Kit for detecting polymorphism of APOE gene and SLCO1B1 gene

Abstract

The invention provides a kit for detecting polymorphism of an APOE gene and an SLCO1B1 gene. The kit comprises primers used for detecting polymorphism sites T388C and C526T of the APOE gene and expressed as SEQ ID NO. 1-6, primers used for detecting polymorphism sites A388G and T521C of the SLCO1B1 gene and expressed as SEQ ID NO. 7-12, and internal quality control housekeeping gene primers expressed as SEQ ID NO. 13-20. The kit is used for carrying out fluorescence PCR by using ARMS-PCR and fluorescence fusion curve techniques, is capable of achieving accurate detection of one polymorphism oftwo genes in the same reaction tube, is high in sensitivity and high in specificity, and can meet detection of oral epithelial cells, dried-blood spots and whole-blood samples; the whole detection process is short in time; the medication basis can be provided for the doctor in the first time; the medication risks of the patients can be reduced.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a detection kit for APOE and SLCO1B1 gene polymorphism. Background technique [0002] The APOE gene is a gene encoding human apolipoprotein E (apolipoprotein E, APOE). This gene is located on chromosome 19p13 and has 4 exons and 3 introns. The APOE protein encoded by it belongs to the apolipoprotein family. It is an important part of plasma lipoprotein, mainly expressed in liver and brain tissue, participates in the body's lipid metabolism through various channels, and is an important internal factor affecting blood lipid levels. [0003] Studies have shown that the APOE gene has nucleotide polymorphisms, and the two most common single nucleotide polymorphisms are APOE T388C (rs429358) and APOE C526T (rs7412), forming three haploids, respectively For E2 (388T-526T), E3 (388T-526C), E4 (388C-526C) three protein phenotypes, including three homozygous (E2 / E2, E3 / E3, E4 / E4) and thre...

AI Technical Summary

Problems solved by technology

Among them, the most common method is the sequencing method, which is relatively cheap, but it takes a long time to operate and has low sensitivity; the restriction fragment length polymorphism analysis method has low detection sensitivity and cumbersome operation steps, and the detection results still need to be verified by the next-generation sequencing method. Re-validation, especially when the sample size is large, it is easy to cause cross-contamination of PCR products and prone to false negative or false positive results due to insufficient or excessive digestion; high-resolution melting curve method has special requirements for equipment There are certain difficulties in popularization; the accuracy and repeatability of the detection results of chip detection are poor, and the experimental cycle is long; the traditional fluorescent quantitative PCR method is widely used in clinical practice, but this method is generally used to detect gene polymorphisms. Genotyping method Detection, this method has certain requirements on the number of samples and the distribution of polymorphisms. When the sample size is small, it cannot accurately detect the genetic polymorphisms of the samples.

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