Micro-ecological preparation and application thereof

A technology of compound microecology and preparation, applied in the direction of application, microbiology, microbiology, etc., can solve the problems of affecting the host, restricting the wide application of microecological preparations, and difficult to meet the treatment needs, so as to improve immunity and disease resistance, and improve animal health. Product quality and the effect of improving feed utilization efficiency

Active Publication Date: 2009-11-18
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] At present, the main quality problems of the following aspects exist in the probiotics on the market: (1) the content of viable bacteria is low, and studies have shown that if the concentration of a kind of bacteria in the cecal content is as low as 107 / g, then the The enzymes and metabolites produced by the bacteria are not enough to affect the host, and it is difficult to meet the needs of treatment; (2) the water content is too high, which is one of the important factors affecting the quality of microecological preparations; (3) not resistant to antibiotics; (4) resistant to gastric acid and bile salts are unstable, so that only a few strains are still active after entering the intestinal tract, which does not reach the number of viable bacteria required to function
Due to these quality problems of probiotics, it affects its application effect on animals and limits the wide application of probiotics

Method used

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  • Micro-ecological preparation and application thereof
  • Micro-ecological preparation and application thereof
  • Micro-ecological preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1, the separation, screening, selection and identification of excellent bacterial strains

[0062] 1) Bacterial enrichment culture: Inoculate fresh feces and intestinal content samples of healthy animals (pigs or poultry) into BPY liquid medium, and culture and proliferate at a constant temperature of 37°C for 48 hours;

[0063] 2) Isolation and purification of Bacillus licheniformis: step 1) enrichment culture in a 100°C water bath for 5 minutes, then streak culture on a BPY medium plate, pick out each colony with characteristic morphology, streak culture and separation, Until it is a pure colony, the Gram staining microscope is used to inoculate the isolated suspected pure colony into the inclined plane of the test tube to proliferate, and then store it in a refrigerator at 4°C until use;

[0064]Colony morphology: the surface of the colony is rough, dull, opaque milky white, with irregular edges, 2-3mm in diameter, and becomes reddish-brown as the culture ...

Embodiment 2、3

[0070] Stress resistance and biological performance measurement of embodiment 2, 3 excellent bacterial strains

[0071] Three strains: Bacillus licheniformis CGMCC No.2383, Enterococcus faecalis CGMCC No.2386, Saccharomyces cerevisiae CGMCC No.2388 Determination of biological properties and stress resistance:

[0072] 1) Preparation of the culture of Bacillus licheniformis CGMCC No.2383: Inoculate the slant strains stored in the refrigerator into BPY seed medium for activation, and cultivate at 37° C. and 200 rpm for 18 hours to obtain a culture with a spore rate of more than 95%;

[0073] 2) Preparation of Enterococcus faecalis CGMCC No.2386 culture: inoculate the slant bacteria stored in the refrigerator into the MRS seed medium for activation, and culture at 35°C and 100rpm for 16h to obtain the product;

[0074] 3) Preparation of Saccharomyces cerevisiae CGMCC No.2388 culture: inoculate the slant bacteria stored in the refrigerator into No. 98 seed medium for activation, a...

Embodiment 3

[0104] Embodiment 3, the preparation of Bacillus licheniformis CGMCC No.2383 bacteria powder

[0105] 1) Plate culture rejuvenation: Inoculate the Bacillus licheniformis strain on the BPY plate medium, culture at 37°C for 24 hours, rejuvenate the Bacillus licheniformis, and form a single colony, pick a single colony and inoculate it on the slant medium, Incubate at 37°C for 36 hours;

[0106] 2) Preparation of first-class seeds: transfer the Bacillus subtilis cultured in step 1) to the eggplant bottle slant medium, and cultivate them at 37° C. for 12 hours to make them in the mid-late logarithmic period to obtain first-class seeds;

[0107] Described slant medium is BPY solid medium;

[0108] 3) Preparation of secondary seeds: make bacterial suspension from the primary seeds prepared in step 2) with sterile water, and inoculate into 1.6M 3 2M of BPY seed medium 3 In the seed tank, the temperature is 37°C, the rotation speed is 200rpm, the tank pressure is 0.05Mpa, and the ...

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Abstract

The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.

Description

technical field [0001] The invention relates to a composite microecological preparation, in particular to a composite microecological preparation containing multiple probiotics and its application, belonging to the technical field of probiotics. Background technique [0002] At present, antibiotics are still used in the prevention and treatment of animal diseases, but due to the long-term and large-scale use of antibiotics, various disadvantages such as drug resistance, flora imbalance, decreased immunity, high disease recurrence rate, and drug residues are becoming more and more serious. Seriously endanger animal organisms and human health. At present, countries around the world have adopted measures to ban or restrict the use of antibiotics, such as: the European Union has banned the use of antibiotics in 2006; the World Health Organization (WTO) has proposed "global principles for reducing the use of antibiotics in food animals"; The "Feed Safety Project" was launched, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/18A23K1/16C12R1/10C12R1/125C12R1/01C12R1/23C12R1/865A23K10/18
Inventor 孙占敏张玳华白玉卿姚琨贾秋英刘宇赵军赵雁青莫云
Owner BEIJING DABEINONG TECH GRP CO LTD
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