83 results about "Specific identification" patented technology

The invention discloses a reversible background-free hydrogel nanosensor for acetone detection and an intelligent sensing platform, and relates to the technical field of biosensors. The hydrogel nanosensor consists of a hydrogel matrix and an upconversion nanoprobe, and the upconversion nanoprobe comprises upconversion nanoparticles (UCNPs), hydroxylamine sulfate and thymol blue (TB). The invention adopts hydroxylamine sulfate as a specific recognition unit of acetone to construct a reversible luminescent hydrogel probe for acetone detection, and the probe can be reused in multiple detections, thereby reducing the cost of a single detection. In addition, by adopting near-infrared light as an excitation light source, the autofluorescence interference of the hydrogel matrix is ​​effectively avoided, thereby improving the detection accuracy.

The invention provides a primer group for qualitative and quantitative detection of three types of fish toxicity karenia, a detection method and application, and belongs to the technical field of marine aquaculture guarantee. The primers as shown in SEQ ID NO.1-6 provided by the invention can be used for respectively identifying the species of the Karenia saddle, the Karenia longgroove and the Karenia butterfly; a plasmid standard curve and a cell standard curve are established in combination with an SYBR Green I fluorescent quantitative PCR technology, and specific identification and quantity determination of the three types of fish-toxic karenia are further realized. The method has the advantages of high specificity, high sensitivity, simplicity in operation, short time consumption and the like, can overcome the limitation of traditional morphological identification, can realize rapid and accurate qualitative identification and quantitative detection of a large number of samples, is time-saving and labor-saving, is relatively low in price, and can become an effective technical means for prediction, early warning and large-area monitoring of the Karenia red tide.

The present invention discloses a SNP molecular marker combination of poplar, a whole-genome liquid chip prepared therefrom, and applications thereof. The SNP molecular marker combination of poplar includes 60,944 SNP molecular markers, and the physical positions of the 60,944 SNP molecular markers are determined by sequence alignment based on the poplar reference genome GCA_015852605.2. The SNP molecular markers of poplar in the present invention can be used for specific identification of five major sections of poplar species. Using the SNP molecular markers of poplar to prepare a whole-genome liquid chip for poplar tree species can achieve efficient, accurate genotyping detection of low-cost poplar germplasm resource populations, and is specific to the sections of Populus, such as Leuce, Aigeiros, Tacamahaca, Leucoides, and Turanga, and has important application values in multiple fields of poplar biological breeding.

The invention discloses an immature bitter orange-based original plant identification method based on an InDel molecular marker and a special primer combination, and belongs to the technical field of traditional Chinese medicinal material identification. In order to solve the problem of uncontrollable quality of medicinal materials caused by miscellaneous immature bitter orange base (lime, sweet orange and Yichang orange) in the prior art, two pairs of specific primers YCC-F / R (SEQ ID NO: 1-2) are screened out through genome re-sequencing and are used for specific identification of the Yichang orange (amplifying a single bande-1000bp); the invention relates to a specific primer for identifying three primary plants, in particular to a specific primer for identifying three primary plants, namely SC-F / R (SEQ ID NO: 3-4), which is used for distinguishing acid orange (single bande-1300 bp) and sweet orange (double bande-1200 bp / 1300 bp), and triple accurate identification of the three primary plants can be realized by combining PCR (Polymerase Chain Reaction) amplification and gel electrophoresis analysis. The method is simple and convenient to operate, low in cost, short in period and suitable for large-scale source tracing and quality control of medicinal materials.

The invention discloses a visual detection method of Seneca valley virus based on CRISPR-Cas13d (clustered regularly interspaced short palindromic repeats-associated protein 13d), and belongs to the technical field of molecular biology diagnosis. The method comprises the following steps: firstly, amplifying a target virus sequence by utilizing an isothermal amplification technology RAA, specifically identifying and amplifying signals by utilizing a CRISPR-Cas13d system, specifically identifying and amplifying signals by utilizing a crRNA sequence as shown in SEQ ID NO.13, and finally, visually observing a detection result by utilizing various visualization methods, according to the detection method provided by the invention, the SVA virus can be accurately and quickly detected in time on site, the detection capability of the SVA virus is remarkably improved, and the biological safety risk is reduced.

The invention relates to the technical field of quality detection, and provides a method for detecting the quality of an aminobutyric acid extract in peanut sprouts, which comprises the following steps: performing specific recognition on aminobutyric acid by a molecular imprinting sensor, and calculating the impurity contribution by combining a preset peanut sprout interference database and partial least square regression; the interference of complex matrix impurities in the peanut bud extract can be specifically eliminated, and the problem that the specificity is insufficient when a traditional method is used for detecting a complex sample is solved; the molecularly imprinted sensor and the quartz crystal microbalance are connected in series through the microfluidic channel, and resonance frequency detection is triggered by using capacitance variation, so that a coherent process is formed from identification to quality analysis of sample detection, and the detection efficiency is improved; the corrected frequency offset, the phase angle and the temperature are input into the recurrent neural network, accurate output of the concentration of the aminobutyric acid is achieved by integrating multi-dimensional detection information, compared with a single parameter detection method, the real content of the aminobutyric acid in an extract can be reflected more comprehensively, and a reliable basis is provided for quality judgment.

The invention provides a construction method of a specific chromatogram of pinus tabulaeformis nodule formula granules, the specific chromatogram and application of the specific chromatogram. The construction method comprises the following steps: (1) preparing the pinus tabulaeformis nodule formula granules into a test solution; preparing the pinus tabulaeformis knot reference medicinal material into a reference medicinal material reference solution; preparing a silver pinocembrin monomethyl ether reference substance into a reference substance solution; and (2) respectively carrying out ultra-high performance liquid chromatography detection on the test solution, the reference substance solution of the reference medicinal material and the reference substance solution of the reference substance, and obtaining the specific chromatogram of the pinus tabulaeformis knot formula granules according to detection. According to the specific chromatogram constructed by the method provided by the invention, the specific identification of the pinus tabulaeformis formula granules is enhanced, the pinus tabulaeformis medicinal material base can be effectively identified, and a support is provided for ensuring the singleness of the source of the pinus tabulaeformis formula granules medicinal material base. The specific chromatogram constructed by the invention can comprehensively reflect the characteristic peak information of the sample, and the method is stable, high in precision and better in reproducibility.

The invention relates to the technical field of biology, in particular to a cotton rat species specific primer designed based on a mitochondrial D-loop region. The specific primer comprises a forward primer, a reverse primer, a forward primer, a reverse primer and a reverse primer, wherein the forward primer is GGACATAGGAAGGAAG; and a reverse primer: TAACTATAAGGCTAGGACC. The kit is characterized in that the kit is used for detecting the disease resistance of the human The specific primer provided by the invention can be specifically combined with a target sequence of a cotton rat mitochondrial D-loop region, a DNA fragment of the region is amplified through a PCR reaction, the fragment covers the complete D-loop region of a cotton rat mitochondrial genome, and species specificity identification of cotton rats can be realized through sequencing and sequence comparison. Meanwhile, a detection product for identifying cotton rat species can be prepared based on the primer sequence.

The invention discloses a controllable nanorod array SERS (Surface Enhanced Raman Scattering) chip-based digital detection device for a serous ovarian cancer exosome phenotype as well as a detection method and application thereof, and relates to the technical field of biological detection. The device comprises a controllable nanorod array module, a high-sensitivity SERS probe module, an integrated microfluidic platform module and a digital analysis module. The controllable nanorod array module is used for accurately capturing a single exosome; the SERS probe module realizes specific recognition of multiple biomarkers on the surface of the exosome by conjugating a specific antibody and a Raman reporter molecule; the micro-fluidic platform module integrates filtering, reaction and imaging functions, so that high-throughput and automatic detection is realized; and the digital analysis module completes qualitative and quantitative analysis of exosome phenotypes. The problems that a traditional detection technology cannot analyze the phenotype difference of single exosomes, the sensitivity is low and the flux is insufficient are solved, early accurate diagnosis of the serous ovarian cancer can be achieved, and the kit has wide clinical application prospects.

The application discloses a temperature-sensitive molecular imprinting sensitive film, a preparation method thereof and application thereof in a glyphosate detection sensor, and belongs to the field of optical sensing technology and environmental monitoring. The application forms an imprinting cavity which is highly complementary to a template molecule in size and spatial configuration in a polymer network, realizes specific identification of a target molecule, and solves the problems of complex sample pretreatment and long detection period of the existing glyphosate detection method. The application introduces a temperature-sensitive material PNIPAM, realizes in-situ, non-destructive and reversible release of the template molecule by temperature control, discards the traditional destructive elution mode, avoids causing serious secondary environmental pollution, avoids the problems of swelling and corrosion of the imprinting cavity caused by strong acid or organic solvent, and causes irreversible damage to the structure of the imprinting cavity, which seriously affects the reuse frequency of the sensor and the detection stability.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The invention belongs to the technical field of bioengineering, and discloses a specific identification primer and method for lactobacillus curvatus in human intestinal tracts and application. The identification primer comprises an upstream primer COG3825F1 and a downstream primer COG3825R1, the nucleotide sequence of the upstream primer COG3825F1 is 5 '-GTCACAACAAGACCGATTT-3', and the nucleotide sequence of the downstream primer COG3825R1 is 5 '-CTGTTTCTACAACTTGTGCT-3', and the nucleotide sequence of the upstream primer COG3825F1 and the nucleotide sequence of the downstream primer COG3825R1 are 5 '-GTCACAACAAGACCGATTT-3'. The specific COG3825 gene of the lactobacillus flexus is screened to serve as a target sequence design primer, and PCR, gel electrophoresis and qPCR verification show that the primer has extremely high specificity and sensitivity and can accurately distinguish the lactobacillus flexus from the related lactobacillus and other common intestinal bacteria under the complex micro-ecological background of the human intestinal tract; the identification method based on the primer is easy and convenient to operate and accurate in result, qualitative and quantitative detection of the lactobacillus brevis in human intestinal samples, food samples and other environment samples can be achieved, and important technical support is provided for related research, application development and micro-ecological monitoring of the lactobacillus brevis.

This invention belongs to the field of microbial technology and relates to a thermoresistant, high-esterase-producing *Changfangluode* yeast and its specific identification method. The high-esterase-producing, thermoresistant *Changfangluode* yeast (… Lodderomyces elongisporus FBKL2.9-G53, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20253046 on December 29, 2025, exhibits excellent thermostability, maintaining good growth activity and enzyme production capacity even at 45℃. Quantitative esterase analysis showed a significant thermo-enhancing effect on enzyme production, with esterase activity of 12.83 U / mL at 28℃, increasing to 14.87 U / mL at 40℃ and 20.56 U / mL at 45℃. Simulated fermentation experiments further confirmed that the strain demonstrated robust metabolic adaptability under 45℃ heat stress, with esterase activity reaching 7.44±0.35 U / mL on day 7 of fermentation, approximately 1.55 times higher than the control group at 28℃.

The present application provides a DNA barcode specific identification method of radix rehmanniae decoction pieces, and relates to the technical field of true and false identification of traditional Chinese medicine decoction pieces. The specific identification method comprises the following steps: extracting sample DNA to be detected; screening universal and specific DNA barcode genes; amplifying universal and specific DNA barcode gene fragments; amplifying the amplification product; and obtaining amplified DNA barcode sequences after sequence correction and splicing, and performing online BLAST comparison with NCBI database, so as to determine the base origin of the sample to be detected. The method provided by the present application can overcome the problem that the DNA barcode of the conventional ITS2 sequence of the decoction piece after processing is not easy to amplify, and the PCR amplification product of the present application has high quality, the molecular identification result is accurate, and the rapid identification of radix rehmanniae and radix rehmanniae preparata decoction pieces can be efficiently and high-quality solved, and the urgent need for true and false identification of radix rehmanniae decoction pieces during acceptance is solved.

The present invention relates to the technical field of chicken breed resource identification and protection, and specifically to a method for preparing and applying a chicken whole genome SNP liquid phase chip. The present invention performs whole genome resequencing on local chicken breeds and introduced breeds in Shandong Province, obtains 1142 breed-specific SNP sites, and designs 5136 SNP site low-density liquid phase chips specifically for Shandong chicken breeds in combination with local chicken SNP background sites. The chip is more targeted for Shandong chicken breed identification, has lower cost and faster speed than high-throughput sequencing detection, and has groundbreaking significance in the accurate identification of Shandong local chicken breeds, which will greatly improve the protection and utilization level of local chicken breeds in my country.

The invention discloses a crRNA for detecting porcine circovirus type 2 based on an RAA-CRISPR-Cas13d technology, a kit and application, and belongs to the technical field of molecular biology diagnos.A target sequence is efficiently amplified by adopting an isothermal amplification technology (RAA), and specific recognition and signal amplification are performed in combination with a CRISPR-Cas13d system, so that high-sensitivity and high-specificity detection is realized. Meanwhile, a simplified nucleic acid extraction method and a diversified result display technology are introduced, so that the detection process is optimized, result interpretation is simplified, and the detection efficiency is remarkably improved. By means of the improvement, the defects that an existing commercial nucleic acid extraction kit is tedious in step and complex in result interpretation are overcome, and greater possibility is provided for application of the kit in on-site instant detection.

The present invention relates to the field of bioengineering technology, and in particular to a flanking sequence of the rice GAT transformation event GATV3-470-2 and a specific identification method thereof. The right flank sequence of the flanking sequence is shown as SEQ ID NO.1; the left flank sequence of the flanking sequence is shown as SEQ ID NO.2. The present invention provides a set of specific primers for the flanking sequence of the rice transformation event GATV3-470-2, including nucleotide sequences shown as SEQ ID NO.11-12 and SEQ ID NO.9-10, which can be used to detect components derived from the rice transformation event GATV3-470-2 in biological materials. The flanking sequence of the rice transformation event GATV3-470-2 and the corresponding detection primers provided by the present invention can be used for the detection of components derived from the rice transformation event GATV3-470-2, which is of great significance in the field of biosafety assessment of transgenic rice and its derivative lines.

The invention discloses an oligonucleotide-coated fluorescence in-situ hybridization probe for specifically recognizing a tartary buckwheat Chr1-4 chromosome and application, and belongs to the field of cytological markers. The method comprises the following steps: screening a single-copy oligonucleotide sequence of Chr1-4 from a tartary buckwheat whole genome by using Chorus2 software; filtering and removing repetitive sequences to obtain a Chr1-4 chromosome probe sequence library; screening a specific single copy oligonucleotide sequence of the Chr1-4 chromosome; and adding specific primers at the two ends of each single copy oligonucleotide sequence of Chr1-4, and combining the probes into a buckwheat Chr1-4 Oligo-Painting probe mixing pool. By utilizing the constructed Oligo-Painting probe mixing pool and a fluorescence in-situ hybridization technology, accurate identification and karyotype analysis of the buckwheat chromosomes can be realized, the number, morphology, heritable variation and other structural characteristics of the chromosomes can be known, and an important technical support is provided for cytological analysis of buckwheat crossbreeding.

The invention discloses a genome editing and detecting system for recognizing a DNA target based on TDP-43 dimerization and application of the genome editing and detecting system, and belongs to the technical field of molecular biology. FokT fusion protein construction, cell transfection and functional labeling, FokT-Seq detection through high-throughput sequencing, target annotation and functional analysis and disease mutation experience verification are carried out, and the genome editing and detecting system for recognizing the DNA target based on TDP-43 dimerization is obtained. Specific recognition and functional marking on the TDP-43 dimerization dependent DNA binding site are achieved for the first time, and traditional ChIP-Seq, CUTamp, CCTP-Seq, CCTP-Seq, CCTP-Seq, CCTP-Seq; a conformational state monomer or dimer when TDP-43 is combined with DNA cannot be judged by methods such as FOkI, FOkI is only activated in a dimerization state, and only a DNA target of TDP-43 combined in a dimer form is marked, so that the structure specificity of recognition is remarkably improved, and false positive binding site interference is avoided.

This invention discloses a photoelectrochemical sensing method for detecting oxytetracycline using a differential signal strategy. The method includes the preparation of FeCu-BMO and Fe2O3@CuO nanospheres, the preparation of silver chloride cubes, and the construction of an oxytetracycline detection method. This invention prepares a bimetallic organic framework FeCu-MOF, using its oxide as a photoelectroactive material. It utilizes the specific recognition of oxytetracycline by an aptamer and the binding of the auxiliary chain on the silver chloride nanocube to the aptamer. Due to the steric hindrance effect of oxytetracycline and the quenching effect of silver chloride, a differential signal is calculated through two consecutive and correlated events, constructing a novel photoelectrochemical sensor for detecting oxytetracycline. This improves the specific binding of oxytetracycline to the sensor, achieving high-sensitivity detection of oxytetracycline. The detection limit of the differential signal strategy is 5 nmol / L, which is lower than the 22 nmol / L detection limit of the single-event photoelectrochemical sensor. Furthermore, the sensor exhibits excellent selectivity, stability, and reproducibility, making it suitable for oxytetracycline residue detection needs in various scenarios.

The invention discloses a soybean FAD2 gene mutation site, a specific identification primer and a method for increasing the content of oleic acid in soybean, the soybean provided for the first time after the specific mutation sites of FAD2-1A and FAD2-1B genes are mutated can significantly increase the relative content of oleic acid in soybean seeds, the relative content reaches 70% or above, and meanwhile the content of linoleic acid is reduced to 20% or below. When the specific primer is used for detecting the corresponding mutant gene, a specific band exists, the efficiency reaches 100%, and the accuracy is high. The mutation does not influence the normal growth and development and other agronomic characters of the soybeans while obviously improving the oleic acid content, ensures the yield and other comprehensive properties of the soybeans, and is beneficial to large-scale popularization and planting. The mutation site and related technologies have wide application prospects in soybean breeding and soybean product production, can be used for cultivating different types of high oleic acid soybean varieties, meet various market requirements, and also can be used for improving the quality of various soybean products.

The present invention discloses a primer set for specific identification of molecular markers of transgenic glyphosate-resistant cotton ZD131 and its application. The primer set comprises two pairs of primers, namely primer pair ZD131LB and primer pair ZD131RB; the sequences of primer pair ZD131LB are SEQ ID NO.1 and SEQ ID NO.2; the sequences of primer pair ZD131RB are SEQ ID NO.3 and SEQ ID NO.4. The present invention designs and constructs primer pairs ZD131LB and ZD131RB, which can specifically identify and identify the insertion site of ZD131 exogenous gene.

The present invention discloses a method for detecting Alternaria using a thermometer based on CRISPR / Cas12a. The present invention utilizes a dual signal amplification strategy of high specific surface area of ​​AuNPs and efficient shearing ability of LbCas12a to achieve effective amplification of detection signals, and has very high sensitivity to the detection of Alternaria; at the same time, the present invention utilizes CrRNA and target DNA for specific identification, and has very good specificity for the detection of Alternaria, and can achieve rapid, accurate and sensitive detection of Alternaria in the sample to be tested; in the present invention, there is no need to use large-scale detection instruments and toxic reagents. In the detection method of the present invention, portable on-site detection of Alternaria can be achieved using only a thermometer.

The application belongs to the field of chemical detection, and relates to a Schiff base copper ion colorimetric fluorescent probe molecule and a preparation method and application thereof. The structural formula of the Schiff base copper ion colorimetric fluorescent probe molecule is: the preparation method has simple synthesis steps, and only needs to pass through one-step reaction, and has high yield. The fluorescent probe provided by the application exhibits high efficient and specific identification performance on copper ions in a PBS buffer solution system, has the advantages of good selectivity, strong anti-interference ability, fast response speed (response can be completed within 30 seconds), low detection limit (18 nM), and a wide pH value application range; colorimetric experiment results show that the probe can realize naked-eye detection on copper ions, and has wide application prospects in the fields of public safety, environmental monitoring and the like.

The invention discloses a primer group, a kit and a method for identifying deer species based on IGF1 (Insulin-like Growth Factor 1), IGF2 (Insulin-like Growth Factor 2) and RAF1 genes. The primer group comprises three pairs of specific primers aiming at IGF1, IGF2 and RAF1 genes, and the sequences of the three pairs of specific primers are respectively SEQ ID NO: 12 and 13, SEQ ID NO: 14 and 15, and SEQ ID NO: 16 and 17. In specific identification, DNA of a sample is extracted, PCR amplification is carried out by using the primer group, and then the fragment length difference of an amplification product is analyzed through agarose gel electrophoresis. By comparing specific length combinations of IGF1, IGF2 and RAF1 gene amplified fragments, the four types of cervidae animals can be accurately distinguished. According to the application, the identification system for the cervidae species based on the IGF1, IGF2 and RAF1 genes is established for the first time, and the identification system has the advantages of rapidness, accuracy, low cost and no need of sequencing, and is suitable for species source identification and market supervision of related products.

This application relates to the field of quality testing technology and provides a method for quality testing of GABA extract in peanut sprouts. It utilizes a molecularly imprinted sensor for specific identification of GABA, combined with a pre-set peanut sprout interference database and partial least squares regression to calculate impurity contribution. This method can specifically eliminate interference from complex matrix impurities in peanut sprout extracts, solving the problem of insufficient specificity in traditional methods for complex samples. The molecularly imprinted sensor is connected in series with a quartz crystal microbalance via a microfluidic channel. Capacitance changes trigger resonant frequency detection, creating a coherent process from identification to quality analysis and improving detection efficiency. By inputting corrected frequency offset, phase angle, and temperature into a recurrent neural network, the method integrates multi-dimensional detection information to achieve accurate output of GABA concentration. Compared to single-parameter detection methods, this method more comprehensively reflects the true content of GABA in the extract, providing a reliable basis for quality judgment.

The present invention discloses a method for interpreting the results of gynecological dry chemistry tests. Reaction colors for different test results are selected in a two-dimensional color space; a two-dimensional partition map interpretation model is constructed; the coordinate values of the RGB values of the reaction colors for different test results in the two-dimensional color space are substituted into the two-dimensional partition map interpretation model, and the test results are obtained according to the interpretation area where the coordinate values are located. The advantages of the present invention are that based on the principle most favorable for the segmentation of sample experimental identification points, the reaction colors used to represent different test results are autonomously selected, which can improve the accuracy of the interpretation area division and the scalability of the interpretation results of the interpretation model. Based on the principle that the coincidence rate of the consistency between the manual interpretation results and the machine-readable results meets the requirements and the number of specific identification points of the test results in adjacent interpretation areas is the same, the interpretation experience of professional qualification interpreters is quantified into the interpretation method to achieve the optimal division of the interpretation area, overcoming the problems existing in the existing manual interpretation.

The application discloses a multiplex PCR specific identification primer for a pheasant meat source component, and the nucleotide sequence of a pheasant specific primer is 5'-GTAAAGTAGGACCATGCCTCT-3'. The application further discloses a kit containing the multiplex PCR specific identification primer, and a multiplex PCR identification method for the pheasant meat source component. The pheasant specific primer provided by the application can perform PCR amplification on 12S rRNA, the amplification effect is stable, a 151bp specific band can be generated, and the pheasant meat source can be effectively identified.

The present application belongs to the field of biotechnology, and particularly relates to a biomarker for differential diagnosis of recent and long-term tuberculosis infection. The present application determines the differential proteins in blood of Mycobacterium tuberculosis recent infection and long-term infection through proteomics, and provides a diagnostic biomarker for differential diagnosis of Mycobacterium tuberculosis recent infection and long-term infection. The biomarker provided by the present application has good diagnostic efficiency whether it is used for single diagnosis or combined diagnosis, and the AUC value is greater than or close to 0.8. The biomarker can be used for more rapid, efficient, sensitive and specific identification of Mycobacterium tuberculosis recent infection, and provides a diagnostic basis for early intervention of tuberculosis.

This invention discloses a high-throughput quality control method for Qingfei Paidu Granules. Based on liquid chromatography-mass spectrometry, this method establishes a comprehensive quality control method for Qingfei Paidu Granules. This method enables specific identification of the 19 ingredients in the Qingfei Paidu Granules, limit testing of the toxic component aristolochic acid I, fingerprint analysis, and content determination of four indicator components in a single analysis, significantly improving detection efficiency and reducing testing costs. This method enables rapid, comprehensive, and accurate quality assessment of Qingfei Paidu Granules, providing technical support for the development of new Qingfei Paidu Granules.