75 results about "Microbial disease" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a multi-channel optical fiber biosensor based on fluorescence detection, optical fiber sensing units can be cascaded on the same optical fiber, and the sensing units are realized through a packaging structure adopting 3D printing resin. Quantum dot modification, functionalization processing and sensing detection on the surface of the sensing area of the optical fiber sensing unit can be completed in the packaging structure, and integration is achieved. Different antibody materials can be modified on quantum dot materials with different particle sizes so as to obtain fluorescence signals with different emission wavelengths, and qualitative and quantitative detection of various microorganisms and viruses is realized according to the variation of fluorescence intensity of a corresponding emission peak. The sensor is low in cost, short in preparation time and simple in signal acquisition and processing, the packaging structure can ensure the stability of the sensor, the sensor is convenient to carry, and the sensor can be applied to the environmental protection field such as outdoor water quality real-time monitoring.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.

The present invention provides an improved method and system for identifying nucleic acid sequences within a set of sequences obtained by a sequencer, constructing database according to the identification of nucleic acid sequences, identifying different genus, species, sub-species, serotypes, variety of microorganism, virus, genes, or nucleic acid sequences of interest, for its use in the field of molecular biology applied to diagnosis, in hospitals, schools, industry or any place wherein this method and system is required to identify nucleic acid sequences, obtained by a sequencer. Specifically, the present invention provides an improved method and system that allow sequences to be differentiated from data obtained by nucleic acid sequencing.

The invention provides a small molecule compound capable of inducing plant immune response and improving plant disease resistance. Specifically, it is found for the first time that the 2'c ADPR shown in the formula I or the analogue thereof, or the pharmaceutically acceptable salt thereof can significantly enhance the resistance of plants to pathogenic microorganisms, diseases and pests and viruses.

The invention provides a test strip for identifying gram-negative bacteria in a peritoneal dialysis solution and a preparation method, and relates to the technical field of test strips. The test strip comprises a bottom plate as well as a sample pad, a combination pad, a coating pad and a water absorption pad which are sequentially arranged on the bottom plate, wherein a polydopamine coated colloidal gold coupled antibiotic probe is sprayed on the combination pad; a detection line is arranged on the coating pad, and the detection line is formed by hydrogel coated with antibiotics. According to the non-antibody-dependent immunochromatography test paper for detecting the gram-negative bacteria in the peritoneal dialysis solution, provided by the invention, a marker is simple and convenient to prepare, the use of paired antibodies is avoided, and the cost of the test paper is further reduced; moreover, time is gained for clinical diagnosis of microbial pathogens in dialysate, and correspondingly, antibiotic treatment is carried out on a patient in time. Each detection process can be completed within 10 min, the detection result is accurate, and the gram-negative bacteria in a peritoneal dialysis solution sample can be rapidly detected.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

The present invention provides compositions and methods for controlling iron trapping pathogens. In a preferred embodiment, the invention can be applied to an environment in which pathologic microorganisms grow, where the microorganisms pathologically capture iron to control the pathogens by fighting for iron resources with the pathogens.

A Toll-like receptor (TLR) is an indispensable component of an innate immune system and plays a key role in identifying and coping with microbial pathogens. TLR7, TLR8, and TLR9 are located in the endosome-lysosome compartment within the cell and then exclusively used to detect nucleic acids of a microorganism after the microorganism is swallowed and reaches the endosome compartment. The biological importance of the TLR agonists lies in that they can be used as powerful tools for studying innate immune responses, developing vaccines and potential therapeutic applications. In addition, TLR agonists have been explored for use as immunotherapy, while these TLR antagonists can be used as new pathogenic nodes in the context of different autoimmune diseases. Therefore, the agonists and antagonists of the endosomal TLR have therapeutic significance in different clinical backgrounds. The previous study on 8-oxo adenines shows that the C-6-site-NH2 group is extremely important for the recognition of agonistic active sites and the subsequent induction of interferon (IFN). The present invention explores the ability of a hydrogen-substituted 8-oxopurine derivative that does not contain an essential C-6 amine group. The C-6 amino group in the 8-oxopurine derivative is substituted by hydrogen (H), providing an exciting opinion for regulating the endosome TLRs (Toll-Like Receptors). The ability of 8-oxopurine derivatives to modulate the endosomal TLR has been reported, which derivatives are related / interrelated to various substitutions at N-7, N-9 and C-2, all derivatives containing hydrogen at C-6. Structure 1

The present application discloses a use of an endoperoxy compound in the preparation of an antimicrobial pathogenic bacteria drug. Under anaerobic conditions, in the culture medium of Mycobacterium tuberculosis, the representative artemisinin-based drug of the endoperoxy compound can partially and effectively inhibit the survival of Mycobacterium tuberculosis. The present application also discloses a use of an endoperoxy compound in combination with a respiratory chain inhibitor in the preparation of an antimicrobial pathogenic bacteria drug. Under normal and anaerobic conditions, in the culture medium of Mycobacterium tuberculosis, the endoperoxy compound and a variety of respiratory chain inhibitors have a synergistic effect, and the combined use has a more effective inhibitory effect on Mycobacterium tuberculosis. The endoperoxy compounds include artemisinin and its derivatives, as well as other endoperoxy compounds with a double oxygen bridge structure, which may have a similar mechanism of action, and the internal oxygen bridge is the key group for them to play a role. The present application aims to kill pathogens more quickly or effectively, improve the cure efficiency or shorten the course of treatment, overcome the problem of drug resistance, and reduce the amount of drugs to reduce side effects on patients by introducing endoperoxy compounds represented by artemisinin as drugs.

The invention discloses an ancient building wood structure microenvironment multi-mode monitoring in-situ intervention protection system and method, and relates to the technical field of ancient building protection. The system comprises a data acquisition module used for acquiring multi-modal data of a surrounding environment of a target wood structure; the intervention execution module is used for performing physical intervention protection on the microenvironment around the target wood structure; and the edge controller is in communication connection with the data acquisition module and the intervention execution module, and is used for receiving the multi-modal data, processing and judging the multi-modal data based on a preset trigger rule, and sending a control instruction to the intervention execution module to start a corresponding intervention operation when judging that a trigger condition is met. According to the invention, monitoring data can be automatically processed in real time, intervention measures are triggered instantly when preset conditions are met, the response time is shortened from a'day / hour 'level to a'minute' level, and'early discovery and early inhibition 'of microbial diseases and insect pests are realized.

According to the penaeus vannamei boone source bdellovibrio bacteriovorus and the application thereof, the screened bdellovibrio bacteriovorus has splitting property on pathogenic bacteria such as vibrio harveyi and edwardsiella and does not exist on penaeus vannamei boone, so that the culture benefit is improved. The preservation number of the bdellovibrio bacteriovorus provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M20241363. The screened bdellovibrio Bde2 strain can widely split pathogenic bacteria of the penaeus vannamei, has a better antibacterial effect than a standard strain, does not have pathogenicity to the penaeus vannamei, can be used for a long time in the culture production of the penaeus vannamei, and particularly can be used for preventing microbial diseases of the penaeus vannamei in high-temperature seasons.

Provided is use of an endoperoxide in preparing an anti-microbial drug. In an anoxic condition, the endoperoxide can partially and effectively inhibit the survival of Mycobacterium tuberculosis. Provided is use of an endoperoxide in combination with a respiratory chain inhibitor in preparing an anti-microbial drug. The endoperoxide includes artemisinin and derivatives thereof, and other endoperoxides with a two-oxygen bridge structure. The endoperoxide has synergistic effects with a variety of respiratory chain inhibitors, and the combination has a more effective inhibition effect on Mycobacterium tuberculosis.

The invention relates to the technical field of pathogen optical detection, and discloses a multiple immunofluorescence analysis method for detecting multiple microbial pathogens. The method comprises the following steps: acquiring multi-modal fluorescence data of a sample surface through a bionic visual perception system, and generating a pathogen surface distribution heat map by using a deep neural network to identify a high-risk area. The system dynamically regulates and controls an optical detection array to emit a coding light signal into a sample according to a region division result, and reconstructs an internal three-dimensional structure of a pathogen by analyzing fluorescence response. Surface and internal data are integrated through a space-time alignment mechanism, fusion analysis is carried out by adopting a graph neural network, and a detection confidence score is generated to drive an adaptive decision. According to the method, accurate and dynamic detection of pathogens from the surface to the interior is realized, and the detection depth and the result reliability of a hidden target in a complex sample are improved.

Novel compositions are described, each comprising at least one active constituent that has a 12-carbon atom backbone as a 12-carbon chain base structure having at least a sub-terminal nitrogen and no terminal amino group. One of the active constituents is Boc-12-Ado-OH (BOA) and / or N,N-dimethyldodecylamine N-oxide (LO). Novel particle or liposomal compositions comprising at least BOA and / or LO may be prepared with an emulsifier and / or lipid and optionally a biopolymer, and may be formulated into pharmaceutically acceptable formulations and / or medicaments with or without one or more pharmaceutically acceptable carriers or excipients for utilization as an antimicrobial against one or more microbial pathogens. The novel antimicrobial compositions have broad-spectrum growth inhibitory or other destructive activity against microbial pathogens, biofilms produced by one or more pathogens, and biofilm infections.

An endoscopic system is disclosed. The endoscopic system includes an endoscope having a handle and an elongated flexible member configured to be removed from the handle and reprocessed between procedures. Meanwhile, the handle is cleaned and sanitized and disposed in a sanitizer for sterilizing destroying all microorganisms, viruses, and / or bacteria present on the handle. Once sanitized or sterilized, the handle may be fixed to a new removable elongated flexible member for another endoscopy procedure.

The invention belongs to the technical field of microbial viruses, and particularly relates to a culture method for improving the virus titer of duck flavivirus, which comprises the following steps: step 1, preparing a culture solution, accurately weighing RPMI1640 dry powder, dissolving the RPMI1640 dry powder in three-distilled water, then adding a proper amount of fetal calf serum and double antibodies, filtering and sterilizing, then sub-packaging into sterile culture bottles, and preparing the culture solution for later use; storing at a proper temperature for later use; 2, preparing cells, selecting suitable cells, inoculating the cells into a culture flask, and carrying out single-layer culture by using an RPMI1640 culture solution to ensure that the cells are in a good growth state before the cells are inoculated with viruses, The method has the beneficial effects that by selecting a suitable host cell line, the cells are ensured to be in a healthy state, the performance reduction caused by high algebraic culture is avoided, and the cell quality is improved. In addition, the cell culture medium suitable for virus production is selected, and the culture medium with high transfection efficiency and cell maintaining capacity after transfection can remarkably improve the virus titer.

The invention discloses a sputum sample pretreatment device for microbial etiology inspection, and relates to the technical field of microbial etiology inspection. Comprising a machine shell, a bottom plate is arranged on the bottom wall of an inner cavity of the machine shell, a lifting motor unit and an ultrasonic unit are arranged on the bottom plate, a positioning plate is connected to the lifting motor unit, a proximity switch unit is arranged on the positioning plate, the proximity switch unit is electrically connected with the ultrasonic unit, a placing block is arranged on the ultrasonic unit, and the placing block is electrically connected with the ultrasonic unit. A sputum taking cup containing cavity is formed in the containing block, a clamping mechanism is arranged on the outer side of the sputum taking cup containing cavity, and the clamping mechanism is connected with the lifting motor unit through a connecting rod. The ultrasonic unit generates ultrasonic waves to quickly and effectively homogenize the phlegm reducing liquid, so that the detection efficiency of microbial etiology inspection is improved, and the pretreatment cost is reduced.

The present invention relates to a combination therapy for treating infections caused by bacteria of the genus Mycobacterium and other microbial pathogens in subjects with cystic fibrosis comprising the sequential, simultaneous or separate administration of pyridine-2-thiol 1-oxides or their salts and cystic fibrosis transmembrane conductance regulator (CFTR) modulator compounds.

The invention, which provides monitoring of organic / inorganic, biological factors that positively / negatively affect water, air, soil quality, determination and qualitative and quantitative measurement of the concentration of micro-organisms, viruses, cancer biomarkers analysed in human and animal samples, monitoring of biomolecular interactions by various modifications on the surface of a biomaterial that has not been used as a biosensor electrode, relates to an electrochemical impedimetric, voltametric, amperometric and piezoelectric biosensor for the detection of cells, microorganisms, viruses, DNA, RNA proteins and enzymes in human / animal samples and for the detection of microorganisms and biological / chemical markers in liquid, food and environmental samples.

Methods and devices for continuous flow monitoring of a liquid sample for presence of an airborne microbial pathogen are provided. The liquid sample can be derived from environmental air. The methods and devices provide for continuous labeling of a targeted pathogen in the liquid sample with a fluorescent probe. A customized fluorescence detector is provided that can detect labeled pathogens during continuous flow.

The invention relates to the technical field of data processing, in particular to a method for extracting the relationship between diseases and intestinal microorganisms based on a transfer learning model, which comprises the following steps: preprocessing collected related literature data to obtain a training set, a verification set and a test set; training a pre-training model by using the training set, the verification set and the test set to obtain a relationship extraction model; and performing relation extraction through the relation extraction model to obtain a final relation category. According to the method, the BERE framework of transfer learning is used for performing relation extraction on the diseases and the intestinal microorganisms, so that the correlation between the diseases and the intestinal microorganisms is researched from a comprehensive perspective, and the potential correlation is mined. The application of the method not only is expected to deepen the understanding of disease mechanisms, but also provides a powerful tool for future medical research, and promotes the development of personalized medical treatment and treatment strategies. The problem that no related technology for extracting the relation of the microbial diseases exists at present is solved.

Antimicrobial compositions include a therapeutically effective amount of neem oil, extract of the plant Hypericum spp., polyunsaturated fatty acids (PUFAs), carrageenans, and / or chlorhexidine; and (optionally) in combination with one or more pharmaceutically acceptable carriers, additives, and / or diluents. The antimicrobial compositions are effective in treating or preventing upper respiratory tract infections, preferably upper respiratory tract infections caused by human Coronaviruses and are included within methods of treating or preventing infection of the upper respiratory tract of humans by a microbial pathogen.

Provided herein are compounds of general Formula I:or agriculturally acceptable salts thereof. The compounds of Formula I can be used to improve the health of plants. For example, the compounds of Formula I can be used to inhibit a microbial pathogen of a plant, or to increase resistance of a plant to one or more abiotic stress. Methods of manufacturing the compounds of general Formula I, as well as synthetic intermediates are also provided.

The present invention aims to provide a novel triterpenoid saponin vaccine adjuvant. By means of molecular design, synthesis, activity evaluation, and other means, the adjuvant significantly improves immunostimulatory activity thereof while reducing the potential toxicity thereof and significantly enhancing the stability of a product. Specifically, the adjuvant of the present invention can induce a Th1 / Th2 mixed immune response, effectively improve the ability of an antigen presenting cell (such as a dendritic cell) to uptake, treat, and present an antigen (including but not limited to a microbial pathogen, a cancer cell, etc.), thereby enhancing the cellular immune efficacy and exhibiting great potential for preventing and treating a disease caused by a related pathogen.

Compositions and methods are described that provide safe and effective prophylactic treatment of a wide range of microbial pathogens. Such compositions and methods incorporate theta defensins and theta defensin analogs, which have been found to have broad-spectrum antibacterial effects, maintain effective concentrations in the bloodstream for extended periods of time, and are safe to administer at high doses.

The utility model relates to the technical field of virus inspection and inactivation, and discloses a microbial virus inspection and inactivation device which comprises a virus inactivation box shell, an inner cavity of the virus inactivation box shell is provided with an inactivation bin; the sealing plate is arranged above the inactivation bin, a motor is mounted on the upper surface of the sealing plate, a rotor of the motor penetrates through the surface of the sealing plate and is coaxially provided with a rotating rod, and stirring rods are uniformly mounted on the surface of the rotating rod; the heater is arranged on the lower surface of the top plate of the virus inactivation box shell, and gas conveying pipes are mounted on the two sides of the bottom of the heater. A stirring rod rotates to stir microbial virus liquid in the inactivation bin, so that the microbial virus liquid can flow and can be in uniform contact with hot air, and a heating plate can uniformly inject the hot air into the microbial virus liquid through an air outlet pipe; the microbial viruses can be effectively subjected to high-temperature inactivation treatment.

A grade of a specific microbial disease in a biological sample of a subject is determined. In one example, masked microbial reference genomes are used to determine the amount of free DNA molecules corresponding to a particular microbial species associated with a particular microbial disease. The masking may remove an area shared with another. In another example technique, end motifs are used for free DNA fragments from a subject and from a particular microbial species. A correlation between a subject and an amount of a set of terminal sequence motifs of a particular microbial species may be determined. The two groups of quantities of positive subjects are substantially more correlated than negative subjects.