The invention discloses an application of camellia oleifera extract in antiseptic and fresh preservation. The camellia oleifera extract can be applied to post-harvest fruit freshness preservation, and the post-harvest pathogens to fruit include Penicillium italicum Wehmer, Penicillium digitatum Sacc., Geotrichum candidum Link ), banana crown rot (Colletotrichum musae spp), banana anthracnose (Colletorchum musae), litchi downy mildew (Peronophythora litchi), litchi anthracnose (Colletotrichum gloeosporioides Penz.), loquat anthracnose (Colletotrichum acutatum Simmonds), loquat Alternaria Tenuis Nees, Pestalotiopsis eriobotryfolia, etc. have good control effects, and have good fresh-keeping activities on various fruits such as citrus, litchi, banana, loquat, longan, and mango. According to this characteristic of camellia oleifera, an efficient and pollution-free green plant-source fruit preservative can be developed, which has significant social and economic benefits.