119 results about "Virgibacillus species" patented technology

The present invention discloses one kind of Bacillius subtilis BAB-1 preserved in the preservation number of CGMCC No. 2099, and the microbial preparation prepared with Bacillius subtilis BAB-1 and its preparation process. The Bacillius subtilis BAB-1 may be applied in controlling gray mold of tomato, and has the features of high efficiency, broad bacteriostasis spectrum, simple use, low cost, no environmental pollution, etc.

The invention discloses a method for preparing a bacillus subtilis and clostridium butyricum composite bacterial preparation, which is characterized by comprising the following steps: (1) preparing seed culture solution, namely preparing a seed solution containing clostridium butyricum and a seed solution containing bacillus subtilis respectively, wherein the concentration of the bacterial solution is 0.5*10<8> to 1.0*10<8>CFU / ml; (2) preparing a coculture medium; (3) adding the seed solution of the bacillus subtilis into the coculture medium prepared by the step (1) according to a ratio of 2 percent, automatically regulating the pH value to 6.5 to 7.0 by using 10-percent diluted hydrochloric acid or 10-percent sodium hydroxide, introducing gas, stirring, controlling dissolve oxygen content to be 20 to 50 percent, culturing for 18 to 20 hours at 37 DEG C, stopping introducing gas, and continuing to stir and culture for 2 to 4 hours; and (4) adding the seed solution of the clostridium butyricum prepared by the step (1) into the coculutre medium by using a peristaltic pump according a ratio of 2 percent, standing at 35 DEG C and culturing for 24 to 28 hours to obtain the bacillus subtilis and clostridium butyricum composite bacterial preparation. In the invention, a microecological preparation is mixed with feed according to a ratio of 0.5 percent, the feed is directly used to feed livestock, the diarrhea rate and death rate of the livestock are lowered effectively, yield is increased, and medicine administration cost is saved.

The invention provides a bacillus subtilis for effectively degrading grease. The bacillus subtilis 6-loil is preserved at Guangdong Province Microbiological Culture Collection Center on December 22, 2016. The preservation number is GDMCC No. 60134. The bacillus subtilis 6-loil provided by the invention can utilize the grease as a growth carbon source, can normally grow under an environment of higher grease content and has a capacity of efficiently degrading grease. The bacillus subtilis 6-loil provided by the invention can be compounded with bacillus coagulans, brewer yeast and carrier ingredients so as to be prepared into a kitchen garbage disposal fungicide. The prepared kitchen garbage disposal fungicide can adapt to the condition of high grease content of the domestic kitchen garbage, so that the condition of failure in aerobic composting caused by the reduction of microbial activity resulted from the microorganism wrapped by the grease in aerobic composting can be effectively avoided and the bacillus subtilis can meet the requirement of the domestic kitchen garbage aerobic composting. The bacillus subtilis 6-loil also can be applied to the fields of soil oil stain treatment, biosurfactant production, biological detergent preparation, and the like.

The invention relates to a production method of bacillus subtilis antimicrobial lipopeptide and application thereof in feeds, belonging to the fields of feed additives and the biotechnology. The production method of the bacillus subtilis antimicrobial lipopeptide comprises the following steps of carrying out amplification culturing on an acillus subtilis fmbj seed culture medium, inoculating by 2-5 percent at the temperature of 28-35 DEG C, deeply fermenting 5000L of fermentor liquid for 30-48h, then extracting, and carrying out spray drying after concentration or directly carrying out spray drying to produce an antibacterial peptide product. The antimicrobial lipopeptide is added into basic daily feeds for broilers by a certain titer, thereby enhancing the feed utilization ratio and having better effect on growing development and immunity strengthening of broilers. The bacillus subtilis is a safe microorganism, and an antibacterial product thereof has high safety. In addition, compared with flavomycin used in the traditional broiler feeds, the bacillus subtilis antimicrobial lipopeptide has better effect and has an important significance on replacing feed antibiotic addition and guaranteeing food safety.

The invention discloses Bacillus subtilis for effectively degrading zearalenone and application thereof. The preservation serial number of the Bacillus subtilis ASAGF141 is CGMCC No.9463. The invention further provides bactericide containing preserved strains and a preparation method of the bactericide; besides, the invention further provides application of the strains or the bactericide to degradation of the zearalenone. According to the Bacillus subtilis for effectively degrading zearalenone and application thereof, the trains can completely degrade the 20 microgram / ml zearalenone within a short time, and the degradation rate is 10%.

Microorganisms of the genus Bacillus that are capable of reducing nitrates and contain chitin and / or chitosan in their cell walls are provided. The microorganism of the invention can be used for improving soil, for treating organic waste by fermentation, for fermenting soybeans, and for reducing bitterness. The microorganism of the invention can also be used as a feed additive or a good additive. Further, the microorganism of the invention has an anti-microbial effect.

The invention discloses a bacillus subtilis H8-3 and application thereof in preparation of a decomposed inoculant. The bacillus subtilis H8-3 is deposited with the CCTCC at Oct 21, 2013 in Hubei Wuhan university and is assigned the accession number CCTCC No:M 2013483. The bacillus subtilis H8-3 is high-temperature-resistant, can survive at 30-60 DEG C, is high in protease activity and is quick in growth. The decomposed inoculants prepared from the bacillus subtilis H8-3 is high-temperature-resistant and can secrete protease and lipase for high-efficiently fermenting animal wastes. Compost prepared through the decomposed inoculant is less in environmental pollution, is economical and is easy to use. When a product after compost treatment is subjected to subsequent treatment, the product can be employed as an organic fertilizer for achieving an object of turning waste into resources.

The invention belongs to the field of hygiene and relates to a bacillus subtillus degrading bacterial colony sensing signal and use of bacillus subtillus degrading bacterial colony as an antiseptic, in particular to bacillus subtillus degrading gram-negative bacterial colony sensing signal and the formation of a bacterial biofilm of the bacillus subtillus degrading gram-negative bacterial colony. In the use of the degrading bacterial colony sensing signal, a proper concentration of the bacillus subtillus is 5*102 to 5*104cfu / ml. The bacillus subtillus is further used as antiseptic to inhibit the bacteria such as pseudomonas, agrobacterium, enterobacteriaceae and vibrionaceae. The antiseptic can be ointment for external use, liquid preparation for external use, oral tablet, oral liquid, disinfectant, clearing agents or water quality improving agent. The consistency of the bacillus subtillus serving as the antiseptic is 105 to 106 cfu / g. The use of the bacillus subtillus provided by the invention and the antiseptic inhibit the formation of biofilm of disease-causing bacteria and the growth of bacteria by using microbial quorum-sensing inhibition instead of preventing and sterilizing bacteria by compound which is adopted by most of the conventional antiseptic; therefore, the problem of drug resistance in the antimicrobial process is avoided.

The invention discloses a preparation method of a compound microbial flocculant. The compound microbial flocculant comprises the following materials: Bacillus mucilaginosus, Bacillus subtilis, Saccharomyces cerevisiae and Penicillium diversum, wherein in the compound microbial flocculant, the total bacteria count of microbes is more than one billion per gram; Bacillus mucilaginosus accounts for 30-50% of the total bacteria count; Bacillus subtilis accounts for 10-25% of the total bacteria count; Saccharomy cescerevisiae accounts for 20-40% of the total bacteria count; and Penicillium diversum accounts for 10-20% of the total bacteria count. The method comprises the following steps: amplifying culture and solidifying compound microbial flocculant. The method improves the survival rate of the compound microbial flocculant.

The invention relates to bacillus subtilis AnPL-1 capable of generating a lipopeptid surfactant under an anaerobic condition, with a preservation number of CGMCC NO. 13461. The strain AnPL-1 is a facultative anaerobe; a temperature range for efficient production of lipopeptid under the anaerobic condition is 30-40 DEG C; a pH (potential of hydrogen) value range is 6.0-7.5; a tolerance range of NaCl is 0-5%. An emulsion coefficient of an anaerobic fermentation solution of the strain for crude oil is equal to or greater than 56%; the diameter of an oil drainage circle is equal to or greater than 15mm; the lipopeptid surfactant produced by the strain AnPL-1 in situ in a physical simulation rock core increases a crude oil recovery factor by 9.9%. The strain has important popularization and application values in the field of microbial oil recovery and environmental pollution remediation.

The invention provides a bacillus subtilis strain for reducing the ammonia nitrogen concentration of wastewater in culturing farms. The preservation number of the strain is CGMCC No. 10055. The strain is preserved in China General Microbiological Culture Collection Center in November 25th, 2014. The strain can effectively remove ammonia-nitrogen wastewater, especially ammonia nitrogen in wastewater in culturing farms, so as to purify the water, and the environmental pollution caused by wastewater in culturing farms is eliminated.

The invention discloses a construction and application of an engineering bacteria secreting and expressing chitosan deacetylase, belonging to the technical field of fermentation engineering. The construction firstly constructs a recombinant Bacillus subtilis secreting and expressing chitosan deacetylase gene by a different source, and adds a signal peptide fragment yncM in the recombinant vector for a first time, wherein the signal peptide can secrete target protein chitosan deacetylase outside cells of the recombinant Bacillus subtilis, and obtain mutants in a non-translation region at 5' end, so that the expression of the target protein is significantly increased and steps of subsequent enzyme separation and purification are greatly simplified. The enzyme activity of obtained chitosan deacetylase is up to 1548.7 U / mL and the yield of chitosan deacetylase is up to 620 mg / L when fermentation medium is fermented and cultured for 50-60 hours. And meanwhile, the method has the advantages of low production cost, mild production conditions, simple purification process, safe operation and the like.

The invention discloses Bacillus subtilis capable of producing broad spectrum and efficient antibacterial peptide and an application of the Bacillus subtilis and belongs to the technical field of microorganisms, wherein the Bacillus subtilis is Bacillus subtilis BRT39 concretely with the preservation number of CGMCC NO. 5702. Antibacterial peptide in metabolic products of a BRT39 bacterial strain has stronger anti-bacterial effect, a fermentation liquor can inhibit gram positive bacteria and also has stronger inhibiting effect on multiple gram negative bacteria and fungi, and the antibacterial peptide in the fermentation liquor has strong acid-base stability, good thermal stability, strong light stability and high organic solvent tolerance. The antibacterial peptide produced by the BRT39 bacterial strain belongs to a natural antibacterial agent, has no side or toxic effect, can be used as a food preservative, a feed additive, an agricultural biological control agent and an animal disease prevention agent and has the advantages of no toxicity, no residue, no drug resistance and high safety.

The invention discloses bacillus subtilis for degrading aflatoxin B1 and application of the bacillus subtilis, and belongs to the field of biotechnologies. Primary screening is carried out by the aid of courmarin which is used as a unique carbon source and a unique energy source, AFB1 (aflatoxin B1) degradation rates of strains are used as secondary screening indexes, and accordingly the bacillus subtilis S1 (CCTCC NO:M 2016390) capable of efficiently degrading the AFB1 can be obtained by means of screening. The bacillus subtilis and the application have the advantages that the AFB1 degradation rate of strain fermentation broth can reach 61.36%; the degradation rate can reach 80.26% after the bacillus subtilis is cultured according to an inoculation amount of 10%; the degradation rate can reach 85.65% after fermented supernatant is concentrated by 8 times; as shown in research, the aflatoxin B1 can be degraded by extracellular enzymes secreted by the bacillus subtilis S1, and accordingly the strain fermentation broth or active proteins separated from the strain fermentation broth can be used for degrading the aflatoxin B1 or preparing biological additives for degrading the aflatoxin B1.

The invention relates to a Taishan bacillus subtilis for degrading AFB1 (Aflatoxius B1). The bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) since September 13, 2013, with a preservation number of CGMCC No. 8186, and the 16srDNA is shown in SEQ.NO.1. Experiments prove that after a fermentation liquor of the bacillus subtilis works with foldy feed at a temperature of 37 DEG C for 72 hours, active substances produced by the fermentation liquor can degrade AFB1 in the moldy feed; the bacillus subtilis has the advantages of high efficiency, mild acting effect, high safety, no influence on the original quality, simplicity of operations, low cost and the like, and is suitable for scale application in feed.

The invention discloses application of bacillus subtilis and a production method for strains. The production method comprises the following steps: mixing 10 to 25 grams of corn flour, 10 to 35 grams of bean pulp, 3 to 5 grams of honey, 1 to 2 grams of sodium carbonate, 1 to 2 grams of dipotassium phosphate, 1 to 2 grams of glycine, 0.5 to 1.5 grams of calcium carbonate, 0.2 to 0.3 gram of magnesium sulfate, 0.05 to 0.15 gram of manganese sulfate, 0.05 to 0.15 gram of ferric chloride, 0.3 to 0.8 gram of sodium chloride and 1,000 milliliters of water to form emulsion, inoculating 5 to 8 percent of bacillus subtilis strains by volume of the emulsion to the emulsion, then placing the emulsion into a fermentation tank, and culturing the bacillus subtilis strains for 45 to 60 hours at a temperature of between 28 and 30 DEG C to obtain the bacillus subtilis. The invention provides a biologically-prevented strain which is suitable for special geographic-climate conditions of Xinjiang and particularly can well control soil-borne diseases of economic crops mainly comprising tomatoes and cottons for processing, provides a process method suitable for industrialized mass production, and provides base conditions for developing new biological pesticide suitable for the special geographic-climate conditions of Xinjiang.

The invention relates to the technical field of applied biology, and aims to provide a bacillus subtilis strain and application of the bacillus subtilis strain in the preparation of a deodorizing microorganism. The bacillus subtilis strain is stored in China General Microbiological Culture Collection Center (CGMCC) of China Microbiological Culture Conservation Committee, and the conservation number is CGMCCNo.13714. The adopted technical scheme includes the steps of inoculating the bacillus subtilis CGMCC.13714 to an activated culture medium to obtain an activated strain, and inoculating the activated strain to an expanded fermentation medium for culture expansion to obtain a bacterial solution; inoculating the bacterial solution to faeces, and keeping the temperature at 20-35 DEG C. The novel Bacillus subtilis strain can be applied to the biological deodorization of faeces, and the deodorization effect of single use is stable and slightly influenced by the temperature; effective deodorization can be conducted by an in-situ deodorizing method, the operation is simple and easy, no supporting equipment and technology are needed, and the investment scale is small.

The invention discloses a selenium-enriched bacillus subtilis zymolyte and a preparation method thereof. The method comprises the following steps: dipping and dissolving selenium element of selenium-enriched coal gangue by using an acid liquid, performing sedimentation treatment on heavy metals by using a phosphate solution, filtering, adjusting the pH value of filtrate to be neutral, adding a carbon source and a nitrogen source needed by the growth of bacillus subtilis to obtain a selenium-enriched culture medium, sterilizing, inoculating the bacillus subtilis to culture, collecting bacillus subtilis thallus, adjusting so that the thallus is self-dissolved, and performing enzymolysis of cell walls after adding lysozyme to obtain the selenium-enriched bacillus subtilis zymolyte which can be used for fields such as medicine, food, feed and the like.

The present invention provides a strain of Bacillus subtilis CGMCC NO.7641, wherein with the strain, oligosaccharides, antigen proteins, TI and other anti-nutritional factors can be completely degraded within 24 h, and the acid-soluble protein content can be significantly increased, such that the fermented soybean meal quality is improved significantly. According to the present invention, the method of the present invention is used to pre-treat the soybean meal before the fermentation, such that the crude protein content and the acid-soluble protein content of the obtained fermented soybean meal are significantly increased, and the trypsin inhibitor is significantly reduced; and with application of the fermented soybean meal of the present invention in preparation of feed concentrate, pet feed, aquatic feed, livestock and poultry feed and other animal feed, the macromolecular protein is subjected to low molecular weight degradation and the acid-soluble protein content is significantly increased, such that animal absorption and utilization is easily achieved, the animal growth is promoted, the digestion utilization rate and the conversion rate are increased, the feed cost is reduced, and the industrial implementation is easily achieved.

The invention discloses a preparation method of a sea cucumber epiphytic Bacillus subtilis exopolysaccharide. The method has the characteristics of simple operation and low cost, and can stably acquire the exopolysaccharide of sea cucumber epiphytic Bacillus subtilis. At the same time, the sea cucumber epiphytic Bacillus subtilis exopolysaccharide prepared by the method has strong antioxidant activity, can inhibit the formation of Vicia faba root tip cell micronucleuses, and can be widely used in the anti-tumor medicines, biological control, food preservation, corrosion prevention, cosmetic additives and other fields, thus having broad market prospects and industrial value.

The invention discloses composite antioxidation peptides and a preparation method thereof. The fermentation substrates of the composite antioxidation peptides comprise the following raw materials in parts by weight: zein, soybean hydrolyzed protein and malt protein. According to the composite antioxidation peptides and the preparation method thereof, a bacillus subtilis seed bacterium suspension is used as a zymogen, the mixed dispersion system of the zein, the soybean hydrolyzed protein and the malt protein is used as a fermentation substrate, liquid state fermentation is performed, and a bacillus subtilis seed fermentation method and an enzymatic hydrolysis substrate are combined, so that a large number of functional antioxidation protein peptides can be decomposed and modified in the fermentation process. The composite antioxidation peptides and the preparation method thereof disclosed by the invention have the advantages that the technological processes are simplified, the preparation cost is reduced, and the products are diversiform. The prepared composite antioxidation peptides also have the advantages of being prominent in functions, high in rapid dissolving property, notable in processing characteristics, and the like, are suitable for the development of functional protein peptides having the effects of reducing blood pressure, resisting weariness, neutralizing the effect of alcoholic drinks, resisting oxidation and the like. According to the composite antioxidation peptides and the preparation method thereof disclosed by the invention, alcohol soluble protein is modified by using a fermentation method, so that the research stage is a climax period, and the problem that the functions of the single antioxidation peptide are deficient is solved.

The invention relates to the field of microorganisms, in particular to an acid-yielding Enterococcus faecium, a bacteriostatic microecological preparation and application thereof. A lactic acid bacterium used is high-acid-yield Enterococcus faecium screened from an animal intestine, and the high-acid-yield Enterococcus faecium is preserved under CGMCC No.9666. The invention provides a method of using bacteriocin formed by combining the Enterococcus faecium with Bacillus subtilis to produce the efficiently-bacteriostatic microecological preparation. the Enterococcus faecium quickly proliferates in the animal intestine and generates organic acid to lower pH value of the animal intestine. Bacteriostatic effect of the bacteriocin used in a low-pH-value environment is improved remarkably, and the bacteriocin plays a role in inhibiting and killing harmful bacteria in the intestine, so that planting of probiotics such as the Enterococcus faecium in the animal intestine is promoted. The efficiently-bacteriostatic microecological preparation organically combines the bacteriocin generated by the Bacillus subtilis with the Enterococcus faecium to jointly play a bacteriostatic role, improves organism immunity, promotes productivity of bred animals and has a wide application prospect.

The invention discloses bacillus subtilis cultured by biochar and a preparation method and an application thereof. The preparation method takes biochar as an adsorption carrier, such as wood chips, animal bones, plant rhizomes and other biochar, takes any kind of bacillus subtilis strains as a starting strain, and successively comprises the following steps: (1) test tube expanding culture; (2) liquid shake flask culture; (3) seed tank expanding culture; (4) fermentation culture; and (5) drying. The bacterial density of the bacillus subtilis prepared by the method can reach 5*10<13>-1*10<15> cfu / mL. The biological adsorption and the liquid fermentation are in organic combination, the bacterial density of the liquid fermentation is improved, no any additional equipment is added, continuous culture and semi continuous culture are not used, and the high-density culture of the bacillus subtilis is achieved; and the cultured bacillus subtilis can be used as a live bacterial agent of a biofertilizer or used as a live bacterial agent of the biofertilizer with other strains.

The invention relates to the technical field of function microorganism screening and particularly provides novel bacillus subtilis SY12 with the preservation number being CCTCC No: M2015483. The bacillus subtilis SY12 has the advantages that the bacillus subtilis SY12 is capable of effectively inhibiting pathogenic bacteria such as vibrio harveyi and vibrio anguillarum, improving immunity of aquaculture animals and reducing diseases; the bacillus subtilis SY12 with high proteinase-producing and amylase producing capabilities can serve as a feed additive, so that digestive ability of the aquaculture animals is improved remarkably, feed use ratio is increased remarkably, and animal growth is promoted.

The invention discloses bacillus subtilis and an application in preventing fusarium graminearum. The preservation number of the bacillus subtilis disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.7621. As a biological prevention material against wheat scab, the bacillus subtilis has a good application prospect in developing a new biological pesticide or biological control bacterial agent.

The invention aims at providing b.subtilis spores and application of the b.subtilis spores to livestock and poultry breeding. The b.subtilis spores is screened from a fresh fecal sample of a healthy piglet, has strong capacity in producing amylase, protease and cellulase, can be applied to a livestock feed as a feed additive, can effectively improve the production performance of breading an animal, and is wide in application prospect.

The invention relates to bacillus subtilis preserved in a CCTCC with a preservation number of CCTCC M2016443 and application of the bacillus subtilis. The bacillus subtilis has higher enzymatic productivity in a microaerobic environment, and the activity of lipase is higher; the bacillus subtilis realizes the effects of reducing volatilization of ammonia gas and protecting fertilizer efficiency in fermentation of organic fertilizer; in application of deodorization and bacteriostasis of a raising farm, the number of coliform groups can be reduced, harmful microbes in environment are strongly inhibited, ecological balance of environment is adjusted, and the health of a raising environment is benefited; in application of harmless treatment of livestock and poultry corpses, oil can be effectively decomposed and exhaust of stink is reduced; in application of a biological fermentation bed, pig manure can be decomposed more thoroughly, and harmful gases such as diffused ammonia gas is reduced.

The invention relates to bacillus substilis BS01 and an inoculant thereof, as well as application of the bacillus substilis BS01 to inhibition of picked fruit pathogenic bacteria. The bacillus substilis BS01 is preserved in Microbial Culture Preservation Center, Guangdong Province (GDMCC) on August, 29, 2016, and the preservation number is GDMCC NO.60069. The in-vitro antibacterial activity of the supernatant of the fermenting liquid of the bacillus substilis BS01 strain provided by the invention on the picked fruit pathogenic bacteria, particularly colletotrichum, can reach 90 percent or above, and is about three times of the antibacterial activity of the supernatant of the fermenting liquid generated by fermentation of the common LB culture medium before optimization; and the inhibition effect of the fermenting liquid of the BS01 strain obtained by the fermentation condition after optimization on anthracnose, such as banana anthracnose, tomato anthracnose and avocado anthracnose, can reach 65 percent or above.

The invention belongs to the technical field of livestock-raising, in particular relates to the filtration of the separated bacterial strain of bacillus subtilis and an application of the bacillus subtilis in the pig manure composting production and biofermentation bed. The invention also belongs to the technical fields of agricultural microbiological application and the composting production. A bacillus subtilis dcy-1 suitable for the pig manure composting production and the biofermentation bed is obtained through separation, filtration and authentication, and the bacterial strain is persevered in China Center for Type Culture Collection (CCTCC) with the preservation serial number: CCTCC NO: M 208122. The bacterial strain is made into microbial inoculums and successfully applied to the pig manure composting production and scale raising cabiofermentation bed. The invention is suitable for the livestock feces treatment of scale raising piggeries.

The invention relates to bacillus subtilis and a culture method and application thereof. The bacillus subtilis ASD05 is preserved in the General Microbiology Center of the China Committee for CultureCollection of Microorganisms on May 15, 2019, the preservation address is No.3, No.1 Courtyard, Beichen West Road, Chaoyang District, Beijing City, and the preservation number is CGMCC No.17807. The invention further relates to the culture method and application of the bacillus subtilis ASD05. It is found for the first time that the bacillus subtilis ASD05 has the effect of adhering microorganismsrich in straw degradation effect to the surface of straw, and meanwhile has the capacity of high yield of xylanase and pectinase, cellulase activity is low, and laccase activity is avoided.