49 results about "Panax stipuleanatus" patented technology

The invention relates to an SNP (Single Nucleotide Polymorphism) marker for rapidly identifying panax stipuleanatus and counterfeit species thereof. The nucleotide sequence of the SNP marker is shown as SEQ ID No.1; an 88th site from a 5 end of the sequence is A and/or a 117th site is C. The invention further relates to a kit for rapidly identifying the panax stipuleanatus and the counterfeit species thereof, and the kit comprises a primer which is used for amplifying to obtain the sequence shown as SEQ ID No.1. The invention further relates to a method for rapidly identifying the panax stipuleanatus and the counterfeit species thereof; the method comprises the following steps: taking a genome DNA (Deoxyribonucleic Acid) of a sample to be identified as a template and amplifying the sequence shown as SEQ ID No.1; detecting an 88th site basic group and a 117th site basic group from the 5 end in an obtained amplified fragment, so as to judge that the sample to be identified is the panax stipuleanatus or the counterfeit species thereof. By adopting the method provided by the invention, the panax stipuleanatus and the counterfeit species with similar morphological characteristics can be accurately distinguished; the method has wide applicability and can be used for detecting any sample of the panax stipuleanatus capable of being used for extracting the DNAs, and rapid and accurate identification of the panax stipuleanatus can be realized.

The invention discloses a primer combination for simultaneously identifying Chinese herb ginseng, American ginseng and radix notoginseng and application of the primer combination. The primer combination consists of six single-chain DNA molecules as shown in sequences 1 to 6. The invention also relates to application of the primer combination in identifying the ginseng, the American ginseng and the radix notoginseng. The detection method provided by the invention has a detection limit of 1 percent for the mutual doping of any two of the ginseng, the American ginseng and the radix notoginseng, and can meet the requirement on the production and inspection of Chinese herbs. The primer combination can be used for identifying roots, stems, leaves, flowers, fruits and processed products of the ginseng, the American ginseng and the radix notoginseng.

The invention discloses a Dendrobium officinale Panax quinquefolius buccal tablet. Per 1000g buccal tablet is prepared from the following raw materials by weight: 300-400g of fresh Dendrobium Panax strips, 300-400g of fresh Panax quinquefolius roots, and 200-400g of sugar. Compared with traditional buccal tablets, during preparation of the Dendrobium officinale Panax quinquefolius buccal tablet, the Dendrobium officinale and the Panax quinquefolius are milled into nanopowder, so that release of active components contained in the two substances can be easy. Meanwhile, by combining the characteristic that glucose is very convenient to be absorbed by human bodies, the buccal tablet can improve the mental condition of human bodies in a short time. Long-term taking of the product can effectively alleviate physical weakness, anenergia, hypomnesis and other symptoms.

The invention relates to a teabag fruit mate tea, which takes mate stem leaves as main ingredients, and is added with fruits and less amount of Chinese herbal medicines. The ingredients are mixed according to the following weight ratio: 55-70 percent of mate stem leaves, 15-35 percent of dried fruits, 2-5 percent of glycyrrhiza, 1-5 percent of wolfberry fruit, 2-5 percent of dried orange peel, and 1-3 percent of American ginseng. The processed mate stem leaves, the processed dried fruits, the processed glycyrrhiza, the processed wolfberry fruit, the processed dried orange peel and the processed American ginseng are packaged into a permeable cotton bag; and the weight of each bag is 3-5g. The mate tea has the healthy significances in adjusting mental state, balancing mental functions, purifying in-vivo environments, promoting blood quality, promoting metabolism and enhancing disease-resistant constitution. Strawberries, apples, lemons, oranges and other different fruits are added in the mate tea, so that the nutrition of vitamin in the fruits can be obtained, the problem of bad taste can be solved, and taste needs of different customers are met.

The invention provides polygonatum rhizome health-care milk tea and its preparation method. The health-care milk tea comprises the following components: by weight, 2-15% of black tea powder, 5-20% of glucose, 3-10% of Chinese herb extracts, 20-35% of milk powder, 15-30% of white sugar or xylitol and 0.1-1.0% of a thickening agent. The Chinese herb extracts comprise the following ingredients: by weight, 1-10 parts of polygonatum rhizome, 1-10 parts of wolfberry and 1-10 parts of American ginseng. The preparation method comprises the following steps: carrying out normal-pressure high-temperature extraction on the carefully-chosen polygonatum rhizome, wolfberry and American ginseng so as to obtain Chinese herb crude extracts; placing the Chinese herb crude extracts into a jacketed pan for evaporative concentration, uniformly mixing the concentrated Chinese herb crude extracts with black tea powder, glucose, the Chinese herb extracts, milk powder, white sugar or xylitol and the thickening agent, and drying the mixture in a constant-temperature drying box of 65-75 DEG C for 2-2.5 hours; and crushing the dried mixture in a pulverizer, sieving the crushed mixture through a 60-mesh sieve, and packaging the powdery mixture to obtain the finished product. According to the invention, taste of milk tea can be retained, and the purpose of cultivation and health preservation also can be achieved.

Belonging to the technical field of traditional Chinese medicine, the invention relates to identification methods of Chinese herbal medicines, in particular to a specific molecular marker for panax ginseng and panax quinquefolius and an identification method thereof. The invention provides a DNA molecular marker for specific identification of panax ginseng and panax quinquefolius. The DNA molecular marker is located on the (SSR) loci of DNA sequences Seq.No.1-5 respectively. 5 pairs of primers are designed according to the sequences to perform PCR (polymerase chain reaction) amplification on the DNA templates of panax ginseng and panax quinquefolius. PCR products for specific identification of panax ginseng and panax quinquefolius can be amplified respectively by each pair of the primers, the products with different lengths are detected by polyacrylamide gel electrophoresis and agarose gel electrophoresis, so that panax ginseng and panax quinquefolius can be rapidly identified. The method needs a small amount of samples, is not restricted by product shapes, and has low requirement for sample's DNA length. The PCR is stable and has good repeatability.

The invention relates to a preparation method and application of anti-inflammatory effective parts of Panax stipuleanatus, and belongs to the technical field of biological medicine. The preparation method comprises the following steps: after smashing Panax stipuleanatus, carrying out digestion on methyl alcohol, and carrying out vacuum concentration on a leach liquor to obtain a methyl alcohol extract; dispersing the obtained methyl alcohol extract with water, and extracting with petroleum ether to obtain an extraction raffinate water solution for removing fat-soluble impurities; extracting the obtained extraction raffinate water solution with saturated n-butyl alcohol, and extracting an organic solution to obtain an n-butyl alcohol extract through vacuum concentration; and separating theobtained n-butyl alcohol extract through silica gel column chromatography, carrying out elution by using a dichloromethane/methyl alcohol solvent system, and concentrating and drying an eluant to obtain the anti-inflammatory effective parts of Panax stipuleanatus. The effective parts of Panax stipuleanatus can be effectively prepared on large scale, and the application of Panax stipuleanatus in the preparation of anti-inflammatory drugs is realized.

The invention belongs to the technical field of marine organisms, and particularly relates to American ginseng and trepang capsules and a preparation method thereof. The American ginseng and trepang capsules comprise the following components in percentage by mass: 30%-35% of American ginseng, 50%-60% of dried trepang and 10%-15% of beta-cyclodextrin. According to the American ginseng and trepang capsules and the preparation method thereof disclosed by the invention, a high dosage of the trepang is used, the largest ratio of the trepang to the American ginseng is 2 to 1, the American ginseng is cool in property, but the trepang is hot in property, in the appropriate ratio of the American ginseng to the trepang, the trepang can sufficiently resist the cool property of the American ginseng through the autologous hot property of the trepang, and the side effects of fear of cold, body temperature drop, inappetency, abdominal pain and diarrhoea after the American ginseng is eaten can be resisted.

The invention discloses a preparation method of panax quinquefolius ultrafine blunt powder oral decoction tablets. The preparation method comprises the following steps: 1, dedusting and cleaning a panax quinquefolius raw material; 2, drying the panax quinquefolius raw material; 3, putting the panax quinquefolius raw material well treated in the step 2 into liquid nitrogen, and crushing at minus 196 DEG C; 4, putting drying the crushed panax quinquefolius raw material in a low pressure place for drying, and controlling the water content to be less than 20%; 5, performing cobalt-60 irradiation on the ultrafine powder formed in the step 4, and tabletting to obtain the oral decoction tablets. By adopting the preparation method with novel steps, the oral decoction tablets which are convenient to use are finally prepared, the biological availability of the product is improved, the biological availability of active ingredients of the panax quinquefolius can be effectively improved, the pure powder tablets can ensure the product quality, the appearance properties and hardness of the product meet quality standard requirements of tablets, and the dissolution rate is increased.

The invention relates to some panax notoginseng and panax stipuleanatus disease-course correlated function genes, and the genes are obtained from panax notoginseng and panax stipuleanatus by firstly utilizing RT-PCR, and the detailed sequence is shown as a sequence table 1. A complex mechanism is developed for resisting harmful biont invasion in environment by plants in the long-term evolution process, and when pathogen invades, whether disease-resistant correlated genes can be effectively induced and the expression level difference are basic reasons leading a host plant to finally show a disease-resistant or disease-infection phenomenon. Therefore, cloning of the genes is beneficial for revealing the disease-resistant molecular mechanism of notoginseng and other medical plants, and has important theory guidance meaning on culture of future notoginseng disease-resistant new species.

The invention discloses a strain of Penicillium fungus M1 and application thereof to increase of saponins yield in a fermentation process of ginseng or American ginseng, and belongs to the technical field of biotechnology. According to the invention, functional fungus is used for fermentation cultivation of ginseng or American ginseng; the content of saponins before and after the fermentation are analyzed and compared; and finally a bacterial strain capable of significantly increasing saponins yield in the fermentation process of the two herbs is obtained. The strain is identified as Penicillium fungus (Penicillium sp.), and named after Penicillium fungus M1, and has a preservation number of CGMCC NO.6359. The strain can be used in fermentation production of ginseng or American ginseng; and the content of saponins in fermentation products fermented by the strain is greatly improved. Therefore, according to the present invention, Penicillium fungus has broad application prospects in increasing saponins yield in the fermentation process of ginseng or American ginseng.

The invention discloses a radix et rhizoma ginseng, radix panacis quinquefolii and radix notoginseng solid fermentation processing method. After radix et rhizoma ginseng, radix panacis quinquefolii and radix notoginseng are fermented and biologically converted by schizophyllum commune, ginsenoside in the three medicinal materials can be converted into rare ginsenoside, lignocelllulose of the threemedicinal materials is also decomposed and utilized by the schizophyllum commune, a lot of biological active matter of schizophyllan and the like can be generated, and effects of the radix et rhizomaginseng, the radix panacis quinquefolii and the radix notoginseng are remarkably improved; thus, the shortcomings that traditional processed products of the radix et rhizoma ginseng, the radix panacis quinquefolii and the radix notoginseng have a low rare ginsenoside content, a low bioavailability and the like are overcome.

The invention discloses a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for quickly identifying panax notoginseng and analogue panax stipuleanatus. According to the method, a primer adopted by PCR amplification in the method consists of an ITS (internal transcribed spacer) forward primer and an ITS reverse primer, the base sequence of the forward primer is as shown by SEQ ID NO:1, and the base sequence of the reverse primer is as shown by SEQ ID NO:1; and after a PCR amplification product is purified, a restriction enzyme MluC I and a restriction enzyme Nla IV are respectively used for carrying out enzyme digestion, and in an MluC I enzyme-digestion map and an Nla IV map, the enzyme-digestion maps of the panax notoginseng and the panax stipuleanatus are completely different, so that whether a sample is the panax notoginseng and whether the analogue panax stipuleanatus is mixed into the sample can be identified. By adopting the method, restriction enzyme enzyme-digestion maps of the panax notoginseng and the panax stipuleanatus are successfully established for the first time, the panax notoginseng and the panax stipuleanatus can be accurately and quickly identified, and the problem that sensory the authenticity of a panax notoginseng raw material cannot be easily identified through evaluation is solved.

The invention discloses an American ginseng flavored dendrobium officinale beverage and a preparation process thereof. The American ginseng flavored dendrobium officinale beverage is prepared from the following components in parts by weight: 0.05 to 0.20 part of stevia honey leaf, 0.03 to 0.08 part of radix ginseng extract, 0.03 to 0.15 part of guarana extract, 0.03 to 0.08 part of dendrobium officinale extract, 4 to 8 parts of concentrated apple juice, 1.0 to 1.5 parts of citric acid, 1.0 to 1.5 parts of malic acid, 0.2 to 0.6 part of sodium citrate, 0.1 to 0.3 part of edible essence and the balance of pure water, wherein the total amount is 1000 parts. By scientifically proportioning the dendrobium officinale extract, the radix ginseng extract, guarana, the concentrated apple juice, the citric acid, the malic acid, the sodium citrate and the edible essence, the obtained beverage has a unique American ginseng flavor and has the effects of nourishing yin and moistening lung, nourishing stomach and regenerating body fluid, clearing away heat and detoxifying and regulating yin and yang so that an organism reaches yin-yang balance.

The invention belongs to the technical field of food processing and particularly relates to a preparation method of a radix panacis quinquefolii and red jujube composited health beverage. The preparation method of the radix panacis quinquefolii and red jujube composited health beverage comprises steps as follows: 1), preparation of a radix panacis quinquefolii extract solution; 2), preparation ofred jujube juice; 3), preparation of flos lonicerae juice; 4), blending; 5), filtering and filling; 6), sterilization. The beverage prepared with the method has the healthcare effect of enhancing immunity, ginsenoside as a functional component of radix panacis quinquefolii extract has bitter and astringent taste, the red jujube juice can correct the bitter and astringent taste of the ginsenoside,and the taste of the beverage is enabled to be richer by the flos lonicerae juice; the red jujube and flos lonicerae both can perform synergistic interaction in the aspect of immunoregulation.

The present invention belongs to the field of health-care products and particularly relates to a health-care food containing green plums and capable of assisting in reducing blood lipid and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 18-35 parts of green plums, 10-22 parts of rhizoma alismatis, 6-18 parts of radix salviae miltiorrhizae, 8-12 parts of saffron, 10-18 parts of trogopterus dungs, 2-8 parts of pollen typhae, 12-20 parts of radix polygoni multiflori, 4-10 parts of panax stipuleanatus roots, 4-10 parts of radix bupleuri, 2-8 parts of haws, 4-10 parts of lucid ganoderma and 12-24 parts of licorice. The provided health-care food composition is reasonable and scientific in compatibility, and each of the components has synergistic effects, so that the health-care food composition has efficacies of activating blood and stimulating menstrual flow, promoting qi and dispelling stasis, removing blood stasis and treating stranguria, harmonizing the exterior and interior, clearing away heat and toxic substances, etc. An animal experiment shows that the health-care food has obvious auxiliary effects of lowering blood lipid, and is less in toxic or side effects and high in safety.

The invention discloses angelica sinensis honey liquor which is prepared through conventional technologies from following components, by weight, 40 parts of angelica sinensis, 60 parts of honey, 15 parts of American ginseng, 10 parts of fructus lycii, 15 parts of poria cocos, 10 parts of semen cassiae, 3 parts of bezoar from monkey and 1000 parts of liquor. The components, except the liquor, are placed in a container with addition of the liquor. The container is sealed and the components are soaked in the liquor for 15 days. Filtration is carried out to remove residues to obtain the angelica sinensis honey liquor. The angelica sinensis honey liquor has effects of nourishing blood and retaining youthful looks and is mainly used for treating skin pigmentation, facial acne, dryness and sparsity in hair and the like. The angelica sinensis honey liquor has effects of nourishing and fortifying body, caring health and beautifying face, and delaying senescence. The angelica sinensis honey liquor is taken orally with a dosage being 15ml by two times per day.

The invention discloses a medium solution for panax stipuleanatus artificial pollination. The medium solution is prepared from the following ingredients in percentage by weight: 0.005 to 0.02 percent of boracic acid, 0.5 to 1.5 percent of saccharose, 0.1 to 0.2 percent of monopotassium phosphate, 0.005 to 0.02 percent of calcium chloride and the balance water. The medium solution provided by the invention can provide nutrition elements for pollination and pollen implantation; the fruiting rate after the pollination can be greatly improved; after the medium solution formed by mixing the boracic acid, saccharose, monopotassium phosphate and calcium chloride is applied onto the pollen during the pollination, the pollen germination is promoted; the fertilization probability is improved.

The invention discloses herba rhodiolae health-care capsules and a preparation method thereof. The herba rhodiolae health-care capsules contain herba rhodiolae, American ginseng and hippophae rhamnoides fruits. The preparation method of the herba rhodiolae health-care capsules comprises the following steps of thoroughly cleaning raw medicinal materials of the herba rhodiolae, the hippophae rhamnoides fruits and the American ginseng, and performing airing; drying the treated American ginseng, crushing the dried American ginseng, and screening the crushed American ginseng with a 100-mesh sieve;performing reflux extraction on the treated herba rhodiolae and the treated hippophae rhamnoides fruits with 70% ethanol, combining extraction solutions, performing filtration, performing drying underreduced pressure, then performing crushing, and performing screening with an 80-mesh sieve; and uniformly mixing obtained powder, making the mixed powder into soft materials with ethanol of which thepercentage by mass is 80%, performing granulation with a 20-mesh sieve, performing drying at 55-60 DEG C, performing granule trimming with a 16-mesh sieve, performing loading into No.0 capsules to obtain capsules, and performing packaging so as to obtain the herba rhodiolae capsules.

The invention provides poria cocos health-care wine and a preparation method thereof. The poria cocos health-care wine is prepared from the following raw materials in parts by mass: 500 to 600 parts of original-liquor Baijiu, 45 to 55 parts of poria cocos, 20 to 25 parts of long-noded pit viper/zaocys dhumnade, 15 to 20 parts of rhizoma imperatae, 10 to 15 parts of radix panacis quinquefolii, 10 to 15 parts of cervi cornu pantotrichum, 5 to 10 parts of rhizoma atractylodis macrocephalae, 5 to 10 parts of rhizoma pinelliae, 5 to 10 parts of pericarpium citri reticulatae, 5 to 10 parts of fructus lycii and 2 to 3 parts of cortex cinnamomi. The preparation method comprises the following steps: carrying out stir-frying treatment on all the raw materials and then steaming over water so as to more conveniently separate out all traditional Chinese medicine effective components in a wine brewing process; then dissolving the traditional Chinese medicine effective components into a wine body. The poria cocos health-care wine has the advantages of rich wine aroma, remarkable pharmaceutical effect and capabilities of effectively resisting ageing, calming the heart and tranquilizing the mind and enhancing the organism immunity.

The invention provides a preparation method of radix panacis quinquefolii slices. The method comprises the following steps that raw-skin radix panacis quinquefolii sticks are selected as a raw material; the raw-skin radix panacis quinquefolii is infiltrated and wetted; the wetted raw-skin radix panacis quinquefolii is put into a radix panacis quinquefolii steaming tray for low-temperature softening, wherein the temperature is 70-80 DEG C, and the softening time is 0.8-1 hour; the softened radix panacis quinquefolii sticks are taken out and sliced by an automatic slicer; a low-temperature far-infrared negative-pressure drying method is adopted for drying, wherein the drying temperature is 30 DEG C, the drying time is 24-36 hours, and the water content is reduced to 13% or below; the radix panacis quinquefolii slices are divided into superior products, first-class products and qualified products according to the appearance quality and packaged separately. The method can avoid the damageof high-temperature heating to effective constituents of the radix panacis quinquefolii, thereby obtaining the radix panacis quinquefolii slices with well kept nutritive value.