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Composite antioxidation peptides and preparation method thereof

An anti-oxidative peptide and composite technology, which is applied to the functions of food ingredients, food science, food ingredients, etc., can solve the problems of insufficient functionality of antioxidant peptides, and achieve the goal of solving functional insufficiencies, reducing preparation costs, and outstanding functions Effect

Inactive Publication Date: 2016-03-02
厦门赋源康实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Another object of the present invention is to solve the problem of insufficient functionality of a single species of antioxidant peptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 prepares composite antioxidant peptide of the present invention according to the following steps:

[0026] 1. Preparation of zein: Take 500g of corn gluten powder, the main by-product of corn starch produced by wet method, which requires a protein content of more than 70%, and undergo a supercritical decolorization process with carbon dioxide. The reaction temperature is 45°C, the outlet valve temperature is 100°C, and the reaction pressure is 400bar, extraction time 120min, fluid flow rate 6L / h for decolorization and impurity removal. According to microwave-ultrasonic combined leaching, add 70% ethanol 10000mL to the decolorized corn gluten powder, extract three times with a microwave reactor with 440W output power, each time for 60s, and centrifuge at 4000r / min for 30min to take the supernatant for later use; Add 5000mL of 70% ethanol to the protein powder after the supernatant, re-extract with ultrasonic wave, the extraction temperature is 45°C, ultrason...

Embodiment 2

[0030] Embodiment 2 prepares the composite antioxidant peptide of the present invention according to the following steps:

[0031] 1. Preparation of zein: take 500g of corn gluten powder, the main by-product of corn starch produced by wet method, which requires a protein content of more than 70%, and undergo a supercritical decolorization process with carbon dioxide. The reaction temperature is 50°C, the outlet valve temperature is 100°C, and the reaction pressure is 300bar, extraction time 120min, fluid flow rate 6L / h for decolorization and impurity removal. According to microwave-ultrasonic combined leaching, add 70% ethanol 10000mL to the decolorized corn gluten powder, extract three times with a microwave reactor with 440W output power, each time for 60s, and centrifuge at 4000r / min for 30min to take the supernatant for later use; Add 5000mL of 70% ethanol to the protein powder after the supernatant, re-extract with ultrasonic wave, the extraction temperature is 50°C, ultr...

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Abstract

The invention discloses composite antioxidation peptides and a preparation method thereof. The fermentation substrates of the composite antioxidation peptides comprise the following raw materials in parts by weight: zein, soybean hydrolyzed protein and malt protein. According to the composite antioxidation peptides and the preparation method thereof, a bacillus subtilis seed bacterium suspension is used as a zymogen, the mixed dispersion system of the zein, the soybean hydrolyzed protein and the malt protein is used as a fermentation substrate, liquid state fermentation is performed, and a bacillus subtilis seed fermentation method and an enzymatic hydrolysis substrate are combined, so that a large number of functional antioxidation protein peptides can be decomposed and modified in the fermentation process. The composite antioxidation peptides and the preparation method thereof disclosed by the invention have the advantages that the technological processes are simplified, the preparation cost is reduced, and the products are diversiform. The prepared composite antioxidation peptides also have the advantages of being prominent in functions, high in rapid dissolving property, notable in processing characteristics, and the like, are suitable for the development of functional protein peptides having the effects of reducing blood pressure, resisting weariness, neutralizing the effect of alcoholic drinks, resisting oxidation and the like. According to the composite antioxidation peptides and the preparation method thereof disclosed by the invention, alcohol soluble protein is modified by using a fermentation method, so that the research stage is a climax period, and the problem that the functions of the single antioxidation peptide are deficient is solved.

Description

technical field [0001] The invention relates to the technical field of functional food processing, in particular to a composite antioxidant peptide and a preparation method thereof. Background technique [0002] Zein is the main protein in corn. It has unique solubility properties, heat resistance, film-forming properties and anti-oxidation properties. It can be used for moisture-proof and oxygen-proof, anti-ultraviolet, fresh-keeping, anti-static, etc., and has certain antibacterial properties. Function, it contains a high proportion of hydrophobic aliphatic amino acids such as leucine, alanine and isoleucine, and there are functional sequence regions in its polypeptide chain such as anti-oxidation, anti-hypertension, and promoting alcohol metabolism. The modification method can release its functional properties for the development of functional foods. [0003] Soy protein is a nutritious high-quality plant protein, mainly composed of β-conglycinin (7S) and globulin (11S)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L33/18
CPCA23V2002/00A23V2200/326A23V2200/30A23V2250/55
Inventor 关海宁赵凤影刁小琴乔秀丽于新月张波张菊灵张子艳李杨王鹏
Owner 厦门赋源康实业有限公司
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