2 results about "North china" patented technology

This invention discloses a primer combination for water-saving and quality screening of winter wheat and its application, relating to the field of molecular biology. The primer combination includes primer pair P1 and primer pair P2. This primer combination is used for molecular marker screening of water-saving and drought-resistant traits in winter wheat and can be combined with SDS-PAGE electrophoresis to detect grain protein subunit composition for screening quality traits in winter wheat. This invention designs specific primer combinations, constructs a molecular marker screening system, and innovatively combines it with the "pre-irrigation followed by drought" cultivation management model, forming a highly efficient breeding method suitable for winter wheat. This method avoids dependence on a single known gene, instead achieving targeted improvement of water-saving, drought-resistant, and quality through synergistic screening of primer combinations and environmental adaptability breeding, providing a new technical path for wheat breeding in the arid North China region.

The application discloses a North China type coalfield collapse column exploration and evaluation method based on ground directional drilling technology, which comprises the following steps: determining the center position of the collapse column in the coal seam floor contour map within the three-dimensional seismic delineation as the opening position of the main hole for exploration; determining the number, direction and horizontal displacement of the directional branch holes for collapse column exploration; calculating the distance between the coal seam floor and the Ordovician limestone aquifer and the water head height of the Ordovician limestone aquifer; calculating the safety aquifer thickness M of the coal seam floor; calculating the final hole depth of the main hole for collapse column exploration and the final hole elevation of the branch hole; determining the deflecting radius and trajectory of the branch hole; performing the main hole construction, determining the top boundary position of the collapse column according to the core and performing the hydrogeological test; performing the branch hole construction, determining the upper interface and the side interface of the collapse column and performing the hydrogeological test; delineating the spatial form of the collapse column and analyzing and evaluating the water enrichment and water conductivity of the collapse column. The application provides a scientific basis for the water prevention and control design of the mining field and the mine, and ensures the safe and efficient production of the mine.