124 results about "Wilt disease" patented technology

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention discloses a multi-source data fusion early warning and precision application method and system for pine wilt disease, belonging to the field of smart forestry technology. It includes a multi-source data acquisition module, a spatiotemporal data fusion module, a health index calculation module, an external feature extraction module, a dual-channel coupled diagnosis module, a graded early warning module, an application urgency assessment module, an application strategy planning module, and an adaptive optimization module. This invention collects and fuses multi-source data such as satellite remote sensing data, UAV inspection data, ground-based IoT data, and forest meteorological data. It utilizes a dual-channel coupled deep learning model to achieve probabilistic diagnosis of the multidimensional state of individual trees and to extrapolate the spread of the disease. Based on the diagnostic results, it generates graded differentiated application strategies and finally achieves adaptive closed-loop optimization of the calculation parameters and model through a control effect feedback mechanism.

This invention provides compounds and methods of application that possess both broad-spectrum antifungal activity and plant-induced resistance, belonging to the field of agricultural technology. It addresses the problem that "traditional pure immune inducers must be applied before disease onset, making it difficult to meet the full-cycle control needs of disease prevention and treatment in the field." Compared to the previously developed PC1 and PC20, the two compounds of this invention, 2,4-dibromo-6-nitrophenol and 7-deazon-2'-deoxy-7-iodoadenosine, can both inhibit the growth of rice blast fungus and significantly improve rice resistance to rice blast through foliar spraying, meeting the full-cycle control needs of disease prevention and treatment in the field. Furthermore, these two compounds can directly inhibit the growth of various fungal pathogens such as peanut white mold, tomato gray mold, wheat scab, rice bakanae disease, rice false smut, mango malformation, and banana wilt.

The application provides application of a bacillus subtilis K8 nano-seleniumized active bacterium agent in prevention and treatment of plant pathogenic bacteria, and belongs to the technical field of biological prevention and treatment of plant diseases. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent is obtained by fermenting and culturing bacillus subtilis K8 in a fermentation medium containing sodium selenite. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent has a strong prevention and treatment effect on cucumber fusarium wilt, cucumber botrytis blight, tomato early blight and tomato root rot, provides an efficient, safe and practical method for prevention and treatment of plant fungal diseases, has low cost, simple preparation process, is convenient for industrialization, and has good development value and market prospect.

The invention belongs to the technical field of molecular biology, and discloses a molecular marker remarkably related to cassava bacterial wilt resistance and application of the molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to cassava bacterial wilt resistance, which is characterized in that the sequence of the SNP molecular marker is shown as SEQ ID NO: 3, the SNP site is positioned at the 392nd site from the 5'terminal of the sequence shown as SEQ ID NO: 3, and the polymorphism of the SNP molecular marker is A / T. By detecting the genotype of the SNP molecular marker, cassava with strong resistance to bacterial wilt can be accurately selected, and the SNP molecular marker can be effectively used for cassava molecular marker assisted breeding; according to actual breeding requirements, genotype identification can be carried out on cassava, genotype cassava with high bacterial wilt resistance is selected for hybridization, cassava filial generation with high bacterial wilt resistance is obtained, breeding time is saved, cost is low, accuracy is high, and the cassava breeding process is accelerated.

The application provides bacillus velezensis LB17, K87, MP6 and a compound microbial inoculant thereof, a preparation method and application, and belongs to the technical field of biocontrol bacteria.The application collects rhizosphere and rhizosphere soil of characteristic crops such as highland barley, lily, tomato and pepper in the Gansu-Qinghai region, and obtains 305 pure cultures through separation, obtains three strains of bacillus velezensis LB17, K87 and MP6 through multiple screening, and finally obtains a compound fermentation microbial inoculant, i.e., tuoweiwei No.7, which is fermented by the three strains of bacillus velezensis LB17, K87 and MP6 through optimization of inoculation amount, components of culture medium, ratio and fermentation conditions.Tuoweiwei No.7 can effectively prevent plant diseases such as pathogenic microorganisms, root rot and fusarium wilt, and can promote plant growth and improve the stress resistance of plants.The compound microbial inoculant provided by the application provides a strong guarantee for the prevention and control of root rot diseases of special habitat crops in the Gansu-Qinghai region.

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The invention relates to the field of plant genetic engineering, and discloses a corn sheath blight disease-resistant gene GRMZM2G057329 and application thereof, and the corn sheath blight disease-resistant gene GRMZM2G057329 comprises any one of the following sequences: a nucleotide sequence shown as SEQ ID No.1, a nucleotide sequence shown as SEQ ID No.2, a nucleotide sequence shown as SEQ ID No.3, a nucleotide sequence shown as SEQ ID No.4, a nucleotide sequence shown as SEQ ID No.5, a nucleotide sequence shown as SEQ ID No.6, a The nucleotide sequence is as shown in SEQ ID No.2; the amino acid sequence of the protein is shown as SEQ ID No.3; the nucleotide sequence has 90% or more of identity with the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 and is used for coding protein with the same function as the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2. According to the present invention, the DNA fragment of the complete coding segment of the anti-disease gene GRMZM2G057329 separated and cloned from corn is adopted, and the GRMZM2G057329 gene expression quantity is increased so as to significantly enhance the sheath blight resistance of the corn genetic transformation plant;

The application discloses a kind of low temperature resistant strain YW-1 for preventing and treating rice seedling wilt, and belongs to the field of plant protection.The low temperature resistant strain YW-1 is a pseudo-odor class fungus, and the application proves that the strain YW-1 can maintain normal growth and metabolism in a low temperature environment of 15 DEG C, and can effectively destroy the hyphal structure of the wilt pathogen by secreting cellulase and chitinase, thereby inhibiting the occurrence of the disease from the source.The strain YW-1 has no antagonism with the commonly used functional fungus Trichoderma in the seedling raising substrate, and the two can be compounded and coexist, thereby realizing the synergistic effect of functional microorganisms.The application overcomes the defects of the existing biocontrol strain, such as reduced activity in a low temperature environment and unstable prevention effect, and provides a stable and reliable biological control scheme for factory seedling raising in a low temperature season without increasing the amount of chemical pesticides, thereby significantly improving the quality of seedlings and the seedling raising success rate.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The invention relates to the technical field of pine wood nematode disease detection, in particular to an unmanned aerial vehicle remote sensing image pine wood nematode disease detection method based on SLMW-Net. According to the method, a pine wood nematode disease ARen data set is used for model training and testing, meanwhile, a self-learning feature extraction block is introduced and can effectively extract pine wood features infected with nematode diseases in a scene with complex surface conditions and remarkable vegetation interference, a slight feature attention mechanism is provided, and the method has the advantages of being high in accuracy and high in accuracy. The mechanism effectively enhances the capability of capturing slight details of pine wood nematode characteristics, keeps the attention on global characteristics, improves the accuracy of characteristic expression, and overcomes the limitation of a traditional attention mechanism in capturing slight disease characteristics, so that the accuracy of pine wood nematode detection is remarkably improved, and in addition, the mechanism can be applied to the field of detection of pine wood nematode. A WLIOU Loss loss function is designed to optimize a training process, and the problems of class imbalance and difficulty in sample detection are effectively solved.

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

A pine wilt disease tree detection method based on an attention mechanism and a center circle sampling method, which comprises the following steps: step 1, obtaining pine wilt disease tree pictures and making them into a data set; step 2, inputting the pine wilt disease tree data set into a global multi-scale channel adaptive network for training to obtain a trained pine wilt disease tree recognition model; step 3, inputting pine forest images that need to be recognized into the pine wilt disease tree recognition model to obtain the recognition result of the diseased tree and the latitude and longitude coordinates; and step 4, finding the diseased tree according to the coordinates of the diseased tree, and performing drug treatment on the early-stage diseased tree and cutting and burning treatment on the middle- and late-stage diseased tree. The purpose of the present application is to solve the technical problem that the existing pine wilt disease tree detection method ignores the mismatch between the positive sample sampling range and the circular shape of the pine wilt disease tree target, resulting in low sampling quality.

This invention discloses a banana-specific alkaline biocontrol organic fertilizer and its preparation method, belonging to the field of bio-organic fertilizer technology. The banana-specific alkaline biocontrol organic fertilizer of this invention comprises the following raw materials: 25-30 parts modified tobacco straw carbonized powder, 20-25 parts moringa seed fermentation product, 5-10 parts cactus fermentation product, 10-14 parts seaweed residue, 8-12 parts humic acid powder, and 3-5 parts shell powder. The moringa seeds are fermented using enzymatic hydrolysis and microbial agents, effectively breaking down large molecules into smaller molecules that can be directly absorbed by banana plants, promoting plant growth. Simultaneously, *Achromobacterium solani* and *Acinetobacter rhizogenes* secrete various active substances, significantly enhancing banana disease resistance and growth promotion. This invention's organic fertilizer, with its synergistic effect of multiple substances, reduces the use of chemical fungicides, effectively inhibits banana wilt disease, neutralizes soil acidity, increases banana yield, and improves economic and social benefits.

The invention belongs to the field of microorganisms, and provides bacillus velezensis NH1 / 8 which is preserved in the China General Microbiological Culture Collection Center on July 23, 2025, the preservation number is CGMCC No.35343, the address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the postal code is 100101. The NH1 / 8 strain disclosed by the invention shows a remarkable antibacterial effect on three pathogenic fungi (tobacco brown spot, tobacco fusarium wilt and tobacco black shank), and shows that the NH1 / 8 strain has an antagonistic effect on the pathogenic fungi.

The invention discloses bacillus velezensis GJ5B6 and application thereof, and belongs to the technical field of biocontrol microorganisms, the preservation number of the bacillus velezensis GJ5B6 is CCTCC NO: M 20252105, and the bacillus velezensis GJ5B6 is preserved in China Center for Type Culture Collection on September 24, 2025. The bacillus velezensis GJ5B6 has remarkable inhibitory activity on pathogenic bacteria of the waxberry wilt disease and has a wide application prospect in the aspect of prevention and treatment of the waxberry wilt disease, and a theoretical basis and a strain resource are provided for development of environment-friendly biocontrol preparations; the biological agent prepared by taking the bacillus velezensis GJ5B6 as an active ingredient does not cause the problems of resistance of pathogenic bacteria, environmental pollution, pesticide residues and the like after being used for a long time, and is more environment-friendly and safer than a chemical control method.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

This invention discloses a biocontrol agent for controlling sesame wilt, belonging to the field of pesticide technology. The raw materials of this biocontrol agent for controlling sesame wilt, by weight, include: 8-15 parts of a pyrimidine nucleoside antibiotic, 0.5-5 parts of matrine, 0.5-2 parts of an antioxidant, 1-5 parts of a dispersant, 1-3 parts of an adhesive, and 2000 parts of water. This invention utilizes a mixture of the pyrimidine nucleoside antibiotic and matrine to achieve a synergistic effect. The mixed product system allows matrine to enter the plant more easily, further improving its rapid action and systemic absorption. Furthermore, by simultaneously utilizing the different bactericidal mechanisms of the pyrimidine nucleoside antibiotic and matrine, the problem of the slow onset of action of the pyrimidine nucleoside antibiotic is addressed, thus enabling faster and more effective control of sesame wilt.

The application relates to the technical field of plant grafting, and particularly discloses a grafting method capable of improving disease resistance of eggplants, which comprises the following steps: Solanum nigrum seedling, eggplant seedling, seedling period management and grafting; in the application, the wild Solanum nigrum is selected as the stock, has the advantages of fast growth speed and good disease resistance, and can greatly improve the disease resistance and yield of the plant as the stock; the eggplant has a high incidence rate during the growth process, especially the occurrence of the yellow wilt disease is serious, and the yield is completely lost in serious cases; the grafting can solve the fundamental problem; the Solanum nigrum seed has a large amount, is easy to breed, and has a high germination rate; the special nutrient solution is sprayed, the wound healing speed of the grafting is greatly accelerated, and the healing effect is good; the grafting method of the sleeve pipe is adopted, the grafting speed is improved, the wound is protected, and the bacteria are prevented from invading the wound.

This invention discloses a compound fungal agent containing Penicillium citrinum and Trichoderma guizhouense, wherein the Penicillium citrinum is Penicillium citrinum (… Penicillium citrinum NJAU-T106, the Guizhou Trichoderma is Guizhou Trichoderma ( Trichoderma guizhouense NJAU4742. This invention also discloses its preparation method and its application in promoting banana plant growth, increasing banana yield, and controlling banana wilt disease. This invention screened Penicillium citrinum (NJAU4742) from banana disease-suppressing soil to obtain a strain with good biocontrol and growth-promoting effects. Penicillium citrinum The study used NJAU-T106 and innovatively combined it with Trichoderma guiyuanensis NJAU4742 to construct a synergistic system of Penicillium citrinum and Trichoderma guiyuanensis. This effectively reduced the incidence of banana wilt disease while better promoting banana plant growth and increasing banana yield, thereby driving the green and sustainable development of the banana industry.

The present application relates to a kind of containing piperazine derivative structure and its application.The compound has the structure as one of general formula (I-III) shown: The compound is as rice bacterial leaf blight, tobacco bacterial wilt, cucumber bacterial leaf blight, konjac bacterial leaf blight, citrus bacterial wilt, grape bacterial wilt, tomato bacterial wilt, kiwi bacterial wilt, apple bacterial wilt, cucumber bacterial wilt, pepper wilt pathogen, sclerotinia sclerotiorum, fusarium graminearum, potato late blight pathogen, blueberry root rot pathogen and the like has good inhibitory effect.

The invention provides a waxberry branch and leaf wilt disease control method and a liquid medicine spraying device thereof. The waxberry branch and leaf wilt disease prevention and control method comprises the following steps: A, laying a running track; b, AGV cruise sampling is carried out; c, processing and analyzing; and D, processing. The liquid medicine spraying device comprises a main pipe, branch pipes and sprayers, the main pipe is provided with a plurality of pipe sections adjacent front and back in the length direction, the branch pipes are arranged on the two sides of each pipe section, the sprayers are arranged on the two sides of each branch pipe in the length direction, and the lower end of the main pipe is fixedly connected to the AGV and used for being connected with a liquid medicine pump. The outer diameters of the adjacent pipe sections of the main pipe are gradually increased from bottom to top, the outer ends of all the branch pipes serve as track points, the connecting lines of all the track points form a circular range ring, and the multiple nozzles are evenly distributed in the range ring. According to the method, the wilt disease of branches and leaves can be found and treated in time. The liquid medicine spraying device can spray liquid medicine to waxberries in a targeted mode.

The application discloses a composition for preventing and treating Bursaphelenchus xylophilus and application thereof, and belongs to the technical field of biological pesticides. The composition is compounded by taking a crude extract of Zanthoxylum bungeanum as a main active ingredient and a crude extract of Astragalus membranaceus as a synergistic ingredient. The composition has high Bursaphelenchus xylophilus killing activity. After Bursaphelenchus xylophilus is treated by 600ug / mL of the composition solution for 24 hours, the corrected mortality of the Bursaphelenchus xylophilus reaches 99.33%. The composition can effectively prevent the occurrence of the pine wilt disease in a forest, and the control effect reaches 79.59%. In addition, the preparation process of the composition is simple, the composition is easy to use, and the composition is suitable for popularization and use.

The invention belongs to the technical field of agricultural microorganisms, and relates to a biocontrol fungus for preventing and treating pomegranate fusarium wilt, a biocontrol microbial inoculum and application of the biocontrol fungus and the biocontrol microbial inoculum. According to the invention, a chaetomium globosum N9 strain is separated and screened from microbial resources on pomegranate branches, the chaetomium globosum N9 strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 67114. The N9 strain provided by the invention has a good antagonistic effect on the main pathogenic bacterium-sweet potato long-beak-shell fungus causing pomegranate fusarium wilt, and can significantly inhibit the growth of sweet potato long-beak-shell fungus hyphae, and the inhibition rate is up to 75.9%. The biocontrol fungus and the biocontrol inoculant provided by the invention have application prospects in the aspect of preventing and treating plant diseases caused by sweet potato coracocystis longirostris, especially in the aspect of preventing and treating pomegranate fusarium wilt.

This invention belongs to the field of fermentation engineering and plant disease control technology, specifically relating to the application of a Bacillus endophyticus compound inoculant fermentation broth in the control of plant diseases. The Bacillus endophyticus compound inoculant disclosed in this invention exhibits antibacterial effects against anthracnose in peppers, blight in peppers, gray mold in tomatoes, wilt in watermelons, wilt in cucumbers, verticillium wilt in cotton, black spot in peppers, root rot in carrots, soft rot in taro, and bacterial wilt in peppers, with inhibition rates of (75.37±1.44), (71.00±1.41)%, (83.14±0.96)%, (74.70±1.09)%, (69.21±1.04)%, (78.31±1.39)%, (73.06±0.73)%, (75.49±0.89)%, (46.79±1.57)%, and (73.82±0.51)%, respectively.

This invention discloses a fungal encapsulation agent for *Ischemicula elais* and its application in the control of pine wilt disease, belonging to the field of microbial control technology. This invention uses *Ischemicula elais* as the functional microorganism, combined with carrier materials, additives, and active ingredients to prepare a solid microbial encapsulation agent. The shelf life of *Ischemicula elais* spores at room temperature is extended from 2-3 days for traditional spore solutions to over 3-6 months, and up to 12 months under 4°C refrigeration, solving the problem of inconvenient low-temperature storage in mountainous areas. The microencapsulation components form a slow-release protective layer, preventing activity loss caused by environmental factors such as high temperature and drought.