The invention relates to a bactericidal composition containing prothioconazole, which contains active ingredient A and active ingredient B, wherein active ingredient A is selected from prothioconazole, and active ingredient B is selected from thiophanate-methyl, multibacterium One of Ling and prochloraz, wherein the weight ratio of active ingredient A to active ingredient B is 1:80 to 80:1, made into wettable powder, water dispersible granule, suspending agent, suspoemulsion, water emulsion, Microemulsion, used to control powdery mildew, sheath blight, fusarium wilt, leaf spot, rust, sclerotinia, viral disease, anthracnose, gray mold, ring spot, bakanae disease, rice blast, early blight, Diseases such as green mold, pedicle rot, penicillium, flax spot, scab, blight, etc. have the characteristics of small dosage, resistance to rain erosion, and obvious synergistic effects.