Cloning of panax notoginseng and panax stipuleanatus disease-course correlated function genes

A technology related to functions and genes, which is applied in the field of cloning Panax notoginseng and Pingbian Panax notoginseng disease course-related functional genes, can solve the problems that there are no related reports in the research of other medicinal plants

Inactive Publication Date: 2015-01-14
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Problems solved by technology

The present invention uses the EST data of Panax notoginseng and Panax notoginseng, through sequence splicing, gene function annotation, and KEGG mapping, and finally successfully clones some disease-course-related functional genes in Panax notoginseng and Pingbian notoginseng. There is no relevant report in the research of other medicinal plants

Method used

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  • Cloning of panax notoginseng and panax stipuleanatus disease-course correlated function genes
  • Cloning of panax notoginseng and panax stipuleanatus disease-course correlated function genes
  • Cloning of panax notoginseng and panax stipuleanatus disease-course correlated function genes

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Embodiment Construction

[0017] 1. Extraction and detection of total RNA of Panax notoginseng and Panax notoginseng

[0018] Take Yunnan Wenshan notoginseng (panax notoginseng) and Pingbian notoginseng (panax stipuleanatus) 1g each, quickly grind them into powder with liquid nitrogen in a mortar, take 0.1g of the ground sample and put it in a 1.5ml centrifuge tube without RNase Add 1ml of Trizol to the pulverized sample, gently invert and mix, and place at room temperature for 5 minutes; centrifuge at 12,000 rpm for 5 minutes at 4°C; take the supernatant into a new EP tube, add 200 μL of chloroform, shake vigorously for about 15 seconds, Place at room temperature for 3 minutes; centrifuge at 12,000 rpm for 15 minutes at 4°C; take the supernatant into a new EP tube, making sure not to suck tissue fragments. Add 500 μL of isopropanol and mix gently up and down, and place at room temperature for 10 minutes; centrifuge at 12,000 rpm for 10 minutes at 4°C; at this time, there will be a white precipitate at...

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Abstract

The invention relates to some panax notoginseng and panax stipuleanatus disease-course correlated function genes, and the genes are obtained from panax notoginseng and panax stipuleanatus by firstly utilizing RT-PCR, and the detailed sequence is shown as a sequence table 1. A complex mechanism is developed for resisting harmful biont invasion in environment by plants in the long-term evolution process, and when pathogen invades, whether disease-resistant correlated genes can be effectively induced and the expression level difference are basic reasons leading a host plant to finally show a disease-resistant or disease-infection phenomenon. Therefore, cloning of the genes is beneficial for revealing the disease-resistant molecular mechanism of notoginseng and other medical plants, and has important theory guidance meaning on culture of future notoginseng disease-resistant new species.

Description

technical field [0001] The present invention relates to a technique for cloning Panax notoginseng and Pingbian Panax notoginseng disease course-related functional genes by using RT-PCR technology. Background technique [0002] Plant disease is a major limiting factor in agricultural production, and medicinal plants also face the same problem in their cultivation. After plants are infected by diseases, they often show a series of problems such as serious continuous cropping obstacles, sharply reduced yields, reduced medicinal active ingredients, and poor quality. [0003] Sanqi [Panax notoginseng (Burk) F.H.Chen] is a plant of the genus Ginseng of Araliaceae, also known as "Southern God Grass", "Gold Doesn't Change", etc. It is one of the important precious Chinese medicinal materials in my country. Panax notoginseng belongs to the root tuber used as medicine, which has obvious effects of stopping bleeding, promoting blood circulation and removing stasis, reducing swelling a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 黄璐琦申业崔秀明李瑞博刘玉忠
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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