56 results about "Antioxidant protein" patented technology

The invention relates to a method of diagnosing non-alcoholic steatohepatitis (NASH) using molecular markers. The inventive method consists in detecting and quantifying, in vitro in a hepatic tissue sample, the levels of a protein which can be used as a NASH molecular marker and which is selected from apolipoprotein A1, sub-unit β of the mitochondrial ATPase, leukotriene A4 hydrolase, keratin 18, guanidine acetate N-methyltransferase, superoxide dismutase, albumin, antioxidant protein 2 (isoform 1), prohibitin 1, methionine adenosyl transferase, long-chain acyl CoA dehydrogenase, selenium binding protein, antioxidant protein 2 (isoform 2), and combinations of same. The invention further consists in comparing the results obtained with the normal values of said proteins in healthy hepatic tissue. Said method can be used to diagnose NASH and / or to assess a patient's potential risk of developing NASH.

The invention provides fish-skin protein peptides with an antioxidation function. The amino acid sequences of the fish-skin protein peptides are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2. Based on the characteristics of fish-skin proteins, fish-skin protein peptide powder is prepared from fish skin through ultrasonic pretreatment, high-temperature high-pressure pretreatment, step-by-step enzymatic hydrolysis via a plurality of proteases and separation technologies including membrane separation, gel separation and high-performance liquid chromatographic separation, and the fish-skin protein peptides as shown SEQ ID No. 1 and 2 account for 50% or more of the total weight of the fish-skin protein peptide powder; so a simple high-efficiency preparation method for the fish-skin protein peptides with the antioxidation function is established.

The invention relates to a method of diagnosing non-alcoholic steatohepatitis (NASH) using molecular markers. The inventive method consists in detecting and quantifying, in vitro in a hepatic tissue sample, the levels of a protein which can be used as a NASH molecular marker and which is selected from apolipoprotein A1, sub-unit β of the mitochondrial ATPase, leukotriene A4 hydrolase, keratin 18, guanidine acetate N-methyltransferase, superoxide dismutase, albumin, antioxidant protein 2 (isoform 1), prohibitin 1, methionine adenosyl transferase, long-chain acyl CoA dehydrogenase, selenium binding protein, antioxidant protein 2 (isoform 2), and combinations of same. The invention further consists in comparing the results obtained with the normal values of said proteins in healthy hepatic tissue. Said method can be used to diagnose NASH and / or to assess a patient's potential risk of developing NASH.

The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of: a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support, b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I, R—O—O—H (I) wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.

The present invention relates to a mitochondria targeting peptide, a fusion protein in which the peptide is bound to the carboxyl terminus of a protein transduction domain, a fusion protein in which the peptide is bound to the carboxyl terminus of a protein transduction domain and an antioxidant is bound to the carboxyl terminus of the peptide, an antioxidant composition and a composition for preventing or treating Parkinson's disease including the fusion protein in which the antioxidant is bound, a recombinant polynucleotide in which a polynucleotide coding a protein transduction domain, a polynucleotide coding the peptide, and a polynucleotide coding an antioxidant protein are sequentially bound, to an expression vector including the polynucleotide, and to a transformed host cell including the expression vector.The mitochondria targeting peptide according to the present invention targets mitochondria with high efficiency not only when the peptide exists alone but also when the peptide is bound to a protein transduction domain and / or to an antioxidant. Further, the peptide has a small size and is thus a very suitable targeting carrier. The peptide becomes processed when introduced into mitochondria, and thus stably delivers the drug carried by the peptide.

The invention discloses composite antioxidation peptides and a preparation method thereof. The fermentation substrates of the composite antioxidation peptides comprise the following raw materials in parts by weight: zein, soybean hydrolyzed protein and malt protein. According to the composite antioxidation peptides and the preparation method thereof, a bacillus subtilis seed bacterium suspension is used as a zymogen, the mixed dispersion system of the zein, the soybean hydrolyzed protein and the malt protein is used as a fermentation substrate, liquid state fermentation is performed, and a bacillus subtilis seed fermentation method and an enzymatic hydrolysis substrate are combined, so that a large number of functional antioxidation protein peptides can be decomposed and modified in the fermentation process. The composite antioxidation peptides and the preparation method thereof disclosed by the invention have the advantages that the technological processes are simplified, the preparation cost is reduced, and the products are diversiform. The prepared composite antioxidation peptides also have the advantages of being prominent in functions, high in rapid dissolving property, notable in processing characteristics, and the like, are suitable for the development of functional protein peptides having the effects of reducing blood pressure, resisting weariness, neutralizing the effect of alcoholic drinks, resisting oxidation and the like. According to the composite antioxidation peptides and the preparation method thereof disclosed by the invention, alcohol soluble protein is modified by using a fermentation method, so that the research stage is a climax period, and the problem that the functions of the single antioxidation peptide are deficient is solved.

The invention relates to a light aging resistant composition and application thereof to essence. A product uses glycogen, Ectoin, cluster pine bark extracts, beta-glucan and glycerinum as raw materials; proper weight proportioning is performed; various substances achieve a cooperated effect; the finally prepared light aging resistant composition can resist light pollution from the inside of the skin; the free radical formation is reduced; damaged cell DNA is restored; the formation of inflammation and red spots can be inhibited; the generation of cell ATP, hyaluronic acid, ceramide and antioxidation protein can be promoted; the skin light aging can be actively resisted. The obtained composition is safe, mild and inirritative, and is applicable to any kinds of skin. According to light agingmechanisms, the product aims at each link causing the skin damage, can safely, effectively and actively solve the light aging problem to restore the cell activity and to promote collagen synthesis, so that winkles are reduced. The composition has the advantages that the preparation method is simple; the addition into a recipe is easy; the light aging problem is effectively solved.

The invention belongs to the field of bioscience, and particularly relates to transmembrane Dps (starvation-induced DNA binding protein) and an application. By means of rational design of a protein nanocage derived from prokaryotes, the protein nanocage is enabled to be an antioxidant protein nanomaterial which can be applied to mammalian cells. The protein nanomaterial is characterized in that transportation across mammalian cell membranes of Dps is realized by fused expression of His-tag and Avitag (LNDIFEAQKIEWHE) at the N-terminal of Dps; the cells can be protected from being damaged by ROS (Reactive Oxygen Species); meanwhile, the protein nanomaterial is good in biocompatibility, simple to design, easy to prepare, high in yield and suitable for large-scale production.

A method of providing zinc to a subject in need of treatment includes administering to the subject an effective amount of a zinc-cysteine complex. The zinc-cysteine complex is preferably a zinc-monocysteine complex. The complexes of zinc-cysteine of the present invention increase the activity of antioxidant enzymes catalase and glutathione peroxidase, and the antioxidant protein metallothionein. The complexes of zinc-cysteine of the present invention protect retinal pigment epithelial cells from increasing hydrogen peroxide concentrations.

The invention discloses a cassava starch hydrogel nostoc commune extract mask matrix and a preparation method thereof. By adopting a modern polymer synthesis technology, a fine chemical technology and the principle of natural organic chemical extraction, ultrasonic dispersion is adopted to perform cassava starch gelatinization treatment, and then crosslinking modification is performed to prepare cassava starch hydrogel; antioxidative protein is extracted from nostoc commune by adopting natural organic extraction, extraction and other methods, is wrapped by the hydrogel by adopting an adsorption method and then is processed into the mask matrix having oxidation resistance, scavenging free radicals and having high water retention, water supplementing activities and the like. The cassava starch hydrogel nostoc commune extract mask matrix prepared by means of the preparation method is stable in performance, has the characteristics of high water retention and water binding capacity of the hydrogel, also contains organic active substances of a nostoc commune extract and has the multiple effects of oxidation resistance, tumor resistance, virus resistance, bacterial resistance and inflammatory resistance. In addition, the mask matrix is simple in preparation process, good in biocompatibility, easy to degrade and environmentally friendly.

The invention discloses a nucleotide sequence with the function of enhancing hypoxia tolerance of fish. The base sequence of the nucleotide sequence is disclosed as (a) or (b) or (c) or (d): (a) base sequence disclosed as SEQ ID NO:1; (b) base sequence disclosed as SEQ ID NO:2; (c) base sequence for coding the protein with the amino acid sequence disclosed as SEQ ID NO:3; or (d) nucleotide sequence for hybridizing with (a) or (b) defined nucleotide sequence under strict hybridization conditions and coding mercapto-specific oxidation-resistant protein. The invention also discloses application of the nucleotide sequence protein, recombinant vector or host cell in enhancing hypoxia tolerance of fish and breeding hypoxia-tolerance transgenic fish varieties. When being integrated into the receptor fish genome as a transplanting gene and effectively expressed, the fish hypoxia-tolerance gene can obviously enhance the hypoxia tolerance of the receptor fish cells and organisms.

The invention relates to application of XCT790 in preparation of a medicine for treating calcified aortic valve diseases and a culture method for inhibiting valve stromal cells from differentiating into osteoblasts. In an osteogenesis-induced in-vitro calcification model, it is shown that XCT790 can inhibit valve interstitial cells from being converted into osteoblasts, and it is proved that the XCT790 plays a role in protection by up-regulating antioxidant protein Hmox1. The beneficial effects of the present invention are mainly reflected in that: application of XCT790 in preparation of the medicine for treating calcified aortic valve diseases is provided, and the method for remarkably improving in-vitro osteogenic differentiation of valve interstitial cells after XCT790 treatment is provided, so that a new medicine choice is provided for calcified aortic valve diseases.

The invention aims at providing a Tegillarca granosa antioxidant protein Peroxiredoxin-6 (Prx6) having an amino acid sequence of SEQ ID NO: 1. According to the invention, by virtue of screening and cloning the Tegillarca granosa antioxidant protein Prx6 gene, the full-length sequence of the Prx6 gene is obtained, and by virtue of bioinformatic analysis on the full-length sequence, the expression mechanism and control and regulation mechanism of the Prx6 gene are further explored and the contents of research on Tegillarca granosa immunology and molecular genetics are enriched. The invention provides a theoretical basis for subsequent research on the functional genes and molecular breeding and for discovery of candidate genes related to the major economic traits such as growth, reproduction, nutrition, metabolism and disease resistance.

The invention relates to a preparation method of a water-resistant and anti-oxidative protein-based adhesive and belongs to the technical field of wood adhesive preparation. The preparation method comprises steps as follows: firstly, metallothionein is extracted from the liver of a meat rabbit and mixed with isolated soybean protein for modification, and pre-modified isolated soybean protein is obtained; pine sawdust is taken as a raw material and firstly subjected to ethanol wet milling pretreatment, the pretreated pine sawdust and an organic carboxylic acid solution are blended and subjectedto high-temperature and high-pressure cooking, modified pine sawdust is obtained, finally, the modified pine sawdust is subjected to blending reaction with the pre-modified isolated soybean protein,tourmaline powder and other auxiliary materials, and the water-resistant and anti-oxidative protein-based adhesive is obtained. The prepared water-resistant and anti-oxidative protein-based adhesive is high in adhesion strength, good in water resistance and high in oxidation resistance, is not prone to cracking and falling and has broad application prospect.

The present invention discloses a manufacture method of curcumin-ovalbumin compound antioxidant protein drink. The manufacture method comprises the following steps: (1) ovalbumin powder is subjected to hydration to obtain an ovalbumin solution having a concentration of 4-8 mg / mL; and (2) the curcumin and ovalbumin are mixed at a volume ratio of 1:(2-6) to prepare a curcumin-ovalbumin compound solution. The ovalbumin is used as a carrier to embed the curcumin; bioavailability of lipid-soluble polyphenol curcumin is improved; the stable curcumin-ovalbumin compound solution is formed; and the manufacture method of the functional drink with a rich protein source is provided to satisfy consumers' pursuits of healthy, personalized and functional drink.

The invention relates to the technical field of gene engineering and biological enzyme with an aim to provides a preparation method and application of microbial-source antioxidant protease. The amino acid sequence of the protease is as indicated in SEQ ID NO.1. The invention further the preparation method of the protease and the application of the protease in preparing drugs or health food used for inhibiting body aging and cell apoptosis. The protease is the important protease for oxidoreduction balance adjustment and plays an important role in biological oxidative stress and damage repair process, thereby being potential in application value in the anti-aging field. Through combination and regulation of factors of ASK1, NF-k and the like, body ageing and cell apoptosis are inhibited, cells can be subjected to damage repair and proliferation, cell ageing and cell apoptosis can be postponed, and the protease is one of important targets in tumor and cancer treatment; the protease is soluble in physical characteristic, natural activity is retained, and wide application prospect is achieved.

The invention discloses a compound extract of rhizoma polygonati and Chinese wolfberry fruits, as well as a preparation method and application thereof. The compound extract is prepared from rhizoma polygonati and Chinese wolfberry fruit extract, wherein the weight ratio of rhizoma polygonati to Chinese wolfberry fruit raw materials is 1:5-5:1, and the crude herbal concentration of the extract is 0.1mg / mL-5mg / mL. The compound extract can be used for enhancing expression of peroxire-doxin in cells and / or ROS content in fibroblasts after UVA radiation and increasing the mitochondria activity of epidermal keratinocytes and / or the content of adenosine triphosphate in the epidermal keratinocytes. The compound extract is added into a skin externally applied preparation as a functional additive for skin care.

The invention belongs to the technical field of medicinal natural products, and particularly relates to application of mogroside IIE in preparation of antioxidant food, health care products or drugs.The invention provides the new application of the mogroside IIE and provides a new direction for development and utilization of corsvenor momordica fruits, meanwhile, as a food functional component, the mogroside IIE has smaller toxic and side effects, can be used for preparing antioxidant food, health care products and drugs, and has broad market prospects. The research of the invention finds that the mogroside IIE can clearly remove ROS in cell tissue and shows the antioxidant capacity of the mogroside IIE. The mogroside IIE can activate a Nrf2 / ARE signal path, activate downstream antioxidant proteins and the expression of phase II detoxification enzymes: Nrf2, HO-1 and NQO1. The mogroside IIE can also inhibit the activation of a PI3K / AKT1 signal path, thereby exerting the antioxidant effect of the mogroside IIE.

The invention discloses a folium cortex eucommiae lignan compound, a preparation method and application of the folium cortex eucommiae lignan compound in neuroprotection, the folium cortex eucommiae lignan compound generates antioxidant protein by regulating a PI3K / AKT / GSK3beta / Nrf2 signal channel, nerve cells are protected from being influenced by oxidative stress, and the chemical structural formula of the folium cortex eucommiae lignan compound and corresponding nuclear magnetic data are obtained. The compound is separated from folium cortex eucommiae. In the compounds, (+)-7-epi-pinoresin mr1 (1) is a new lignan separated from eucommia ulmoides, and (2) is a new lignan separated from eucommia ulmoides. Through determination of cell activity, the enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), the level of reactive oxygen species (ROS) and the level of lactic dehydrogenase (LDH), 6 compounds in the 12 lignans show good neuroprotective activity; wherein the compound 1, the compound 2 and the compound 4 have the best activity, and the protein expression quantity of antioxidant proteins HO-1, NQO-1 and Cat is increased by adjusting a PI3K / AKT / GSK-3beta / Nrf2 signal channel, so that the compound 1, the compound 2 and the compound 4 have a remarkable neuroprotective effect.

The invention provides a preparation method of porphyra yezoensis antioxidant protein peptide, comprising the following steps: carrying out crushing treatment, water extraction and sterilization on porphyra yezoensis which is used as a raw material so as to obtain a leaching liquor; inoculating lactic acid bacteria to the leaching liquor to carry out fermentation, and carrying out sterilization treatment on the mixed liquor after the fermentation; carrying out enzymolysis treatment on the sterilized mixed liquor by the use of papain; carrying out inactivation after enzymolysis, cooling, carrying out centrifugation and collecting a supernatant; filtering the collected supernatant and drying so as to obtain porphyra yezoensis antioxidant protein peptide. According to the preparation method of porphyra yezoensis antioxidant protein peptide, by combining lactic acid bacteria fermentation and enzymolysis, the peptide product which has no bitter taste but fermentation flavor and good palatability can be prepared; and the .OH free radical and DPPH. Free radical removal capacity of the prepared antioxidant protein peptide also can be greatly enhanced. The preparation method has positive influence of increasing added value of laver.

The present invention relates to a composition for combating oxidation and alleviating the detrimental effects on skin caused by Asian Dust, the composition containing Melia azedarach bark and Nelumbo nucifera leaf extracts as a novel skin irritation alleviating agent. More specifically, the present invention contains Melia azedarach bark and Nelumbo nucifera leaf extracts, and entails: detoxification and expulsion of toxins by increasing the expression of NQO1 which is an enzyme that detoxifies toxins and of GSTT1 which is an enzyme that expels toxins; protection of the skin by increasing the expression of the skin antimicrobial peptide hBD3 which is a natural immune factor; and suppression of the production of active oxygen by increasing expression of the antioxidant proteins GPX1, catalase, Trx1 and Prdx1. Consequently, the invention has the advantages of skin protection and ageing suppression.

The invention belongs to the technical field of animal husbandry and discloses a tea polyphenol milk cow feed, which comprises feed and a tea polyphenol additive, and the feed and the tea polyphenol additive are stirred and mixed. The invention provides a tea polyphenol dairy cow feed, wherein the tea polyphenol additive can activate the intracellular Nrf2-ARE pathway, enhance the expression of antioxidant proteins, improve the antioxidant capacity of cells, reduce cell damage and mutation, and significantly reduce the risk of oxidative stress. It is a new and effective method to treat oxidative stress-related diseases in dairy cows by reducing the incidence of stress-related metabolic diseases in dairy cows and greatly improving the production performance and health status of dairy cows.

The present invention relates to new microorganism strains L. plantarum MCC1 DSM 23881 and L. gasseri MCC2 DSM 23882 and their use as antioxidant proteolytic ingredients for the production of food products and dietary supplements. Food products and dietary supplements containing L. plantarum MCC1 and L. gasseri MCC2 are hypoallergenic, reduce milk allergy and lower urinary tract irritation symptoms accompanying benign prostatic hyperplasia and oxidative stress and inflammation associated with these.

The invention discloses a method for preparing antioxidant active polypeptide based on tyrosinase modified collagen, silk fibroin and mussel protein, and belongs to the technical field of bioengineering. According to the invention, high-activity tyrosinase protein is screened and prepared, and the prepared tyrosinase is used for dopa modification of antioxidant protein. The dopa modification degree of the antioxidant protein prepared by the method disclosed by the invention is remarkably improved, and the DPPH free radical scavenging power and superoxide anion free radical scavenging power of the antioxidant protein are enhanced. The method is suitable for various proteins such as collagen, silk fibroin and mussel protein, can be used in the fields of functional food, cosmetics, biological materials and the like, and has good adaptability and wide application prospects.

The invention discloses a preparation method of a piglet intestinal mucosa protective agent. The method includes preparing a fecal bacteria suspension by using fresh pig feces, preparing a zinc-loadedchitosan suspension, slowly adding the zinc-loaded chitosan suspension into the fecal bacteria suspension under stirring, and placing the mixture on ice for reaction for 3-8 minutes under the condition of continuous stirring; and adjusting the pH value to 7.0-7.4 after the reaction is finished to obtain the piglet intestinal mucosa protective agent, wherein the volume ratio of the fecal bacteriasuspension to the zinc-loaded chitosan suspension is 98: 2-99.5: 0.5. The piglet intestinal mucosa protective agent can be used as oral liquid for piglet, can enhance protective autophagy of piglet intestinal mucosa, improve antioxidant protein level, reduce inflammatory reaction, improve intestinal mucosa function, and also have zinc supplement and diarrhea prevention effects.

The invention relates to the field of biological fermentation, in particular to a method for efficiently preparing anti-aging ergothioneine through fermentation of compound bacteria combined with bolete and application thereof.The method comprises the steps that halophilic bacillus SC-1, halophilic archaea IR-2 and thermophilic casein dissolving bacteria PC-3 are mixed according to the volume ratio of 1: 1: 1, bolete and pleurotus citrinopileatus are used as raw materials, and a mixture is prepared through fermentation; fermenting for 120 hours in an optimized basic culture medium under the conditions that the temperature is 30 + / -0.5 DEG C, the speed is 150rpm + / -10rpm and the pH value is 5.8 + / -0.2; through a step-by-step process of purifying EGT by using D101 resin and purifying RNET-1 by using DEAE-52 resin, a double-active-component extract is synchronously obtained. Through the synergistic effect of three strains with complementary functions, an efficient system of raw material degradation, carbon source supply, double precursor synthesis and double active component accumulation is constructed, synchronous high yield of ergothioneine and novel antioxidant protein RNET-1 is realized for the first time, and the blank of single active component in the prior art is filled.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a compound for treating acute lung injury and a preparation method and application thereof. The drug crystal is prepared by wrapping an anti-inflammatory drug with cationized globulin, and the crystal is positively charged under physiological conditions. The antioxidant protein is acidic protein, is negatively charged under physiological conditions, and can be combined with the drug crystal through electrostatic interaction to form a compound. The prepared compound can be efficiently targeted to endothelial cells, small molecule drugs and protein drugs are simultaneously delivered into the vascular endothelial cells in a non-lysosome way, the effect of asynchronous slow-control drug release is achieved, and the drugs are specifically accumulated in the lung, so that the vascular endothelial cells are better repaired, and inflammation is relieved.

Oligodendrocytes (OLs), the predominant cell type found in cerebral white matter, are essential for structural integrity and proper neural signaling. Very little is known concerning stroke-induced OL dysfunction. Infusion of human umbilical cord blood (HUCB) cells protects striatal white matter tracts in vivo and directly protects mature primary OL cultures from oxygen glucose deprivation (OGD). Microarray studies of RNA prepared from OL cultures subjected to OGD and treated with HUCB cells showed an increase in the expression of 33 genes associated with OL proliferation, survival, and repair functions, such as myelination. Immunohistochemistry showed antioxidant protein expression was upregluated in the ipsilateral white matter tracts of rats infused with HUCB cells 48 hrs after middle cerebral artery occlusion (MCAO). These results show expression of genes induced by HUCB cell therapy that could confer oligoprotection from ischemia.

The invention discloses an optimal buffer solution extraction test method for optimizing germinated walnut seed protein by a response surface method.The method comprises the steps that after seeds germinate, the amino acid composition and content of the walnut seeds in different germination periods are measured, and the optimal germination time is determined; then measuring the protein content and the antioxidant activity; and optimizing the optimal extraction condition by using a response surface method. According to the method, the current situation of walnut protein resource utilization and bean and cereal seed germination application research are combined, the protein content and amino acid composition and content change of germinated walnut seeds are analyzed, and the optimal germination time node is determined. According to the method, germinated walnut kernels are used as materials, a response surface optimization protein extraction test is utilized, antioxidant activity evaluation is combined, and an extraction and separation process for the antioxidant protein of the germinated walnut kernels is constructed, so that antioxidant protein components with high additional value are prepared, and accurate preparation of specific functional protein of the germinated walnut kernels is facilitated; a process and a scheme are provided for high-value utilization of subsequent walnut protein resources in the fields of food, health care products, daily chemicals and the like.