52 results about "Microbial disease" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a multi-channel optical fiber biosensor based on fluorescence detection, optical fiber sensing units can be cascaded on the same optical fiber, and the sensing units are realized through a packaging structure adopting 3D printing resin. Quantum dot modification, functionalization processing and sensing detection on the surface of the sensing area of the optical fiber sensing unit can be completed in the packaging structure, and integration is achieved. Different antibody materials can be modified on quantum dot materials with different particle sizes so as to obtain fluorescence signals with different emission wavelengths, and qualitative and quantitative detection of various microorganisms and viruses is realized according to the variation of fluorescence intensity of a corresponding emission peak. The sensor is low in cost, short in preparation time and simple in signal acquisition and processing, the packaging structure can ensure the stability of the sensor, the sensor is convenient to carry, and the sensor can be applied to the environmental protection field such as outdoor water quality real-time monitoring.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.

The invention provides a small molecule compound capable of inducing plant immune response and improving plant disease resistance. Specifically, it is found for the first time that the 2'c ADPR shown in the formula I or the analogue thereof, or the pharmaceutically acceptable salt thereof can significantly enhance the resistance of plants to pathogenic microorganisms, diseases and pests and viruses.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

A Toll-like receptor (TLR) is an indispensable component of an innate immune system and plays a key role in identifying and coping with microbial pathogens. TLR7, TLR8, and TLR9 are located in the endosome-lysosome compartment within the cell and then exclusively used to detect nucleic acids of a microorganism after the microorganism is swallowed and reaches the endosome compartment. The biological importance of the TLR agonists lies in that they can be used as powerful tools for studying innate immune responses, developing vaccines and potential therapeutic applications. In addition, TLR agonists have been explored for use as immunotherapy, while these TLR antagonists can be used as new pathogenic nodes in the context of different autoimmune diseases. Therefore, the agonists and antagonists of the endosomal TLR have therapeutic significance in different clinical backgrounds. The previous study on 8-oxo adenines shows that the C-6-site-NH2 group is extremely important for the recognition of agonistic active sites and the subsequent induction of interferon (IFN). The present invention explores the ability of a hydrogen-substituted 8-oxopurine derivative that does not contain an essential C-6 amine group. The C-6 amino group in the 8-oxopurine derivative is substituted by hydrogen (H), providing an exciting opinion for regulating the endosome TLRs (Toll-Like Receptors). The ability of 8-oxopurine derivatives to modulate the endosomal TLR has been reported, which derivatives are related / interrelated to various substitutions at N-7, N-9 and C-2, all derivatives containing hydrogen at C-6. Structure 1

The invention discloses an ancient building wood structure microenvironment multi-mode monitoring in-situ intervention protection system and method, and relates to the technical field of ancient building protection. The system comprises a data acquisition module used for acquiring multi-modal data of a surrounding environment of a target wood structure; the intervention execution module is used for performing physical intervention protection on the microenvironment around the target wood structure; and the edge controller is in communication connection with the data acquisition module and the intervention execution module, and is used for receiving the multi-modal data, processing and judging the multi-modal data based on a preset trigger rule, and sending a control instruction to the intervention execution module to start a corresponding intervention operation when judging that a trigger condition is met. According to the invention, monitoring data can be automatically processed in real time, intervention measures are triggered instantly when preset conditions are met, the response time is shortened from a'day / hour 'level to a'minute' level, and'early discovery and early inhibition 'of microbial diseases and insect pests are realized.

Provided is use of an endoperoxide in preparing an anti-microbial drug. In an anoxic condition, the endoperoxide can partially and effectively inhibit the survival of Mycobacterium tuberculosis. Provided is use of an endoperoxide in combination with a respiratory chain inhibitor in preparing an anti-microbial drug. The endoperoxide includes artemisinin and derivatives thereof, and other endoperoxides with a two-oxygen bridge structure. The endoperoxide has synergistic effects with a variety of respiratory chain inhibitors, and the combination has a more effective inhibition effect on Mycobacterium tuberculosis.

The invention relates to the technical field of pathogen optical detection, and discloses a multiple immunofluorescence analysis method for detecting multiple microbial pathogens. The method comprises the following steps: acquiring multi-modal fluorescence data of a sample surface through a bionic visual perception system, and generating a pathogen surface distribution heat map by using a deep neural network to identify a high-risk area. The system dynamically regulates and controls an optical detection array to emit a coding light signal into a sample according to a region division result, and reconstructs an internal three-dimensional structure of a pathogen by analyzing fluorescence response. Surface and internal data are integrated through a space-time alignment mechanism, fusion analysis is carried out by adopting a graph neural network, and a detection confidence score is generated to drive an adaptive decision. According to the method, accurate and dynamic detection of pathogens from the surface to the interior is realized, and the detection depth and the result reliability of a hidden target in a complex sample are improved.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

An endoscopic system is disclosed. The endoscopic system includes an endoscope having a handle and an elongated flexible member configured to be removed from the handle and reprocessed between procedures. Meanwhile, the handle is cleaned and sanitized and disposed in a sanitizer for sterilizing destroying all microorganisms, viruses, and / or bacteria present on the handle. Once sanitized or sterilized, the handle may be fixed to a new removable elongated flexible member for another endoscopy procedure.

The invention belongs to the technical field of microbial viruses, and particularly relates to a culture method for improving the virus titer of duck flavivirus, which comprises the following steps: step 1, preparing a culture solution, accurately weighing RPMI1640 dry powder, dissolving the RPMI1640 dry powder in three-distilled water, then adding a proper amount of fetal calf serum and double antibodies, filtering and sterilizing, then sub-packaging into sterile culture bottles, and preparing the culture solution for later use; storing at a proper temperature for later use; 2, preparing cells, selecting suitable cells, inoculating the cells into a culture flask, and carrying out single-layer culture by using an RPMI1640 culture solution to ensure that the cells are in a good growth state before the cells are inoculated with viruses, The method has the beneficial effects that by selecting a suitable host cell line, the cells are ensured to be in a healthy state, the performance reduction caused by high algebraic culture is avoided, and the cell quality is improved. In addition, the cell culture medium suitable for virus production is selected, and the culture medium with high transfection efficiency and cell maintaining capacity after transfection can remarkably improve the virus titer.

The invention discloses a sputum sample pretreatment device for microbial etiology inspection, and relates to the technical field of microbial etiology inspection. Comprising a machine shell, a bottom plate is arranged on the bottom wall of an inner cavity of the machine shell, a lifting motor unit and an ultrasonic unit are arranged on the bottom plate, a positioning plate is connected to the lifting motor unit, a proximity switch unit is arranged on the positioning plate, the proximity switch unit is electrically connected with the ultrasonic unit, a placing block is arranged on the ultrasonic unit, and the placing block is electrically connected with the ultrasonic unit. A sputum taking cup containing cavity is formed in the containing block, a clamping mechanism is arranged on the outer side of the sputum taking cup containing cavity, and the clamping mechanism is connected with the lifting motor unit through a connecting rod. The ultrasonic unit generates ultrasonic waves to quickly and effectively homogenize the phlegm reducing liquid, so that the detection efficiency of microbial etiology inspection is improved, and the pretreatment cost is reduced.

The present invention relates to a combination therapy for treating infections caused by bacteria of the genus Mycobacterium and other microbial pathogens in subjects with cystic fibrosis comprising the sequential, simultaneous or separate administration of pyridine-2-thiol 1-oxides or their salts and cystic fibrosis transmembrane conductance regulator (CFTR) modulator compounds.

The invention, which provides monitoring of organic / inorganic, biological factors that positively / negatively affect water, air, soil quality, determination and qualitative and quantitative measurement of the concentration of micro-organisms, viruses, cancer biomarkers analysed in human and animal samples, monitoring of biomolecular interactions by various modifications on the surface of a biomaterial that has not been used as a biosensor electrode, relates to an electrochemical impedimetric, voltametric, amperometric and piezoelectric biosensor for the detection of cells, microorganisms, viruses, DNA, RNA proteins and enzymes in human / animal samples and for the detection of microorganisms and biological / chemical markers in liquid, food and environmental samples.

Methods and devices for continuous flow monitoring of a liquid sample for presence of an airborne microbial pathogen are provided. The liquid sample can be derived from environmental air. The methods and devices provide for continuous labeling of a targeted pathogen in the liquid sample with a fluorescent probe. A customized fluorescence detector is provided that can detect labeled pathogens during continuous flow.

The invention relates to the technical field of data processing, in particular to a method for extracting the relationship between diseases and intestinal microorganisms based on a transfer learning model, which comprises the following steps: preprocessing collected related literature data to obtain a training set, a verification set and a test set; training a pre-training model by using the training set, the verification set and the test set to obtain a relationship extraction model; and performing relation extraction through the relation extraction model to obtain a final relation category. According to the method, the BERE framework of transfer learning is used for performing relation extraction on the diseases and the intestinal microorganisms, so that the correlation between the diseases and the intestinal microorganisms is researched from a comprehensive perspective, and the potential correlation is mined. The application of the method not only is expected to deepen the understanding of disease mechanisms, but also provides a powerful tool for future medical research, and promotes the development of personalized medical treatment and treatment strategies. The problem that no related technology for extracting the relation of the microbial diseases exists at present is solved.

Antimicrobial compositions include a therapeutically effective amount of neem oil, extract of the plant Hypericum spp., polyunsaturated fatty acids (PUFAs), carrageenans, and / or chlorhexidine; and (optionally) in combination with one or more pharmaceutically acceptable carriers, additives, and / or diluents. The antimicrobial compositions are effective in treating or preventing upper respiratory tract infections, preferably upper respiratory tract infections caused by human Coronaviruses and are included within methods of treating or preventing infection of the upper respiratory tract of humans by a microbial pathogen.

Provided herein are compounds of general Formula I:or agriculturally acceptable salts thereof. The compounds of Formula I can be used to improve the health of plants. For example, the compounds of Formula I can be used to inhibit a microbial pathogen of a plant, or to increase resistance of a plant to one or more abiotic stress. Methods of manufacturing the compounds of general Formula I, as well as synthetic intermediates are also provided.

The present invention aims to provide a novel triterpenoid saponin vaccine adjuvant. By means of molecular design, synthesis, activity evaluation, and other means, the adjuvant significantly improves immunostimulatory activity thereof while reducing the potential toxicity thereof and significantly enhancing the stability of a product. Specifically, the adjuvant of the present invention can induce a Th1 / Th2 mixed immune response, effectively improve the ability of an antigen presenting cell (such as a dendritic cell) to uptake, treat, and present an antigen (including but not limited to a microbial pathogen, a cancer cell, etc.), thereby enhancing the cellular immune efficacy and exhibiting great potential for preventing and treating a disease caused by a related pathogen.

A grade of a specific microbial disease in a biological sample of a subject is determined. In one example, masked microbial reference genomes are used to determine the amount of free DNA molecules corresponding to a particular microbial species associated with a particular microbial disease. The masking may remove an area shared with another. In another example technique, end motifs are used for free DNA fragments from a subject and from a particular microbial species. A correlation between a subject and an amount of a set of terminal sequence motifs of a particular microbial species may be determined. The two groups of quantities of positive subjects are substantially more correlated than negative subjects.

The invention discloses a normal-temperature self-regeneration type hierarchical porous MOF composite material and a preparation method and application thereof. The composite material comprises an MIL-101 structure MOF carrier constructed by Fe < 3 + > and terephthalic acid, a nanometer MnO2-Ce0 heterojunction catalytic component, a quaternary ammonium salt cationic polymer molecular brush, a catalyst carrier, a catalyst carrier, a catalyst carrier, a catalyst carrier, a catalyst carrier and a catalyst carrier. The composite material can realize adsorption and catalytic decomposition of gaseous pollutants and disinfection of microbial pathogens under normal-temperature and dark conditions, and the composite material can be recycled and regenerated, so that the comprehensive use cost is reduced. The preparation of the composite material is completed through microwave synthesis of MOF, in-situ loading of heterojunction, covalent grafting of molecular brushes and vacuum drying, the process is mild, and the cost is low.

This invention provides a means to inactivate the inherent toxicity of tetanus toxin and produce a safe and effective vaccine. [Solution] This specification provides engineered non-catalytic, non-toxic tetanus toxin variants and methods for using such engineered tetanus toxin variants as low-dose prophylactic vaccines that are more effective than non-toxic and their respective chemically inactivated toxoids. In addition, this specification provides conjugate vaccine carriers containing engineered tetanus toxin variants and methods for using such conjugate vaccines to induce a T-cell-dependent immunomemory response that can target a broad range of microbial pathogens as a single vaccine.

The application discloses a microbial disease diagnosis integrated learning method for promoting diversity and keeping distribution, and mainly relates to the technical field of microorganisms, and aims to solve the defects of the resampling method, the limitations of the deep learning method in small sample and extreme imbalance scenes, and the problems of the traditional integrated learning method in diversity, computational overhead and distribution keeping. The method comprises the following steps: determining the corresponding base classifier according to the class imbalance rate of the candidate balanced subset; evaluating the classification accuracy and error rate by using the prediction probability, and constructing an effective base model pool corresponding to the balanced subset; determining the weight matrix corresponding to the base classifier by using the candidate balanced subset; pruning the effective base model pool of the base classifier by using the weight matrix to obtain a pruned base classifier; and performing weighted integration processing on the pruned base classifier by using a weighted fusion mechanism, and outputting a prediction result by using the comprehensive quality score of each model of the pruned base classifier.

Fluidic devices and related methods are generally provided. The fluidic devices described herein may be useful, for example, for diagnostic purposes (e.g., detection of the presence of one or more disease causing bacteria in a patient sample). Unlike certain existing fluidic devices for diagnostic purposes, the fluidic devices and methods described herein may be useful for detecting the presence of numerous disease causing bacteria in a patient sample substantially simultaneously (e.g., in parallel). In some embodiments, the fluidic devices and methods described herein provide highly sensitive detection of microbes in relatively large fluidic samples (e.g., between 0.5 mL and about 5 mL), as compared to certain existing fluidic detection (e.g., microfluidic) devices and methods. In an exemplary embodiment, increased detection sensitivity of microbial pathogens present in a patient sample (e.g., blood) is performed by selectively removing human nucleic acid prior to sensitive detection of microbial infection. In some embodiments, the fluidic device allows for the identification of microbial pathogens directly from unprocessed blood without having to conduct blood culturing processes.

This invention relates to the technical field of microbial disease control, specifically to a composition and method for controlling mango rapid death disease. The composition comprises, by weight percentage: 0.05%–0.5% polyamine mixture, 1.0%–10.0% *Lactobacillus plantarum* component, 0.01%–0.2% stabilizer, 0.1%–2.0% penetration enhancer, 1.0%–5.0% slow-release carrier, 0.05%–0.5% pH adjuster, and a carrier or diluent to bring the total to 100%. The mass ratio of polyamine to *Lactobacillus plantarum* component is 10:1 to 100:1. This composition is applied to mango plants through root irrigation or soil pinhole injection, effectively inhibiting... Fusarium The pathogenic fungus activates systemic resistance in plants and rebuilds the rhizosphere microecology. Field trial results show that the composition of this invention significantly reduces the incidence of disease, enhances root vitality and plant growth rate, and is superior to existing commercially available control agents. It is suitable for the promotion and use of green agricultural disease control.

The application discloses a kind of photocatalyst for killing microorganism on the surface of stone cultural relics and inhibiting its growth, the catalyst is BiVO4 / TiO2-Ti2N (MXene) ternary photocatalytic material;Scanning electron microscope is observed to 20 times to the catalytic material is enlarged, Ti2N (MXene) is two-dimensional layered structure, and TiO2 is attached to the surface of Ti2N (MXene), BiVO4 is spherical structure and is dispersed in the surface and around of Ti2N (MXene) and TiO2.The photocatalyst of the application is coated on the surface of stone cultural relics, not only can effectively kill microorganism on the surface of stone cultural relics and inhibit the growth of microorganism, but also can improve the hydrophobic property of stone cultural relics, to realize the effective preventive protection of stone cultural relics.The photocatalyst of the application has simple manufacturing process, mild reaction condition, low cost, is suitable for mass production and widely used in the preventive protection of stone cultural relics microbial disease.

The application relates to the technical field of microorganism detection, in particular to a medical microorganism virus detection device which comprises a base, further comprises a mounting unit, the mounting unit comprises mounting seats arranged in an array on the base, the middle part of each mounting seat is provided with a suction channel, the middle part of the suction channel is provided with an adjusting piece, a connecting piece is connected with the mounting seat at one end and connected with a filtering unit at the other end, a chamber is arranged in the interior of the mounting seat and communicates with the adjusting piece through a connecting hole, a moving piece is arranged in the middle part of the chamber and can vertically displace along the chamber, the cross section of the suction channel is dynamically adjusted by regulating the moving piece, the challenge of suction filtration of different viscosity samples is effectively coped with, the cross section of the channel is increased and the filter membrane is periodically pushed for high-viscosity samples, the flow is improved and the channel is prevented from being blocked, the cross section of the channel is reduced and the filter membrane is caused to be concave for low-viscosity samples, the filtering resistance is increased, the microorganism loss is reduced, and the detection accuracy is ensured.

The invention provides an active polypeptide and application thereof in culture of tegillarca granosa, and after amino acid optimization, the provided active polypeptide has a remarkable inhibition effect on common microorganisms such as vibrio and the like in aquaculture. The active polypeptide is further prepared into microcapsules with the particle size suitable for filter feeding of juvenile tegillarca granosa, and the microcapsules are fed to the tegillarca granosa, so that microbial diseases occurring in tegillarca granosa breeding are effectively reduced. The amino acid sequence of the polypeptide is SEQ ID NO: 2. The active polypeptide obtained by screening comes from pearl oyster, the amino acid sequence of the provided active polypeptide is optimized, and the active polypeptide has good antibacterial activity on vibrio harveyi. After high expression of the active polypeptide, the active polypeptide is prepared into the microcapsule with the particle size suitable for filter feeding of juvenile shellfish of tegillarca granosa, the growth of the tegillarca granosa is promoted, and diseases caused by vibrio harveyi are reduced.