45 results about "Antioxidant protein" patented technology

The invention relates to a method of diagnosing non-alcoholic steatohepatitis (NASH) using molecular markers. The inventive method consists in detecting and quantifying, in vitro in a hepatic tissue sample, the levels of a protein which can be used as a NASH molecular marker and which is selected from apolipoprotein A1, sub-unit β of the mitochondrial ATPase, leukotriene A4 hydrolase, keratin 18, guanidine acetate N-methyltransferase, superoxide dismutase, albumin, antioxidant protein 2 (isoform 1), prohibitin 1, methionine adenosyl transferase, long-chain acyl CoA dehydrogenase, selenium binding protein, antioxidant protein 2 (isoform 2), and combinations of same. The invention further consists in comparing the results obtained with the normal values of said proteins in healthy hepatic tissue. Said method can be used to diagnose NASH and/or to assess a patient's potential risk of developing NASH.

The invention provides fish-skin protein peptides with an antioxidation function. The amino acid sequences of the fish-skin protein peptides are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2. Based on the characteristics of fish-skin proteins, fish-skin protein peptide powder is prepared from fish skin through ultrasonic pretreatment, high-temperature high-pressure pretreatment, step-by-step enzymatic hydrolysis via a plurality of proteases and separation technologies including membrane separation, gel separation and high-performance liquid chromatographic separation, and the fish-skin protein peptides as shown SEQ ID No. 1 and 2 account for 50% or more of the total weight of the fish-skin protein peptide powder; so a simple high-efficiency preparation method for the fish-skin protein peptides with the antioxidation function is established.

The present invention relates to a mitochondria targeting peptide, a fusion protein in which the peptide is bound to the carboxyl terminus of a protein transduction domain, a fusion protein in which the peptide is bound to the carboxyl terminus of a protein transduction domain and an antioxidant is bound to the carboxyl terminus of the peptide, an antioxidant composition and a composition for preventing or treating Parkinson's disease including the fusion protein in which the antioxidant is bound, a recombinant polynucleotide in which a polynucleotide coding a protein transduction domain, a polynucleotide coding the peptide, and a polynucleotide coding an antioxidant protein are sequentially bound, to an expression vector including the polynucleotide, and to a transformed host cell including the expression vector.

The mitochondria targeting peptide according to the present invention targets mitochondria with high efficiency not only when the peptide exists alone but also when the peptide is bound to a protein transduction domain and/or to an antioxidant. Further, the peptide has a small size and is thus a very suitable targeting carrier. The peptide becomes processed when introduced into mitochondria, and thus stably delivers the drug carried by the peptide.

The invention relates to a light aging resistant composition and application thereof to essence. A product uses glycogen, Ectoin, cluster pine bark extracts, beta-glucan and glycerinum as raw materials; proper weight proportioning is performed; various substances achieve a cooperated effect; the finally prepared light aging resistant composition can resist light pollution from the inside of the skin; the free radical formation is reduced; damaged cell DNA is restored; the formation of inflammation and red spots can be inhibited; the generation of cell ATP, hyaluronic acid, ceramide and antioxidation protein can be promoted; the skin light aging can be actively resisted. The obtained composition is safe, mild and inirritative, and is applicable to any kinds of skin. According to light agingmechanisms, the product aims at each link causing the skin damage, can safely, effectively and actively solve the light aging problem to restore the cell activity and to promote collagen synthesis, so that winkles are reduced. The composition has the advantages that the preparation method is simple; the addition into a recipe is easy; the light aging problem is effectively solved.

The invention discloses a cassava starch hydrogel nostoc commune extract mask matrix and a preparation method thereof. By adopting a modern polymer synthesis technology, a fine chemical technology and the principle of natural organic chemical extraction, ultrasonic dispersion is adopted to perform cassava starch gelatinization treatment, and then crosslinking modification is performed to prepare cassava starch hydrogel; antioxidative protein is extracted from nostoc commune by adopting natural organic extraction, extraction and other methods, is wrapped by the hydrogel by adopting an adsorption method and then is processed into the mask matrix having oxidation resistance, scavenging free radicals and having high water retention, water supplementing activities and the like. The cassava starch hydrogel nostoc commune extract mask matrix prepared by means of the preparation method is stable in performance, has the characteristics of high water retention and water binding capacity of the hydrogel, also contains organic active substances of a nostoc commune extract and has the multiple effects of oxidation resistance, tumor resistance, virus resistance, bacterial resistance and inflammatory resistance. In addition, the mask matrix is simple in preparation process, good in biocompatibility, easy to degrade and environmentally friendly.

The invention discloses a nucleotide sequence with the function of enhancing hypoxia tolerance of fish. The base sequence of the nucleotide sequence is disclosed as (a) or (b) or (c) or (d): (a) base sequence disclosed as SEQ ID NO:1; (b) base sequence disclosed as SEQ ID NO:2; (c) base sequence for coding the protein with the amino acid sequence disclosed as SEQ ID NO:3; or (d) nucleotide sequence for hybridizing with (a) or (b) defined nucleotide sequence under strict hybridization conditions and coding mercapto-specific oxidation-resistant protein. The invention also discloses application of the nucleotide sequence protein, recombinant vector or host cell in enhancing hypoxia tolerance of fish and breeding hypoxia-tolerance transgenic fish varieties. When being integrated into the receptor fish genome as a transplanting gene and effectively expressed, the fish hypoxia-tolerance gene can obviously enhance the hypoxia tolerance of the receptor fish cells and organisms.

The invention aims at providing a Tegillarca granosa antioxidant protein Peroxiredoxin-6 (Prx6) having an amino acid sequence of SEQ ID NO: 1. According to the invention, by virtue of screening and cloning the Tegillarca granosa antioxidant protein Prx6 gene, the full-length sequence of the Prx6 gene is obtained, and by virtue of bioinformatic analysis on the full-length sequence, the expression mechanism and control and regulation mechanism of the Prx6 gene are further explored and the contents of research on Tegillarca granosa immunology and molecular genetics are enriched. The invention provides a theoretical basis for subsequent research on the functional genes and molecular breeding and for discovery of candidate genes related to the major economic traits such as growth, reproduction, nutrition, metabolism and disease resistance.

The present invention discloses a manufacture method of curcumin-ovalbumin compound antioxidant protein drink. The manufacture method comprises the following steps: (1) ovalbumin powder is subjected to hydration to obtain an ovalbumin solution having a concentration of 4-8 mg/mL; and (2) the curcumin and ovalbumin are mixed at a volume ratio of 1:(2-6) to prepare a curcumin-ovalbumin compound solution. The ovalbumin is used as a carrier to embed the curcumin; bioavailability of lipid-soluble polyphenol curcumin is improved; the stable curcumin-ovalbumin compound solution is formed; and the manufacture method of the functional drink with a rich protein source is provided to satisfy consumers' pursuits of healthy, personalized and functional drink.

The invention relates to the technical field of gene engineering and biological enzyme with an aim to provides a preparation method and application of microbial-source antioxidant protease. The amino acid sequence of the protease is as indicated in SEQ ID NO.1. The invention further the preparation method of the protease and the application of the protease in preparing drugs or health food used for inhibiting body aging and cell apoptosis. The protease is the important protease for oxidoreduction balance adjustment and plays an important role in biological oxidative stress and damage repair process, thereby being potential in application value in the anti-aging field. Through combination and regulation of factors of ASK1, NF-k and the like, body ageing and cell apoptosis are inhibited, cells can be subjected to damage repair and proliferation, cell ageing and cell apoptosis can be postponed, and the protease is one of important targets in tumor and cancer treatment; the protease is soluble in physical characteristic, natural activity is retained, and wide application prospect is achieved.

The invention discloses a compound extract of rhizoma polygonati and Chinese wolfberry fruits, as well as a preparation method and application thereof. The compound extract is prepared from rhizoma polygonati and Chinese wolfberry fruit extract, wherein the weight ratio of rhizoma polygonati to Chinese wolfberry fruit raw materials is 1:5-5:1, and the crude herbal concentration of the extract is 0.1mg/mL-5mg/mL. The compound extract can be used for enhancing expression of peroxire-doxin in cells and/or ROS content in fibroblasts after UVA radiation and increasing the mitochondria activity of epidermal keratinocytes and/or the content of adenosine triphosphate in the epidermal keratinocytes. The compound extract is added into a skin externally applied preparation as a functional additive for skin care.

The invention provides a preparation method of porphyra yezoensis antioxidant protein peptide, comprising the following steps: carrying out crushing treatment, water extraction and sterilization on porphyra yezoensis which is used as a raw material so as to obtain a leaching liquor; inoculating lactic acid bacteria to the leaching liquor to carry out fermentation, and carrying out sterilization treatment on the mixed liquor after the fermentation; carrying out enzymolysis treatment on the sterilized mixed liquor by the use of papain; carrying out inactivation after enzymolysis, cooling, carrying out centrifugation and collecting a supernatant; filtering the collected supernatant and drying so as to obtain porphyra yezoensis antioxidant protein peptide. According to the preparation method of porphyra yezoensis antioxidant protein peptide, by combining lactic acid bacteria fermentation and enzymolysis, the peptide product which has no bitter taste but fermentation flavor and good palatability can be prepared; and the .OH free radical and DPPH. Free radical removal capacity of the prepared antioxidant protein peptide also can be greatly enhanced. The preparation method has positive influence of increasing added value of laver.

The present invention relates to a composition for combating oxidation and alleviating the detrimental effects on skin caused by Asian Dust, the composition containing Melia azedarach bark and Nelumbo nucifera leaf extracts as a novel skin irritation alleviating agent. More specifically, the present invention contains Melia azedarach bark and Nelumbo nucifera leaf extracts, and entails: detoxification and expulsion of toxins by increasing the expression of NQO1 which is an enzyme that detoxifies toxins and of GSTT1 which is an enzyme that expels toxins; protection of the skin by increasing the expression of the skin antimicrobial peptide hBD3 which is a natural immune factor; and suppression of the production of active oxygen by increasing expression of the antioxidant proteins GPX1, catalase, Trx1 and Prdx1. Consequently, the invention has the advantages of skin protection and ageing suppression.

The invention belongs to the technical field of animal husbandry and discloses a tea polyphenol milk cow feed, which comprises feed and a tea polyphenol additive, and the feed and the tea polyphenol additive are stirred and mixed. The invention provides a tea polyphenol dairy cow feed, wherein the tea polyphenol additive can activate the intracellular Nrf2-ARE pathway, enhance the expression of antioxidant proteins, improve the antioxidant capacity of cells, reduce cell damage and mutation, and significantly reduce the risk of oxidative stress. It is a new and effective method to treat oxidative stress-related diseases in dairy cows by reducing the incidence of stress-related metabolic diseases in dairy cows and greatly improving the production performance and health status of dairy cows.

The present invention relates to new microorganism strains L. plantarum MCC1 DSM 23881 and L. gasseri MCC2 DSM 23882 and their use as antioxidant proteolytic ingredients for the production of food products and dietary supplements. Food products and dietary supplements containing L. plantarum MCC1 and L. gasseri MCC2 are hypoallergenic, reduce milk allergy and lower urinary tract irritation symptoms accompanying benign prostatic hyperplasia and oxidative stress and inflammation associated with these.

The invention discloses a preparation method of a piglet intestinal mucosa protective agent. The method includes preparing a fecal bacteria suspension by using fresh pig feces, preparing a zinc-loadedchitosan suspension, slowly adding the zinc-loaded chitosan suspension into the fecal bacteria suspension under stirring, and placing the mixture on ice for reaction for 3-8 minutes under the condition of continuous stirring; and adjusting the pH value to 7.0-7.4 after the reaction is finished to obtain the piglet intestinal mucosa protective agent, wherein the volume ratio of the fecal bacteriasuspension to the zinc-loaded chitosan suspension is 98: 2-99.5: 0.5. The piglet intestinal mucosa protective agent can be used as oral liquid for piglet, can enhance protective autophagy of piglet intestinal mucosa, improve antioxidant protein level, reduce inflammatory reaction, improve intestinal mucosa function, and also have zinc supplement and diarrhea prevention effects.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a compound for treating acute lung injury and a preparation method and application thereof. The drug crystal is prepared by wrapping an anti-inflammatory drug with cationized globulin, and the crystal is positively charged under physiological conditions. The antioxidant protein is acidic protein, is negatively charged under physiological conditions, and can be combined with the drug crystal through electrostatic interaction to form a compound. The prepared compound can be efficiently targeted to endothelial cells, small molecule drugs and protein drugs are simultaneously delivered into the vascular endothelial cells in a non-lysosome way, the effect of asynchronous slow-control drug release is achieved, and the drugs are specifically accumulated in the lung, so that the vascular endothelial cells are better repaired, and inflammation is relieved.

Oligodendrocytes (OLs), the predominant cell type found in cerebral white matter, are essential for structural integrity and proper neural signaling. Very little is known concerning stroke-induced OL dysfunction. Infusion of human umbilical cord blood (HUCB) cells protects striatal white matter tracts in vivo and directly protects mature primary OL cultures from oxygen glucose deprivation (OGD). Microarray studies of RNA prepared from OL cultures subjected to OGD and treated with HUCB cells showed an increase in the expression of 33 genes associated with OL proliferation, survival, and repair functions, such as myelination. Immunohistochemistry showed antioxidant protein expression was upregluated in the ipsilateral white matter tracts of rats infused with HUCB cells 48 hrs after middle cerebral artery occlusion (MCAO). These results show expression of genes induced by HUCB cell therapy that could confer oligoprotection from ischemia.