Manufacture method of curcumin-ovalbumin compound antioxidant protein drink
A technology of antioxidant protein and ovalbumin is applied in the field of preparation of curcumin-ovalbumin compound antioxidant protein beverage, which can solve the problems of restricting the application of curcumin, reducing the absorption rate, complicated process, etc. Bioactivity, good color, improved solubility and bioavailability
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Embodiment 1
[0021] The present embodiment provides a kind of preparation method of curcumin-ovalbumin compound antioxidant protein beverage, comprises the following steps:
[0022] (1) Ovalbumin powder is hydrated to obtain an ovalbumin solution with a concentration of 4-8 mg / mL; (2) Curcumin and ovalbumin are mixed at a volume ratio of 1:2-6 to prepare curcumin-ovalbumin Composite solution, the specific steps are as follows,
[0023] (1) Hydration of ovalbumin: Dissolve 300mg, 400mg, 500mg, 600mg, 700mg, and 800mg ovalbumin powder in 100mL distilled water respectively, stir on a magnetic stirrer for 2h, and place it for 6h. completed in ℃ environment.
[0024] (2) Preparation of curcumin-ovalbumin complex solution: adjust the pH of 50mL ovalbumin solution to 12 by using the pH shift method. Add 0.2mg / mL curcumin, and then add an appropriate amount of food flavor until there is no irritating smell. After reacting for 30 minutes, adjust the pH of the compound solution to a neutral pH of ...
Embodiment 2
[0027] Embodiment 2: investigate the impact of different concentrations of curcumin on the coating rate of ovalbumin
[0028] Investigate the impact of different concentration curcumin on the coating rate of ovalbumin on the basis of embodiment 1, concrete steps are as follows:
[0029] (1) Hydration of ovalbumin: Dissolve 500 mg of ovalbumin powder in 100 mL of distilled water, stir on a magnetic stirrer for 2 hours, and place for 6 hours. This step is completed at 1-5°C.
[0030] (2) Preparation of curcumin-ovalbumin complex solution: Using the pH shift method, adjust the pH of the completely dissolved 50mL ovalbumin solution to 12, keep it for 30min, curcumin and ovalbumin at a volume ratio of 1:4 Add different concentrations of curcumin (0.2, 0.4, 0.8, 1.6, 3.2mg / mL), and then add an appropriate amount of edible essence until there is no irritating smell. After reacting for 30 minutes, adjust the pH of the composite solution to a neutral environment pH of 7, and get Curcu...
Embodiment 3
[0034] On the basis of Examples 1 and 2, it was investigated whether the activity of curcumin changed after the compounding of curcumin and ovalbumin.
[0035] (1) Hydration of ovalbumin: Dissolve 500 mg of ovalbumin powder in 100 mL of distilled water, stir on a magnetic stirrer for 2 hours, and place for 6 hours. This step is completed at 1-5°C.
[0036] (2) Preparation of curcumin-ovalbumin composite solution: use the pH shift method to adjust the pH of the completely dissolved 50mL ovalbumin solution to 12, keep it for 30min, add curcumin and ovalbumin at a volume ratio of 1:4 0.2mg / mL curcumin, then add an appropriate amount of edible essence until there is no irritating smell, react for 30min, then adjust the pH of the compound solution to a neutral pH of 7 to obtain a curcumin-ovalbumin compound solution, which is pasteurized , heat the curcumin-ovalbumin complex solution to 68-70°C, keep this temperature for 30 minutes, and then rapidly cool it to 4-5°C, which is the c...
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