Production method of bacillus subtilis antimicrobial lipopeptide and application thereof in feeds

A technology of Bacillus subtilis and antibacterial lipopeptide, which is applied in the field of animal husbandry, can solve the problems of food safety, such as low production of animal-derived antibacterial peptides, and achieve the effects of improving feed utilization, ensuring safety, good production and regulating immunity.

Inactive Publication Date: 2011-02-16
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of antibiotics in feed leads to food safety problems and low production of animal-derived antimicrobial peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Adopt the fermentation tank of 5000L, with 500L amplified optimal fermentation process condition to Bacillus subtilis (Bacillus subtilis) fmbJ (preservation date: June 6th, 2003, preservation number: CGMCC No.0943, see patent publication number: CN1477189 ) for fermentation production. The optimal fermentation process parameters are: temperature 28-35°C, stirring speed 180r / min, ventilation volume 80m 3 / h, tank pressure 0.10~0.12Mpa, pH7.0, soybean oil 0.3%.

[0016] Use Bacillus subtilis (Bacillus subtilis) strain fmbJ, seed medium to expand culture, seed medium: tryptone 10.0g, yeast extract 5.0g, NaCl 5.0g, distilled water 1000ml, pH7.2; volume ratio 2~5% inoculation , 28~35℃, 5000L fermenter for submerged fermentation for 30~48h, liquid fermentation medium: glucose 20.0g / L, tryptone 5.0g / L, sodium glutamate 2~5%, MgSO 4 0.5g / L, KCl 0.5g / L, KH 2 PO 4 1g / L, FeSO 4 0.15mg / L, MnSO 4 5.0mg / L, CuSO 4 0.16mg / L, pH7.0; fermentation process parameters: temperature 28...

Embodiment 2

[0019] Bacillus subtilis fmbJ was fermented and produced in a 5000L fermenter under the optimal fermentation conditions after 1000L scale-up. The optimal fermentation process parameters are: temperature 32°C, stirring speed 180r / min, ventilation volume 80m 3 / h, tank pressure 0.10~0.12Mpa, pH7.0, soybean oil 0.3%.

[0020] The industrial centrifuge used is a disc centrifuge with a rotating speed of 6000r / min to remove bacteria and then dry it by spray drying. Its process is: temperature 210 ℃, blower speed 70 revolutions / s, excipient: maltodextrin: porous starch=6~9:1, the recovery rate of antimicrobial peptide reaches 54% at this moment, antibacterial potency is 20000~ 70000IU / g dry powder.

Embodiment 3

[0022] 600 1-day-old healthy AA broilers were selected (no significant difference in body weight, P>0.05), and were randomly divided into 5 treatment groups by using a single-factor complete random experiment design. Each treatment group had 6 repetitions, and each repetition was 20 chicken. Blank control group (C group) fed basal ration (do not contain antibiotic and antimicrobial lipopeptide in basal ration), antibiotic control group (AC) added the flavomycin of 5mg / kg in basal ration, test I was in basal Antibacterial lipopeptide 4000U / kg was added to the diet.

[0023] The average daily gain of group I supplemented with 4000U / kg was the highest, which was 3.77% higher than that of group AC; the average daily food intake of group I also had a downward trend compared with group C and group AC, but the difference was not significant. The material weight ratio F / G of group I was 4.94% lower than that of group AC and 3.14% lower than that of group C.

[0024] Compared with gr...

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Abstract

The invention relates to a production method of bacillus subtilis antimicrobial lipopeptide and application thereof in feeds, belonging to the fields of feed additives and the biotechnology. The production method of the bacillus subtilis antimicrobial lipopeptide comprises the following steps of carrying out amplification culturing on an acillus subtilis fmbj seed culture medium, inoculating by 2-5 percent at the temperature of 28-35 DEG C, deeply fermenting 5000L of fermentor liquid for 30-48h, then extracting, and carrying out spray drying after concentration or directly carrying out spray drying to produce an antibacterial peptide product. The antimicrobial lipopeptide is added into basic daily feeds for broilers by a certain titer, thereby enhancing the feed utilization ratio and having better effect on growing development and immunity strengthening of broilers. The bacillus subtilis is a safe microorganism, and an antibacterial product thereof has high safety. In addition, compared with flavomycin used in the traditional broiler feeds, the bacillus subtilis antimicrobial lipopeptide has better effect and has an important significance on replacing feed antibiotic addition and guaranteeing food safety.

Description

technical field [0001] The invention relates to a production method of Bacillus subtilis antibacterial lipopeptide and its application in broiler feed, and belongs to the technical field of animal husbandry and the field of biotechnology in the field of agriculture. It involves the use of antimicrobial peptides to replace the addition of antibiotics in feed, reducing antibiotic residues in chicken and ensuring food safety. Background technique [0002] The concept of feed safety, namely food safety, has become a consensus in the world. Food and feed belong to the same scope of management in the United States and apply to the same law; in order to ensure food safety, the Danish government has formulated regulations prohibiting the use of antibiotics in feed production; the European Union has banned the use of antibiotics as feed accelerators in animal feeding. With the improvement of people's living standards, the demand for animal products is increasing, and the voice of gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17C12P21/00C12R1/125A23K20/195
Inventor 陆兆新王恬吕凤霞都海明别小妹王昱沣
Owner NANJING AGRICULTURAL UNIVERSITY
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