The invention discloses an extraction-free reagent kit for detecting gene polymorphism of human APOE and SLCO1B1. The reagent kit comprises main reaction liquor, ApoE primer and probe mixed liquor 1,ApoE primer and probe mixed liquor 2, SLCO1B1 primer and probe mixed liquor 1, SLCO1B1 primer and probe mixed liquor 2, positive quality control and feminine quality control, wherein the main reactionliquor comprises DNA polymerase, four kinds of deoxyribonucleotide, Mg2+, Tris buffer liquor and ultrapure BSA, and the DNA polymerase includes TTX enzymes and/or HS-D-Taq enzymes. The reagent kit can directly complete test on whole blood samples and swab samples through a unique amplification system, and a primer and probe sequence and system taking account of specificity and sensitivity, the detection is completed in four reaction system closed pipes, so that pollution can be avoided; and in addition, the feminine quality control and the positive quality control in the reagent kit can effectively monitor false positivity and validity of reagents and operations, and can effectively control pollution and monitor the whole experiment process.