117 results about "Virgibacillus species" patented technology

The present invention discloses one kind of Bacillius subtilis BAB-1 preserved in the preservation number of CGMCC No. 2099, and the microbial preparation prepared with Bacillius subtilis BAB-1 and its preparation process. The Bacillius subtilis BAB-1 may be applied in controlling gray mold of tomato, and has the features of high efficiency, broad bacteriostasis spectrum, simple use, low cost, no environmental pollution, etc.

The invention discloses Bacillus subtilis for effectively degrading zearalenone and application thereof. The preservation serial number of the Bacillus subtilis ASAGF141 is CGMCC No.9463. The invention further provides bactericide containing preserved strains and a preparation method of the bactericide; besides, the invention further provides application of the strains or the bactericide to degradation of the zearalenone. According to the Bacillus subtilis for effectively degrading zearalenone and application thereof, the trains can completely degrade the 20 microgram/ml zearalenone within a short time, and the degradation rate is 10%.

Microorganisms of the genus Bacillus that are capable of reducing nitrates and contain chitin and/or chitosan in their cell walls are provided. The microorganism of the invention can be used for improving soil, for treating organic waste by fermentation, for fermenting soybeans, and for reducing bitterness. The microorganism of the invention can also be used as a feed additive or a good additive. Further, the microorganism of the invention has an anti-microbial effect.

The invention discloses a bacillus subtilis H8-3 and application thereof in preparation of a decomposed inoculant. The bacillus subtilis H8-3 is deposited with the CCTCC at Oct 21, 2013 in Hubei Wuhan university and is assigned the accession number CCTCC No:M 2013483. The bacillus subtilis H8-3 is high-temperature-resistant, can survive at 30-60 DEG C, is high in protease activity and is quick in growth. The decomposed inoculants prepared from the bacillus subtilis H8-3 is high-temperature-resistant and can secrete protease and lipase for high-efficiently fermenting animal wastes. Compost prepared through the decomposed inoculant is less in environmental pollution, is economical and is easy to use. When a product after compost treatment is subjected to subsequent treatment, the product can be employed as an organic fertilizer for achieving an object of turning waste into resources.

The invention discloses a preparation method of a compound microbial flocculant. The compound microbial flocculant comprises the following materials: Bacillus mucilaginosus, Bacillus subtilis, Saccharomyces cerevisiae and Penicillium diversum, wherein in the compound microbial flocculant, the total bacteria count of microbes is more than one billion per gram; Bacillus mucilaginosus accounts for 30-50% of the total bacteria count; Bacillus subtilis accounts for 10-25% of the total bacteria count; Saccharomy cescerevisiae accounts for 20-40% of the total bacteria count; and Penicillium diversum accounts for 10-20% of the total bacteria count. The method comprises the following steps: amplifying culture and solidifying compound microbial flocculant. The method improves the survival rate of the compound microbial flocculant.

The invention provides a bacillus subtilis strain for reducing the ammonia nitrogen concentration of wastewater in culturing farms. The preservation number of the strain is CGMCC No. 10055. The strain is preserved in China General Microbiological Culture Collection Center in November 25th, 2014. The strain can effectively remove ammonia-nitrogen wastewater, especially ammonia nitrogen in wastewater in culturing farms, so as to purify the water, and the environmental pollution caused by wastewater in culturing farms is eliminated.

The invention discloses a construction and application of an engineering bacteria secreting and expressing chitosan deacetylase, belonging to the technical field of fermentation engineering. The construction firstly constructs a recombinant Bacillus subtilis secreting and expressing chitosan deacetylase gene by a different source, and adds a signal peptide fragment yncM in the recombinant vector for a first time, wherein the signal peptide can secrete target protein chitosan deacetylase outside cells of the recombinant Bacillus subtilis, and obtain mutants in a non-translation region at 5' end, so that the expression of the target protein is significantly increased and steps of subsequent enzyme separation and purification are greatly simplified. The enzyme activity of obtained chitosan deacetylase is up to 1548.7 U/ mL and the yield of chitosan deacetylase is up to 620 mg/ L when fermentation medium is fermented and cultured for 50-60 hours. And meanwhile, the method has the advantages of low production cost, mild production conditions, simple purification process, safe operation and the like.

The invention discloses Bacillus subtilis capable of producing broad spectrum and efficient antibacterial peptide and an application of the Bacillus subtilis and belongs to the technical field of microorganisms, wherein the Bacillus subtilis is Bacillus subtilis BRT39 concretely with the preservation number of CGMCC NO. 5702. Antibacterial peptide in metabolic products of a BRT39 bacterial strain has stronger anti-bacterial effect, a fermentation liquor can inhibit gram positive bacteria and also has stronger inhibiting effect on multiple gram negative bacteria and fungi, and the antibacterial peptide in the fermentation liquor has strong acid-base stability, good thermal stability, strong light stability and high organic solvent tolerance. The antibacterial peptide produced by the BRT39 bacterial strain belongs to a natural antibacterial agent, has no side or toxic effect, can be used as a food preservative, a feed additive, an agricultural biological control agent and an animal disease prevention agent and has the advantages of no toxicity, no residue, no drug resistance and high safety.

The invention discloses bacillus subtilis for degrading aflatoxin B1 and application of the bacillus subtilis, and belongs to the field of biotechnologies. Primary screening is carried out by the aid of courmarin which is used as a unique carbon source and a unique energy source, AFB1 (aflatoxin B1) degradation rates of strains are used as secondary screening indexes, and accordingly the bacillus subtilis S1 (CCTCC NO:M 2016390) capable of efficiently degrading the AFB1 can be obtained by means of screening. The bacillus subtilis and the application have the advantages that the AFB1 degradation rate of strain fermentation broth can reach 61.36%; the degradation rate can reach 80.26% after the bacillus subtilis is cultured according to an inoculation amount of 10%; the degradation rate can reach 85.65% after fermented supernatant is concentrated by 8 times; as shown in research, the aflatoxin B1 can be degraded by extracellular enzymes secreted by the bacillus subtilis S1, and accordingly the strain fermentation broth or active proteins separated from the strain fermentation broth can be used for degrading the aflatoxin B1 or preparing biological additives for degrading the aflatoxin B1.

The invention discloses application of bacillus subtilis and a production method for strains. The production method comprises the following steps: mixing 10 to 25 grams of corn flour, 10 to 35 grams of bean pulp, 3 to 5 grams of honey, 1 to 2 grams of sodium carbonate, 1 to 2 grams of dipotassium phosphate, 1 to 2 grams of glycine, 0.5 to 1.5 grams of calcium carbonate, 0.2 to 0.3 gram of magnesium sulfate, 0.05 to 0.15 gram of manganese sulfate, 0.05 to 0.15 gram of ferric chloride, 0.3 to 0.8 gram of sodium chloride and 1,000 milliliters of water to form emulsion, inoculating 5 to 8 percent of bacillus subtilis strains by volume of the emulsion to the emulsion, then placing the emulsion into a fermentation tank, and culturing the bacillus subtilis strains for 45 to 60 hours at a temperature of between 28 and 30 DEG C to obtain the bacillus subtilis. The invention provides a biologically-prevented strain which is suitable for special geographic-climate conditions of Xinjiang and particularly can well control soil-borne diseases of economic crops mainly comprising tomatoes and cottons for processing, provides a process method suitable for industrialized mass production, and provides base conditions for developing new biological pesticide suitable for the special geographic-climate conditions of Xinjiang.

The invention discloses a selenium-enriched bacillus subtilis zymolyte and a preparation method thereof. The method comprises the following steps: dipping and dissolving selenium element of selenium-enriched coal gangue by using an acid liquid, performing sedimentation treatment on heavy metals by using a phosphate solution, filtering, adjusting the pH value of filtrate to be neutral, adding a carbon source and a nitrogen source needed by the growth of bacillus subtilis to obtain a selenium-enriched culture medium, sterilizing, inoculating the bacillus subtilis to culture, collecting bacillus subtilis thallus, adjusting so that the thallus is self-dissolved, and performing enzymolysis of cell walls after adding lysozyme to obtain the selenium-enriched bacillus subtilis zymolyte which can be used for fields such as medicine, food, feed and the like.

The present invention provides a strain of Bacillus subtilis CGMCC NO.7641, wherein with the strain, oligosaccharides, antigen proteins, TI and other anti-nutritional factors can be completely degraded within 24 h, and the acid-soluble protein content can be significantly increased, such that the fermented soybean meal quality is improved significantly. According to the present invention, the method of the present invention is used to pre-treat the soybean meal before the fermentation, such that the crude protein content and the acid-soluble protein content of the obtained fermented soybean meal are significantly increased, and the trypsin inhibitor is significantly reduced; and with application of the fermented soybean meal of the present invention in preparation of feed concentrate, pet feed, aquatic feed, livestock and poultry feed and other animal feed, the macromolecular protein is subjected to low molecular weight degradation and the acid-soluble protein content is significantly increased, such that animal absorption and utilization is easily achieved, the animal growth is promoted, the digestion utilization rate and the conversion rate are increased, the feed cost is reduced, and the industrial implementation is easily achieved.

The invention discloses a preparation method of a sea cucumber epiphytic Bacillus subtilis exopolysaccharide. The method has the characteristics of simple operation and low cost, and can stably acquire the exopolysaccharide of sea cucumber epiphytic Bacillus subtilis. At the same time, the sea cucumber epiphytic Bacillus subtilis exopolysaccharide prepared by the method has strong antioxidant activity, can inhibit the formation of Vicia faba root tip cell micronucleuses, and can be widely used in the anti-tumor medicines, biological control, food preservation, corrosion prevention, cosmetic additives and other fields, thus having broad market prospects and industrial value.

The invention relates to the field of microorganisms, in particular to an acid-yielding Enterococcus faecium, a bacteriostatic microecological preparation and application thereof. A lactic acid bacterium used is high-acid-yield Enterococcus faecium screened from an animal intestine, and the high-acid-yield Enterococcus faecium is preserved under CGMCC No.9666. The invention provides a method of using bacteriocin formed by combining the Enterococcus faecium with Bacillus subtilis to produce the efficiently-bacteriostatic microecological preparation. the Enterococcus faecium quickly proliferates in the animal intestine and generates organic acid to lower pH value of the animal intestine. Bacteriostatic effect of the bacteriocin used in a low-pH-value environment is improved remarkably, and the bacteriocin plays a role in inhibiting and killing harmful bacteria in the intestine, so that planting of probiotics such as the Enterococcus faecium in the animal intestine is promoted. The efficiently-bacteriostatic microecological preparation organically combines the bacteriocin generated by the Bacillus subtilis with the Enterococcus faecium to jointly play a bacteriostatic role, improves organism immunity, promotes productivity of bred animals and has a wide application prospect.

The invention relates to the technical field of function microorganism screening and particularly provides novel bacillus subtilis SY12 with the preservation number being CCTCC No: M2015483. The bacillus subtilis SY12 has the advantages that the bacillus subtilis SY12 is capable of effectively inhibiting pathogenic bacteria such as vibrio harveyi and vibrio anguillarum, improving immunity of aquaculture animals and reducing diseases; the bacillus subtilis SY12 with high proteinase-producing and amylase producing capabilities can serve as a feed additive, so that digestive ability of the aquaculture animals is improved remarkably, feed use ratio is increased remarkably, and animal growth is promoted.

The invention discloses bacillus subtilis and an application in preventing fusarium graminearum. The preservation number of the bacillus subtilis disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.7621. As a biological prevention material against wheat scab, the bacillus subtilis has a good application prospect in developing a new biological pesticide or biological control bacterial agent.

The invention aims at providing b.subtilis spores and application of the b.subtilis spores to livestock and poultry breeding. The b.subtilis spores is screened from a fresh fecal sample of a healthy piglet, has strong capacity in producing amylase, protease and cellulase, can be applied to a livestock feed as a feed additive, can effectively improve the production performance of breading an animal, and is wide in application prospect.

The invention relates to bacillus subtilis preserved in a CCTCC with a preservation number of CCTCC M2016443 and application of the bacillus subtilis. The bacillus subtilis has higher enzymatic productivity in a microaerobic environment, and the activity of lipase is higher; the bacillus subtilis realizes the effects of reducing volatilization of ammonia gas and protecting fertilizer efficiency in fermentation of organic fertilizer; in application of deodorization and bacteriostasis of a raising farm, the number of coliform groups can be reduced, harmful microbes in environment are strongly inhibited, ecological balance of environment is adjusted, and the health of a raising environment is benefited; in application of harmless treatment of livestock and poultry corpses, oil can be effectively decomposed and exhaust of stink is reduced; in application of a biological fermentation bed, pig manure can be decomposed more thoroughly, and harmful gases such as diffused ammonia gas is reduced.

The invention relates to bacillus substilis BS01 and an inoculant thereof, as well as application of the bacillus substilis BS01 to inhibition of picked fruit pathogenic bacteria. The bacillus substilis BS01 is preserved in Microbial Culture Preservation Center, Guangdong Province (GDMCC) on August, 29, 2016, and the preservation number is GDMCC NO.60069. The in-vitro antibacterial activity of the supernatant of the fermenting liquid of the bacillus substilis BS01 strain provided by the invention on the picked fruit pathogenic bacteria, particularly colletotrichum, can reach 90 percent or above, and is about three times of the antibacterial activity of the supernatant of the fermenting liquid generated by fermentation of the common LB culture medium before optimization; and the inhibition effect of the fermenting liquid of the BS01 strain obtained by the fermentation condition after optimization on anthracnose, such as banana anthracnose, tomato anthracnose and avocado anthracnose, can reach 65 percent or above.

The invention relates to bacillus subtilis BY7 and an application thereof in degrading residual feeds and ammonia nitrogen. The bacillus subtilis BY7 is preserved in China Center for Type Culture Collection on September 29, 2015, with preservation number of CCTCC M 2015585; and the 16s rNDA of the bacillus subtilis BY7 is as shown in SEQ ID NO.1. The bacillus subtilis BY7 or a BY7 suspension can degrade ammonia nitrogen in a contaminated water body or an aquaculture water body and residual feeds in fee. The bacillus subtilis provided by the invention is applicable to the following aspects: (1) the bacillus subtilis, with a strong ammonia nitrogen degrading capacity, can be used for improving an ammonia-nitrogen pollution problem in the water body and for reducing an ammonia-nitrogen content in an aquaculture pond; and (2) the bacillus subtilis, which is capable of degrading protein in the residual feeds of the feed, can be used for degrading the residual feeds in the aquaculture pond and organic matters in the bottom of the pond. The bacillus subtilis disclosed by the invention has a broad application prospect in aquaculture ponds at high temperature in the summer season.