Specific molecular marker for panax ginseng and panax quinquefolius and identification method thereof

A technology of molecular markers and American ginseng, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc. Effects with low length requirements

Inactive Publication Date: 2014-07-16
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prior art discloses that the medicinal properties of ginseng and American ginseng are quite different, but they are similar in shape, especially the domestically cultivated

Method used

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  • Specific molecular marker for panax ginseng and panax quinquefolius and identification method thereof
  • Specific molecular marker for panax ginseng and panax quinquefolius and identification method thereof
  • Specific molecular marker for panax ginseng and panax quinquefolius and identification method thereof

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Embodiment 1

[0034] Five common ginseng farm varieties, Damaya, Ermaya, Longneck, Yuanlu and Bamboo-joined reed, and 51 samples of Yishan ginseng, Linxia ginseng, Shizhu ginseng and Korean ginseng were collected from different origins and markets. 16 samples of American ginseng collected from China, Canada and Liaoning, China (the materials are shown in Table 2);

[0035] The total DNA of the sample was extracted by the improved high-salt SDS method, and high-quality DNA was obtained from less material;

[0036] PCR amplification, PCR reaction system is as follows: total volume 20 μL, containing about 20ng genomic DNA, forward primer and reverse primer each 0.25 μmol / L, 1× reaction buffer, 1.0mmol / L MgCl2, 0.1mmol / L dNTP, For 1U Taq enzyme, use DEPC-treated water to make up the volume to 20 μL, and use deionized water instead of template DNA as a negative control;

[0037] The PCR amplification procedure is as follows: PCR reaction in Applied Biosystem TM 2720 ​​Thermal Cycler amplificat...

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Abstract

Belonging to the technical field of traditional Chinese medicine, the invention relates to identification methods of Chinese herbal medicines, in particular to a specific molecular marker for panax ginseng and panax quinquefolius and an identification method thereof. The invention provides a DNA molecular marker for specific identification of panax ginseng and panax quinquefolius. The DNA molecular marker is located on the (SSR) loci of DNA sequences Seq.No.1-5 respectively. 5 pairs of primers are designed according to the sequences to perform PCR (polymerase chain reaction) amplification on the DNA templates of panax ginseng and panax quinquefolius. PCR products for specific identification of panax ginseng and panax quinquefolius can be amplified respectively by each pair of the primers, the products with different lengths are detected by polyacrylamide gel electrophoresis and agarose gel electrophoresis, so that panax ginseng and panax quinquefolius can be rapidly identified. The method needs a small amount of samples, is not restricted by product shapes, and has low requirement for sample's DNA length. The PCR is stable and has good repeatability.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine and relates to a method for identifying Chinese medicinal materials. Specifically, it relates to a specific molecular marker of ginseng and American ginseng and its identification method. Background technique [0002] Ginseng (Panax ginseng C.A. Meyer) and American ginseng (Panax quinquefolius L.) are currently the most widely used important Chinese herbal medicines in my country, belonging to the perennial herb Araliaceae (Araliaceae) ginseng. Studies have shown that ginseng is distributed in Northeast my country, the Far East of Russia, and North Korea; American ginseng is native to low mountain areas in North America. The prior art discloses that ginseng and American ginseng are quite different in medicinal properties, but they are similar in shape, especially the ginseng cultivated in China is difficult to accurately identify according to the morphological characteristics...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张文驹陈子易程舟陈家宽
Owner FUDAN UNIV
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