A method and a kit for detecting nucleic acid sample pollution and application of the method

A nucleic acid sample and kit technology, applied in the field of nucleic acid sample contamination detection, can solve problems such as cross-contamination and sample contamination

Active Publication Date: 2018-11-16
BGI GENOMICS CO LTD +2
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Problems solved by technology

[0005] However, in the process of sample transportation or library construction, different degrees of sample contamination or cross-contamination will inevitably occur, whi...

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  • A method and a kit for detecting nucleic acid sample pollution and application of the method
  • A method and a kit for detecting nucleic acid sample pollution and application of the method
  • A method and a kit for detecting nucleic acid sample pollution and application of the method

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Embodiment

[0050] 1. Screening of SNP loci

[0051] Not all SNP sites are suitable for the detection of immune repertoire contamination samples. First, it must be ensured that the primers for amplifying the SNP sites, the multiple PCR primers of the immune repertoire, and the CDR3 region of BCR / TCR have no more than 4 bp contiguousness. Base identical or complementary pairing, to avoid the impact of SNP site detection on the multiple PCR amplification of immune repertoire.

[0052] In this example, the primers of the existing 21 SNP sites were first compared, analyzed and screened, and then the primers screened out by the comparative analysis were screened through specific tests to use specific SNP site primers as SNP site primers for this example. sample detection method. The sequence comparison analysis in this example uses the sequence comparison software independently developed by BGI, or uses sequence comparison software such as DNAman. The primers for the 21 SNP sites used for scr...

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Abstract

The application discloses a method and a kit for detecting nucleic acid sample pollution and application of the method. The method for detecting the nucleic acid sample pollution includes the following steps: PCR amplification is performed on a nucleic acid sample to be measured by adopting a SNP detection primer, mononucleotide polymorphism of the nucleic acid sample to be measured is analyzed, whether pollution of different individual sources is existed in the nucleic acid sample to be measured or not is judged according to a mononucleotide polymorphism analysis result; and the SNP detectionprimer is composed of at least an analysis primer pair of 14 SNP sites. The specificity of each individual SNP is creatively utilized and is used as specific identification information of the nucleicacid sample to be detected, if SNP information of the detected nucleic acid sample to be measured conforms to the self SNP information, the result that the nucleic acid sample to be detected is not polluted is judged, if not, and the result that the nucleic acid sample to be detected has pollution is judged. Whether the nucleic acid sample to be measured exists pollution or not can be accuratelyjudged by the detection method.

Description

technical field [0001] The present application relates to the field of detection of nucleic acid samples, in particular to a method, kit and application for detecting contamination of nucleic acid samples. Background technique [0002] Nucleic acid samples are the basis of genome research. If there is contamination in nucleic acid samples, it will directly affect genome sequencing and subsequent functional research. Therefore, ensuring that nucleic acid samples are not contaminated is a prerequisite for genome research. In order to avoid contamination of nucleic acid samples, the existing common practice is to strictly control the testing process from sampling to nucleic acid extraction, to subsequent sequencing; Preparation of test solution was carried out on the ultra-clean workbench. However, these are only control measures taken in the early stage, and there is no effective detection or test monitoring method for specific tests or whether nucleic acid samples are actua...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869
CPCC12Q1/6869C12Q2531/113C12Q2537/143C12Q2535/122
Inventor 陈冬菊李红梅董燕吴海燕马媛马俊平叶明芝朱红梅
Owner BGI GENOMICS CO LTD
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