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Application of esophageal squamous cell carcinoma primary tumor strain CH-H-1

A technology of CH-H-1 and esophageal squamous cell carcinoma, which is applied in the application field of CH-H-1, a primary tumor strain of esophageal squamous cell carcinoma, to achieve the effects of high metastatic potential and stable traits

Inactive Publication Date: 2012-11-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide an application of a primary tumor strain CH-H-1 of esophageal squamous cell carcinoma, which can be passed down multiple times by NOD / SCID mice. Metastasis, an unavoidable bottleneck problem in the current treatment of esophageal squamous cell carcinoma, is of great significance and is an ideal object for basic research and preclinical application of esophageal squamous cell carcinoma

Method used

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  • Application of esophageal squamous cell carcinoma primary tumor strain CH-H-1
  • Application of esophageal squamous cell carcinoma primary tumor strain CH-H-1
  • Application of esophageal squamous cell carcinoma primary tumor strain CH-H-1

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Embodiment 1

[0027] 1. Establishment of primary esophageal squamous cell carcinoma xenograft tumor lines

[0028] (1) Experimental animals: NOD / SCID mice, 4-6 weeks old, male or female, were purchased from the Experimental Animal Center of Second Military Medical University.

[0029] (2) Tumor tissue: Fresh esophageal tumor tissue specimens were obtained from a 53-year-old male patient with stage IIIa esophageal squamous cell carcinoma who underwent surgery in the Department of Thoracic and Cardiovascular Surgery, Changhai Hospital Affiliated to Second Military Medical University in January 2007.

[0030] (3) Tumor formation and passage expansion of primary tumor xenograft:

[0031] ①NOD / SCID mice were pretreated with cobalt-60-γ-ray irradiation 1 day before inoculation, and the irradiation dose was 2.5Gy / mouse.

[0032] ②The mice were anesthetized by intraperitoneal injection of pentobarbital sodium (0.05mg / g body weight), and the primary esophageal squamous cell carcinoma tissue was cut...

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Abstract

The invention relates to an application of an esophageal squamous cell carcinoma primary tumor strain CH-H-1 in a mammal for producing esophageal squamous cell carcinoma cells. The primary tumor strain CH-H-1 disclosed by the invention can realize multiple passages through NOD / SCID mice, has significance for solving the bottleneck that the current treatment of the esophageal squamous cell carcinoma can not avoid distant metastasis, and is an ideal object for fundamental research on the esophageal squamous cell carcinoma and clinical early application.

Description

technical field [0001] The invention belongs to the field of primary tumor xenograft tumor models, and particularly relates to the application of a primary tumor strain CH-H-1 of esophageal squamous cell carcinoma. Background technique [0002] For a long time, all preclinical studies of human tumors have been limited to in vitro culture of tumor cells or in vivo studies of tumor-bearing model animals. In practice, it has been found that most human tumor cells cannot survive for a long time after breaking away from the original living environment in vivo, and only a small number of tumor cells that have been screened by the environment can grow in vitro for a long time (World Cancer report2008.IARC Press , Lyon, 2008). Since George Gey first established HeLa cell line, the first human cervical squamous cell line that can be passaged in vitro in 1952, the tumor cell line model has been widely used by experts in the industry because of its long-term stable passage in vitro an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
Inventor 黄盛东李白翎袁扬龚德军陶婧
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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