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Bacillus subtilis strain, microecological preparation, and applications thereof

A technology of Bacillus subtilis and micro-ecological preparations, applied in the field of microorganisms, can solve the problems of difficult large-scale promotion, large amount of bacteria, and high cost

Active Publication Date: 2016-07-20
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] CN102267787A is to use microbial preparations, microbial adsorbents and filter-feeding fish to strengthen the treatment of biogas slurry, but it is necessary to add microbial preparations according to the amount of biogas slurry every day. If the daily treatment volume of biogas slurry is large, the cost is high
The ultimate goal of the above-mentioned patents is to meet the discharge standards, but cannot reduce the concentration of ammonia nitrogen in the aquaculture wastewater, and the amount of bacterial injection is large, the cost is high, and it is not easy to promote on a large scale

Method used

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  • Bacillus subtilis strain, microecological preparation, and applications thereof
  • Bacillus subtilis strain, microecological preparation, and applications thereof
  • Bacillus subtilis strain, microecological preparation, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Bacillus subtilis of the present invention reduces ammonia nitrogen ability determination

[0028] A. Test material

[0029] Tested strain: Bacillus subtilis strain CGMCCNo.10055

[0030] B. Test method

[0031] Inoculate Bacillus subtilis CGMCCNo.10055 with 1% inoculum in 200ml of fresh ammonia nitrogen selection medium (the ammonia nitrogen selection medium here is exactly the same as the above ammonia nitrogen selection medium), and culture at 30°C and 160rpm for 24h , and the concentration of ammonia nitrogen (NH4-N) and total nitrogen (TN) in the ammonia nitrogen medium was detected every 4 hours; at the same time, a blank control group was set. Among them ammonia nitrogen adopts Nessler spectrophotometry; total nitrogen adopts potassium persulfate ultraviolet spectrophotometry.

[0032] C. Test results

[0033] As shown in Table 1, when Bacillus subtilis CGMCCNo.10055 was cultured and grown in ammonia nitrogen selective culture medium with ammoniu...

Embodiment 2

[0036] Embodiment 2 Bacillus subtilis of the present invention reduces the effect of ammonia nitrogen to actual sewage

[0037] A. Test material

[0038] Sewage for testing: waste water from Fujian Xingxing Pig Farm;

[0039] B. Test method

[0040] Sodium acetate was added as a carbon source in the culture wastewater, and the final concentration of sodium acetate was 2 mg / mL. Bacillus subtilis was inoculated in 100 mL of culture wastewater with a 5% inoculation amount, and cultured at 30°C and 170 rpm, and the culture was detected after 24 hours. Ammonia nitrogen concentration in wastewater (NH 4 -N), and total nitrogen (TN) concentration; at the same time, the aquaculture wastewater without inoculation solution was used as the blank control group. Among them, the ammonia nitrogen adopts Nessler spectrophotometry; the total nitrogen adopts potassium persulfate ultraviolet spectrophotometry.

[0041] C. Test results

[0042]As shown in Table 2, when Bacillus was cultured ...

Embodiment 4

[0045] Embodiment 4 Preparation of Bacillus subtilis powder of the present invention

[0046] The Bacillus subtilis CGMCCNo.10055 bacterial classification is inoculated in soybean liquid culture medium by volume percentage 5%, and the shake flask fermentation culture, wherein, the main component of described soybean liquid culture medium is: soybean 20g / L, sucrose 20g / L L, NaCl10g / L, the fermentation temperature is 37°C, the fermentation time is 48h, the pH value is 7.0, and the rotation speed is 200rpm. After the cultivation is completed, separate the precipitate, mix it with zeolite powder at a weight ratio of 1:10, and dry it.

[0047] Embodiment 4 The effect of Bacillus subtilis powder of the present invention on reducing ammonia nitrogen in farm wastewater

[0048] A. Test material

[0049] Bacteria powder for test: Bacillus subtilis strain CGMCCNo.10055, the number of viable bacteria is 10 8 CFU / Kg

[0050] Sewage for testing: waste water from Fujian Xingxing Pig Farm...

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Abstract

The invention provides a bacillus subtilis strain for reducing the ammonia nitrogen concentration of wastewater in culturing farms. The preservation number of the strain is CGMCC No. 10055. The strain is preserved in China General Microbiological Culture Collection Center in November 25th, 2014. The strain can effectively remove ammonia-nitrogen wastewater, especially ammonia nitrogen in wastewater in culturing farms, so as to purify the water, and the environmental pollution caused by wastewater in culturing farms is eliminated.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus subtilis strain for reducing the ammonia nitrogen concentration of farm wastewater and an application thereof. Background technique [0002] With the development of society, the scale and intensification of pig raising industry has brought about a very important problem is the harmless treatment of farmed animal manure, which has become an important factor restricting ecological breeding. Aquaculture wastewater is a high-soluble organic wastewater rich in nitrogen, phosphorus, organic matter, high suspended solids, and high COD. At present, the treatment of aquaculture wastewater mainly includes: physicochemical, biochemical, and natural ecological treatment technologies. Among them, microbial treatment of farm wastewater has low cost, long-lasting effect, small environmental load, and easy formation of ecological balance in the niche of farm wastewater pools....

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C02F103/20C02F101/38
Inventor 陈丽仙余占桥
Owner BEIJING DABEINONG TECH GRP CO LTD
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