79 results about "Polymorphism analysis" patented technology

The invention discloses a method for directly detecting miRNA in an absolute quantification mode, and belongs to the field of molecular biology. The method adopts an miRFLP measurement method and includes the steps that a sample to be detected and a dynamic miRNA standard material are mixed uniformly, miRNA reverse transcription, cDNA tailing, PCR synchronous amplification and fluorescence fragment length polymorphism analysis of a PCR amplification product are carried out, in the step of cDNA tailing, the tail end of an adaptive oligonucleotide chain 3' is modified, reaction background signals are reduced, and interference of homologous sequences at the tail end of miRNA 3' is avoided; a biotin-agarose streptomycin coupling reagent or streptomycin magnetic beads are adopted for enrichment of reverse transcription and PCR reactants, the loading quantity of the sample is increased, method errors are reduced, germ RNA is added as a protective reagent, and measurement errors are reduced. The sample of 0.4 microliter can be directly measured, and at the measurement level of 128 molecules, the changing range of measurement is reduced to 9.9%. The measurement sensitivity is remarkably improved, and the measurement error range is narrowed substantially.