40 results about "Salt resistance" patented technology

This utility model belongs to the field of oil and gas extraction technology, and discloses a measuring device and a rotational rheometer for detecting the salt resistance of crosslinking agents. The measuring device for detecting the salt resistance of crosslinking agents includes a housing, a cover, a rotating assembly, and a liquid injection assembly. The housing is fitted into a supporting groove and has a communicating receiving chamber and a liquid injection channel, the liquid injection channel being located on the side wall of the housing. The cover is detachably fastened to the housing and has a through hole. The rotating assembly is located in the receiving chamber, and one end is rotatably mounted vertically in the through hole. The rotating assembly is connected to the output end of the rotational drive mechanism of the rotational rheometer. The liquid injection assembly includes a storage container, a liquid injection pipeline, and a liquid injection mechanism. One end of the liquid injection pipeline is connected to the storage container, and the other end is connected to the liquid injection channel. The storage container is used to store brine, and the liquid injection mechanism is located on the liquid injection pipeline. Using this measuring device to detect fracturing fluids can reduce the workload of workers and improve work efficiency.

The invention provides a thickening agent for self-crosslinking slick water, a preparation method of the thickening agent and fracturing fluid, and belongs to the technical field of oil exploitation. Comprising a temperature-resistant, shear-resistant and salt-resistant polymer, a synergist, a salt-resistant component, a structure regulator, a suspending agent and an emulsifier. The prepared thickening agent for the self-crosslinking slick water is good in temperature resistance, salt resistance and shearing resistance, has good tackifying and dissolving performance and good sand carrying and thickening effects, is low in damage rate to a rock core after gel breaking, can be widely applied to fracturing transformation of low-permeability and ultra-low-permeability reservoirs, and has a good application prospect. The prepared fracturing fluid integrates the functions of drag reduction, sand carrying, fracture forming, swelling prevention, discharge aiding, low damage and the like, and has a wide application prospect.

The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, methods, and workflows that include salt active nucleases (e.g., salt active nuclease variants) with desirable properties including, for example, salt tolerance.

The invention relates to application of P (NVP-co-MAA) in preparation of a pH and temperature dual-regulated switch type emulsion, and belongs to the technical field of emulsion preparation. According to the invention, P (NVP-co-MAA) is mixed with oil and water to obtain the emulsion, and the emulsion is found to be sensitive to pH and temperature, so that the emulsion can be used as a pH and temperature dual-regulated switch type emulsion. In addition, the switch type emulsion provided by the invention has good stability, still keeps an emulsified state under the NaCl concentration of 0.1 mol / L, and has good salt tolerance; when the pH is 5-6, the emulsion state can be maintained for at least 21 days, and the long-term stability is realized; and at least 15 times of emulsification-demulsification circulation can be carried out, and the circulation stability is good. The invention provides a new thought for the preparation of the switch type emulsion, and has a wide application prospect.

The invention relates to the technical field of agricultural transgenosis, and particularly discloses application of a GhDBB28 gene in regulating and controlling salt tolerance of upland cotton plants, and the nucleotide sequence of the GhDBB28 gene is as shown in SEQ ID NO.1; the sensitivity of upland cotton plants to salt is improved by silencing the GhDBB28 gene. The activity of an anti-oxidation system in the upland cotton plant is reduced through silencing of GhDBB28, so that the plant is more sensitive to salt stress, and a scientific basis is provided for constructing a new plant type of a salt-sensitive transgenic upland cotton plant.

The invention discloses a drag reducer for brine-resistant well cementation. The drag reducer comprises 45-60 parts by weight of a polycarboxylic acid polymer, 35-45 parts by weight of an aliphatic polymer and 5-10 parts by weight of a naphthalene sulfonic acid dispersant. The brine-resistant well cementation drag reducer provided by the invention is formed by mixing a plurality of polycarboxylic acid drag reducers, aliphatic drag reducers and naphthalene drag reducers which have dispersion effects, has the advantage of good dispersion at both high temperature and low temperature, and has a good salt-resistant effect; compared with the conventional drag reducer, the drag reducer provided by the invention has the advantages that the addition amount is greatly reduced, the efficiency is high, and the dispersing performance of a system is excellent; meanwhile, the rheological property is good, and the characteristic of sodium chloride brine resistance is also achieved; the drag reducer provided by the invention has broad spectrum performance and can be applied to conventional cement slurry, salt-containing cement slurry and high-density cement slurry systems.

The present application relates to a kind of double-core paper diaper water-absorbing material and its preparation method, belong to baby paper diaper field, the double-core paper diaper water-absorbing material includes acrylic acid 55-67 parts, salt-resistant anti-bonding auxiliary agent 10-14 parts, ammonium persulfate 1.4-1.7 parts, sodium sulfite 0.7-1.0 parts, glycerol 1-2.5 parts, aluminium sulfate 0.5-0.9 parts and ethanol 16-26 parts according to weight parts.The water-absorbing material is composed of water-absorbing core and coating layer.Amide group in water-absorbing core water-absorbing capacity is less affected by salt solution, after water absorption, water-absorbing core internal macromolecular network ionizes into ion pair, due to the difference between internal and external ion concentration produces greater osmotic pressure, can effectively improve the salt resistance and water-absorbing speed of water-absorbing material;The formation of coating layer further improves the salt resistance of water-absorbing material, and the gel strength of this coating layer is larger after water absorption, to avoid bonding agglomeration.

The application discloses a salt-resistant water-non-dispersible material for oil well cement and a preparation method thereof. The salt-resistant water-non-dispersible material for oil well cement comprises the following components in the form of weight parts: 100 parts of N,N-dimethyl acrylamide, 3-6 parts of acrylic acid, 10-20 parts of 2-acrylamide-2-methylpropanesulfonic acid, 5-15 parts of a cyclic monomer, 0.1-0.4 parts of a branching agent, 5-15 parts of a cationic monomer, 0.3-3 parts of a crosslinking agent and 400-500 parts of water. The salt-resistant water-non-dispersible material prepared by the application has good salt resistance, can significantly improve the anti-dispersion performance of the cement slurry when used in a salt water and sea water cement slurry system with a NaCl content of 18 wt% or less, and has excellent rheological performance.

The invention belongs to the technical field of plant breeding, and particularly relates to application of a GhPEP72 gene in regulation and control of plant salt tolerance. The nucleotide sequence of the GhPEP72 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein expressed by the GhPEP72 gene is as shown in SEQ ID NO. 2. A research result proves that the growth inhibition of a GhPEP72 gene silencing plant under salt stress treatment is more obvious, the salt damage characteristic is more obvious, sodium ion accumulation in roots, stems and leaves is increased, the potassium ion content is reduced, the ratio of sodium ions to potassium ions in the leaves is increased, and the GhPEP72 gene silencing plant is more sensitive to salt stress; compared with wild arabidopsis thaliana under salt stress treatment, the overground part of a GhPEP72 gene overexpression transgenic homozygous plant grows more vigorously, the number of lateral roots of the underground part is larger, the lateral roots are longer, and the GhPEP72 gene has good stress resistance, so that the GhPEP72 gene plays a positive regulation role in regulating plant salt stress.

The present application relates to a kind of anti-salt thickening agent and its preparation method and application, belong to the technical field of oil fracturing fluid processing.Solve the technical problems of low economic benefit, high cost and big environmental protection pressure in prior art that fracturing flowback fluid must be treated before being reused, such as metal ions, solid impurities and the like.The anti-salt thickening agent of the present application, by weight, raw materials include acrylamide 53-60 parts, acrylic acid 45-55 parts, 2-acrylamide-2-methylpropane sulfonic acid 1.5-2 parts, styrene 0.1-1 part and modified polyethylene glycol 3-6 parts.The anti-salt thickening agent, the introduced sulfonic acid group, benzene ring and tannic acid, have excellent salt resistance and stability, can resist fracturing flowback fluid with total salinity ≤1wppm, calcium and magnesium ion concentration ≤500ppm, and fracturing flowback fluid with iron ion concentration within 50ppm, providing a basis for direct use of fracturing flowback fluid.

[Problem] There is provided an inorganic oxide particle-containing composition exhibiting excellent dispersion stability of inorganic oxide particles and excellent salt tolerance. [Solution] An inorganic oxide particle-containing composition, wherein, in image analysis of a frozen sample by cryo-transmission electron microscope observation, the sample being prepared by adding the inorganic oxide particle-containing composition to 4% by mass of salt water such that the concentration of inorganic oxide particles is 0.5% by mass, and rapidly freezing with liquefied ethane, a coefficient of variation, which is a value obtained by dividing a standard deviation of areas of Voronoi partition regions based on an inorganic oxide particle distribution in a region of interest (ROI) by the arithmetic mean of the areas, is 0.1 to 0.7.

This invention relates to the field of rice genetic engineering, specifically providing the application of the OsMT9 protein and its encoding gene in regulating rice salt tolerance. This invention designs a specific targeting... OsMT9 The sgRNA in the coding region of the gene (target sequences shown in SEQ ID NO.4 and SEQ ID NO.5) was extracted, and a gene editing vector was constructed. The gene was then transformed into rice Zhonghua 11 using Agrobacterium-mediated transformation, yielding... OsMT9 Mutants with loss of gene function, such as mutants 22-1 and 23-15. Salt stress experiments showed that the survival rate of these mutants (approximately 43%) was significantly higher than that of the wild type (approximately 6%). This invention provides new gene targets and valuable germplasm resources for salt-tolerant rice breeding, and is of great significance for the utilization of saline-alkali land.

This invention relates to the field of flow analyzer technology, and particularly to a method for identifying the salt tolerance of wheat based on an AA3 type flow analyzer. The method includes: Step S1, obtaining a number of wheat samples to be tested; Step S2, preparing sodium ion and potassium ion standard working solutions respectively; Step S3, injecting each standard working solution into a continuous flow analyzer and measuring the sodium and potassium ion standard working curves using a flame photometer; Step S4, constructing the detection parameters for the samples to be tested; Step S5, injecting the samples into a continuous flow analyzer and measuring the absorbance of each standard stock solution using a flame photometer; Step S6, analyzing the salt tolerance of wheat based on the measured sodium and potassium ion concentrations of the samples to be tested. This invention has advantages such as continuous testing of batches of samples, fast analysis speed, saving manpower and resources, high accuracy, and strong anti-interference ability.

The application relates to the field of agricultural transgenic technology, and particularly discloses GhDBB28 application of a gene in regulating salt tolerance of upland cotton plants, the gene GhDBB28 The nucleotide sequence of the gene is shown as SEQ ID NO. 1; silencing the gene GhDBB28 increases the salt sensitivity of the upland cotton plants. The application reduces the activity of an antioxidant system in the upland cotton plants by silencing the gene GhDBB28 , so that the plants are more sensitive to salt stress, and a scientific basis is provided for constructing a transgenic upland cotton plant with new salt-sensitivity.

The invention relates to the technical field of soil remediation, in particular to salt resistance and alkali reduction equipment suitable for saline-alkali soil, comprising a mounting rack and a feeding assembly mounted on the mounting rack; the device further comprises a power assembly. The mounting frame is connected with a power assembly; the power assembly is connected with a soil dividing plate, and the soil dividing plate is hollow; the soil separating plate is connected with a conveying assembly for continuously conveying materials of the salt isolating layer; a guide plate is arranged on each of the two sides of the soil dividing plate; the lower part of the soil dividing plate is fixedly connected with a mounting cylinder; and two outer arc plates which are symmetrically distributed are rotationally connected in the mounting cylinder. The two outer arc plates are pushed by the two connecting rods to be unfolded towards the opposite sides by taking the mounting cylinder as the center, a combined shape is changed into an acute angle shape, powdery soil on the outer sides of the two outer arc plates is pushed away towards the two sides, and meanwhile, the two outer arc plates drive the corresponding fan-shaped elastic pieces to move out of the fan-shaped grooves, so that the powdery soil can be removed. The unfolded outer arc plates and the fan-shaped elastic pieces are separated to form a space for laying corn straw on the lower portion of the plough layer.

To provide means capable of imparting heat tolerance or salt tolerance to plants.SOLUTION: The present invention provides a heat tolerance or salt tolerance improving agent for plants, comprising acetic acid or a salt thereof, or a solvate thereof. A method for improving the heat tolerance or salt tolerance of a plant comprises applying acetic acid or a salt thereof, or a solvate thereof to a plant, a material for application to the plant, or soil, a medium, or a culture solution where the plant grows.SELECTED DRAWING: None

The invention relates to the technical field of plant genetic engineering, and particularly provides application of an AHP2 gene in regulation and control of salt tolerance, plant height, tiller number and grain traits of rice. The invention discloses the multiple-effect function of the AHP2 gene for the first time. Experiments show that the AHP2 gene is knocked out, so that the sensitivity of a rice plant to salt stress is remarkably increased, the survival rate in the seedling stage is reduced, the plant height is reduced, the tiller number is reduced, and the grain length, the grain width, the grain thickness and the thousand grain weight of grains are remarkably reduced; and overexpression of the AHP2 gene can significantly improve the salt tolerance of rice in the seedling stage, reduce ion toxicity under salt stress, and maintain the plant height, tillering and grain traits of the wild type. The AHP2 gene provided by the invention provides a key gene resource and an effective technical approach for realizing collaborative improvement of a plurality of important agronomic traits, and has an important value for coping with soil salinization and cultivating stable and high-yield rice varieties.

To provide a gel composition for external use which forms a gel having excellent salt resistance, acid resistance and alkali resistance and forms a film having no stickiness and excellent rubbing resistance even under high humidity, and to provide a cosmetic containing the same.SOLUTION: The gel composition for external use contains a copolymer (A) containing a constitutional unit represented by formula (a), a constitutional unit represented by formula (b) and a constitutional unit represented by formula (c), and a copolymer (B) containing a constitutional unit represented by formula (a) and a constitutional unit represented by formula (c) or a constitutional unit represented by formula (d). (a) - (CH2-C (R1) - C (= O) - OCH2CH2O - P (= O) (O -) - O (CH2) 2-N + (CH3) 3) - (b) - (CH2-C (R2) - C (= O) - O-X) - (c) - (CH2-C (R3) - C (= O) - O-X) - (d) - (- C () - C (= O) - - CH (OH) - - N + () 3) - CH3 R4 CH2 CH2 OCH2 R5 SELECTED DRAWING: None

The application relates to the technical field of plant genetic engineering, and particularly provides an application of a gene in regulating rice salt tolerance, plant height, tiller number and grain traits. AHP2 The application discloses a multiplicity function of the gene. Experiments show that knocking out the gene leads to a significant increase in salt stress sensitivity of rice plants, a reduction in survival rate at the seedling stage, a reduction in plant height, a reduction in tiller number, and a significant reduction in grain length, grain width, grain thickness and 1000-grain weight; and overexpression of the gene can significantly improve salt tolerance of rice seedlings at the seedling stage, reduce ion toxicity under salt stress, and maintain the plant height, tiller and grain traits of the wild type. AHP2 The application provides a key gene resource and an effective technical approach for realizing the synergistic improvement of multiple important agronomic traits, and has important values for coping with soil salinization and cultivating stable and high-yield rice varieties. AHP2 AHP2 AHP2 ​​​

The invention relates to a high-temperature-resistant high-salt filtrate reducer PAHS-ATP and a preparation method and application thereof, the preparation method comprises the following steps: 1) preparing three monomers of AM, AMPS and SSS into an aqueous solution, and adjusting the pH value of the aqueous solution to 7; adding an initiator under the protection of nitrogen, and reacting at 75 DEG C for 6 hours to obtain a PAHS polymer solution; 2) mixing ATP with an HCl solution with the concentration of 5%, acidizing, washing the acidized ATP to be neutral, and dispersing in ethanol to obtain a reaction solution; adding KH570 into the reaction liquid, adjusting the pH value of the reaction liquid to 8-9, and carrying out reflux reaction on the reaction liquid at 70 DEG C for 6 hours; after the reaction is finished, centrifuging, drying and grinding to obtain KH570-ATP powder; and (3) adding KH570-ATP powder into the PAHS polymer solution, and continuously reacting for 6 hours at 75 DEG C to obtain the filtrate reducer PAHS-ATP. The filtrate reducer prepared by the invention has excellent high-temperature resistance and salt resistance, can effectively control the filtration loss of high-temperature and high-salt drilling fluid and maintain rheological stability, and is particularly suitable for ultra-deep oil and gas drilling.

The invention provides a salt-resistant thickening agent for fracturing as well as a preparation method and application thereof, and belongs to the technical field of reservoir transformation. The thickening agent is a quinary copolymer, and the structure of the copolymer is as shown in a formula I, wherein m, n, o, p and q are the mole percentages of the structural units respectively; n = 10%-15%, m + n = 75%, p + q + o = 25%, p = 20%, and q = 2%-4%. The thickening agent disclosed by the invention has good salt resistance, instant dissolving performance and resistance reducing performance, can improve the application range of the hypersalinity fracturing fluid, improves resistance reducing, crack forming and sand carrying capacities, and is suitable for the hypersalinity fracturing fluid.

The application provides a gold nanoparticle with enhanced stability and a preparation method and application thereof, and belongs to the technical field of gold nanoparticles. The gold nanoparticle with enhanced stability provided by the application is modified on the surface with a hairpin DNA; the nucleotide sequence of the hairpin DNA is shown as SEQ ID NO. 1. The gold nanoparticle of the application is modified by the hairpin DNA, and the stability is significantly improved, specifically including tolerance to a strong acid environment, stable existence in an environment with a pH of 2 or more, and a substantial improvement in salt tolerance, tolerance to 100 mM Mg 2+ of the above ion strength.

The invention belongs to the technical field of oil and gas field fracturing fluid functional materials, and discloses a high-salt-resistant and high-temperature-resistant instant associative thickening agent for fracturing fluid and a preparation method of the high-salt-resistant and high-temperature-resistant instant associative thickening agent. The thickening agent is formed by synergistically associating a ternary hydrophilic functional component, a hydroxyethyl substituted imidazole ring salt-tolerant functional component, a three-component amphiphilic association functional component and a double-grafting modified graphene oxide functional component through physical and chemical interaction. The quadruple synergistic dynamic three-dimensional association network is constructed through carboxyl-sulfonyl hydrogen bonds, long-chain alkyl-polyoxypropylene chain hydrophobic association, graphene oxide sheet layer pi-pi accumulation and double grafting group dynamic interaction. The preparation method adopts a mode of combining component compounding and synergistic association, and the product has excellent high-salt resistance, high-temperature resistance stability, rapid solubility and continuous thickening capacity, is adaptive to a complex underground environment of a deep oil and gas field, and meets the comprehensive performance requirements of fracturing construction on functional materials.

The present application relates to a kind of temperature and salt resistant imbibition agent, by the Mannich reaction of p-xylylenediamine with formaldehyde, formic acid is synthesized to obtain tetramethyl binary tertiary amine intermediate;The intermediate is reacted with alpha-bromine carboxylic acid under alkaline conditions to generate gemini betaine surfactant.Gemini betaine surfactant is compounded with one or two of non-ionic surfactant and small molecule alcohol, and temperature and salt resistant imbibition agent is prepared.The imbibition agent has the characteristics of easy entering matrix channel, anti-absorption, strong wetting reversal ability, temperature resistance can reach 150 DEG C, salt resistance can reach 15×10 4 mg / L, can meet the technical requirements of oil reservoir temperature ≤150 DEG C, salinity ≤15×10 4 mg / L low permeability oil reservoir imbibition oil recovery technology.

The present application relates to the technical field of molecular marker development and molecular marker assisted breeding, and specifically discloses a KASP molecular marker related to salt tolerance of sorghum, a primer and application thereof.The KASP molecular marker is ST1.1 as shown in SEQ ID NO.1, and the base at 100bp of the sequence shown in SEQ ID NO.1 has T / C polymorphism.The KASP molecular marker ST1.1 provided by the present application is used for identifying salt tolerance of sorghum, and through KASP genotyping detection, the genotype of a sample with strong FAM fluorescence signal is T / T, which is salt-tolerant germplasm;the genotype of a sample with strong HEX fluorescence signal is C / C, which is salt-sensitive germplasm.

The application discloses a kind of salt-resistant antibacterial high interfacial activity gemini foam displacement agent and preparation method and application, long-chain alkyl tertiary amine or N-alkyl imidazole and 1,4-dibromo-2-butene are reacted in ethanol to obtain gemini intermediate, then gemini intermediate is reacted with anhydrous sodium sulfite in ionic liquid, and gemini surfactant is obtained.The preparation method of foam displacement agent of the application utilizes ionic liquid as dissolving to realize homogeneous sulfonation, greatly reduces the dosage of sodium sulfite, and the process is simple, easy to operate, short, and high in yield.Compared with traditional gemini foam displacement agent, the foam displacement agent of the application presents a typical cation / zwitterion hybrid structure, and has higher bactericidal and bacteriostatic properties, salt resistance, interfacial activity and foam performance;At the same time, the adsorption of traditional cationic gemini surfactant on the surface of rock is greatly reduced.It provides a new solution for high-salt oil reservoir oil displacement.

The application discloses a high-salt-resistant foaming agent, which can meet the requirements of drilling construction in complex strata by coping with harsh geological conditions in the process of foam drilling and multiple requirements of foam inhibition and foaming capacity. The foaming agent is composed of the following components in percentage by mass: 12-60wt% main foaming agent, 10-30wt% auxiliary foaming agent, and the balance of water. The main foaming agent is a betaine zwitterionic surfactant containing a polyether chain segment, and the auxiliary foaming agent is an amino propionic acid type zwitterionic surfactant. The foaming agent system prepared by the application has the advantages of temperature resistance of 140 DEG C, salt resistance of 360000mg / L NaCl, excellent foaming effect, good salt resistance and good inhibition effect on hydration of mud shale.

This invention provides a medium-phase microemulsion, its preparation method, and its application. The medium-phase microemulsion comprises a surfactant, surfactant auxiliaries, inorganic salts, crude oil, and water. The surfactant is an anionic nonionic surfactant, including the surfactant APEG-LY-SSS, with the following structural formula: [Formula omitted for brevity]. Wherein, 5≤x≤15, 2≤y≤20, 4≤z≤10, R1 is H or CH3, and R2 is a C2-C18 alkyl, hydroxyethyl, hydroxypropyl, or methoxy group. The medium-phase microemulsion prepared by this invention exhibits excellent anti-adsorption properties, strong temperature resistance, and strong salt resistance, showing broad prospects for industrial application.