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Bacillus subtilis strain and microecological preparation and application

A technology of Bacillus subtilis and micro-ecological preparations, applied in the field of microorganisms, can solve the problems of large amount of bacteria, high cost, and difficulty in large-scale promotion, and achieve significant environmental benefits, strong ability to remove ammonia nitrogen, and pollution control effects

Active Publication Date: 2021-07-27
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ultimate goal of the above-mentioned patents is to meet the discharge standards, but cannot reduce the concentration of ammonia nitrogen in the aquaculture wastewater, and the amount of bacterial injection is large, the cost is high, and it is not easy to promote on a large scale

Method used

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  • Bacillus subtilis strain and microecological preparation and application
  • Bacillus subtilis strain and microecological preparation and application
  • Bacillus subtilis strain and microecological preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Determination of the Ammonia Nitrogen Reduction Ability of Bacillus subtilis of the present invention

[0028] A. Test material

[0029] Tested strain: Bacillus subtilis strain CGMCC No. 10055

[0030] B. Test method

[0031] Inoculate Bacillus subtilis CGMCC No. 10055 with 1% inoculum in 200ml of fresh ammonia nitrogen selection medium (the ammonia nitrogen selection medium here is exactly the same as the above ammonia nitrogen selection medium), and culture at 30°C and 160rpm 24h, and every 4h to detect the concentration of ammonia nitrogen (NH4-N) and total nitrogen (TN) in the ammonia nitrogen medium; at the same time, set a blank control group. Among them ammonia nitrogen adopts Nessler spectrophotometry; total nitrogen adopts potassium persulfate ultraviolet spectrophotometry.

[0032] C. Test results

[0033] As shown in Table 1, when Bacillus subtilis CGMCC No. 10055 was grown in the ammonia nitrogen selective culture medium with ammonium sulfate a...

Embodiment 2

[0036] Embodiment 2 The effect of Bacillus subtilis of the present invention on reducing ammonia nitrogen in actual sewage

[0037] A. Test material

[0038] Sewage for testing: waste water from Fujian Xingxing Pig Farm;

[0039] B. Test method

[0040] Sodium acetate was added as a carbon source in the culture wastewater, and the final concentration of sodium acetate was 2 mg / mL. Bacillus subtilis was inoculated in 100 mL of culture wastewater with a 5% inoculation amount, and cultured at 30°C and 170 rpm, and the culture was detected after 24 hours. Ammonia nitrogen concentration in wastewater (NH 4 -N), and total nitrogen (TN) concentration; at the same time, the aquaculture wastewater without inoculation solution was used as the blank control group. Among them, the ammonia nitrogen adopts Nessler spectrophotometry; the total nitrogen adopts potassium persulfate ultraviolet spectrophotometry.

[0041] C. Test results

[0042]As shown in Table 2, when Bacillus was cultu...

Embodiment 4

[0045] Embodiment 4 Preparation of Bacillus subtilis powder of the present invention

[0046] The Bacillus subtilis CGMCC No.10055 bacterial classification is inoculated in soybean liquid culture medium by volume percentage 5% and shakes flask fermentation culture, wherein, the main component of described soybean liquid culture medium is: soybean 20 g / L, sucrose 20 g / L, NaCl 10g / L, the fermentation temperature is 37°C, the fermentation time is 48h, the pH value is 7.0, and the rotation speed is 200rpm. After the cultivation is completed, the precipitate is separated, mixed with zeolite powder at a weight ratio of 1:10, and dried Instantly.

[0047] Embodiment 4 The effect of Bacillus subtilis powder of the present invention on reducing ammonia nitrogen in farm wastewater

[0048] A. Test material

[0049] Bacteria powder for test: Bacillus subtilis strain CGMCC No. 10055, the number of viable bacteria is 10 8 CFU / Kg

[0050] Sewage for testing: waste water from Fujian Xing...

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Abstract

The invention provides a Bacillus subtilis strain that reduces the ammonia nitrogen concentration of farm wastewater. The strain has a preservation number of CGMCC No. 10055 and was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on November 25, 2014. The bacterial strain of the invention can effectively remove ammonia nitrogen wastewater, especially ammonia nitrogen in farm wastewater, so as to purify water quality and solve the environmental pollution problem caused by farm wastewater.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus subtilis strain for reducing the ammonia nitrogen concentration of farm wastewater and an application thereof. Background technique [0002] With the development of society, the scale and intensification of pig raising industry has brought about a very important problem is the harmless treatment of farmed animal manure, which has become an important factor restricting ecological breeding. Aquaculture wastewater is a high-soluble organic wastewater rich in nitrogen, phosphorus, organic matter, high suspended solids, and high COD. At present, the treatment of aquaculture wastewater mainly includes: physicochemical, biochemical, and natural ecological treatment technologies. Among them, microbial treatment of farm wastewater has low cost, long-lasting effect, small environmental load, and easy formation of ecological balance in the niche of farm wastewater pools....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C02F103/20C02F101/38
Inventor 陈丽仙余占桥
Owner BEIJING DABEINONG TECH GRP CO LTD
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