The invention relates to a sterilization composition containing pyraoxystrobin and JS399-19. The effective ingredients, namely the pyraoxystrobin and the JS399-19, are compounded, wherein the mass ratio of the pyraoxystrobin to the JS399-19 is (1-80): (80-1); the total mass of the effective ingredients, namely the pyraoxystrobin and the JS399-19, is 1-80% of the total mass of the whole preparation, and the remnants are allowable and acceptable auxiliary components in pesticide. The sterilization composition can be prepared into following pesticide formulations: missible oil, suspending agents, wettable powder, water dispersible granule, emulsion in water and micro-emulsion, can be used for preventing and treating wheat scab, fusarium wilt of cotton, banana panama disease, rice bakanae disease and watermelon fusarium wilt, and has excellent control effects on cucumber downy mildew, grape downy mildew, tomato late blight, cucumber anthracnose and cucumber powdery mildew.