8 results about "Polymorphism analysis" patented technology

The invention discloses a method for accurately identifying and screening morchella strains by using molecular markers, and belongs to the technical field of edible mushrooms. The method comprises the following steps: transferring a purified morchella strain to a culture medium for dark light cultivation; adding the cultured morchella hyphae into liquid nitrogen, grinding into powder, then adding a lysis solution, and carrying out lysis; adding modified magnetic beads into the lysate for adsorption, and then filtering, washing and eluting to obtain a DNA extracting solution; carrying out PCR (Polymerase Chain Reaction) amplification on the DNA; then carrying out high-resolution detection on an ISSR product; and then, carrying out functional SNP-KASP typing. According to the method, by fusing ISSR polymorphism analysis and a functional SNP-KASP typing technology, magnetic beads are used for adsorbing DNA to remove polysaccharide interference, and on the basis that the low cost advantage of traditional ISSR is reserved, three functional markers are completed, and accurate screening of yield, heat resistance and flavor is achieved.

The invention discloses an oryzias chinensis SSR molecular marker primer group and application, and belongs to the technical field of molecular biology. The oryzias chinensis SSR molecular marker primer group provided by the invention comprises 10 pairs of primers, and the primer sequences of the primers are sequentially shown as SEQ ID NO.1-20. The oryzias chinensis SSR molecular marker primer group can be used for polymorphism analysis of an oryzias chinensis population in combination with a PCR amplification technology, and a basis is provided for construction of a genetic structure and a genetic map of the oryzias chinensis population.

This invention relates to the field of marker-assisted breeding technology, and in particular to a molecular marker related to the muscle quality traits, amino acid content, and fatty acid content of beef cattle, and its application. Through sequencing and polymorphism analysis of 106 Yanhuang cattle, this invention identified an SNP site located in exon 2 of the CRYAB gene. Based on this, a molecular marker was developed. This marker not only enables rapid and accurate typing of the CRYAB gene in beef cattle (such as Yanhuang cattle), but also shows a significant correlation with individual meat quality traits, amino acid content, and fatty acid content. Based on the genotype of the molecular marker, individuals with higher overall meat quality traits can be screened at an early stage, thereby improving the speed of breeding superior beef cattle.

The application discloses a kind of predictable phaseolus vulgaris response melatonin regulation SSR molecular markers and its application, it is related to biotechnology field.The application carries out the effectiveness identification of ordinary phaseolus vulgaris natural germplasm resources to melatonin treatment, the efficiency of melatonin treatment-cell wall-salt tolerance in ordinary phaseolus vulgaris germplasm resources is analyzed, and using the reference genome in Esembl plants database, a complete set of SSR molecular markers is developed, and according to transcriptome information, the SSR marker near response gene is mined, through polymorphism analysis and single marker analysis, two SSR markers with significant difference associated with melatonin traits are screened, can be used as the molecular marker for predicting phaseolus vulgaris response melatonin regulation, and provides technical support for the function prediction of melatonin treatment in the sprouting stage of ordinary phaseolus vulgaris.

The present invention relates to a method for determining the risk of developing Korean-specific metabolic syndrome by genetic polymorphism analysis, and specifically, to a method for determining single nucleotide polymorphism (SNP) from a biological sample, a biomarker composition for determining metabolic syndrome in Koreans including single nucleotide polymorphism (SNP), and a composition for predicting the risk of developing metabolic syndrome, comprising an agent capable of detecting the biomarker composition. According to the present invention, useful information can be provided for predicting the risk of developing metabolic syndrome in Koreans based on genetic polymorphism analysis.

This invention discloses a KASP primer for synergistically regulating wheat thousand-grain weight and SDS-sedimentation value, and its application. Based on the polymorphism analysis of candidate genes, this invention precisely locates a functional single nucleotide polymorphism (SNP) site that simultaneously positively regulates the two core traits mentioned above, and develops a high-throughput molecular diagnostic tool. Validation in natural populations shows that this site exhibits strong pleiotropic effects: materials carrying the HAP I haplotype not only show excellent thousand-grain weight but also a significant simultaneous increase in SDS-sedimentation value. This detection system possesses advantages such as high throughput, low cost, and digital genotyping, overcoming the technical bottleneck of traditional quality identification being limited by large quantities of grains and the difficulty in simultaneously considering yield and quality. It achieves early and accurate diagnosis of "dual-superior" genotypes in wheat, which is of great significance for molecular-directed polymerization breeding of high-yielding, strong-gluten wheat.

For molecular genetic breeding of crops, molecular markers for identifying an allele at a rice-blast-resistant Pik locus of rice and use thereof are provided. The Pik gene is re-sequenced, and through sequence polymorphism analysis, identity codes, 3 SNPs, composed of nucleotide types at reverse sites 806, 901 and 4210 of the Pik-1 start codon have been found, which effectively identify Pik, Pike, Pil, Pikm, and Piks. The molecular markers quickly screen the allele type at the Pik locus, obtain an accurate result just by PCR (Polymerase Chain Reaction) and electrophoresis detection, and has high throughput and low cost.

The application belongs to the technical field of genetic engineering, and particularly relates to an InDel molecular marker for distinguishing wheat haplotype and / or identifying wheat with high thousand kernel weight and application thereof. The application takes 332 hexaploid wheat varieties as research objects, performs polymorphism analysis, finds that Hap2 compared with Hap1, there is one InDel: -475bp contains 67bp base deletion, which is named InDel-541 / -475, and the deleted nucleotide sequence is specifically shown as SEQ ID NO. 1. Based on the site, Hap1 and Hap2 can be distinguished, and wheat varieties with high thousand kernel weight can be identified. Based on the InDel molecular marker of the application, a primer is designed, and only simple PCR specific amplification is needed to distinguish the wheat haplotype, complete the identification of wheat with high thousand kernel weight, facilitate the detection and screening of wheat varieties or lines with high thousand kernel weight, and can greatly accelerate the breeding process of wheat varieties with high thousand kernel weight.