The present invention rationally designs and prepares a new 2,4-dinitrobenzenesulfonyl modified polycarbonate polymer-PMPC-Dns to detect selenocysteine in a living body. The compound can encapsulate the fluorescent drug doxorubicin (DOX) and selectively respond to Sec and selenol compounds without interference from biothiols, amines, and alcohols. We investigated micelle formation, Sec-response, probe cytotoxicity, and Sec-responsive drug release. PMPC‑Dns micellar probes can be applied to imaging endogenous Sec in living cervical cancer tissues and HeLa cells under physiological conditions, and PMPC‑Dns micellar probes can simultaneously image and release drugs encapsulated in them. This work opens a way to understand the role of Sec in physiological, pathological systems, and tumor xenograft model systems, and also provides a method for the controlled release of hydrophobic molecules among target molecules for biomedical applications.