 days. The establishment method for the brown mushroom three-level strain propagation system is an optimum scheme, and has a certain guiding significance to the mass industrialized production of brown mushrooms.

The invention discloses a bio-control composite bacterial agent which comprises, by weight, bacterial strains including 8-12 parts of purpureocillium lilacinum, 18-22 parts of pseudomonas fluorescens, 18-22 parts of bacillus cereus, 8-12 parts of bacillus mucilaginosus, 18-22 parts of bacillus subtilis, 8-12 parts of bacillus megaterium, 4-6 parts of bacillus thuringiensis and 4-6 parts of trichoderma viride and a culture medium comprising 0.8-1.2 parts of beef extract, 2.5-4 parts of potassium dihydrogen phosphate, 1.3-1.8 parts of urea, 10-12 parts of molasses, 1.5-2.5 parts of amino acid, 2.5-3.5 parts of glucose, 0.3-0.6 part of peptone, 0.25-0.4 part of sodium chloride and 0.35-0.6 part of potassium nitrate. The invention further comprises a preparation method of the bacterial agent. The bio-control composite bacterial agent has good effect on solving current problems of soil improvement, environment protection of agriculture and waste straw rubbish and can improve soil and accelerate crop growth and organic matter decomposition.

The invention discloses halomonas TF-1 and application thereof. The strain of the halomonas TF-1 is obtained from an oil-water well sample of the Shengli Oil Field under the conditions of 37 DEG C temperature and ordinary pressure by using a high-salt culture medium containing 6.0% of NaCl through enrichment culture screening, temperature-resistant salt-resistant performance investigation, repeated screening and passage, is named as halomonas sp.TF-1, and the preservation number of the halomonas TF-1 is CGMCC No.10619. The nutrient culture medium of the halomonas TF-1 is prepared from 0.3 mg/L of glucose, 0.3 mg/L of peptone, 0.3 mg/L of yeast powder, 0.27 mg/L of K2HPO4 and 6 mg/L sodium chloride, and a pH value is 7.0 -7.5. The halomonas TF-1 grows with crude oil as a carbon source, the bacterial concentration is greater than 5*108 per milliliter. The emulsification viscosity reduction rate of fermentation liquid is higher than 90%. The recovery ratio improved in a physical simulation experiment is higher than 15%. The average oil increase amount of microbial single well stimulation is more than 250 tons. An averagely prolonged hot washing period of microbial paraffin control is greater than 120d. Therefore, the halomonas TF-1 can be widely applied to a field test of microbial single well treatment.

The invention relates to a rice processing technique for manufacturing of cereals, and in particular relates to a balsam pear coarse cereal processing technique. The balsam pear coarse cereal processing technique comprises the following key steps of: (1) uniform mixing of materials; (2) restrictive gelatinization; (3) cutting molding; (4) initial cooling; (5) microwave drying; and (6) after-cooling. The balsam pear coarse cereal prepared by the balsam pear coarse cereal processing technique adopting the technical scheme of the invention can reduce blood sugar, promote human body to metabolize glucose, protein and fat, and is balanced in nutriments, convenient to cook and good in taste.

The invention relates to glucose albumen powder. The glucose albumen powder consists of the following raw materials in part by weight: 15 to 30 parts of soybean protein powder, 2 to 5 parts of glucose, 10 to 15 parts of maltose oligosaccharide and 10 to 20 parts of corn powder. The glucose albumen powder is relatively low in cost and suitable for people of all ages, has no side effect and can improve the physique and enhance the immunity if used for a long time.

The invention relates to balsam pear coarse cereal rice, and in particular relates to a formula of the balsam pear coarse cereal rice. According to the balsam pear coarse cereal rice, mixed powder is formed by coarse cereal powder, balsam pear powder and konjac powder, wherein the balsam pear powder accounts for 6-10% of the mass of the mixed powder; the konjac powder accounts for 0.05-0.3% of the mass of the mixed powder; 100 parts of mixture is formed by uniformly mixing 70-72 parts of the mixed powder and 20-30 parts of drinking water in parts by weight; and the mixture is mechanically processed to be of rice grain shape via extrusion after conditioning. By adopting the technical scheme, the balsam pear coarse cereal rice disclosed by the invention is capable of effectively reducing blood sugar and promoting the metabolism of glucose, protein and fat of a human body and is balanced in nutrition, convenient to cook and good in mouthfeel.

The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.

The invention relates to a cordyceps liquid spawn culture medium, which is mixed and prepared by 30 to 100 gram glucose, 3 to 10 gram peptone, 0.5 to 1.1 gram multiplex vitamin, 5 to 12 gram multiplex mineral matters and 961.5 to 876.9 gram water, wherein, the vitamin mainly comprises VA, VB1, VB2, VB5, VB12, VC and VE which are prepared according to the ratio of 1 to 1; the mineral matters mainly comprise calcium hypochlorite, green vitriol, zinc selenides, sodium chlorides, potassium nitrates, magnesium sulphates, manganese sulphates, ammonia sulphates and potassium iodides which are prepared according to the ratio of 1 to 1 or are mixed and prepared by 2 gram calcium hypochlorite, 0.2 gram green vitriol, 0.2 gram zinc selenides, 0.5 to 1 gram sodium chlorides, 3 gram potassium nitrates, 3 gram magnesium sulphates, 0.2 gram manganese sulphates, 0.3 gram potassium iodides and 2 gram ammonia sulphates. The invention concentrates effective nutrition and medicinal compositions in cordyceps, and reduces the dosage but does not reduce the effect. The cordyceps liquid spawn culture medium can save 90 percent labor and save 90 percent energy, is environment-friendly without refuses; moreover, the seed production technology is simplified, which contributes to the popularity and promotion of cordyceps seed production and culture technologies and is favorable for the development of cordyceps project and simultaneously avoids large-area pollution loss due to various reasons of liquid spawn.

The invention discloses a composition obtained by fermenting folium eriobotryae with probiotics and a preparing method of the composition. The composition is prepared through the steps that folium eriobotryae is dried and smashed into powder, the folium eriobotryae powder is soaked with water, added with glucose, peptone, yeast extract, yeast powder and a sweetening agent, added with inorganic salt, sterilized and then subjected to fermentation treatment with probiotics, polydextrose and oligosaccharide fructose syrup are added, and the composition obtained by fermenting folium eriobotryae with probiotics is obtained. The composition has the effects of removing heat from the lung to relieve coughs, harmonizing the stomach and inducing diuresis, quenching thirst, regulating intestinal flora balance, improving the gastrointestinal function and the like. Compared with a traditional dehairing technology, the technology process is optimized, labor intensity and production cost are reduced, the extraction ratio of effective ingredients of raw materials is greatly increased, the composition can be easily absorbed by the intestinal tract, the utilization rate is increased, and the burden of the intestinal tract of the human body is relieved.

The invention discloses a glycoprotein powder. The glycoprotein powder comprises, by weight, 14-35 parts of organic rice flour, 5-15 parts of calcium lactate, 3-15 parts of glucose, 4-12 parts of composite vitamins, 10-15 parts of galactooligosaccharide, 30-50 parts of peanut powder and 22-36 parts of walnut powder. The glycoprotein powder has the advantages of low cost, suitableness for people of all ages, no side effects, and realization of physique improvement and immunity enhancement through long-term use.

The invention belongs to the technical field of microbiological research and relates to a melanin-producing marine bacterium and a fermentation method for producing natural melanin. The melanin is extracted from the culture product of a marine bacterium. The bacterium needs to be cultured in a medium containing L-tyrosine (L-Tyr) and supplemented with glucose, peptone, beef extract or beef powder and aged seawater, under such a condition that the initial pH value ranges from 6.1 to 7.8. The culture product is subjected to high-speed centrifugation to remove the bacterium, the pH value of the supernatant is adjusted to the range from 2 to 4, the supernatant is incubated at room temperature and then centrifuged to remove foreign matters, and the resulting product is dried until the weight is constant and then eluted with organic solvents to produce the purified melanin (i.e. natural melanin). The bacterium is a strain of Pseudoalteromonas sp. and has a preservation serial number of CGMCC No.1782. The natural melanin has radioprotective and antioxidant effects. The production method has low cost and high yield and can be widely applied.

The invention relates to a new formula of a culture medium used in culture of metarhizium anisopliae which is mainly used for transforming W-oxides to mildew oxides during the biological fermentation process of sterides, and the new formula of the culture medium belongs to the technical field of fermentation of industrial fungi. The new formula of the culture medium is as follows: each 1000ml of the culture medium contains 2.0%-4.5% of glucose by weight, 0.4%-0.7% of peptone by weight, 0.03%-0.07% of magnesium sulfate by weight, 0.2%-0.6% rapeseed oil cake by weight, 0.1%-0.3% prinsepia utilis royle powder by weight, 0.1%-0.3% cotton seed meal by weight and the balance of water. The metarhizium anisopliae cultured by the culture medium is rapid and uniform in growth, and thalli are less prone to balling generally; and in addition, most of raw materials adopted by the culture medium are waste materials from industrial production, the sources are wide, the yield is great, the metarhizium anisopliae can be supplied all the year round, the production cost is low, and the characteristics of low carbon and circulatory production are simultaneously reflected.

The invention discloses a culture medium for improving the active ingredient of Cordyceps sinensis mycelium. The culture medium for improving the active ingredient of Cordyceps sinensis mycelium comprises potato juice, nutritional powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, radix sophorae flavescentis extract, and maifanshi water, wherein the culture medium comprises potato juice, nutritional powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, sophora flavescentis extract, and maifanshi water. Compared with the prior art, the culture medium for improving the active ingredient of Cordyceps sinensis mycelium provided by the invention can shorten the culture period of Cordyceps sinensis, remarkably increase the contents of cordyceps polysaccharide, cordycepic acid, cordycepin and adenosine in the cordyceps sinensis mycelium, and has extremely high nutritional value.

The invention discloses a saccharomyces cerevisiae LTY-1 capable of tolerating the toxicity of lignin. The saccharomyces cerevisiae LTY-1 is preserved in China Center for Type Culture Collection (referred to as CCTCC) on May 6, 2014 and the preservation number is CCTCC NO:M2014184. When a fermentation culture medium which contains 242g/L of glucose, 10g/L of peptone, 61g/L of yeast extract powder and of which the pH value is 5.5 is adopted, the saccharomyces cerevisiae LTY-1 is fermented for 36 hours under the conditions of 30 DEG C and 150rpm and the glucose conversion rate of the saccharomyces cerevisiae LTY-1 is 84.4%. When the fermentation culture medium contains lignin components, such as syringol, p-hydroxybenzaldehyde and vanillin and the concentration of each component is 1.44g/L, the glucose conversion rate of the saccharomyces cerevisiae LTY-1 is only reduced by 7.0% which is far less than 18.5% of the original yeast. The saccharomyces cerevisiae LTY-1 provides the core technology and materials for improving the fermentation efficiency of ethanol in lignocellulosic hydrolyzate. The saccharomyces cerevisiae LTY-1 can be applied in production of bioethanol from lignocelluloses as a feedstock and is of great significance for promoting industrial development of bioethanol production, environmental friendliness and grain safety guarantee.

The invention discloses a bacillus culture medium, which is prepared from the following raw materials: peptone soybean broth, glucose, peptone, sodium dihydrogen phosphate, calcium chloride, biotin and water. According to the bacillus licheniformis culture medium provided by the invention, an OD600nm value of bacillus licheniformis fermentation liquor and the OD600nm value of bacillus subtilis fermentation liquor are respectively increased by 43.3% and 35.1%; the biomass of the bacillus licheniformis/bacillus subtilis is obviously increased; the result shows that the culture medium provided bythe invention is more suitable for the growth of bacillus licheniformis/bacillus subtilis, and the bacillus licheniformis/bacillus subtilis in the optimized culture medium enters a logarithmic phaseafter being fermented for 4 hours, so that the fermentation time can be obviously shortened, and the production cost is greatly reduced.

The invention belongs to the field of microbial fermentation, and specifically relates to a fermentation culture medium for bacillus subtilis, a preparation method for the fermentation culture medium,and a preparation method for a bacillus subtilis preparation. The fermentation culture medium is prepared from 63-74 g/L of bean pulp powder, 82-90 g/L of corn starch, 5-7 g/L of corn steep liquor, 2-3 g/L of a beef extract, 8-12 g/L of peptone, 8-12 g/L of glucose, 2-3 g/L of protease, 2-3 g/L of amylase and 5.73-11.08 g/L of inorganic salt, wherein the enzyme activity of the protease is 1800-2000 U/g; and the enzyme activity of the amylase is 1800-2000 U/g. The fermentation culture medium provided by the invention can provide a nutritional environment applicable to growth of the bacillus subtilis, and the viable count and spore count in a fermentation broth are increased.

The invention provides a fermentation strategy capable of improving toxoflavin production of burkholderia sp.HDXY-02, and belongs to the technical field of microbial fermentation. A strain HDXY-02 ispreserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.14054. The strategy determines that the optimal inoculation amount is 3% and the culture temperature is 30 DEG C. Through single factor experiments and statistical analysis, the optimal culture medium conditions are determined as follows: glucose (19.24 g/L), peptone (20.45 g/L), phenylalanine (0.472 g/L), K2HPO4 (1.5 g/L), MgSO4 (0.75 g/L), and pH 7.0. After optimization, the toxoflavin yield reaches 1533 mg/L and is improved by 296% than that before optimization, and the feasible fermentation strategy is provided for the high production of toxoflavin by a microbial fermentation method.

The invention discloses a probiotic drop for improving allergic constitution and a preparation method of the probiotic drop, and belongs to the technical field of medical biology. The probiotic drop comprises the following raw materials: lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium longum, lactobacillus plantarum, fermented lactic acid bacteria, galactooligosaccharide, xylooligosaccharide, anhydrous glucose, protein hydrolysate, polyhydric alcohols, natural vitamin E, unsaturated fatty acid, vegetable oil and phospholipid oil. The preparation method of the drop is simple, the process is easy to control, and the drop is stable in performance, not solidified at low temperature, uniform in component, good in oxidation resistance and more stable in storage.