The invention discloses a drug sensitivity kit, a preparing method thereof and a bacterium drug sensitivity detection method. The drug sensitivity kit comprises a box body, a culture medium slab, scraps of paper of drug sensitivity and a testing apparatus, the box body is internally provided with an accommodation cavity, the culture medium slab is arranged in the accommodation cavity and comprisesa common nutrient agar slab, a MacConkey agar slab, an SS agar slab and a tryptic soytone broth agar slab containing a coenzyme A, the scraps of paper of drug sensitivity are arranged in the accommodation cavity, and each scrap of paper of drug sensitivity correspondingly contains any one of tulathromycin, acetyl-isovaleryl tulathromycin, valnemulin, levofloxacin, lomefloxacin, ciprofloxacin, enrofloxacin, gentamicin, kanamycin, streptomycin, tylosin, tilmicosin, spectinomycin, florfenicol, ceftiofur, tiamulin, doxycycline, colistin, amoxicillin and lincomycin. The drug sensitivity kit has the advantages that the testing result is precise, the consumed time is short, the operation is simple, and the cost is low.