Selenium-enriched bacillus subtilis zymolyte and preparation method thereof

A technology of Bacillus subtilis and enzymatic hydrolyzate, which is applied in the field of selenium-enriched Bacillus subtilis enzymatic hydrolyzate and its preparation, can solve the problems that are not conducive to the production of fermented food, unavoidable residues, and human injury, etc., so as to achieve full utilization, Improved digestibility and high biomass

Active Publication Date: 2014-06-18
泰安肽普德蛋白有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the main sources of selenium are selenate and selenite, but these are highly toxic inorganic salts, which will definitely cause harm to the human body to a certain extent in the process of large-scale pro

Method used

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  • Selenium-enriched bacillus subtilis zymolyte and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0032] 1) Select 100g of selenium-enriched coal gangue with a uniform particle size and a diameter of 100 mesh, soak it in 400mL of sulfurous acid solution (concentration: 20%, v / v) for 36 hours, and use ultrasonic vibration to accelerate the dissolution of selenium-enriched coal gangue;

[0033] 2) Take 200mL sodium phosphate solution (10% concentration, v / v) to precipitate heavy metals in selenium-enriched coal gangue and filter;

[0034] 3) Adjust the pH of the filtrate to 7.0 with 0.1 mol / L NaOH solution, and add it into LB liquid medium to obtain a selenium-enriched medium (containing 200 μg / mL of selenium). LB medium: 1% (w / w) peptone, 0.5% (w / w) yeast extract powder, 1% (w / w) sodium chloride, prepared with distilled water, pH 7.0, sterilized at 121°C for 20 minutes;

[0035] 4) First-level liquid seed culture: Inoculate Bacillus subtilis ATCC6633 strain in selenium-enriched LB liquid medium at an inoculum amount of 5%, and culture it at 37°C and pH value of 7 for 20 hou...

Embodiment 2

[0045] 1) Select 100g of selenium-enriched coal gangue with a uniform particle size and a diameter of 80 mesh, soak it in 300mL sulfurous acid solution (15% concentration, v / v) for 24 hours, and use ultrasonic vibration to accelerate the dissolution of selenium-enriched coal gangue;

[0046] 2) Take 150mL sodium phosphate solution (10% concentration, v / v) to precipitate heavy metals in selenium-enriched coal gangue and filter;

[0047] 3) Adjust the pH of the filtrate to 6.0 with 0.1 mol / L NaOH solution, and add it into LB liquid medium to obtain a selenium-enriched medium. LB medium: 1% (w / w) peptone, 0.5% (w / w) yeast extract powder, 1% (w / w) sodium chloride, prepared with distilled water, pH 7.0, sterilized at 121°C for 20 minutes;

[0048] 4) First-level liquid seed culture: inoculate Bacillus subtilis ATCC6633 strain in selenium-enriched LB liquid medium (containing selenium 100 μg / mL) at an inoculum size of 5%, and cultivate it under the conditions of 37°C and pH 7.4 20h...

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Abstract

The invention discloses a selenium-enriched bacillus subtilis zymolyte and a preparation method thereof. The method comprises the following steps: dipping and dissolving selenium element of selenium-enriched coal gangue by using an acid liquid, performing sedimentation treatment on heavy metals by using a phosphate solution, filtering, adjusting the pH value of filtrate to be neutral, adding a carbon source and a nitrogen source needed by the growth of bacillus subtilis to obtain a selenium-enriched culture medium, sterilizing, inoculating the bacillus subtilis to culture, collecting bacillus subtilis thallus, adjusting so that the thallus is self-dissolved, and performing enzymolysis of cell walls after adding lysozyme to obtain the selenium-enriched bacillus subtilis zymolyte which can be used for fields such as medicine, food, feed and the like.

Description

technical field [0001] The technical field of microorganisms of the present invention relates to a method for producing selenium-enriched Bacillus subtilis enzymolyzate by using Bacillus subtilis and the obtained selenium-enriched Bacillus subtilis enzymolyzate. Background technique [0002] Selenium is one of the essential trace elements for the human body. It has the functions of maintaining the physiological functions of the heart, increasing immunity, resisting abnormal cells and mutagenic effects, resisting toxic heavy metals in organisms, anti-oxidation, anti-cancer, detoxification and detoxification, and protecting the liver. At the same time, selenium also has a certain preventive effect on cataract cardiovascular diseases, Keshan disease, Kashin-Beck disease, arthritis and other diseases. [0003] Selenium exists in two forms: inorganic selenium and organic selenium. Organic Bacillus subtilis selenium is safe and efficient. Studies have shown that the poisoning...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/125
Inventor 汪超李冬生吴珊徐宁胡勇高冰朱于鹏
Owner 泰安肽普德蛋白有限公司
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