228 results about "Esophageal squamous cell carcinoma" patented technology

Quinoline derivatives showing anticancer activities against cancer cell lines of hepatocellular carcinoma (Hep3B), lung carcinoma (A549), esophageal squamous cell carcinoma (HKESC-1, HKESC-4 and KYSE150). The quinoline derivatives have a backbone structure of the following formulas:

Methods of diagnosing whether an epithelial tissue is an abnormal tissue by determining an expression pattern for PML in the epithelial tissue; determining an expression pattern for nuclear bodies in the epithelial tissue; determining SUMO-1 colocalization and comparing the expression pattern for PML and the expression pattern for nuclear bodies with a control are disclosed. Also disclosed are methods for diagnosing whether a subject has mild dysplasia, moderate dysplasia, Type A severe dysplasia, Type B severe dysplasia, cervical squamous cell carcinoma, or poorly-differentiated cervical squamous cell carcinoma, by determining an expression pattern for PML, an expression pattern for nuclear bodies, and SUMO-1 colocalization and determining whether the sample is consistent with expression patterns expected for mild dysplasia, moderate dysplasia, Type A severe dysplasia, Type B severe dysplasia, cervical squamous cell carcinoma, or poorly-differentiated cervical squamous cell carcinoma.

The present invention is a method distinguishing between head and neck squamous cell carcinoma and lung squamous cell carcinoma. In particular, a 10-gene classifier has been identified which can be used to distinguish between primary squamous cell carcinoma of the lung and metastatic head and neck squamous cell carcinoma. These genes include CXCL13, COL6A2, SFTPB, KRT14, TSPYL5, TMP3, KLK10, MMP1, GAS1, and MYH2. A panel of one or more of these genes, or proteins encoded thereby, can be used for early diagnosis and selection of an appropriate therapeutic treatment.

The invention discloses a product for performing assisted prediction on postoperative survival time length of an esophageal squamous carcinoma patient. The product comprises substances for detecting 178 miRNA expression quantity substances comprising hsa-miR-218-5p, hsa-miR-142-3p, hsa-miR-150-5p and hsa-miR-205-5p, and records a carrier with the diagnostic criteria and functional expressions as follows: if 0.94dig is less than dip, the postoperative survival time of the esophageal squamous carcinoma patient to be tested is more than 5 years, and if the 0.94dig is more than or equal to dip, the postoperative survival time of the esophageal squamous carcinoma patient to be tested is less than 5 years. After the result of the postoperative survival time of the esophageal squamous carcinoma patient is predicted by using the product, more accurate and effective treatment is adopted, and the patient condition can be effectively improved. In addition, the medicine which influences the expression quantities of hsa-miR-218-5p, hsa-miR-142-3p, hsa-miR-150-5p and hsa-miR-205-5p can be used for treating the esophageal squamous carcinoma.

The invention belongs to the technical field of biotechnology, and discloses a miRNA combination used for detecting esophageal squamous cell carcinoma and application thereof. The miRNA combination comprises miR-22, miR-127-3p, miR-148b and miR-223, and the miRNA combination can further comprise one or more of miR-10a, miR-100 and miR-133a. A kit comprises a probe combination of the miRNAs. The miRNA combination and the probe combination thereof provided by the invention can be used for detecting the esophageal squamous cell carcinoma, and can be used as a diagnosis marker for improving the accuracy of detection, improving the insuperable low specificity and low sensitivity, caused by individual difference, of a single marker, and improving a clinical detectable rate remarkably by combining other detection indicators and clinical symptoms of a patient, so the miRNA combination and the probe combination thereof become an effective means for early diagnosis of the esophageal squamous cell carcinoma. Due to the adoption of the miRNA combination, a noninvasive serological test is adopted so as to bring convenience to clinical promotion.

The invention relates to an esophageal squamous cell carcinoma autoantibody molecular marker model and an application thereof. The molecular model mainly comprises an ALDOA autoantibody, an ENO1 autoantibody, a p53 autoantibody, and an NY-ESO-1 autoantibody, and can be used for preparing a kit for distinguishing esophageal squamous carcinoma patients and healthy medical examiners. The kit for detecting esophageal squamous cell carcinoma patients mainly comprises recombinant ALDOAD protein, recombinant ENO1 protein, recombinant p53 protein, and recombinant NY-ESO-1 protein. According to the esophageal squamous cell carcinoma autoantibody molecular marker model and the application thereof, the ENO1 autoantibody is found to be elevated in serum level in the esophageal squamous carcinoma patients for the first time, and is jointly detected with the ALDOA autoantibody, the p53 autoantibody, and the NY-ESO-1 autoantibody for distinguishing the esophageal squamous cell carcinoma patients andthe healthy medical examiners, and has a better distinguishing effect than a single index detection; and in addition, the detection method adopted by the invention is an enzyme-linked immunosorbent assay indirect method, is simple and convenient to implement, has good sensitivity and specificity, and is a method which is mature and reliable and can be widely used in base layer hospitals.

The invention discloses a microfluid system used for detecting CTCs of the esophageal squamous cell carcinoma. The microfluid system is prepared through the following steps: 1, preparation of a microfluid pipeline system which is divided into two or more parallel microfluid channels from an inlet, wherein the microfluid channels are provided with a plurality of fishbone structures; 2, etching of a silicon nanowire substrate; 3, surface modification and biotin bio-functionalization of silicon nanowires; and 4, modification of the silicon nanowire substrate and assembling of a PDMS microfluid chip. In use of the system, cells of a to-be-detected sample and 200 [mu]L of a PBS solution of biotin-modified p-EpCAM and p-cKit are subjected to co-incubation for 1 h, and then a co-incubation product is injected into the microfluid system for detection. The microfluid system provided by the invention can recognize and bind to CTCs with extremely low content, and is applicable to traditional immunofluorescence dyeing; obtained detection results and phenotypic analysis results can be subjected to further specific release; so purification of CTCs is realized, and the purity of CTCs is improved so as to meet requirements of gene detection limits.

The invention belongs to the technical field of biotechnology and medical technology, and relates to a marker ALDOA autoantibody for distinguishing between esophageal squamous cell carcinoma patients and normal persons, and a detection method and applications thereof. In the invention, the serum proteome research technology is adopted to identify a new marker ALDOA autoantibody for esophageal squamous cell carcinoma. Purified ALDOA proteins are subjected to ELISA analysis for detecting expression level of the ALDOA autoantibody in serums of esophageal squamous cell carcinoma patients and normal persons, and the results indicate that the level of the ALDOA autoantibody in the serum of esophageal squamous cell carcinoma patients is obviously higher than that of the normal persons. The ALDOA autoantibody of the invention can serve as the marker of the esophageal squamous cell carcinoma and can be used in the diagnosis of the esophageal squamous cell carcinoma.

The invention discloses a kit used for aided prediction on postoperative survival time of esophageal squamous cell carcinoma (ESCC) patients. The kit comprises a substance used for detecting lncRNA 1 expression quantity, a substance used for detecting lncRNA 2 expression quantity, and a substance used for detecting lncRNA 3 expression quantity; the sequence of lncRNA 1 is represented by sequence 1 in a sequence table; the sequence of lncRNA 2 is represented by sequence 2 in the sequence table; and the sequence of lncRNA 3 is represented by sequence 3 in the sequence table. A plurality of ESCC patients are investigated; expression characteristics of lncRNA in cancer tissue and near cancer normal tissue are analyzed; it is observed that expression profile of a plurality of lncRNA in cancer tissue is obviously different from that of near cancer normal tissue; a model is established based on three lncRNA relative expression quantity; and the model can be used for aided prediction on postoperative survival time of esophageal squamous cell carcinoma patients.

The invention relates to an esophageal squamous cell carcinoma primary tumor line CH-H-2 which is used in mammals to produce esophageal squamous cell carcinoma cells. The primary tumor line CH-H-2 can be passed by NOD/SCID mice for multiple times, is significant in solving the inevitable bottleneck problem of distant metastasis in the current esophageal squamous cell carcinoma treatment, and is an ideal object for the basic research and clinical early application of esophageal squamous cell carcinoma.

The invention provides a Lasso-based esophageal squamous cell carcinoma patient risk prediction column diagram model establishment method, which is used for evaluating the postoperative survival risk of esophageal squamous cell carcinoma patients. The method comprises the following steps: firstly, collecting clinical data of esophageal squamous cell carcinoma patients, analyzing the clinical data by utilizing single-factor Cox, Lasso and multi-factor Cox regression analysis methods to obtain important characteristic variables, and establishing probability prediction models with different characteristic dimensions; secondly, selecting a probability prediction model with better performance and establishing a postoperative risk prediction column diagram model of the esophageal squamous cell carcinoma patient; and finally, dividing the patients into a high-risk group and a low-risk group according to the postoperative risk prediction column diagram model of the esophageal squamous cell carcinoma patients, and verifying the reliability and effectiveness of model classification through a KM survival curve analysis method. According to the method, the postoperative survival risk of the esophageal squamous cell carcinoma patient can be accurately predicted, reference is better provided for treatment of the esophageal squamous cell carcinoma patient, and meanwhile the risk prediction cost is reduced.

The invention relates to long non-coding RNA and application thereof. According to a sequence of the long non-coding RNA, specific qRT-PCR (Quantitative Real-time Polymerase Chain Reaction) primers and probes are designed and synthesized and reagents used for esophageal carcinoma auxiliary diagnosis or curative effect prediction are prepared. The qRT-PCR reagents are utilized for detecting the expression level of the long non-coding RNA in specimens of esophageal carcinoma clinical cases and a substantial decrease of the long non-coding RNS expression is found in esophageal carcinoma. The long non-coding RNA can be applied to preparing reagents used for esophageal carcinoma auxiliary diagnosis, curative effect prediction or prognosis judgment.

The invention discloses a medicine for inhibiting proliferation of esophageal cancer cells. The roles of SOX9 in the occurrence and development of esophageal squamous cell carcinoma and a downstream molecular mechanism regulated by the SOX9 are researched from the view of tumor stem cells by detecting clinical specimens and establishing an in vitro cell model and an in vivo mouse model; experiments proves that the SOX9 is highly expressed in the tissues of the esophageal squamous cell carcinoma and in esophageal cancer stem cell balls, and can promote the proliferation and self-renewal of a cell line of the esophageal squamous cell carcinoma, the formation of tumor stem cell balls, the growth of transplantation tumor in nude mice, and the tolerance to chemotherapeutic drug cisplatin. The invention provides a theoretical basis and an experimental basis for further clarifying the occurrence and development mechanism and targeted treatment of esophageal cancer.

The invention belongs to the technical field of biological medicine, provides an application of ZNF750 in screening a targeted medicine for treating esophageal squamous cell carcinoma, provides a novel target for blocking the metastasis of esophageal squamous cell carcinoma cells or inhibiting the change of the esophageal squamous cell carcinoma cells, and provides a treatment clue for patients with esophageal squamous cell carcinoma with ZNF750 variation. The invention provides the application of ZNF750 in screening the targeted medicine for treating the esophageal squamous cell carcinoma. The esophageal squamous cell carcinoma is esophageal squamous cell carcinoma with ZNF750 gene mutation, deletion, inactivation variation or ZNF750 low expression. The targeted medicine treats the esophageal squamous cell carcinoma by inhibiting or blocking angiogenesis-related gene expression of a downstream target gene of ZNF750. The detection of ZNF750 mutation can divide the patients with esophageal squamous cell carcinoma into two groups of mutant type and wild type, and the medicine targeted to KDR can provide effective treatment selection for the patients with ZNF750 inactivation variationand improve the clinical prognosis.

The invention relates to novel application of small molecule metabolites, namely hypoxanthine, 2-ketoisocaproic acid, glutamic acid and aspartic acid, in a serum sample as combined markers in preparation of a kit for distinguishing patients with esophageal squamous carcinoma and esophageal epithelial atypical hyperplasia in subjects. The invention further relates to a kit for detecting patients with esophageal squamous cell carcinoma in subjects. The relative concentration of the combined markers in serum samples from the subjects is detected, the variables P of the combined marker are calculated based on a linear regression equation, and whether the subjects suffer from esophageal squamous cell carcinoma or not is judged based on a determined threshold value. The kit can realize high-sensitivity and high-efficiency detection of several metabolites involved in the invention, and has the advantages of low detection cost and good repeatability. The kit can be applied to clinical auxiliary diagnosis of the esophageal squamous cell carcinoma, has the advantages of high diagnosis sensitivity and capability of effectively distinguishing the esophageal squamous cell carcinoma from precancerous lesions, and has a relatively good application prospect.

The invention relates to an esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof. The molecular model mainly comprises a-SMA+ (invasive frontier) fibroblasts, CD163+ (interstitial) macrophages, CD117+ (protogenic layer) mastocytes and CD117+ (invasion frontier) mastocytes. The molecular model can be used to predict the prognosis of patients withesophageal squamous carcinoma and group adjuvant treatment sensitivity groups. A kit mainly includes an anti-CD117 protein antibody, an anti-CD163 protein antibody and an anti-a-SMA protein antibody.According to the invention, the combination of three molecules combined with histopathological features predicts patients' prognosis, which has a higher predictive effect than single index detectionand clearly distinguishes patients with esophageal squamous carcinoma adjuvant treatment sensitivity; an immune tissue chemical hypersensitivity two-step method on which the molecular model is based is a mature and reliable method which can be widely used in primary hospitals; and compared with international TNM staging, the molecular model has a higher predictive effect, and is simpler, easier, more sensitive and more specific.