Heatproof lyoprotectant for live vaccine against pseudorabies and preparation method thereof, and lyophilized vaccine and preparation method thereof
A heat-resistant freeze-drying and protective agent technology, applied in the preparation of freeze-dried vaccines and freeze-dried vaccines, heat-resistant freeze-dried protective agents for live pseudorabies vaccines, and the preparation of protective agents, can solve the problem of insufficient protective effects of heat-resistant protective agents to improve dehydration protection, promote sublimation, and improve tolerance
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Embodiment 1
[0026] A heat-resistant freeze-drying protectant for pseudorabies live vaccine, comprising the following components by weight: 30g of gelatin, 10g of trehalose, 50g of sucrose, 1g of bovine serum albumin, 10g of tryptone, 20g of enzymolyzed casein, 10g of thiourea, L-sodium glutamate 8g, arginine 1g, polyvinylpyrrolidone (PVP-K30) 5g and mannitol 1g.
[0027] The preparation method of above-mentioned pseudorabies live vaccine heat-resistant lyoprotectant comprises the following steps:
[0028] (1) Preparation of liquid A
[0029] Weigh trehalose, bovine serum albumin, enzymatic casein, sodium L-glutamate, thiourea, arginine, polyvinylpyrrolidone and mannitol according to the formula, add an appropriate amount of water for injection until completely dissolved, and then make up to volume to 1L, and finally filter and sterilize with a 0.22μm filter membrane;
[0030] (2) Preparation of liquid B
[0031] Weigh gelatin, sucrose and tryptone according to the formula, dissolve in ...
Embodiment 2
[0035] A heat-resistant freeze-drying protectant for pseudorabies live vaccine, comprising the following components by weight: 100g of gelatin, 50g of trehalose, 150g of sucrose, 20g of bovine serum albumin, 80g of tryptone, 100g of enzymolyzed casein, 50g of thiourea, L-sodium glutamate 20g, arginine 30g, polyvinylpyrrolidone (PVP-K30) 50g and mannitol 20g.
[0036] The preparation method of above-mentioned pseudorabies live vaccine heat-resistant lyoprotectant comprises the following steps:
[0037] (1) Preparation of liquid A
[0038] Weigh trehalose, bovine serum albumin, enzymatic casein, sodium L-glutamate, thiourea, arginine, polyvinylpyrrolidone and mannitol according to the formula, add an appropriate amount of water for injection until completely dissolved, and then make up to volume to 1L, and finally filter and sterilize with a 0.22μm filter membrane;
[0039] (2) Preparation of liquid B
[0040] Weigh gelatin, sucrose and tryptone according to the formula, diss...
Embodiment 3
[0044] A heat-resistant freeze-drying protectant for pseudorabies live vaccine, comprising the following components in parts by weight: 50g of gelatin, 15g of trehalose, 96g of sucrose, 2g of bovine serum albumin, 40g of tryptone, 40g of enzymatic hydrolyzed casein, and 16g of thiourea , L-sodium glutamate 12g, arginine 2g, polyvinylpyrrolidone (PVP-K30) 10g and mannitol 1g.
[0045] The preparation method of above-mentioned pseudorabies live vaccine heat-resistant lyoprotectant comprises the following steps:
[0046] (1) Preparation of liquid A
[0047] Weigh trehalose, bovine serum albumin, enzymatic casein, sodium L-glutamate, thiourea, arginine, polyvinylpyrrolidone and mannitol according to the formula, add an appropriate amount of water for injection until completely dissolved, and then make up to volume to 1L, and finally filter and sterilize with a 0.22μm filter membrane;
[0048] (2) Preparation of liquid B
[0049] Weigh gelatin, sucrose and tryptone according to ...
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