47 results about "Specific identification" patented technology

The invention discloses an immature bitter orange-based original plant identification method based on an InDel molecular marker and a special primer combination, and belongs to the technical field of traditional Chinese medicinal material identification. In order to solve the problem of uncontrollable quality of medicinal materials caused by miscellaneous immature bitter orange base (lime, sweet orange and Yichang orange) in the prior art, two pairs of specific primers YCC-F / R (SEQ ID NO: 1-2) are screened out through genome re-sequencing and are used for specific identification of the Yichang orange (amplifying a single bande-1000bp); the invention relates to a specific primer for identifying three primary plants, in particular to a specific primer for identifying three primary plants, namely SC-F / R (SEQ ID NO: 3-4), which is used for distinguishing acid orange (single bande-1300 bp) and sweet orange (double bande-1200 bp / 1300 bp), and triple accurate identification of the three primary plants can be realized by combining PCR (Polymerase Chain Reaction) amplification and gel electrophoresis analysis. The method is simple and convenient to operate, low in cost, short in period and suitable for large-scale source tracing and quality control of medicinal materials.

The invention relates to the technical field of quality detection, and provides a method for detecting the quality of an aminobutyric acid extract in peanut sprouts, which comprises the following steps: performing specific recognition on aminobutyric acid by a molecular imprinting sensor, and calculating the impurity contribution by combining a preset peanut sprout interference database and partial least square regression; the interference of complex matrix impurities in the peanut bud extract can be specifically eliminated, and the problem that the specificity is insufficient when a traditional method is used for detecting a complex sample is solved; the molecularly imprinted sensor and the quartz crystal microbalance are connected in series through the microfluidic channel, and resonance frequency detection is triggered by using capacitance variation, so that a coherent process is formed from identification to quality analysis of sample detection, and the detection efficiency is improved; the corrected frequency offset, the phase angle and the temperature are input into the recurrent neural network, accurate output of the concentration of the aminobutyric acid is achieved by integrating multi-dimensional detection information, compared with a single parameter detection method, the real content of the aminobutyric acid in an extract can be reflected more comprehensively, and a reliable basis is provided for quality judgment.

The invention discloses a controllable nanorod array SERS (Surface Enhanced Raman Scattering) chip-based digital detection device for a serous ovarian cancer exosome phenotype as well as a detection method and application thereof, and relates to the technical field of biological detection. The device comprises a controllable nanorod array module, a high-sensitivity SERS probe module, an integrated microfluidic platform module and a digital analysis module. The controllable nanorod array module is used for accurately capturing a single exosome; the SERS probe module realizes specific recognition of multiple biomarkers on the surface of the exosome by conjugating a specific antibody and a Raman reporter molecule; the micro-fluidic platform module integrates filtering, reaction and imaging functions, so that high-throughput and automatic detection is realized; and the digital analysis module completes qualitative and quantitative analysis of exosome phenotypes. The problems that a traditional detection technology cannot analyze the phenotype difference of single exosomes, the sensitivity is low and the flux is insufficient are solved, early accurate diagnosis of the serous ovarian cancer can be achieved, and the kit has wide clinical application prospects.

The application discloses a temperature-sensitive molecular imprinting sensitive film, a preparation method thereof and application thereof in a glyphosate detection sensor, and belongs to the field of optical sensing technology and environmental monitoring. The application forms an imprinting cavity which is highly complementary to a template molecule in size and spatial configuration in a polymer network, realizes specific identification of a target molecule, and solves the problems of complex sample pretreatment and long detection period of the existing glyphosate detection method. The application introduces a temperature-sensitive material PNIPAM, realizes in-situ, non-destructive and reversible release of the template molecule by temperature control, discards the traditional destructive elution mode, avoids causing serious secondary environmental pollution, avoids the problems of swelling and corrosion of the imprinting cavity caused by strong acid or organic solvent, and causes irreversible damage to the structure of the imprinting cavity, which seriously affects the reuse frequency of the sensor and the detection stability.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The invention belongs to the technical field of bioengineering, and discloses a specific identification primer and method for lactobacillus curvatus in human intestinal tracts and application. The identification primer comprises an upstream primer COG3825F1 and a downstream primer COG3825R1, the nucleotide sequence of the upstream primer COG3825F1 is 5 '-GTCACAACAAGACCGATTT-3', and the nucleotide sequence of the downstream primer COG3825R1 is 5 '-CTGTTTCTACAACTTGTGCT-3', and the nucleotide sequence of the upstream primer COG3825F1 and the nucleotide sequence of the downstream primer COG3825R1 are 5 '-GTCACAACAAGACCGATTT-3'. The specific COG3825 gene of the lactobacillus flexus is screened to serve as a target sequence design primer, and PCR, gel electrophoresis and qPCR verification show that the primer has extremely high specificity and sensitivity and can accurately distinguish the lactobacillus flexus from the related lactobacillus and other common intestinal bacteria under the complex micro-ecological background of the human intestinal tract; the identification method based on the primer is easy and convenient to operate and accurate in result, qualitative and quantitative detection of the lactobacillus brevis in human intestinal samples, food samples and other environment samples can be achieved, and important technical support is provided for related research, application development and micro-ecological monitoring of the lactobacillus brevis.

This invention belongs to the field of microbial technology and relates to a thermoresistant, high-esterase-producing *Changfangluode* yeast and its specific identification method. The high-esterase-producing, thermoresistant *Changfangluode* yeast (… Lodderomyces elongisporus FBKL2.9-G53, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20253046 on December 29, 2025, exhibits excellent thermostability, maintaining good growth activity and enzyme production capacity even at 45℃. Quantitative esterase analysis showed a significant thermo-enhancing effect on enzyme production, with esterase activity of 12.83 U / mL at 28℃, increasing to 14.87 U / mL at 40℃ and 20.56 U / mL at 45℃. Simulated fermentation experiments further confirmed that the strain demonstrated robust metabolic adaptability under 45℃ heat stress, with esterase activity reaching 7.44±0.35 U / mL on day 7 of fermentation, approximately 1.55 times higher than the control group at 28℃.

The present application provides a DNA barcode specific identification method of radix rehmanniae decoction pieces, and relates to the technical field of true and false identification of traditional Chinese medicine decoction pieces. The specific identification method comprises the following steps: extracting sample DNA to be detected; screening universal and specific DNA barcode genes; amplifying universal and specific DNA barcode gene fragments; amplifying the amplification product; and obtaining amplified DNA barcode sequences after sequence correction and splicing, and performing online BLAST comparison with NCBI database, so as to determine the base origin of the sample to be detected. The method provided by the present application can overcome the problem that the DNA barcode of the conventional ITS2 sequence of the decoction piece after processing is not easy to amplify, and the PCR amplification product of the present application has high quality, the molecular identification result is accurate, and the rapid identification of radix rehmanniae and radix rehmanniae preparata decoction pieces can be efficiently and high-quality solved, and the urgent need for true and false identification of radix rehmanniae decoction pieces during acceptance is solved.

The present invention relates to the field of bioengineering technology, and in particular to a flanking sequence of the rice GAT transformation event GATV3-470-2 and a specific identification method thereof. The right flank sequence of the flanking sequence is shown as SEQ ID NO.1; the left flank sequence of the flanking sequence is shown as SEQ ID NO.2. The present invention provides a set of specific primers for the flanking sequence of the rice transformation event GATV3-470-2, including nucleotide sequences shown as SEQ ID NO.11-12 and SEQ ID NO.9-10, which can be used to detect components derived from the rice transformation event GATV3-470-2 in biological materials. The flanking sequence of the rice transformation event GATV3-470-2 and the corresponding detection primers provided by the present invention can be used for the detection of components derived from the rice transformation event GATV3-470-2, which is of great significance in the field of biosafety assessment of transgenic rice and its derivative lines.

The invention discloses a genome editing and detecting system for recognizing a DNA target based on TDP-43 dimerization and application of the genome editing and detecting system, and belongs to the technical field of molecular biology. FokT fusion protein construction, cell transfection and functional labeling, FokT-Seq detection through high-throughput sequencing, target annotation and functional analysis and disease mutation experience verification are carried out, and the genome editing and detecting system for recognizing the DNA target based on TDP-43 dimerization is obtained. Specific recognition and functional marking on the TDP-43 dimerization dependent DNA binding site are achieved for the first time, and traditional ChIP-Seq, CUTamp, CCTP-Seq, CCTP-Seq, CCTP-Seq, CCTP-Seq; a conformational state monomer or dimer when TDP-43 is combined with DNA cannot be judged by methods such as FOkI, FOkI is only activated in a dimerization state, and only a DNA target of TDP-43 combined in a dimer form is marked, so that the structure specificity of recognition is remarkably improved, and false positive binding site interference is avoided.

The application belongs to the field of chemical detection, and relates to a Schiff base copper ion colorimetric fluorescent probe molecule and a preparation method and application thereof. The structural formula of the Schiff base copper ion colorimetric fluorescent probe molecule is: the preparation method has simple synthesis steps, and only needs to pass through one-step reaction, and has high yield. The fluorescent probe provided by the application exhibits high efficient and specific identification performance on copper ions in a PBS buffer solution system, has the advantages of good selectivity, strong anti-interference ability, fast response speed (response can be completed within 30 seconds), low detection limit (18 nM), and a wide pH value application range; colorimetric experiment results show that the probe can realize naked-eye detection on copper ions, and has wide application prospects in the fields of public safety, environmental monitoring and the like.

The invention discloses a primer group, a kit and a method for identifying deer species based on IGF1 (Insulin-like Growth Factor 1), IGF2 (Insulin-like Growth Factor 2) and RAF1 genes. The primer group comprises three pairs of specific primers aiming at IGF1, IGF2 and RAF1 genes, and the sequences of the three pairs of specific primers are respectively SEQ ID NO: 12 and 13, SEQ ID NO: 14 and 15, and SEQ ID NO: 16 and 17. In specific identification, DNA of a sample is extracted, PCR amplification is carried out by using the primer group, and then the fragment length difference of an amplification product is analyzed through agarose gel electrophoresis. By comparing specific length combinations of IGF1, IGF2 and RAF1 gene amplified fragments, the four types of cervidae animals can be accurately distinguished. According to the application, the identification system for the cervidae species based on the IGF1, IGF2 and RAF1 genes is established for the first time, and the identification system has the advantages of rapidness, accuracy, low cost and no need of sequencing, and is suitable for species source identification and market supervision of related products.

This application relates to the field of quality testing technology and provides a method for quality testing of GABA extract in peanut sprouts. It utilizes a molecularly imprinted sensor for specific identification of GABA, combined with a pre-set peanut sprout interference database and partial least squares regression to calculate impurity contribution. This method can specifically eliminate interference from complex matrix impurities in peanut sprout extracts, solving the problem of insufficient specificity in traditional methods for complex samples. The molecularly imprinted sensor is connected in series with a quartz crystal microbalance via a microfluidic channel. Capacitance changes trigger resonant frequency detection, creating a coherent process from identification to quality analysis and improving detection efficiency. By inputting corrected frequency offset, phase angle, and temperature into a recurrent neural network, the method integrates multi-dimensional detection information to achieve accurate output of GABA concentration. Compared to single-parameter detection methods, this method more comprehensively reflects the true content of GABA in the extract, providing a reliable basis for quality judgment.

The application discloses a multiplex PCR specific identification primer for a pheasant meat source component, and the nucleotide sequence of a pheasant specific primer is 5'-GTAAAGTAGGACCATGCCTCT-3'. The application further discloses a kit containing the multiplex PCR specific identification primer, and a multiplex PCR identification method for the pheasant meat source component. The pheasant specific primer provided by the application can perform PCR amplification on 12S rRNA, the amplification effect is stable, a 151bp specific band can be generated, and the pheasant meat source can be effectively identified.

The present application belongs to the field of biotechnology, and particularly relates to a biomarker for differential diagnosis of recent and long-term tuberculosis infection. The present application determines the differential proteins in blood of Mycobacterium tuberculosis recent infection and long-term infection through proteomics, and provides a diagnostic biomarker for differential diagnosis of Mycobacterium tuberculosis recent infection and long-term infection. The biomarker provided by the present application has good diagnostic efficiency whether it is used for single diagnosis or combined diagnosis, and the AUC value is greater than or close to 0.8. The biomarker can be used for more rapid, efficient, sensitive and specific identification of Mycobacterium tuberculosis recent infection, and provides a diagnostic basis for early intervention of tuberculosis.

This invention discloses a high-throughput quality control method for Qingfei Paidu Granules. Based on liquid chromatography-mass spectrometry, this method establishes a comprehensive quality control method for Qingfei Paidu Granules. This method enables specific identification of the 19 ingredients in the Qingfei Paidu Granules, limit testing of the toxic component aristolochic acid I, fingerprint analysis, and content determination of four indicator components in a single analysis, significantly improving detection efficiency and reducing testing costs. This method enables rapid, comprehensive, and accurate quality assessment of Qingfei Paidu Granules, providing technical support for the development of new Qingfei Paidu Granules.

The invention discloses application of a nanochannel membrane in specific recognition of L-Glu in a nanofluid device, and belongs to the technical field of membrane science. The nanofluid device comprises two conductance cells and an L-glutamate oxidase (GLOD) modified HGO-COOH / TOCNF nanochannel membrane arranged between the two conductance cells, electrodes are placed in the two conductance cells respectively, and the two conductance cells are filled with salt solutions with the same concentration; according to the application, after the GLOD modified HGO-COOH / TOCNF nano-channel film is soaked in liquid to be detected, volt-ampere testing is carried out, and recognition detection data of L-Glu is obtained according to the current response condition. The nano-channel membrane has good ion transmission stability when being used for a nano-fluid device, is high in recognition sensitivity and good in stability when being used for specifically recognizing L-Glu, and has considerable application value.

This invention relates to the field of biodetection technology, specifically to a primer set for identifying Fritillaria taibaiensis, a real-time quantitative PCR detection kit, and their applications. The primer set includes a first ARMS primer pair, a second ARMS primer pair, and a universal primer pair for the Fritillaria genus. The second ARMS primer introduces an artificial mismatched base near the 3' end, improving the specific recognition ability of the target site and reducing the risk of false negatives due to rare variations in the target region. Based on real-time quantitative PCR amplification using specific fluorescent probes, the relative quantitative analysis of Fritillaria taibaiensis DNA in the sample is achieved through the Ct value of the amplification curve. Furthermore, combined with amplification using universal primers for the Fritillaria genus and HRM analysis, the melting peak characteristics of the amplified product are used to determine whether other Fritillaria species are mixed in the sample, thereby achieving specific identification, relative quantitative analysis, and adulteration identification of Fritillaria taibaiensis, improving the accuracy and reliability of Fritillaria-type medicinal materials identification.

The present invention discloses a fluorescent carbon quantum dot, a preparation method and an application thereof, and relates to the technical field of quantum dot materials. The raw materials of the fluorescent carbon quantum dots of the present invention include terephthalaldehyde and thiourea. The fluorescent carbon quantum dots of the present invention use terephthalaldehyde and thiourea as raw materials, have aggregation-induced fluorescence enhancement characteristics, and the preparation method is simple, easy to operate, low in cost, green and mild. The fluorescent carbon quantum dots can be quenched by p-nitrophenol as a fluorescent probe. Therefore, they can be used for rapid, sensitive and highly specific identification of p-nitrophenol, overcoming the shortcomings of existing organic pollutant detection methods such as long detection time, complicated steps and high cost.

The invention discloses a sulfamethoxazole molecular imprinting ratio fluorescent paper-based sensor based on a rare earth complex fluorescent material as well as a preparation method and application of the sulfamethoxazole molecular imprinting ratio fluorescent paper-based sensor. The sulfamethoxazole molecularly imprinted ratiometric fluorescent paper-based sensor is prepared by the following steps: carrying out polymerization reaction on a red fluorescent material BCP-Eu, a green fluorescent material BCP-Tb, a template molecule sulfamethoxazole, a functional monomer and a cross-linking agent APTES on the surface of a paper base, eluting to remove the sulfamethoxazole template molecule, and detecting the sulfamethoxazole molecularly imprinted ratiometric fluorescent paper-based sensor. Finally, the sulfamethoxazole molecular imprinting ratio fluorescent paper-based sensor is obtained. According to the paper-based sensor, a molecular imprinting technology and a ratio fluorescence analysis method are organically combined, the paper-based sensor has the characteristics of high selectivity and high sensitivity, and specific recognition, rapid quantitative analysis and visual detection of sulfamethoxazole can be realized by further combining convenient imaging and signal reading functions of a smart phone; and an effective solution is provided for on-site instant detection of food safety.

The application discloses Macrobrachium rosenbergii 5K liquid phase chip and application thereof, relates to the technical field of molecular detection of aquatic species resources, and comprises a target capture probe combination and a 6087 specific SNP site combination; the SNP sites are all located on a Macrobrachium rosenbergii reference genome HiCasm202009.chrome.fasta, and the probe combination is used for specific identification and enrichment detection of the SNP sites. Through the use of strictly screened high-quality sites, specifically designed specific probes according to target sequences and liquid phase targeted hybridization enrichment technology, efficient and accurate detection without redundant data is realized, the stability and reliability of the typing result are ensured, the detection and analysis cost is reduced, the chip can comprehensively support the multiple applications of Macrobrachium rosenbergii genotyping, species identification, pedigree reconstruction and good seed breeding, and the efficiency and standardization of Macrobrachium rosenbergii species management and precision breeding are improved.

The application provides application of a CNN2-B probe in specific identification of an atherosclerotic plaque, and relates to the technical field of fluorescent probes. ‑ The structural formula of the CNN2-B probe is as follows: the CNN2-B probe is an ONOO ‑ / lipid double response type probe, which has very weak fluorescence in the case of existing alone, can only make fluorescence sharply increase in the case of existing of lipid and ONOO ‑ , and has lipid droplet positioning function. The probe can be used in specific identification of an atherosclerotic plaque, so that the position of the atherosclerotic plaque can be more accurately judged.

The invention belongs to the field of biotechnology detection, and discloses a fluorescent quantitative PCR (polymerase chain reaction) primer probe composition for pseudorasbora parva and application. A specific upstream primer, a specific downstream primer and a TaqMan probe are designed according to a COI gene sequence in a mitochondrial DNA sequence of the pseudorasbora parva, the primer is obtained through comparison and screening, the primer has the characteristic of high specificity, the fluorescent quantitative PCR method established based on the primer can efficiently, accurately and specifically identify the pseudorasbora parva through genome DNA, the influence of subjective factors is eliminated, and the primer is high in sensitivity and high in specificity. The repeatability is good; and the method can be used for quantitatively analyzing the eDNA of the pseudorasbora parva, so that the pseudorasbora parva can be efficiently, accurately and specifically identified without damaging the pseudorasbora parva, and technical support can be provided for monitoring, protecting and managing population resources of the pseudorasbora parva which are specifically distributed in narrow areas in China.

The invention relates to a Chinese cattle multi-variety identification SNP (Single Nucleotide Polymorphism) marker combination and a liquid phase chip technology. According to the present invention, the genetic characteristics of the five Chinese cattle varieties and the 38 varieties of the six districts are deeply analyzed, the specific identification sites are screened, the unique gene identification of each variety can be accurately identified, the liquid phase chip is further developed, and the chip has characteristics of rich functional sites, good pertinence and good accuracy, can be used for variety identification and specific character identification, and can be used for identifying the variety and the specific character. Low-cost and high-throughput detection is realized, and powerful support is provided for identification, protection and development of Chinese cattle varieties.

The invention discloses a bimodal sensor as well as a preparation method and application thereof. The bimodal sensor comprises Fe3O4 (at) TCPP, wherein the surface of the Fe3O4 (at) TCPP is modified with a mercury ion aptamer; the Fe3O4 (at) TCPP comprises Fe3O4 and tetra (4-carboxyphenyl) porphin, and the tetra (4-carboxyphenyl) porphin is coated on the surface of the Fe3O4. The nanoprobe has low cost and good anti-interference and selectivity, can realize specific recognition of mercury ions in food, further realizes fluorescence / colorimetric spectrum bimodal detection result output, achieves the purpose of self-correction detection, and improves the selectivity, accuracy and efficiency of detection.

The label comprises a base layer and a scrapable layer, the base layer is arranged on the outer surface of a water bottle body, the scrapable layer covers the surface of the base layer and completely covers the surface of the base layer, an identification layout is arranged on the base layer and divides the surface of the base layer into a plurality of checks, and the checks are arranged on the surface of the base layer. Each grid is endowed with a specific identification symbol, the scrapable layer is a plant starch-based composite film, the thickness of the scrapable layer is 0.03-0.05 mm, the diameter of each degradable colored short fiber is 0.1 mm, the length of a single degradable colored short fiber is 2-3 mm, six degradable colored short fibers are distributed per square centimeter, and the color of the degradable colored short fibers is selected from at least one of fluorescent orange, sapphire blue and grass green. The method has the beneficial effects that a unique identifier combination is formed by utilizing random physical characteristics generated by the scraping action of the user and the identifier layout arranged on the base layer, and a more intuitive and convenient identifier memory mode is provided for the user.

The utility model relates to the technical field of isothermal amplification detection, in particular to a centrifugal tube adaptive to specific recognition based on colors, which comprises a first cover body, a first tube body, a second tube body and a third tube body which are sequentially arranged from top to bottom, the outer wall of the second pipe body is further sleeved with a second cover body. The first cover body is connected with the first pipe body; the first cover body is in threaded connection with the second pipe body; the second cover body is in threaded connection with the third pipe body; two discharge holes which are communicated with the two cavities in the first pipe body are formed in the side wall of the first pipe body at different heights; and the height of each discharge hole is matched with the height of the third pipe body and the threaded connection height of the first cover body and the second pipe body. The device is simple in structure and low in cost; the reaction substrate can be added without opening a cover, so that the leakage risk is avoided, and the waste of adding tools can be reduced.

The present invention discloses a single-base mutation imaging method based on in situ ARMS-PCR and Cas labeling. This method belongs to the field of single-cell analysis. By adding base mismatch sites to PCR primers, effective amplification and specific identification of single nucleotide variation information within bacteria are achieved. At the same time, the amplified products are labeled by modified gRNA / Cas12a. This method can also accurately quantify Salmonella resistant bacteria in complex environments and obtain cellular heterogeneity and spatial location information.

This invention discloses a norfloxacin nucleic acid aptamer OUC-NOR7-2T and its application, belonging to the field of nucleic acid aptamer technology. The nucleotide sequence of the norfloxacin nucleic acid aptamer OUC-NOR7-2T is shown in SEQ ID NO.10. The application of the norfloxacin nucleic acid aptamer OUC-NOR7-2T in the identification or detection of norfloxacin is also discussed. The norfloxacin nucleic acid aptamer OUC-NOR7-2T of this invention was obtained through screening, exhibiting an affinity dissociation constant of 70.9 nM with norfloxacin. It shows no significant affinity for structural analogs of norfloxacin such as enrofloxacin, ciprofloxacin, ofloxacin, lomefloxacin, and pefloxacin, and can be used for the specific identification or detection of norfloxacin.