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Strain of Paenibacillus jejuni capable of inhibiting anthrax and Fusarium and application thereof

A technology of Bacillus and Glycystis anthracis, which is applied in the directions of application, chemicals for biological control, bacteria, etc., can solve problems such as the absence of Paenibacillus jemila, and achieves low production cost, good control effect, and environmental protection. friendly effect

Active Publication Date: 2019-01-25
CHINA KINGDOM AGRITECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is currently no relevant report on the use of Paenibacillus jemila for the biological control of plant fungal diseases

Method used

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  • Strain of Paenibacillus jejuni capable of inhibiting anthrax and Fusarium and application thereof
  • Strain of Paenibacillus jejuni capable of inhibiting anthrax and Fusarium and application thereof
  • Strain of Paenibacillus jejuni capable of inhibiting anthrax and Fusarium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Isolation and screening of Paenibacillus jemiella ZDC-04

[0019] (1) Isolation method of Paenibacillus jemila ZDC-04

[0020] Paenibacillus jemira ZDC-04 of the present invention is isolated from the East China Sea seabed sediment (120.6 ° E, 34 ° N), and the depth of water is 17 meters. It is separated and obtained by the dilution coating method. The specific method is: take 10 g of the sea bottom sediment The sample was dissolved in 90mL sterile water, placed in a shaker and vibrated for 30min, so that the sample was fully mixed with water, and 10 -1 Diluent. Take 1ml of the above dilution into 9ml sterile water, shake well to obtain 10 -2 Diluent. the same way to get 10 -3 、10 -4 、10 -5 、10 -6 diluent. Take 10 respectively -4 、10 -5 、10 -6 100 μL of the diluted solution was poured into the poured LB plate, and the diluted solution was spread evenly with a spreader, and each concentration was repeated 3 times. Place them in an incubator at 30°C ...

Embodiment 2

[0026] The identification of embodiment 2 Paenibacillus jemira ZDC-04

[0027] (1) Morphology of the strain: the bacterium is long and rod-shaped, the size is (0.5-1.1) μm×(3.2-5.2) μm, it produces spores, and Gram staining is positive. When cultured on LB medium at 30°C for 48 hours, the colony was white, round, with raised edges.

[0028] (2) Molecular identification results

[0029] The 16S rRNA gene sequence determination result (SEQ-1) of this bacterial strain is as follows:

[0030]AAGCTTGCTTCTAATTAACCTAGCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCACAAGACAGGGATAACTACCGGAAACGGTAGCTAATACCCGATACATCCTTTTCCTGCATGGGAGAAGGAGGAAAGGCGGAGCAATCTGTCACTTGTGGATGGGCCTGCGGCGCATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGCCAGGGAAGAACGCTTGATAGAGTAACTGCTCTTGAAGTGACGGTACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTATTG...

Embodiment 3

[0032] Embodiment 3 Jamila Paenibacillus ZDC-04 fermentation process

[0033] 2216E medium: yeast extract 1g, peptone 5g, agar 15-20g, filtered seawater 1000mL, pH adjusted to 7.4-7.5; LB medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, agar 15 -20g, water 1000mL, pH adjusted to 7.0-7.2.

[0034] 2216E liquid medium: yeast extract 1g, peptone 5g, filtered seawater 1000mL, pH adjusted to 7.4-7.5; LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, water 1000mL, pH adjusted to 7.0-7.2.

[0035] Fermentation medium: 15g corn flour, 10g soybean meal, 8g bran, 6g sucrose, 5g calcium carbonate, 5g potassium dihydrogen phosphate, 2g sodium chloride, 1000mL water, adjust the pH to 6.5.

[0036] Fermentation method, comprises the following steps:

[0037] (1) Activate the Paenibacillus jemila ZDC-04 preserved at low temperature with 2216E medium or LB medium, and place it in a constant temperature incubator at 30°C for 48 hours;

[0038] (2) Transfer ...

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Abstract

The invention discloses a strain of Paenibacillus jejuni for inhibiting anthrax and Fusarium and an application thereof. A strain of Paenibacillus jamilae is isolated from the deep sea sediment, the strain was deposited at the General Microbial Center of the China Microbial Culture Collection Management Committee under accession number CGMCC No.16231 on August 8, 2018. The strain has broad-spectrum resistance to many plant pathogens, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by Colletotrichum gloeosporioides and vegetable wilt caused by Fusarium oxysporum.

Description

technical field [0001] The invention belongs to the field of biotechnology prevention and control, and in particular relates to a strain of Paenibacillus jemira and its application in inhibiting pathogenic fungi and preventing and controlling fungal diseases. Background technique [0002] Colletotrichum gloeosporioides Penz is the most widely distributed pathogen with the most hosts, which can damage fruit trees, vegetables, forest trees, etc. Apple anthracnose is an important disease that harms fruit. In diseased orchards, the general diseased fruit rate is about 30%, and the serious one can reach 50%-60%. In susceptible varieties, it can cause devastating losses. Citrus anthracnose is a disease that commonly occurs in citrus producing areas in my country. The disease mainly damages leaves, branches, flowers and fruits, causing a large number of fallen leaves, dead branches, and a large number of flower and fruit drop. The infected fruits continue to be damaged during stora...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 尉莹莹栾守业朱凤蒙
Owner CHINA KINGDOM AGRITECH QINGDAO
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