650 results about "Wilt disease" patented technology

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The invention relates to a trichoderma harzianum composite bacteria culture and application of the same in the aspect of plant protection, and belongs to the technical field of biology. The strain of the trichoderma harzianum composite bacteria culture is preserved in the China General Microbiological Culture Collection Center (CGMCC), and classified and named as Trichoderma harzianum MDCGTH 18, with a preservation number of CGMCC NO.2965. The Trichoderma harzianum MDCGTH 18 composite bacteria culture utilizes antagonistic microbes for biological prevention and treatment, simultaneously has the functions of inducing the disease resistance of plants and increasing the yield, has obvious effect of controlling aspen root rot, apple root rot, citrus yellow shoot, watermelon fusarium wilt, tobacco bacterial wilt, wheat scab, full rot, root rot, banded sclerotial blight, cucumber powdery mildew, downy mildew, vegetable gray mold, fusarium wilt and various other epidemic diseases, can be independently taken as a biological control microbial inoculum for plant diseases, and can also be mixed with other biological antiseptics, plant antibiotics, plant growth regulators and the prior antiseptics to prepare various biological antiseptics for controlling different plant diseases.

The invention provides bacillus amyloliquefaciens K-8 and bactericide thereof. The preservation number of the bacillus amyloliquefaciens K-8 strain is CGMCCNO.6486. The bactericide takes the bacillus amyloliquefaciens K-8 strain as an active ingredient. The bacillus amyloliquefaciens K-8 strain is cultured to obtain a liquid bactericide, and a solid bactericide can be obtained by adding a carrier or mixture of the carrier and auxiliaries into the liquid bactericide. The bacillus amyloliquefaciens K-8 strain and the bactericide thereof provided by the invention can be used for effectively preventing and treating diseases such as bacterial wilt, wilt, angular spot, banded sclerotial blight, rice false smut, soft rot, rot, canker and root rot. The bactericide is simple to prepare and easy to use, is applicable to industrialized production and has wide application prospect.

The invention relates to bsacillus subtilis and microbial inoculum using the bsacillus subtilis as active ingredients, which are applied to the field of fruit freshness keeping. The bsacillus subtilis related by the invention is named as Bsacillus subtilis J18, which is preserved in China General Microbiological Culture Preservation and Management Center at present with the preservation number of CGMCC NO: 3665. Strains are prepared into fermentation agents after fermentation culture, and the fermentation agents are respectively mixed with calcium chloride and chitosan solution to be prepared into dry powder preparations and fruit glaze agents with obvious prevention and treatment effects on pear melasma after picking, black rot of pear, grape grey mould, watermelon wilt and the like. The bsacillus subtilis J18 belongs to a strain of antagonistic bacteria with wide-spectrum resistance on fruit and crop diseases.

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention discloses a composite bio-formulation for preventing crop diseases, comprising the following raw materials: trichoderma pseudokoningii fermenting liquid, bacillus subtilis fermenting liquid, water-soluble chitosan, sodium glutamate residue liquid and potassium humate, wherein the trichoderma pseudokoningii and the bacillus subtilis are high-effect strains which are obtained after being screened and processed, later the fermenting liquid of the trichoderma pseudokoningii and the bacillus subtilis is obtained through a submerged fermentation technique, and the composite bio-formulation for preventing crop diseases is obtained through combining. The composite bio-formulation for preventing crop diseases in the invention has obvious effects for preventing blight, verticillium, gray mold, phytoph-thora capsici leonian, downy mildew, powdery mildew, gray mold, fulvia fulva and so on, and can be especially used for preventing the blight and the verticillium which have large danger in agriculture. The invention is suitable for preventing the diseases and improving yields of various vegetables, melons, fruits and crops, such as tomatoes, chili peppers, eggplants, cucumbers, watermelons, apples, Chinese chives, cottons and so on.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

The invention relates to a trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and a preparation method thereofof the trichoderma atroviride preparation. A trichoderma strain Tr1208 is trichoderma atroviride and preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No. 6828. According to The the trichoderma atroviride preparation takes, the trichoderma atroviride Tr1208 is used as an effective active ingredient and is prepared by being matched with an agriculturally acceptable carrier and a protective agent for the preparation; the bacteria content of the effective active ingredient is (1.0-30.0)*108 spores / gram; and the bacteria protectant content is 0.05%-1.8%. The preparation can have the effects of effectively preventing and controlling leek Botrytis, cucumber brown patches, cucumber powdery mildew, cucumber downy mildew, tomato early blight, cucumber type damping off, cucumber type blight and cucumber type root rot and promoting plant growth and characteristics of efficiency, wide antimicrobial spectrum, simple usage, low cost, no environmental pollution and plant micro ecological environment improvement.

The invention discloses a method for building a mountainous area surface temperature base line and application, and relates to the measurement and application of mountainous area surface temperature base lines. A transient temperature field distributed continuously in MODIS thermal infrared remote sensing image inversion spaces is combined with longitude and latitude raster data to build the annual average temperature linear estimation model of a weather station as the calibration model of the transient surface temperature field. The model corrects the transient temperature field to obtain the mountainous area surface temperature base line. In YunnanProvince with the mountain landform as the main landform, the temperature base line with a 90m*90m grid as a unit is built, the temperature distribution characteristics and rules of the area are reflected, and the average temperature base line value of a 90m*90m land parcel can be found out in a quantitative mode. The temperature base line is used as basic data to be combined with an expert scoring method to respectively build a Yunnan Province pine wilt disease occurrence risk pre-warning model and a Yunnan Province forest fire danger long-term forecast model.

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention discloses a trichoderma spore powder as well as a preparation method and application thereof. The trichoderma spore powder contains trichoderma spore powder and a drying aid, wherein the trichoderma spore powder is the spore powder of Trichoderma sperellum T1 which is preserved in the general microorganism center of CCCCM (China Committee for Culture Collection of Microorganisms) and has the preservation number of CGMCC NO: 3531 and a rDNA ITS region segment sequence shown in SEQ ID NO:1; the drying aid is one or more of beta-cyclodextrin, potato starch and soluble starch. The trichoderma spore powder has an effective prevention and control effect on banana vasicular wilt, and has a remarkable antagonism effect on various pathogenic funguses, especially on soil-borne funguses. The preparation time is saved with the preparation method of the trichoderma spore powder, which can be used for preparing the trichoderma spore powder which has high density, high germination rateand high drying efficiency and is easy to store, transport and use.

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention relates to an emamectin benzoate injection to prevent pine wilt disease, which is characterized in that, the components of the invention are 0.5% to 2% emamectin benzoate, 10% to 15% tween 80 or tween 60, 15% to 20% butyl glycol ether and 65% to 72% organic solvent; the components are arranged in an injection container; the percentage is mass percentage. The emamectin benzoate injection has the advantages that, compatibility composition is more reasonable and more pertinent; through proper surface-active material and addition agent, the conduction of emamectin benzoate in pines is easier, therefore pine wood nematode can be eliminated; the form of the pharmaceutical preparation is more reasonable and more scientific; the invention has convenient use and practicality; active substance is added; combined with injection, the invention has better killing effect.

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technical field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The biological and biochemical preparation is a kind of multiple target site preparation containing Trichoderma pseudokiningii and Bacillus spp as well as cell membrane protecting matter, composite inductor, natural antibiotic matter, such as trace elements, and beneficial matters, such as taurine, vitamin C, etc. Trichoderma pseudokiningii is strain obtained through separating and screening stalk and named as TRIP1, and Bacillus spp is separated from soil and named as BAC1. The biological and biochemical preparation may be used in preventing and controlling several kinds of plant diseases, promoting growth of plant, protecting cell membrane and raising plant photochemical efficiency.

The invention provides a Monochamus alternatus adult attractant. The Monochamus alternatus adult attractant comprises an artificially-synthesized compound, and the compound is prepared through adding reagents comprising undecyl alcohol, thionyl chloride, glycol, metallic sodium and the like. The Monochamus alternatus adult attractant comprises the artificially-synthesized compound, so the effective application amount is less; the Monochamus alternatus adult attractant has a high trapping effect on Monochamus alternatus, and can be used as an effective means for monitoring and controlling the Monochamus alternatus to inhibit and delay the propagation of the pine wilt disease; and the Monochamus alternatus adult attractant generally has the advantages of simple components, low production cost, safety, environmental protection, high insect trapping efficiency, and advantage obviousness.

The invention provides a nutrient fertilizer for fuchsia. The nutrient fertilizer can meet the basic nutrient requirements of the whole growth period of the fuchsia, and is favorable for promoting growth and development of roots and leaves of the fuchsia. The fertilizer has sterilization, pest killing and pest and disease prevention while being applied, and has a good effect of preventing, controlling or relieving aphids, blight, rust disease, root rot, powdery mildew and the like possibly produced in the fuchsia. After the fertilizer is applied, the fuchsia has the advantages of large and multiple flower buds, bright color, few pest and disease and quick growth and development speed and also has high ornamental value.

The invention discloses an amino acid compound fertilizer, a preparation method and an agriculture ecological cycle development method. The amino acid compound fertilizer is prepared from the following raw materials: amino acid solution, copper sulfate, zinc sulfate, magnesium sulfate, ferrous sulfate, manganese sulfate, ammonium molybdate, sodium selenite, borax, sodium nitrophenolate, citrate, metasilicate or silicate, and urea. According to the amino acid compound fertilizer, the amino acid or micromolecular active protein in the amino acid solution are chelate with various essential elements for plant, cotton wilt disease can be effectively prevented and controlled, the restoration of crops which suffer from chilling damage or hails can be promoted, and the crops with toxicity due to excessively applied elements and herbicide toxicity can be effectively detoxified; functionalized health-care crop products rich in selenium, silicon, glutathione peroxidase, superoxide dismutase, peroxidase and catalase can be harvested.

The invention relates to a bactericidal composition containing prothioconazole, which contains active ingredient A and active ingredient B, wherein active ingredient A is selected from prothioconazole, and active ingredient B is selected from thiophanate-methyl, multibacterium One of Ling and prochloraz, wherein the weight ratio of active ingredient A to active ingredient B is 1:80 to 80:1, made into wettable powder, water dispersible granule, suspending agent, suspoemulsion, water emulsion, Microemulsion, used to control powdery mildew, sheath blight, fusarium wilt, leaf spot, rust, sclerotinia, viral disease, anthracnose, gray mold, ring spot, bakanae disease, rice blast, early blight, Diseases such as green mold, pedicle rot, penicillium, flax spot, scab, blight, etc. have the characteristics of small dosage, resistance to rain erosion, and obvious synergistic effects.

The present invention relates to bacillus amyloliquefaciens and a preparation method of a microbial inoculum of the bacillus amyloliquefaciens, and belongs to the field of bio-pesticides and plant protection. The bacillus amyloliquefaciens HN-11 is isolated from azadirachta indica tree rhizosphere soil, azadirachta indica residue is used as a medium raw material and a microbial inoculum adsorption carrier for secondary solid state fermentation composting to prepare the microbial inoculums, the microbial inoculums comprises an arbitrary content of the bacillus amyloliquefaciens, 0 to 30% of the azadirachta indica residue, 4 to 12% of the soybean powder, 5 to 25% of wheat bran, 30-50% of peat, 1-5% of KCl, 0.5 to 2% of urea and 1 to 8% of a surfactant. Tests show that the microbial inoculum can overcome the problem of seedling injury caused by direct use of the azadirachta indica residue, can expand the control spectrum, has obvious integral control effect on banana wilt and Radopholus similes Thorne, can also promote plant growth, and the field control effect on the banana wilt can reach 96.2%. The microbial inoculum has great significance to the promotion of integral pest control and agricultural and sideline product recycling, and has broad application prospects in the production practice.

The invention discloses a monochamus alternatus hope attractant and an application thereof. The monochamus alternatus hope attractant comprises a plant source attractant and aggregation pheromone 2-undecyl-1-ethanol, wherein the plant source attractant comprises the following components in percentage by volume: 60-85% of alpha-pinene, 0-20% of beta-pinene and 15-40% of ethanol; the dosage of the aggregation pheromone 2-undecyl-1-ethanol in each lure is 0.5-100mg. The novel compound attractant has the advantages of simple production process, low cost, ideal trapping effect, safety, environment friendliness and the like, and can be used for effectively controlling the inter-forest insect density of monochamus alternatus hopes and inhibiting the spread and propagation of pine wilt disease.

The invention discloses an adult monochamus alternatus hope attractant, which belongs to the technical field of prevention and control of pests. The attractant is characterized by comprising 95 percent alpha-pinene and 40 percent aldehyde in the volume ratio of (1-5):1. In the adult monochamus alternatus hope attractant, the 95 percent alpha-pinene serving as an active ingredient and the 40 percent aldehyde serving as a synergistic substance are compounded, so that the attracting effect of the attractant is enhanced greatly and the trapping quantity of adult monochamus alternatus hopes in a feeding period accounts for 70 percent of the total trapping quantity; moreover, the attractant has the advantages of simple production process, low cost, environmental friendliness and the like, is safe, can effectively control the woodland population density of monochamus alternatus hopes and prevents the spreading and the transmission of pine wilt disease.

The invention discloses a strain of Paenibacillus jejuni for inhibiting anthrax and Fusarium and an application thereof. A strain of Paenibacillus jamilae is isolated from the deep sea sediment, the strain was deposited at the General Microbial Center of the China Microbial Culture Collection Management Committee under accession number CGMCC No.16231 on August 8, 2018. The strain has broad-spectrum resistance to many plant pathogens, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by Colletotrichum gloeosporioides and vegetable wilt caused by Fusarium oxysporum.

The invention relates to a biocontrol bacterial agent. An effective active constituent consists of bacillus subtilis PTS-394. The biocontrol bacterial agent is characterized in that: the biocontrol bacterial agent is aqueous solution or wettable powder. The aqueous solution comprises the following components in percentage by weight: bacillus subtilis PTS-394 with 2 *1,010 cfu / mL of content of bacteria, 0.5 percent of NNO (sodium methylene naphthalene sulfonate), 6 percent of SDBS (sodium dodecyl benzene sulfonate) and 0.1 percent of sodium benzoate. The wettable powder comprises the following components in percentage by weight: 40 percent of bacillus subtilis PTS-394 viable bacterium raw powder with 5 *1,010 cfu / g of content of bacteria, 7 percent of NNO (sodium methylene naphthalene sulfonate), 3 percent of polyethylene glycol, 5 percent of SDBS (sodium dodecyl benzene sulfonate) and 45 percent of light calcium carbonate. The biocontrol bacterial agent is poured into vegetable roots when applied and is used for preventing and controlling vegetable soil-borne diseases such as solanaceous fruit vegetable blight and bacterial wilt mainly; and the content of effective agent components is between 104 and 108 cfu / mL or between 104 and 108 cfu / g after finished product agents are diluted. The biocontrol bacterial agent has the advantages that: the aims of preventing and controlling two kinds of soil-borne diseases by means of one kind of biocontrol bacterial agent are fulfilled by introducing the bacillus subtilis PTS-394 which is safe to people and livestock and has better inhibition effect on the solanaceous fruit vegetable blight and the bacterial wilt; the using amount of chemical agents is reduced, and the agent cost is reduced; and the ecological environment is improved, and the residues of the chemical agents in the vegetables are reduced and the same time.

The invention discloses a bactericidal composition containing metconazole and an antibiotic compound. The bactericidal composition contains an active ingredient A and an active ingredient B, wherein the active ingredient A is metconazole, the active ingredient B is selected from any one of the following bactericides: validamycin, polyoxin and kasugamycin, and the weigh part ratio of the active ingredient A to the active ingredient B is (1-64): (64-1). The composition can control various crop diseases, has remarkable synergistic action, enlarges the bactericidal spectrum, has higher activity to rice blast, powdery mildew, downy mildew, sheath blight, botrytis, leaf mold, fusarium wilt, plague and alternaria mali; in addition, agricultural chemical dosage, the residue of agricultural chemical on crops and environment pollution are reduced.

The invention discloses paenibacillus polymyxa JX-13 of which the classification is named as paenibacillus polymyxa. The paenibacillus polymyxa JX-13 is preserved in the common microorganism center of the Chinese microorganism culture preservation management committee, the preservation number is CGMCC No.9796, and the preservation date is October 17, 2014. The invention further discloses the application of the paenibacillus polymyxa JX-13. According to the paenibacillus polymyxa JX-13, wettable powder prepared by the fermentation of the strain can effectively prevent and control diseases such as strawberry blight, anthracnose and grey mold and grape grey mold and anthracnose. The storing property of the bacterium agent is stable, the control effect is good, and the mass production fermentation technology is simple and low in production cost.

The invention provides Bacillus siamensis and application thereof, and belongs to the technical field of microbial prevention and control. Research shows that Bacillus siamensis LZ88 provided by the invention has very good effects on the biological control of plant diseases such as tobacco brown spot, tobacco black shank, wheat scab, grape anthracnose, cucumber fusarium wilt, peanut fusarium and the like, the induction of the resistance to tobacco plant diseases and the like, and provides a new strain resource for biological prevention and control of main diseases of tobacco, such as brown spot, and the like, thereby having good practical application value.

The invention relates to a composite biological antimicrobial for preventing wilt and greensickness of cotton. The active components of the composite biological antimicrobial comprise Candida tropicalis, streptomyces microflavus, Trichoderma viride and Bacillus subtilis. The composite biological antimicrobial for preventing wilt and greensickness of cotton can reduce the seedling death rate effectively, has obvious preventing and treating effect on wild and greensickness of cotton and provides the cotton output.