145 results about "Specific identification" patented technology

The first aspect of the invention relates to a bispecific antibody, which comprises a first functional domain for specific identification of phosphatidylinositol protein polysaccharide-3, a second domain for specific identification of human T cell antigen CD3, and a connection for connecting the functional domains. The second aspect of the invention relates to a nucleotide sequence encoding the above antibody. The third aspect of the invention relates to a carrier containing the above nucleotide sequence, and includes an expressive vector. The fourth aspect of the invention relates to a eukaryotic or prokaryotic expression system containing the above carrier. The fifth aspect of the invention relates to application of the above antibody to preparation of medicament for treating or preventing tumor.

The invention discloses a matrix metalloproteinase-2 specific multi-modality molecular image probe preparation method and application thereof. The preparation method comprises the following steps: 1)preparing peptide substrates for matrix metalloproteinase-2(MMP-2) specific identification; 2) modifying near infrared fluorescent dye on peptide substrate; 3) modifying fluorescent quenching group onpeptide substrate; 4) connecting different molecular weight PEG or tumor targeting group RGD on peptide substrate modified with near infrared fluorescent dye and fluorescent quenching group; 5) labeling the nuclide on the above modified peptide substrate side chain tyrosine. Compared with the prior art, the matrix metalloproteinase-2 specific multi-modality molecular image probe preparation method has the advantages that: 1) by utilizing the specificity and responsiveness of the MMP-2 protease by the probe, so that the probe is selectively enriched at the tumor site so as to improve the targeting property of the probe to the tumor; 2) performing multi-modality imaging in a living body tumor by using advanced molecular imaging technology such as optical, opto-acoustic, SPECT and the like,improving the sensitivity and accuracy of tumor imaging, and finally achieving accurate positioning of the tumor; 3) providing a new train of thought and method for early diagnosis, process research and prognosis evaluation of tumor.

The invention discloses a QCM detection method for detecting lysozyme based on multiple signal amplification technologies and application. The QCM detection method comprises the following steps that DNA hybridizes with lysozyme aptamer partially in a complementary mode, and through the specific binding reaction of the lysozyme and the lysozyme aptamer, the DNA is released; a Y-shaped structure is formed by complementary hybrid of the released DNA with hairpin DNA and assistant DNA modified on a gold leaf, and under the action of restriction enzyme, the hairpin DNA is cut and opened through specific identification sites; under the action of DNA ligase and DNA polymerase, using locking-ring-shaped DNA as a template chain, polymerization growing along the opened hairpin DNA is carried out, and a single chain with a large number of repeated sequences is formed; a signal probe marked with biotin hybridizes with the generated repeated sequences in a complementary mode, and after binding with streptavidin marked by HRP, hydrogen peroxide is catalyzed to oxidize 4-chloro naphthol, and precipitation reaction is generated; and accordingly the chip surface quality is increased, and the high-sensitivity detection of the QCM to the lysozyme is realized.

The invention discloses an enzyme linked immunosorbent assay kit for detecting a diethoxy organophosphorus pesticide based on a nano antibody. The enzyme linked immunosorbent assay kit contains the nano antibody as a detection antibody, and an amino acid sequence of the nano antibody is shown as SEQ ID NO.1. The kit is the enzyme linked immunosorbent assay kit, and the enzyme linked immunosorbentassay kit comprises an enzyme label plate containing organophosphorus pesticide antigen, a horseradish peroxidase-labelled secondary antibody solution, a diethoxy organophosphorus pesticide standard solution, substrate liquid, a substrate buffer solution, a stopping solution, and condensation washing liquid. The expression level of the diethoxy organophosphorus pesticide based on the nano antibodyis high, the stability is strong, the kit can be used for detecting a plurality of the diethoxy organophosphorus pesticides, realizes broad spectrum specific identification of the diethoxy organophosphorus pesticide, has the advantages of accurate detection result, good effect, good stability, simple operation, high sensitivity, and low cost, and can accurately detect the residues of the diethoxyorganophosphorus pesticide in an agricultural product.

The invention discloses a method for identifying squids or highly processed product varieties thereof by virtue of fluorescent quantitative PCR. The method comprises the following steps: (1) extracting genome DNA from a to-be-detected squid sample; (2) designing specific identification primer and probe for common squids, wherein the four common squids include Ommastrephes bartramii, dosidicus gigas, illex argentinus and todarodes pacificus; (3) setting a fluorescent quantitative PCR amplification system; and conducting real-time fluorescent PCR detection by virtue of the specific primer and probe by taking the genome DNA as an amplification template; and (4) judging in accordance with the result of the real-time fluorescent PCR detection. By virtue of the method disclosed by the invention, the squids and the highly processed product varieties thereof can be rapidly identified.

The invention relates to a fast ultra-sensitive LM (listeria monocytogene) detector based on nanochannel confinement characteristics and constructed according to the nature of specific identification between a target molecule and an aptamer of the target molecules. The invention further relates to a method for detecting the LM by taking potassium ferricyanide ions as probe ions and taking an LM DNA modified porous alumina membrane as an electrode for assembling a self-made electrolytic tank. When LM is present and the concentration is lower, a current increasing value is remarkably reduced with the increase of the concentration; a current change value is reduced with increase of the LM concentration; when the LM concentration is in a range of 100-1,250 CFU/mL, a linear relation is formed between the LM concentration and the current increasing value; when the LM concentration is higher than 1,500 CFU/mL, the current change value becomes stable. Therefore, the lowest detection limit of the detection method for the LM can reach 100 CFU/mL, the linear range is 100-1,250 CFU/mL, and the detection can be completed within 10 min; a 108 CFU/mL of escherichia coli and staphylococcus aureus control experiment indicates that the method has high selectivity on the LM.

The invention discloses a fluorescent probe for detection of cystatin C and a construction method thereof, relates to the molecular biological and microbiological technical fields, and is characterized in that the construction method comprises the steps: with Cys-C as a target, screening to obtain a Cys-C specific-binding recombinant phage by using a phage surface random displayed 12-mer peptide library, extracting single stranded DNA of the recombinant phage, carrying out sequencing and sequence comparison, to obtain a sequence Gln-Val-Asn-Gly-Leu-Gly-Glu-Arg-Ser-Gln-Gln-Met of a Cys-C specific affinity ligand having the molecular weight of 1346.5, then carrying out solid phase polypeptide synthesis and fluorescence labeling to obtain the fluorescent probe FITC-Acp-Gln-Val-Asn-Gly-Leu-Gly-Glu-Arg-Ser-Gln-Gln-Met having the molecular weight of 1848.7 and specifically bond with the Cys-C. The fluorescent probe and the construction method thereof have the beneficial effects: 1, a brand new 'tool' is provided for specific identification and content detection of the Cys-C; and 2, the fluorescent ligand peptide probe not only can qualitatively identify the Cys-C, but also can quantitatively detect the content of the Cys-C in samples.

The invention relates to a molecularly imprinted photonic crystal film for rapidly detecting lysozyme and belongs to the field of material chemistry and analysis and detection. The molecularly imprinted photonic crystal film is characterized in that the molecularly imprinted photonic crystal film which can realize specific identification and binding to lysozyme protein molecules and has the inverse opal structure is prepared by combining silica photonic crystal microspheres with the molecular imprinting technology. As the number of the bound lysozyme protein molecules increases, the macroporous structure on the film changes, the Bragg diffraction wavelength of the prepared molecularly imprinted photonic crystal film is made to realize red shift. According to the spectral response of the prepared molecularly imprinted photonic crystal gel membrane to the lysozyme, rapid detection of the concentration of the lysozyme in the sample can be realized. The molecularly imprinted photonic crystal film is advantaged in that properties of high sensitivity and selectivity and low detection limit are realized, and the molecularly imprinted photonic crystal film can be used as a biosensor used for rapidly detecting the lysozyme protein molecules in a biological sample.

The invention relates to the technical field of pharmaceutical analysis, in particular to a burned Rhizoma drynariae formula granule characteristic spectrum and an establishing method thereof. The establishing method includes that octadecylsilane chemically bonded silica is used as a filling agent, methanol is used as a mobile phase A, a 0.1v/v% phosphoric acid solution is used as a mobile phase B, and gradient elution is performed according to regulations in the following table; detection wavelength is 290 nm, column temperature is 20 DEG C, and flow speed is 1.0ml/min; number of theoretical plates should not be lower than 50000 if being calculated according to naringin peak; six characteristic peaks should be presented in a test piece characteristic spectrum, and relative retention time should be within +/-5% of specified values; the specified values are 0.299 (peak 1), 0.446(peak 2), 0.557(peak 3), 0.579(peak 4), 0.911(peak 5) and 1.00(peak 6). According to characteristics of formula granules, specific identification and multi-component integral quality control are enhanced, and the characteristic spectrum of the variety is established for comprehensive quality control.

Belonging to the technical field of traditional Chinese medicine, the invention relates to identification methods of Chinese herbal medicines, in particular to a specific molecular marker for panax ginseng and panax quinquefolius and an identification method thereof. The invention provides a DNA molecular marker for specific identification of panax ginseng and panax quinquefolius. The DNA molecular marker is located on the (SSR) loci of DNA sequences Seq.No.1-5 respectively. 5 pairs of primers are designed according to the sequences to perform PCR (polymerase chain reaction) amplification on the DNA templates of panax ginseng and panax quinquefolius. PCR products for specific identification of panax ginseng and panax quinquefolius can be amplified respectively by each pair of the primers, the products with different lengths are detected by polyacrylamide gel electrophoresis and agarose gel electrophoresis, so that panax ginseng and panax quinquefolius can be rapidly identified. The method needs a small amount of samples, is not restricted by product shapes, and has low requirement for sample's DNA length. The PCR is stable and has good repeatability.

The invention belongs to the field of cell and gene engineering, genetic modification and therapeutic recombinant protein industrial production, and particularly relates to GS (glutamine synthetase) gene specific identification crRNA and application thereof. DNA (deoxyribonucleic acid) sequences identified by the GS gene specific identification crRNA are selectively DNA sequences shown as one of sequences SEQ ID NO.1-SEQ ID NO.8. The GS gene specific identification crRNA and the application have the advantages that GS genes of diversified cells can be specifically identified by the GS gene specific identification crRNA, the GS gene specific identification crRNA is wide in applicability, and integral procedures can be implemented easily and efficiently; the target protein expression quantities of cells without the GS genes can be greatly increased on the basis of imported exogenous carriers as compared with wild host cells, and accordingly required-to-be-inputted labor, material resources and financial resources for screening protein expression cell strains can be reduced; the GS genes are knocked out, and accordingly the industrial production cost can be reduced; the cells without the GS are used as host target gene expression cells, other chemical substances can be omitted in procedures for producing the cells, and accordingly the production safety can be improved.

The invention firstly provides an RPA (Recombinase Ploymerase Amplification) detection method of identification of transgenic insect-resistant rice kefeng No.6 strain and in particular discloses a primer and probe combination used for indentifying transgenic insect-resistant rice kefeng No.6 via a recombinase ploymerase amplification technology; a sequence of a positive primer is expressed as SEQ ID No.1; a sequence of a negative primer is expressed as SEQ ID No. 2; a sequence of the probe is expressed as SEQ ID No.3. The invention also discloses a method for indentifying transgenic insect-resistant rice kefeng No.6. The method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of a sample to be detected as a template, performing RPA quick amplification and real-time fluorescence detection by using the primer; and proving that the detected sample contains components of the transgenic insect-resistant rice kefeng No.6 if an obvious amplification curve is obtained.

The invention discloses sugarcane genome endogenous reference gene acetolactate synthase gene PCR (polymerase chain reaction) primer sequences and an amplification method. The primer sequences are composed of ScALS-F and ScALS-R. The PCR amplification method comprises a reaction system and amplification conditions. The invention provides a genome endogenous reference gene for PCR detection and species specific identification of transgenic sugarcane, and meanwhile, provides insurance for detection precision and result accuracy of the transgenic sugarcane.

The invention relates to a small RNA detection method and a use thereof and belongs to the field of double-stranded small RNA detection. The small RNA detection method comprises the following steps of 1, designing multiple continuous or discontinuous closed RNAs which are complementary with one chain of a small RNA, a reverse transcription stem-loop primer which is complementary with an end 3' of the other chain of the small RNA, and a forward primer and a reverse primer for quantitative PCR, and 2, detecting the small RNA by denaturation, enclosing, inverse transcription and quantitative PCR. The small RNA detection method has simple processes, a low detection cost, a detection limit reaching a femtogram grade, high detection efficiency, strong singularity and high sensitivity. The small RNA detection method can be widely used for quantitative analysis, qualitative analysis and pharmacokinetic analysis of a small RNA drug, for amplification, specific identification and differentiation of a small RNA molecule, and especially for molecular diagnosis, small RNA drug metabolism and small RNA drug functional study.

The application discloses a method and a kit for detecting nucleic acid sample pollution and application of the method. The method for detecting the nucleic acid sample pollution includes the following steps: PCR amplification is performed on a nucleic acid sample to be measured by adopting a SNP detection primer, mononucleotide polymorphism of the nucleic acid sample to be measured is analyzed, whether pollution of different individual sources is existed in the nucleic acid sample to be measured or not is judged according to a mononucleotide polymorphism analysis result; and the SNP detectionprimer is composed of at least an analysis primer pair of 14 SNP sites. The specificity of each individual SNP is creatively utilized and is used as specific identification information of the nucleicacid sample to be detected, if SNP information of the detected nucleic acid sample to be measured conforms to the self SNP information, the result that the nucleic acid sample to be detected is not polluted is judged, if not, and the result that the nucleic acid sample to be detected has pollution is judged. Whether the nucleic acid sample to be measured exists pollution or not can be accuratelyjudged by the detection method.

The invention provides an electrochemical AC impedance biosensor for detecting MMP-14 and a preparation method thereof. The polypeptide inhibitor is dissolved in the Tris-HCl buffer solution so as toobtain the MMP-14 specific identification probe solution, wherein that amino acid sequence of the polypeptide inhibitor is: GYPKSALR-(CH2) 6-Cys; the gold electrode is immersed into the MMP-14 specific identification probe solution to perform self-assembling modification and then rinsed by the Tris-HCl buffer solution so as to obtain the IVSC/GE electrode; and the MCH solution is dropped into theIVSC/GE electrode surface for sealing and the electrode surface is rinsed so as to obtain the electrochemical AC impedance biosensor for detecting MMP-14. The electrochemical AC impedance biosensor has high specificity.

The invention provides a group of real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primers for distinguishing a clade 7.2 type H5 AIV (H5 Subtype Avian Influenza Virus) from a clade 2.3.4.4 type H5 AIV, and the real-time fluorescent quantitative PCR primers are designed by utilizing GC content difference existing in characteristic nucleotide sequences of HA (Hemagglutin) genes of the clade 7.2 type H5 AIV and the clade 2.3.4.4 type H5 AIV. The clade 7.2 type H5 AIV and the clade 2.3.4.4 type H5 AIV are effectively distinguished by utilizing the phenomena that both the real-time fluorescent quantitative PCR primers can be effectively amplified when real-time fluorescent quantitative PCR is carried out on the clade 7.2 type H5 AIV and the clade 2.3.4.4 type H5 AIV, but the real-time fluorescent quantitative PCR primers have melting temperature (Tm value) difference existing in melting curves generated after the real-time fluorescent quantitative PCR is carried out on the clade 7.2 type H5 AIV and the clade 2.3.4.4 type H5 AIV. By matching with a computer which is connected with a real-time fluorescent quantitative PCR instrument and utilizing analysis software of the real-time fluorescent quantitative PCR instrument, specific identification and diagnosis on infection situations of the clade 7.2 type H5 AIV and the clade 2.3.4.4 type H5 AIV can be achieved by just one group of primers. According to the real-time fluorescent quantitative PCR primers provided by the invention, an identification method is simple, and the efficiency and an accuracy rate are higher.

The invention discloses a characteristic sequence, specific identification primer and identification method for identifying crassostrea sikamea. The characteristic sequence is located in a mitochondrial non-coding region between glycine tRNA synthetase and proline tRNA synthetase of the crassostrea sikamea, and the characteristic sequence is a nucleotide sequence as shown in SEQ ID NO.1; a sequence of the specific identification primer comprises an upstream primer MNR-F: 5' -CTGTAAGTATATTTGTCTTCCA-3' ; and a downstream specific primer MNR-S-R: 5' -AGGCTTTCACTCCACTTACT-3' . According to the invention, the crassostrea sikamea can be effectively distinguished from other four drassostrea species, the length of the determined characteristic sequence of the crassostrea sikamea is 660+/-5 bp, and whether the crassostrea sikamea exists or not can be judged according to the existence and the size of a specific band. In the field of crassostrea sikamea germplasm resource protection and crassostrea sikamea breeding, species identification can be conducted on parent crassostrea sikamea before seed breeding, and it is guaranteed that bred seeds are purebreds and are not affected by other species possibly hybridized with the bred seeds.