A method and a kit for detecting nucleic acid sample pollution and application of the method
A nucleic acid sample and kit technology, applied in the field of nucleic acid sample contamination detection, can solve problems such as cross-contamination and sample contamination
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[0050] 1. Screening of SNP loci
[0051] Not all SNP sites are suitable for the detection of immune repertoire contamination samples. First, it must be ensured that the primers for amplifying the SNP sites, the multiple PCR primers of the immune repertoire, and the CDR3 region of BCR / TCR have no more than 4 bp contiguousness. Base identical or complementary pairing, to avoid the impact of SNP site detection on the multiple PCR amplification of immune repertoire.
[0052] In this example, the primers of the existing 21 SNP sites were first compared, analyzed and screened, and then the primers screened out by the comparative analysis were screened through specific tests to use specific SNP site primers as SNP site primers for this example. sample detection method. The sequence comparison analysis in this example uses the sequence comparison software independently developed by BGI, or uses sequence comparison software such as DNAman. The primers for the 21 SNP sites used for scr...
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