The invention provides a novel anti-counterfeit mark. The novel anti-counterfeit mark comprises an anti-counterfeit mark base and an invisible anti-counterfeit pattern, wherein the regional difference is formed through micro-nano materials and the anti-counterfeit mark base, so that the invisible anti-counterfeit pattern is formed, the surface of the anti-counterfeit mark base comprises an invisible anti-counterfeit pattern area and a non-pattern area, the invisible anti-counterfeit pattern area or the non-pattern area is composed of a film layer formed on the surface of the anti-counterfeit mark base through micro-nano materials, and the micro-nano materials include organic particles, inorganic particles, photoresist, photosensitive resin, metal, alloy, ceramic, polymer, active micromolecules, an oxide, a sulfide, a nitride and a carbide or a binary compound or polycompound composed of III-V or II-IV or IV-VI elements. According to the novel anti-counterfeit mark, the invisible anti-counterfeit pattern can appear when the anti-counterfeit mark is located at the position of an air blowing opening or encounters with water or steam, the invisible anti-counterfeit pattern can become invisible after water is volatilized, and the anti-counterfeit mark can be used for counterfeit prevention in the fields such as the medicine field, the food safety field and the financial field. The invention further provides a manufacturing method of the novel anti-counterfeit mark.