Establishment method of animal model for acute Stanford A-type aortic dissection (AD) accompanied with MODS (multiple organ dysfunction syndrome)

A technology for aortic dissection and animal model, applied in animal husbandry and other directions, can solve the problems of limited tear length of dissection, low success rate of dissection, and no related records of accompanying MODS.

CN102334465AInactive Publication Date: 2012-02-01WEST CHINA HOSPITAL SICHUAN UNIV +2
0 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2012-02-01
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides an establishment method of an animal model for acute Stanford A-type aortic dissection (AD) accompanied with MODS (multiple organ dysfunction syndrome), a use of emodin for preparing a medicine for treating acute Stanford A-type AD accompanied with MODS as well as a viscera protective agent for acute Stanford A-type AD accompanied with MODS. In the invention, an AD surgery method is improved and meanwhile manually controlled hypertension is formed by continuously pumping adrenaline into veins so as to promote expansion of the AD stripping range and accelerate disease progression, thus acute Stanford A-type AD accompanied with MODS can be rapidly formed so as to successfully make the model. The animal model for acute Stanford A-type AD accompanied with MODS creates conditions for further deep research in application foundation and intervention treatment.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The invention relates to a method for establishing an animal model of acute Stanford A type aortic dissection with MODS. Background technique

[0002] Acute aortic dissection (AD) refers to the occurrence of a tear in the middle layer of the aorta, and blood enters the false cavity through the tear in the intima-media. It is a critical emergency in cardiovascular surgery, with rapid progress and high mortality. The early mortality rate is about 1% per hour, and the mortality rate can be as high as 50% within 48 hours. There are many types of aortic dissection. In terms of guiding clinical treatment, the Stanford type is the most commonly used and most valuable. Stanford type A aortic dissection refers to the lesion involving the ascending aorta with or without involving the descending aorta, and the Stanford type B aortic dissection refers to the lesion involving only the descending aorta. After the occurrence of aortic dissection, arterial blood flow con...

Examples

Embodiment 1

[0054] Example 1 Method for establishing an animal model of acute Stanford type A aortic dissection with MODS of the present invention

[0055] 1 material

[0056] 1.1 Experimental animals

[0057] There were 18 healthy adult Beagle dogs, both male and female, weighing 8-12kg, with an average of 8.95±0.72kg. Before the operation, each animal was kept in a separate cage at a room temperature of 23~25℃, with free food and water, 12 hours of light / darkness. The dogs were provided by the West China Clinical School of Sichuan University / West China Hospital Traditional Chinese Medicine Safety Evaluation Center, and were bred by the animal room of the West China Clinical School of Sichuan University / West China Hospital Experimental Animal Center according to the requirements of experimental animals.

[0058] 1.2 Main experimental reagents and drugs

[0059] 1) Heparin Sodium (Shanghai Biochemical Pharmaceutical Factory)

[0060] 2) Penicillin Sodium (North China Pharmaceutical)

[0061] 3) Adr...

Embodiment 2

[0197] Example 2 The use of classic drugs for treating acute Stanford type A aortic dissection with MODS to verify the animal model of the present invention and initially establish a related drug screening system

[0198] ①. Establish an animal model of acute Stanford A aortic dissection with MODS according to the method described in Example 1.

[0199] ② Apply the classic drugs and candidate drugs for the treatment of acute Stanford A aortic dissection with MODS to animal models;

[0200] ③Compared with classic drugs for the treatment of acute Stanford type A aortic dissection with MODS, observe the influence of candidate drugs on acute Stanford type A aortic dissection with MODS, as well as the improvement of symptoms and indicators, and score and evaluate potential treatment The drugs for acute Stanford A-type aortic dissection with MODS also verify the feasibility of the animal model of the present invention for drug screening.

[0201] After the evaluation of step c, the classic ...

experiment example 3

[0203] Experimental example 3 The protective effect of rhubarb on organs with acute Stanford A aortic dissection with MODS

[0204] 1 material

[0205] 1.1 Experimental animals

[0206] There are 12 healthy adult Beagle dogs, both male and female, weighing 8-12kg, with an average of 8.88±0.80kg. Before the operation, each animal was kept in a separate cage at a room temperature of 23~25℃, with free food and water, 12 hours of light / darkness. The dogs were provided by the West China Clinical School of Sichuan University / West China Hospital Traditional Chinese Medicine Safety Evaluation Center, and were bred by the animal room of the West China Clinical School of Sichuan University / West China Hospital Experimental Animal Center according to the requirements of experimental animals.

[0207] 1.2 Main experimental reagents and drugs

[0208] 1) Heparin Sodium (Shanghai Biochemical Pharmaceutical Factory)

[0209] 2) Penicillin Sodium (North China Pharmaceutical)

[0210] 3) Adrenaline (Shan...