A compound antioxidant peptide and its preparation method
An antioxidant peptide, compound technology, applied in the direction of food ingredients, protein food ingredients, food ingredient functions, etc., can solve problems such as insufficient functionality of antioxidant peptides, and achieve solutions to insufficient functionality, strong instant solubility, and outstanding functions. Effect
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Embodiment 1
[0025] Example 1 Prepare the complex antioxidant peptide of the present invention according to the following steps:
[0026] 1. Preparation of zein: Take 500g of corn gluten powder, the main by-product of corn starch produced by wet method, which requires a protein content of more than 70%, and undergo a supercritical decolorization process with carbon dioxide. The reaction temperature is 45°C, the outlet valve temperature is 100°C, and the reaction pressure is 400bar, extraction time 120min, fluid flow rate 6L / h for decolorization and impurity removal. According to microwave-ultrasonic combined extraction, add 10000mL of 70% ethanol to the decolorized corn gluten powder, extract three times with a microwave reactor with 440W output power, 60s each time, and centrifuge at 4000r / min for 30min to take the supernatant for later use; Add 5000mL of 70% ethanol to the protein powder after the supernatant, re-extract with ultrasonic wave, the extraction temperature is 45°C, ultrasoni...
Embodiment 2
[0030] Embodiment 2 prepares the composite antioxidant peptide of the present invention according to the following steps:
[0031] 1. Preparation of zein: take 500g of corn gluten powder, the main by-product of corn starch produced by wet method, which requires a protein content of more than 70%, and undergo a supercritical decolorization process with carbon dioxide. The reaction temperature is 50°C, the outlet valve temperature is 100°C, and the reaction pressure is 300bar, extraction time 120min, fluid flow rate 6L / h for decolorization and impurity removal. According to microwave-ultrasonic combined extraction, add 10000mL of 70% ethanol to the decolorized corn gluten powder, extract three times with a microwave reactor with 440W output power, 60s each time, and centrifuge at 4000r / min for 30min to take the supernatant for later use; Add 5000mL of 70% ethanol to the protein powder after the supernatant, re-extract with ultrasonic wave, the extraction temperature is 50°C, ult...
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