230 results about "Wilt disease" patented technology

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

The invention discloses a bacillus AH83 capable of resisting saline and alkaline and promoting growth and application thereof, and belongs to the technical field of microbiology and biology, the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495, and the bacillus AH83 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC No: M 20241495. The AH83 provided by the invention is a new species of bacillus, can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the AH83 can survive in a highly saline-alkaline environment, can promote agronomic characters such as plant height, biomass, leaf area and root development in a corn seedling stage when applied in saline-alkaline soil, can also increase soil nutrients and enzyme activity content, and has a promoting effect on lavender seed germination. The strain is of great significance in improving crop yield and stress resistance and developing novel agricultural microbial agents.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention relates to the technical field of biology, in particular to application of PnPUB35 protein and a coding gene thereof to regulation and control of plant wilt resistance. The invention provides a Ubiquitin ligase PnPUB35 protein containing a U-box structural domain E3 and a gene PnPUB35 of the Ubiquitin ligase PnPUB35 related to resistance to fusarium wilt of pepper, and after the pepper is inoculated with fusarium solani, the expression quantity of the Ubiquitin ligase PnPUB35 protein at the root of the pepper is increased. The resistance of a pepper PnPUB35 silent plant to fusarium solani is weakened, and the resistance of an arabidopsis thaliana PnPUB35 overexpression plant to fusarium solani is enhanced. The PnPUB35 gene disclosed by the invention plays a positive regulation role in pepper wilt resistance, and can be used as an important candidate gene for pepper wilt resistance breeding.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

Bacteriophages that comprise a tail fiber protein sequence comprising a C terminus host recognition site described by SEQ ID NO: 1 and which infect Ralstonia solanacearum which causes bacterial wilt disease are disclosed. Compositions comprising these bacteriophages may be employed as wilt control agents. Methods for preventing or treating diseases caused by Ralstonia solanacearum such as bacterial wilt disease are also disclosed. A bacterial wilt disease control method in a plant may include administering such bacteriophages to a plant or to a plant growth medium and can be effective against a wide variety of Ralstonia solanacearum strains. Nucleic acids encoding the C terminus host recognition site and amino acid sequences comprising the host recognition site are also disclosed.

The invention provides a pine wood nematode disease tree detection method and system based on image analysis, and relates to the technical field of image processing. The method comprises the following steps: acquiring a to-be-analyzed pine image acquired by an unmanned aerial vehicle, and determining a plurality of reference crown areas in the to-be-analyzed pine image; respectively carrying out diseased tree fading analysis and crown form analysis on each reference crown area, carrying out primary anomaly detection on the plurality of reference crown areas, and dividing to obtain a reference sample group and an abnormal sample group; performing clustering analysis based on environment distribution difference on the plurality of reference tree crown areas in the reference sample group to generate a plurality of environment influence tree crown groups; and determining an environment influence crown group to which each reference crown region belongs in the abnormal sample group, and carrying out secondary anomaly detection on the plurality of reference crown regions to obtain a pine wood nematode pest detection result of the to-be-analyzed pine image. According to the invention, precise detection of the pine wood nematode disease tree is realized.

The invention discloses a nano mimetic enzyme as well as a preparation method and application thereof. The nano-mimic is of a nano-cloud structure formed by stacking irregular spheres and rods, and the nano-cloud structure is a one-dimensional, two-dimensional or three-dimensional system. The preparation method of the nano mimic enzyme comprises the following steps: 1) dissolving a copper salt and a manganese salt in deionized water to obtain a mixed solution; 2) adding hydrogen peroxide into the mixed solution to obtain a Cu < 2 + > / Mn < 3 + > mixed solution A; 3) adding the alkaline solution B into the Cu < 2 + > / Mn < 3 + > mixed solution A, stirring and mixing to obtain a mixed brown turbid solution, and standing and aging; and (4) precipitating and separating the aged turbid liquid in the step (3), cleaning, drying and grinding to obtain the nano mimic enzyme. The nano mimetic enzyme suspension is applied to watermelon seed priming. The nano enzyme mimetic suspension disclosed by the invention is applied to preparation of antibacterial drugs. The nano mimic enzyme can promote seed germination and growth, and has a remarkable inhibition effect on watermelon bacterial germs, namely fruit blotch germs or watermelon fungal germs, namely wilt germs.

The utility model belongs to the technical field of forestry epidemic disease detection, and discloses a sampling device for pine wood nematode disease detection, which comprises a U-shaped frame, one side of the U-shaped frame is fixedly connected with a support, one end of a push rod is fixedly connected with a push plate, the other end of the push rod is fixedly connected with a motor, the tail end of a rotor of the motor is coaxially and fixedly connected with a drill bit, and the drill bit is fixedly connected with the support. The clamping assembly comprises a one-way lead screw rotationally connected to one side of the U-shaped frame, the outer portion of the one-way lead screw is in threaded connection with a moving block, clamping plates are symmetrically arranged on the inner side face of the U-shaped frame, a cross rod is fixedly connected to one side of each clamping plate, and the cross rods are in sliding connection with the U-shaped frame; by means of the pine tree sampling device, the problems that due to the fact that the stability during drilling and sampling is difficult to guarantee, the pine tree sampling efficiency is reduced, and damage to pine trees is easily increased are effectively solved.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

The pine wood nematode disease unmanned aerial vehicle monitoring and positioning device comprises an unmanned aerial vehicle body, a mounting block is mounted at the bottom of the unmanned aerial vehicle body, and an auxiliary flight stabilizing structure for improving flight stability by means of airflow is arranged in the mounting block; the bottom of the mounting block is provided with a plugging structure for cooperating with the auxiliary flight stabilization structure, and the bottom of the mounting block is connected with a box body in the middle of the plugging structure. According to the pine wood nematode disease unmanned aerial vehicle monitoring and positioning device and method, cooperative work of the flight stabilizing structure and the blocking and triggering structure is assisted, an airflow channel is optimized, the flight stability of the unmanned aerial vehicle under the conditions of complex terrains and severe weather is enhanced, energy consumption is reduced, and the endurance time is prolonged. In addition, the device is provided with a wireless communication module and a network adapter, and collected and processed data can be transmitted to a ground control station or a cloud server in real time.

The invention discloses a bacillus velezensis strain and application thereof. The invention discloses bacillus velezensis WLH (bacillus velezensis), and the preservation number of the bacillus velezensis WLH is CCTCC (China Center for Type Culture Collection) NO: M 20251238. The bacillus velezensis WLH is screened from rhizosphere of cucumber, the WLH is a biocontrol bacterium, can inhibit growth of pathogenic bacteria of cucumber root rot and fusarium wilt by degrading phenolic acid in soil and promote growth of cucumber plants, and the strain can relieve autotoxicity caused by the phenolic acid, inhibit the cucumber root rot and fusarium wilt and promote growth of cucumber plants. The promotion of crop growth comprises the capability of producing indoleacetic acid (IAA) and the capability of solubilizing phosphate, the promotion of germination of cucumber seeds under the stress of mixed phenolic acid and pathogenic bacteria, and the promotion of growth of cucumber seedlings under the stress of mixed phenolic acid and pathogenic bacteria. Soil continuous cropping obstacles generated in cucumber planting are effectively relieved, and a new basis is provided for microbial control of continuous cropping obstacles.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

This invention discloses a multi-source data fusion early warning and precision application method and system for pine wilt disease, belonging to the field of smart forestry technology. It includes a multi-source data acquisition module, a spatiotemporal data fusion module, a health index calculation module, an external feature extraction module, a dual-channel coupled diagnosis module, a graded early warning module, an application urgency assessment module, an application strategy planning module, and an adaptive optimization module. This invention collects and fuses multi-source data such as satellite remote sensing data, UAV inspection data, ground-based IoT data, and forest meteorological data. It utilizes a dual-channel coupled deep learning model to achieve probabilistic diagnosis of the multidimensional state of individual trees and to extrapolate the spread of the disease. Based on the diagnostic results, it generates graded differentiated application strategies and finally achieves adaptive closed-loop optimization of the calculation parameters and model through a control effect feedback mechanism.

The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing nano-zinc oxide and brucea javanica extract and application of the bactericidal composition, and the mass ratio of the nano-zinc oxide to the brucea javanica extract in active ingredients of the bactericidal composition is 40: 1-1: 40; the bactericidal composition disclosed by the invention has an excellent bactericidal effect, and shows excellent control effects on watermelon fusarium wilt, wheat stem rot, tobacco bacterial wilt, cotton damping-off, cotton damping-off, peanut root rot, peanut stem rot, damping-off, fusarium wilt, damping-off, root rot and the like of fruits, vegetables and beans; the bactericidal composition enhances the bactericidal broad-spectrum property and efficiency, is environment-friendly, does not generate phytotoxicity, is safe to crops, and has a good application prospect.

The present invention belongs to the technical field of antimicrobial polymer materials, and discloses a poly(acrylamide-methacrylamide primary amine salt) with a random structure, a preparation method thereof, and an application thereof. The poly(acrylamide-methacrylamide primary amine salt) with a random structure has a structural formula as shown in Formula 1: #imgabs0# wherein both m and n are positive integers, and m:n=1:(0.1-9). The poly(acrylamide-methacrylamide primary amine salt) with a random structure has a good antibacterial effect, particularly an inhibitory effect on Foc4 spores. After application to soil, it can be stably adsorbed in the soil, with reduced environmental toxicity, and while improving the physical and chemical properties of the soil, it can achieve long-term inhibition of Foc4 spores in the soil, thereby having a good preventive and control effect on plant fungal diseases such as banana wilt.

This invention provides compounds and methods of application that possess both broad-spectrum antifungal activity and plant-induced resistance, belonging to the field of agricultural technology. It addresses the problem that "traditional pure immune inducers must be applied before disease onset, making it difficult to meet the full-cycle control needs of disease prevention and treatment in the field." Compared to the previously developed PC1 and PC20, the two compounds of this invention, 2,4-dibromo-6-nitrophenol and 7-deazon-2'-deoxy-7-iodoadenosine, can both inhibit the growth of rice blast fungus and significantly improve rice resistance to rice blast through foliar spraying, meeting the full-cycle control needs of disease prevention and treatment in the field. Furthermore, these two compounds can directly inhibit the growth of various fungal pathogens such as peanut white mold, tomato gray mold, wheat scab, rice bakanae disease, rice false smut, mango malformation, and banana wilt.

The application provides application of a bacillus subtilis K8 nano-seleniumized active bacterium agent in prevention and treatment of plant pathogenic bacteria, and belongs to the technical field of biological prevention and treatment of plant diseases. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent is obtained by fermenting and culturing bacillus subtilis K8 in a fermentation medium containing sodium selenite. Bacillus subtilis The bacillus subtilis K8 nano-seleniumized active bacterium agent has a strong prevention and treatment effect on cucumber fusarium wilt, cucumber botrytis blight, tomato early blight and tomato root rot, provides an efficient, safe and practical method for prevention and treatment of plant fungal diseases, has low cost, simple preparation process, is convenient for industrialization, and has good development value and market prospect.

The invention discloses a preparation method and application of a pesticide preparation for preventing and treating watermelon fusarium wilt, and belongs to the technical field of biocide pesticides. The carbendazim, the iprodione, the lotus seed pot extract and the seaweed residues are combined to serve as effective components of the pesticide, the pesticide preparation for preventing and treating the watermelon fusarium wilt is applied to soil which is continuously seeded for many years according to field experiment data, the disease index is 7.22%, the prevention effect is 82.44%, the yield of a single plant is 9.75 + / -0.35 kg, and the yield per mu is 2865 kg. The seaweed residues and the lotus seed pot extract are mixed to serve as a pesticide synergist, the pesticide synergist is compounded with a basic chemical agent, the obtained pesticide preparation has a better effect in the aspect of preventing and treating watermelon fusarium wilt, and the combined effect is better than the effect of independently using the basic chemical agent, the seaweed residues or the lotus seed pot extract; the method has practical significance in watermelon fusarium wilt control and watermelon yield increase.

The invention belongs to the technical field of molecular biology, and discloses a molecular marker remarkably related to cassava bacterial wilt resistance and application of the molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to cassava bacterial wilt resistance, which is characterized in that the sequence of the SNP molecular marker is shown as SEQ ID NO: 3, the SNP site is positioned at the 392nd site from the 5'terminal of the sequence shown as SEQ ID NO: 3, and the polymorphism of the SNP molecular marker is A / T. By detecting the genotype of the SNP molecular marker, cassava with strong resistance to bacterial wilt can be accurately selected, and the SNP molecular marker can be effectively used for cassava molecular marker assisted breeding; according to actual breeding requirements, genotype identification can be carried out on cassava, genotype cassava with high bacterial wilt resistance is selected for hybridization, cassava filial generation with high bacterial wilt resistance is obtained, breeding time is saved, cost is low, accuracy is high, and the cassava breeding process is accelerated.

A remote-controlled chainsaw comprises fixing means e.g., a chain 2, attached to a triangular baseplate for fixing to a tree 1. One or more supports 3 are connected to the chain 2 and a pair of rails 4 is mounted on the supports 3 with a control platform 7 movably mounted on the rails 4. There may be a separate chain tensioner. A chainsaw holder 5a is mounted on the control platform 7 and a chainsaw 5 is connected to the holder, with a chainsaw blade 6 extending therefrom. A motor moves the control platform 7 relative to the pair of rails 4 to effect cutting the tree. Following an initial partial cut, the rails may be manually or motor tilted about a horizontal axis 10 to enable an inclined cut to form a notch or birdmouth in the tree stem. The saw may then be rotated and moved on the rails to the other side of the tree to perform a felling cut. Allows remote cutting to improve safety when felling diseased trees e.g., with chalara, ash-dieback without the need for heavy equipment.

The application provides bacillus velezensis LB17, K87, MP6 and a compound microbial inoculant thereof, a preparation method and application, and belongs to the technical field of biocontrol bacteria.The application collects rhizosphere and rhizosphere soil of characteristic crops such as highland barley, lily, tomato and pepper in the Gansu-Qinghai region, and obtains 305 pure cultures through separation, obtains three strains of bacillus velezensis LB17, K87 and MP6 through multiple screening, and finally obtains a compound fermentation microbial inoculant, i.e., tuoweiwei No.7, which is fermented by the three strains of bacillus velezensis LB17, K87 and MP6 through optimization of inoculation amount, components of culture medium, ratio and fermentation conditions.Tuoweiwei No.7 can effectively prevent plant diseases such as pathogenic microorganisms, root rot and fusarium wilt, and can promote plant growth and improve the stress resistance of plants.The compound microbial inoculant provided by the application provides a strong guarantee for the prevention and control of root rot diseases of special habitat crops in the Gansu-Qinghai region.

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.

The present invention belongs to the technical field of the application of soil microorganisms and the prevention and control of crop diseases. The present invention provides the application of Streptomyces morookaense strain SGX33 in the preparation of a microbial fertilizer for preventing and controlling plant diseases, wherein the plant diseases are wheat root rot or banana wilt. The fertilizer containing Streptomyces morookaense strain SGX33 of the present invention increases the plant height of wheat seeds in soil containing wheat root rot pathogens, and can basically reach the growth level of wheat seeds in soil without pathogens. At the same time, for fields with a banana wilt incidence rate of more than 50% in the previous year, when bananas are replanted, the prevention and control of banana wilt reaches more than 75%, and the yield increases by more than 80%.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The present invention discloses a method for preparing nanosilver particles using rose extract, a product, and an application thereof, belonging to the technical field of biosynthetic nanomaterials. The method comprises the following steps: (1) washing, drying, and crushing rose petals, mixing them with deionized water, stirring them evenly, letting them stand, and filtering them thoroughly to obtain a rose extract; (2) mixing the rose extract obtained in step (1) with an AgNO3 solution, reacting them in the dark to obtain a mixed solution, and centrifuging, washing, and vacuum freeze-drying the mixed solution to obtain the nanosilver particles. The nanosilver particles obtained by the method of the present invention have a small average particle size, uniform size, and stable structure, and can effectively inhibit the activity of Pseudomonas aeruginosa and prevent and control plant diseases caused by it. The nanosilver particles obtained by the present invention are used as agricultural fungicides in the prevention and control of bayberry wilt disease, and have a good prevention and control effect.

The invention relates to the field of plant genetic engineering, and discloses a corn sheath blight disease-resistant gene GRMZM2G057329 and application thereof, and the corn sheath blight disease-resistant gene GRMZM2G057329 comprises any one of the following sequences: a nucleotide sequence shown as SEQ ID No.1, a nucleotide sequence shown as SEQ ID No.2, a nucleotide sequence shown as SEQ ID No.3, a nucleotide sequence shown as SEQ ID No.4, a nucleotide sequence shown as SEQ ID No.5, a nucleotide sequence shown as SEQ ID No.6, a The nucleotide sequence is as shown in SEQ ID No.2; the amino acid sequence of the protein is shown as SEQ ID No.3; the nucleotide sequence has 90% or more of identity with the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 and is used for coding protein with the same function as the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2. According to the present invention, the DNA fragment of the complete coding segment of the anti-disease gene GRMZM2G057329 separated and cloned from corn is adopted, and the GRMZM2G057329 gene expression quantity is increased so as to significantly enhance the sheath blight resistance of the corn genetic transformation plant;