35 results about "Tegillarca granosa" patented technology

The invention discloses a processing technology for purifying pollutants in four kinds of hard-shelled seawater shellfish by adopting an ultraviolet seawater treatment technique, wherein the four kinds of hard-shelled seawater shellfish include Ruditapes philippinarum, Meretrix meretrix Linnaeus, Cyclina sinensis and Tegillarca granosa. The technology provided by the invention adopts a semi-closed circulating system and the processing is conducted in a room with environmental temperature capable of being regulated. The technology is implemented through the following six steps of: selecting shellfish, cleaning the shellfish, containing the shellfish in a basket and placing the basket into a purifying pond; pumping deep-well seawater with seawater quality which satisfies the requirement into a circulating water pond; pumping the deep-well seawater into an ultraviolet sterilizer for sterilization through a water pump; spraying the sterilized seawater into the purifying pond to purify the shellfish according to technological parameters; draining return water into the circulating water pond for recirculation; and completing the purification technological process of one batch till the hygienic quality standard on the shellfish is met or after the minimum purification time which is 24 hours. The processing technology has the advantages that the pollutant removing effect is remarkable, the vitality of the shellfish after purification is strong, the technology is simple, the investment is small and the like.

The invention discloses an anti-prostate-cancer tegillarca granosa protein polypeptide as well as a preparation method and application thereof. The amino acid sequence of the anti-prostate-cancer tegillarca granosa protein polypeptide is Pro-Gln-Trp-Pro-Pro (PEWPP), the ESI/MS (electron spray ionization/mass spectrometry) detection shows that the molecular weight of the anti-prostate cancer tegillarca granosa protein polypeptide is 624.65Da. The preparation method of the anti-prostate-cancer tegillarca granosa protein polypeptide comprises the steps of: mincing tissues, hydrolyzing with an enzyme, ultra-filtrating and chromatographing. The prepared high-activity anti-prostate-cancer tegillarca granosa protein polypeptide with the amino acid sequence of Pro-Gln-Trp-Pro-Pro (PEWPP) has a significant effect of inhibiting the proliferation of human prostate cancer cells PC-3 and can be used as an anti-prostate cancer medicine or health food.

The invention relates to a method for increasing a culture yield of mudflat shellfish by selectively culturing a pond microalgae community. The method is particularly characterized in that for culture of pond tegillarca granosa and anadara subcrenata, the microalgae community in a pond is selectively cultured to be brown yellow by adjusting water body nutritive salt composition, and for clam and sinonovacula constricta, the microalgae community in the pond is selectively cultured to be yellowgreen by adjusting the water body nutritive salt composition. According to the method, a growth rate of the corresponding mudflat shellfish is obviously higher than that of the mudflat shellfish by the traditional culture, and the culture yield of the corresponding mudflat shellfish in a unit area of the pond can be increased greatly.

The invention provides a processing method for instant shelled seasoning tegillarca granosa. The processing method comprises the steps of cleaning the tegillarca granosa to remove silt, and picking out dead tegillarca granosa; putting the remained tegillarca granosa into a purification basin, adding purification liquid, covering the purification basin with ice blocks for purification for 24-28 hours, and washing the tegillarca granosa which is subjected to the purification through purified water, wherein the mass percentage of seawater crystals in the purification liquid is 3-5 percent, and the concentration of chlorine dioxide in the purification liquid is 2-4mg/L; draining the water from the tegillarca granosa on a stainless steel meshed disk; then adding sauces for seasoning, and draining the water from the tegillarca granosa on the stainless steel meshed disk after the tegillarca granosa is seasoned; putting the tegillarca granosa into a vacujm packaging machine according to an instant amount for vacuumizing packaging; performing ultrahigh-pressure treatment, wherein setting parameters during the ultrahigh-pressure treatment are that the pressure is 300-350MPa, the pressure retaining time is 5-10min, the temperature is 1-4 DEG C, and an ultrahigh-pressure cavity solvent is the purified water; finally putting the tegillarca granosa into a refrigerator with the temperature of 1-4 DEG C for refrigeration. The processing method is good in sterilization effect, and the taste of a product can be improved; furthermore, a customer can directly eat the product conveniently.

The invention discloses shellfish heparin with a mild anti-coagulation effect as well as a preparation method and the application thereof. The preparation method comprises the following steps: raw material treatment; self-dissolution; enzymolysis: adding NaOH to regulate the pH value to 7.8-8.2, adding trypsin, performing enzymolysis for 3.5-6.5 hours at a temperature of 32-42 DEG C, then adding HCl to regulate the pH value to be 6.8-7.2, adding papain and performing enzymolysis for 4.5-5.5 hours at a temperature of 62-68 DEG C to obtain an hydrolysate; enzyme inactivation and centrifugation;concentration and alcohol precipitation; re-dissolution and protein removal; de-salting and drying. The shellfish heparin as well as the preparation method and the application thereof disclosed by theinvention has the benefits that The shellfish heparin with the anti-coagulation effect is prepared by taking celomactra antiquata, panopea abrupta, tapes dorsatus, tegillarca granosa and meretrix assources for the first time; the heparin comes from marine shellfishes and is high in safety and mild in effect; not only are the sources of the heparin widened, but also the preparation method is simple, the product quality is stable, and application values are good in the preparation of the heparin with low side effects or other products with mild anti-coagulation effects.

The invention relates to a method for adsorbing cadmium exceeding standard in seawater through cultivation of Ttgillarca granosa and belongs to the field of marine environmental protection; when the content of cadmium in the seawater exceeds the standard and is within the range of between 5.1 and 50 mu g/L, the water quality of the seawater is in the third class, fourth class and inferior fourth class and is unsuitable for cultivation and production; the experiment proves that when Ttgillarca granosa is cultivated in the seawater polluted by cadmium, the age of the Ttgillarca granosa is between 3 and 4 years; the weight is between 4.6 and 7.7 g; the Ttgillarca granosa is grown into formed Ttgillarca granosa with shell width of between 2.13 and 2.78 cm and shell length of between 1.61 and 2.04 cm; the cultivation density is not less than 160 pieces in each square meter (calculated according to average water depth of 0.2 meter); cadmium exceeding the standard in the seawater can be effectively adsorbed; the seawater is restored to the standard of second-class water quality, that is cadmium in the seawater is less than or equal to 5mu g/L; and the Ttgillarca granosa also has function of releasing cadmium and can come into the market as a commodity. The biological method for adsorbing cadmium has effects of convenience and low consumption and has economic, social and ecological benefits.

The invention aims at providing a Tegillarca granosa antioxidant protein Peroxiredoxin-6 (Prx6) having an amino acid sequence of SEQ ID NO: 1. According to the invention, by virtue of screening and cloning the Tegillarca granosa antioxidant protein Prx6 gene, the full-length sequence of the Prx6 gene is obtained, and by virtue of bioinformatic analysis on the full-length sequence, the expression mechanism and control and regulation mechanism of the Prx6 gene are further explored and the contents of research on Tegillarca granosa immunology and molecular genetics are enriched. The invention provides a theoretical basis for subsequent research on the functional genes and molecular breeding and for discovery of candidate genes related to the major economic traits such as growth, reproduction, nutrition, metabolism and disease resistance.

The invention provides a preparation method of a novel non-natural antioxidation peptide and belongs to the field of biological medicine. The synthesis method of the novel polypeptide comprises the steps of performing resin grafting reaction on Fmoc-Pro(Boc)-OH, and then performing coupled reaction with Pro or Trp amino acid and taurine to form the novel polypeptide such as Tau-Trp-Pro, Trp-Tau-Pro, Tau-Trp-Pro-Pro, Trp-Pro-Tau-Pro and Trp-Tau-Pro-Pro, wherein a Fmoc removal group adopts a deprotection agent combination: piperidine, potassium tert-butoxide and HOBT in a reaction process. Taurine reacts with a tegillarca granosa tripeptide Trp-Pro-Pro to form the novel polypeptide with improved antioxidation activity or anticancer activity; in a synthesis process of the polypeptide, amino deprotection is complete; and the synthesis purity of the polypeptide is high.

The invention provides application of Tegillarca granosa hemoglobin antimicrobial peptide in food preservation and fresh-keeping, An amino acid sequence of the Tegillarca granosa hemoglobin antimicrobial peptide is shown as SEQ ID NO:1. The Tegillarca granosa hemoglobin antimicrobial peptide has a high antibacterial effect on Listeria monocytogenes, can be adsorbed on the cell membrane surface ofListeria monocytogenes to destroy the cell membrane to cause the death of Listeria monocytogenes, the permeability of the cell membrane of Listeria monocytogenes can be changed, and the cell membraneformation is inhibited. Experimental basis is provided for finding new food preservatives, and the healthy and sustainable development of national food industry are promoted.

The invention relates to a method and a processing device for breeding young tegillarca granosa, wherein a breeding chamber and regulation and control conditions of physicochemical environment in an algae workshop are involved; the breeding steps are as follows: (1) inducing spawning, (2) washing eggs and incubating, (3) selecting excellent ones, (4) breeding larvae, (5) preprocessing an attachment substrate, (6) collecting and distributing in a pool, and (7) breeding juvenile mollusks. The method is characterized in that the step (5) of preprocessing the attachment substrate comprises screening and washing the collected beach bottom mud by using a 150-200 mesh screen, rinsing out organic matters after precipitation, and then directly putting the beach bottom mud in a container barrel for boiling by introducing boiler steam into the beach bottom mud, and then cooling before distributing in the pool. The device is characterized in that a steam pipe extending out of a boiler extends into the container barrel and the lower mouth of the steam pipe is located under the mud surface of the beach bottom mud. The method and the processing device have the advantages that the beach bottom mud is boiled by steam so that heating is even and no hardening and coking are caused, the nutritional ingredients of the attachment substrate are kept, and drugs such as antibiotics, chloromycetin, potassium permanganate and the like are not used for sterilization, and the survival rate of breeding is high. After the method and the processing device provided by the invention are applied, the survival rate is increased to the range from 60% to 80%; and the yield of the young tegillarca granosa per square meter per batch reaches up to 0.35 kg.

The invention provides a method for improving gonad development of tegillarca granosa. The gonad development of the tegillarca granosa is improved by feeding thraustochytrium roseum during cultivation of parent scallops of the tegillarca granosa. The gonad development of the tegillarca granosa is greatly fastened by feeding the thraustochytrium roseum in cultivation of the parent scallops of the tegillarca granosa, egg-laying time of the parent scallops of the tegillarca granosa is effectively controlled, management of breeding production of seedlings of the tegillarca granosa is greatly facilitated, and ovulation rate of the parent scallops of the tegillarca granosa is greatly increased.

The invention discloses a processing technology for purifying pollutants in four hard-shelled seawater shellfishes like Ruditapes philippinarum, Meretrix meretrix, Cyclina sinensis and Tegillarca granosa through an ultraviolet seawater treatment technology. The technology provided by the invention comprises a semi-closed circulation system, and is performed in a temperature-adjustable indoor environment. The technology is implemented through the following six steps: subjecting shellfish to picking, performing cleaning and basketing, then placing the basketed shellfish in a purifying pond, pumping deep-well seawater of which water quality meets the requirements into a circulation water pond, then transporting the seawater into an ultraviolet sterilizer through a water pump and carrying out sterilization, ejecting the sterilized seawater into the purifying pond according to process parameters to purify the shellfish, then discharging return water into the circulation water pond again, and carrying out repeated circulation until the shellfish product hygienic quality standard or purification with a minimum time of 24 hours is achieved so as to complete a batch of a purification processing process. The processing technology provided by the invention has the advantages of significant pollutant removal effects, strong vitality of purified shellfish, simple process, low investment, etc.

The invention discloses a vibration screening and cleaning machine for tegillarca granosa. According to the technical scheme, the vibration screening and cleaning machine comprises a fixed base and a screening and cleaning mechanism matched with the tegillarca granosa, wherein the screening and cleaning mechanism comprises two matched screening side plates, and an upper screening plate, a lower screening plate and a flow guide structure are sequentially connected between the two screening side plates from top to bottom; a flow guide cavity matched with the upper screening plate and the lower screening plate is formed in the flow guide structure, and a flow guide opening is formed in the lower end of the flow guide cavity; a plurality of evenly-distributed flushing spraying pipes are fixedly connected between the two screening side plates, and evenly-distributed water spraying holes are formed in the lower portions of the flushing spraying pipes; a plurality of flushing spray heads matched with the flushing spraying pipes are arranged on the screening side plates; and the screening and cleaning mechanism further comprises a vibration device matched with the upper screening plate and the lower screening plate. The vibration screening and cleaning machine has the effects of being simple in structure, low in labor intensity and capable of rapidly screening tegillarca granosa of different sizes and effectively cleaning the tegillarca granosa.

The invention relates to an antibacterial peptide, in particular to a tegillarca granosa hemoglobin alpha-helix antibacterial peptide and application thereof. The antibacterial peptide comprises an amino acid sequence selected from SEQ ID NO: 1 or consists of the amino acid sequence, has an effective antibacterial effect, and enhances the antibacterial activity of the antibacterial peptide on bacteria through low-intensity ultrasonic processing. The TGH2 subjected to low-intensity ultrasonic-assisted treatment has a strong inhibition effect on bacteria, and meanwhile, the alpha helical structure of the antibacterial peptide TGH2 is kept stable. The invention provides an experimental basis for a low-intensity ultrasonic sterilization method and TGH2 as a food preservative.

The invention discloses a method for measuring the fatness breeding value of tegillarca granosa. According to the technical scheme, the method is characterized by comprising the steps of 1, weighing tegillarca granosa; 2 carrying out a descaling operation on the balance; 3, measuring and calculating the buoyancy; 4, recording data; and 5, carrying out breeding calculation, namely, obtaining the volume Vn=V<discharge>=Fn/(rho*g) of the measured object according to the fact that the buoyancy borne by the object in water is equal to the volume of discharged water, and then performing calculationaccording to a breeding calculation formula, so that the fatness breeding value of tegillarca granosa is equal to (1.85-Mn/Vn)/0.4*100. According to the method, the fatness of tegillarca granosa is indirectly reflected by measuring the individual density of tegillarca granosa, the individual density is converted into the breeding value, and the character of the individual fatness is reflected through the breeding value. The method has the effects that the character breeding work of tegillarca granosa is facilitated, and the fatness is reflected without causing the death of tegillarca granosa cannot in the measuring process.