290 results about "Wilt disease" patented technology

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

The invention discloses a bacillus AH83 capable of resisting saline and alkaline and promoting growth and application thereof, and belongs to the technical field of microbiology and biology, the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495, and the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495. The AH83 provided by the invention is a new species of bacillus, can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the AH83 can survive in a highly saline-alkaline environment, can promote agronomic characters such as plant height, biomass, leaf area and root development in a corn seedling stage when applied in saline-alkaline soil, can also increase soil nutrients and enzyme activity content, and has a promoting effect on lavender seed germination. The strain is of great significance in improving crop yield and stress resistance and developing novel agricultural microbial agents.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention relates to the technical field of biology, in particular to application of PnPUB35 protein and a coding gene thereof to regulation and control of plant wilt resistance. The invention provides a Ubiquitin ligase PnPUB35 protein containing a U-box structural domain E3 and a gene PnPUB35 of the Ubiquitin ligase PnPUB35 related to resistance to fusarium wilt of pepper, and after the pepper is inoculated with fusarium solani, the expression quantity of the Ubiquitin ligase PnPUB35 protein at the root of the pepper is increased. The resistance of a pepper PnPUB35 silent plant to fusarium solani is weakened, and the resistance of an arabidopsis thaliana PnPUB35 overexpression plant to fusarium solani is enhanced. The PnPUB35 gene disclosed by the invention plays a positive regulation role in pepper wilt resistance, and can be used as an important candidate gene for pepper wilt resistance breeding.

The invention discloses biocontrol marine source penicillium and application thereof, and relates to the technical field of microorganisms. The biocontrol marine source penicillium is named as Penicillium sp.DSF059 and is preserved in the China Center for Type Culture Collection, and the preservation number of the biocontrol marine source penicillium is CCTCC NO: M 20232310. The invention discloses biocontrol marine-source penicillium. The biocontrol marine-source penicillium has effects on rubber fusarium wilt pathogenic bacteria, coconut gray leaf spot pathogenic bacteria, wheat scab pathogenic bacteria, cucumber phytophthora root rot pathogenic bacteria, avocado phytophthora root rot pathogenic bacteria, rubber anthracnose pathogenic bacteria, mango anthracnose pathogenic bacteria, sisal zebra stripe pathogenic bacteria, rubber red root pathogenic bacteria, bacillus subtilis and bacillus subtilis. The invention provides a new direction for the prevention and treatment of the tropical crop pathogenic bacteria, and has a very good development and application prospect, and the tropical crop pathogenic bacteria such as the pathogenic bacteria of the rubber brown root disease, the coffee anthracnose pathogenic bacteria, the stylosanthes guianensis anthracnose pathogenic bacteria and the banana anthracnose pathogenic bacteria can be prevented and treated.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses an InDel marker primer for identifying tomato spotted wilt susceptibility and application of the InDel marker primer, and belongs to the technical field of molecular biology. The InDel marker primer comprises InDc10221-F with the nucleotide sequence as shown in SEQ ID NO.1 and InDc10221-R with the nucleotide sequence as shown in SEQ ID NO.2, high-specificity accurate detection can be carried out on tomato varieties carrying the tomato spotted wilt susceptible gene SldnajG, the operation steps are simple, the interpretation mode is visual, and the method has a good application prospect in screening of tomato spotted wilt resistant tomato germplasm resources. A simple, rapid, economical and effective molecular tool is provided for screening out tomato spotted wilt susceptible varieties, the molecular tool can be applied to commercial tomato disease-resistant breeding and high-quality genetic improvement application research, and a new choice is provided for tomato spotted wilt disease-resistant breeding application.

Bacteriophages that comprise a tail fiber protein sequence comprising a C terminus host recognition site described by SEQ ID NO: 1 and which infect Ralstonia solanacearum which causes bacterial wilt disease are disclosed. Compositions comprising these bacteriophages may be employed as wilt control agents. Methods for preventing or treating diseases caused by Ralstonia solanacearum such as bacterial wilt disease are also disclosed. A bacterial wilt disease control method in a plant may include administering such bacteriophages to a plant or to a plant growth medium and can be effective against a wide variety of Ralstonia solanacearum strains. Nucleic acids encoding the C terminus host recognition site and amino acid sequences comprising the host recognition site are also disclosed.

The invention provides a pine wood nematode disease tree detection method and system based on image analysis, and relates to the technical field of image processing. The method comprises the following steps: acquiring a to-be-analyzed pine image acquired by an unmanned aerial vehicle, and determining a plurality of reference crown areas in the to-be-analyzed pine image; respectively carrying out diseased tree fading analysis and crown form analysis on each reference crown area, carrying out primary anomaly detection on the plurality of reference crown areas, and dividing to obtain a reference sample group and an abnormal sample group; performing clustering analysis based on environment distribution difference on the plurality of reference tree crown areas in the reference sample group to generate a plurality of environment influence tree crown groups; and determining an environment influence crown group to which each reference crown region belongs in the abnormal sample group, and carrying out secondary anomaly detection on the plurality of reference crown regions to obtain a pine wood nematode pest detection result of the to-be-analyzed pine image. According to the invention, precise detection of the pine wood nematode disease tree is realized.

The invention belongs to the technical field of microorganisms, and discloses a compound microbial agent and application thereof in crop disease control. According to the invention, two plant endophytes, namely trichoderma asperellum and trichoderma atroviride, are separated, the trichoderma asperellum is Trichoderma asperellum M11, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.40752; the trichoderma atroviride is Trichoderma atroviride T5, and the preservation number of the trichoderma atroviride is CGMCC (China General Microbiological Culture Collection Center) No. 22432. Fermentation liquor of two trichoderma strains and auxiliaries are prepared into the compound microbial agent, the compound microbial agent has excellent prevention and treatment effects on pepper phytophthora blight, cucumber fusarium wilt, watermelon fusarium wilt, cucumber damping off, tomato sesame spot, tomato early blight and tomato leaf mold, in addition, the compound microbial agent can promote growth and increase yield, the disease resistance of plants is improved, and the application prospect is wide.

The invention belongs to the technical field of biological control of cotton wilt, and specifically relates to the application of chalcone compounds in preventing and controlling cotton wilt and a method for preventing and controlling cotton wilt. The invention uses chalcone compounds as the only active ingredient, and plate antibacterial tests and pot tests confirm that chalcone can inhibit the growth of cotton wilt pathogens, reduce the disease index of cotton wilt, and prevent and control cotton wilt.

The invention discloses bacillus subtillis ZKY07 for preventing and controlling bacterial diseases of pachyrhizua angulatus and application of the bacillus subtillis ZKY07. According to the invention, a strain ZKY07 which has an antagonistic effect on two pathogenic bacteria of the Pachyrhizua angulatus bacterial wilt is screened from strains separated from dendrobium leaves and is used for biological control of the Pachyrhizua angulatus bacterial wilt, and the preservation number of bacillus subtillis ZKY07 is GDMCC No: 64618. Through determination, the ZKY07 has a good antagonistic effect on ralstonia solanacearum causing pogostemon cablin bacterial wilt, ralstonia solanacearum causing peanut bacterial wilt, ralstonia solanacearum causing tomato bacterial wilt, Klebsiella variicola causing banana sheath rot, fusarium asiaticum causing peanut fruit rot and sclerotium rolfsii causing peanut southern blight. The strain ZKY07 has the effects of producing protease, producing cellulase, dissolving potassium, dissolving organophosphorus and fixing nitrogen. The invention provides an excellent biological control means for the bacterial wilt of the pueraria thomsonii.

The invention discloses a nano mimetic enzyme as well as a preparation method and application thereof. The nano-mimic is of a nano-cloud structure formed by stacking irregular spheres and rods, and the nano-cloud structure is a one-dimensional, two-dimensional or three-dimensional system. The preparation method of the nano mimic enzyme comprises the following steps: 1) dissolving a copper salt and a manganese salt in deionized water to obtain a mixed solution; 2) adding hydrogen peroxide into the mixed solution to obtain a Cu < 2 + > / Mn < 3 + > mixed solution A; 3) adding the alkaline solution B into the Cu < 2 + > / Mn < 3 + > mixed solution A, stirring and mixing to obtain a mixed brown turbid solution, and standing and aging; and (4) precipitating and separating the aged turbid liquid in the step (3), cleaning, drying and grinding to obtain the nano mimic enzyme. The nano mimetic enzyme suspension is applied to watermelon seed priming. The nano enzyme mimetic suspension disclosed by the invention is applied to preparation of antibacterial drugs. The nano mimic enzyme can promote seed germination and growth, and has a remarkable inhibition effect on watermelon bacterial germs, namely fruit blotch germs or watermelon fungal germs, namely wilt germs.

The invention relates to the field of potato planting, in particular to a preparation method of a safe seed dressing agent for seed potatoes and a seed dressing method.The potato seed dressing agent is prepared by mixing a calcium magnesium phosphate fertilizer, 50% carbendazim wettable powder, 3% Zhongshengmycin wettable powder, 80% mancozeb wettable powder and a slow release component, and the seed potatoes are cut into blocks for seed dressing. The method can safely and efficiently prevent and treat diseases such as soft rot, bacterial wilt and wilt of potatoes, reduces the rotting rate of potato blocks, improves the emergence rate, yield and quality of potatoes, and is simple to operate, easy to master and suitable for large-scale popularization and application.

The utility model belongs to the technical field of forestry epidemic disease detection, and discloses a sampling device for pine wood nematode disease detection, which comprises a U-shaped frame, one side of the U-shaped frame is fixedly connected with a support, one end of a push rod is fixedly connected with a push plate, the other end of the push rod is fixedly connected with a motor, the tail end of a rotor of the motor is coaxially and fixedly connected with a drill bit, and the drill bit is fixedly connected with the support. The clamping assembly comprises a one-way lead screw rotationally connected to one side of the U-shaped frame, the outer portion of the one-way lead screw is in threaded connection with a moving block, clamping plates are symmetrically arranged on the inner side face of the U-shaped frame, a cross rod is fixedly connected to one side of each clamping plate, and the cross rods are in sliding connection with the U-shaped frame; by means of the pine tree sampling device, the problems that due to the fact that the stability during drilling and sampling is difficult to guarantee, the pine tree sampling efficiency is reduced, and damage to pine trees is easily increased are effectively solved.

The invention discloses a sesame fusarium wilt identification method based on a molecular marker, and relates to the technical field of phytopathology, the identification method comprises the following steps: S100, RNA extraction and purification: extracting total DNA and protein pollution RNA from a sesame sample to be detected, purifying the extracted RNA by using an efficient RNA extraction kit, removing existing impurities and inhibitors, and determining the content of the protein pollution RNA in the sesame sample to be detected; according to the method, specific long-chain non-coding RNA is adopted as a molecular marker, the molecular marker has high specificity and can accurately distinguish the sesame wilt from other diseases and healthy plants, the probability of misjudgment can be greatly reduced through data analysis, and the detection accuracy is improved. The specific long-chain non-coding RNA has the advantages that the specific long-chain non-coding RNA can be used for detecting the sesame fusarium wilt, reliable guarantee is provided for accurate identification of the sesame fusarium wilt, meanwhile, the specific long-chain non-coding RNA has good stability under different environmental conditions, and detection of a large number of samples can be completed within a short time through PCR amplification and high-throughput sequencing.

The invention relates to the technical field of soil improvement, and discloses a conditioning method for banana continuous cropping obstacle sick soil. The conditioning method for the banana continuous cropping obstacle diseased soil comprises the following steps that banana root systems and banana straw in the banana continuous cropping obstacle diseased soil are removed and smashed, and banana straw particles are obtained; applying the fertilizer to the banana continuous cropping obstacle sick soil, and irrigating; covering the banana straw particles on the surface of the banana continuous cropping obstacle sick soil, then covering a layer of agricultural plastic film, fumigating, ploughing and planting peppers; pepper root systems and pepper straws are removed and smashed, and pepper straw particles are obtained; covering the pepper straw particles on the surface of the banana continuous cropping obstacle sick soil, then covering a layer of agricultural plastic film, fumigating and turning over. According to the conditioning method for the banana continuous cropping obstacle sick soil, the banana continuous cropping obstacle sick soil can be effectively conditioned, the morbidity of banana wilt and the morbidity of leaf spot diseases are reduced, the yield is increased, and economic benefits are effectively improved.

The invention provides a compound organic fertilizer as well as a preparation method and application thereof, and the compound organic fertilizer is prepared from the following raw materials in parts by weight: 40 to 50 parts of basic fertilizer, 5 to 10 parts of alkaloid compound preparation, 1 to 3 parts of dendrobium nobile stem extract, 2 to 4 parts of carnosic acid nutrient solution, 5 to 8 parts of compound fermentation liquor and 0.8 to 1.2 parts of antibacterial peptide, the alkaloid compound preparation is prepared from nicotine, caffeine, theobromine, sophocarpidine and colchicine according to the mass ratio of 1: (1 to 2): (0.8 to 1.2): (0.5 to 0.8): (1.2 to 1.5); the compound fermentation liquor is prepared from earthworm fermentation liquor and chitin fermentation liquor in a mass ratio of 1: (3-5). According to the compound organic fertilizer disclosed by the invention, the alkaloid compound preparation, the dendrobium stem extract, the carnosic acid nutrient solution, the compound fermentation liquor and the antibacterial peptide are mixed with the basic fertilizer, so that the compound organic fertilizer has a prevention and treatment effect on phomopsis areca leaf spot and banana wilt, the yield increasing effect of bananas is remarkable, and the compound organic fertilizer has relatively good popularization and application values.

The invention relates to the technical field of microbial control, and discloses bacillus velezensis E17-23 and application thereof. The bacillus velezensis E17-23 disclosed by the invention can be used for inhibiting citrus liberobacter asiaticum, citrus canker pathogen, citrus brown spot pathogen, citrus colletotrichum gloeosporioides, citrus lipolytic yellow spot pathogen, banana fusarium wilt pathogen and banana long leaf spot pathogen, and the inhibition effects are all greater than 60%. The inhibition effect of the sterile fermentation filtrate of the bacillus velezensis E17-23 on the pathogenic bacteria is more than 20.83%. The bacillus velezensis E17-23 can also promote plant growth, the average growth amount of treated orange seedlings is increased by 177.57% compared with that of a control group, and the number of new leaves is increased by 208.5% compared with that of the control group. The application of the bacillus velezensis E17-23 in the aspects of biological control and plant growth promotion has important significance in green agriculture and sustainable agricultural production.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

The present invention provides a Streptomyces, named: Streptomyces coffeae , preserved in Guangdong Province Microbial Culture Collection Center, with the preservation number of GDMCC No: 64532. The Streptomyces and its fermentation liquid of the present invention have good antibacterial properties against banana wilt No. 4 physiological subspecies, gloeosporium anthracnose, cucumber wilt, banana anthracnose, litchi anthracnose, mango anthracnose, strawberry anthracnose, banana leaf spot pathogen, rice blast pathogen, banana gray spot pathogen, mango leaf blight pathogen, etc., and the antibacterial rate reaches more than 50%. Moreover, the growth of banana seedlings can be effectively promoted under the attack of pathogens, and the product has resistance to multiple antibiotics. The product is a potential biological agent for preventing and treating anthracnose diseases, wilt diseases, etc. and promoting plant growth, etc., and has broad development space and good development and application prospects.

The pine wood nematode disease unmanned aerial vehicle monitoring and positioning device comprises an unmanned aerial vehicle body, a mounting block is mounted at the bottom of the unmanned aerial vehicle body, and an auxiliary flight stabilizing structure for improving flight stability by means of airflow is arranged in the mounting block; the bottom of the mounting block is provided with a plugging structure for cooperating with the auxiliary flight stabilization structure, and the bottom of the mounting block is connected with a box body in the middle of the plugging structure. According to the pine wood nematode disease unmanned aerial vehicle monitoring and positioning device and method, cooperative work of the flight stabilizing structure and the blocking and triggering structure is assisted, an airflow channel is optimized, the flight stability of the unmanned aerial vehicle under the conditions of complex terrains and severe weather is enhanced, energy consumption is reduced, and the endurance time is prolonged. In addition, the device is provided with a wireless communication module and a network adapter, and collected and processed data can be transmitted to a ground control station or a cloud server in real time.

The invention discloses a bacillus velezensis strain and application thereof. The invention discloses bacillus velezensis WLH (bacillus velezensis), and the preservation number of the bacillus velezensis WLH is CCTCC (China Center for Type Culture Collection) NO: M 20251238. The bacillus velezensis WLH is screened from rhizosphere of cucumber, the WLH is a biocontrol bacterium, can inhibit growth of pathogenic bacteria of cucumber root rot and fusarium wilt by degrading phenolic acid in soil and promote growth of cucumber plants, and the strain can relieve autotoxicity caused by the phenolic acid, inhibit the cucumber root rot and fusarium wilt and promote growth of cucumber plants. The promotion of crop growth comprises the capability of producing indoleacetic acid (IAA) and the capability of solubilizing phosphate, the promotion of germination of cucumber seeds under the stress of mixed phenolic acid and pathogenic bacteria, and the promotion of growth of cucumber seedlings under the stress of mixed phenolic acid and pathogenic bacteria. Soil continuous cropping obstacles generated in cucumber planting are effectively relieved, and a new basis is provided for microbial control of continuous cropping obstacles.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention discloses a multi-source data fusion early warning and precision application method and system for pine wilt disease, belonging to the field of smart forestry technology. It includes a multi-source data acquisition module, a spatiotemporal data fusion module, a health index calculation module, an external feature extraction module, a dual-channel coupled diagnosis module, a graded early warning module, an application urgency assessment module, an application strategy planning module, and an adaptive optimization module. This invention collects and fuses multi-source data such as satellite remote sensing data, UAV inspection data, ground-based IoT data, and forest meteorological data. It utilizes a dual-channel coupled deep learning model to achieve probabilistic diagnosis of the multidimensional state of individual trees and to extrapolate the spread of the disease. Based on the diagnostic results, it generates graded differentiated application strategies and finally achieves adaptive closed-loop optimization of the calculation parameters and model through a control effect feedback mechanism.

The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing nano-zinc oxide and brucea javanica extract and application of the bactericidal composition, and the mass ratio of the nano-zinc oxide to the brucea javanica extract in active ingredients of the bactericidal composition is 40: 1-1: 40; the bactericidal composition disclosed by the invention has an excellent bactericidal effect, and shows excellent control effects on watermelon fusarium wilt, wheat stem rot, tobacco bacterial wilt, cotton damping-off, cotton damping-off, peanut root rot, peanut stem rot, damping-off, fusarium wilt, damping-off, root rot and the like of fruits, vegetables and beans; the bactericidal composition enhances the bactericidal broad-spectrum property and efficiency, is environment-friendly, does not generate phytotoxicity, is safe to crops, and has a good application prospect.