46 results about "Bacillus strain" patented technology

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.

The present application relates to a strain of Inhernia Bacillus and its application in ramie degumming, the strain is preserved in Guangdong Microbial Culture Collection Center on September 15, 2025, and the preservation number is GDMCC NO: 66966. When the strain is applied to ramie degumming, the degumming reaction can be carried out at a higher temperature, the degumming efficiency of ramie is effectively improved, the fiber is not damaged, the quality of the fiber is improved, and the strain has high application value in ramie degumming.

This invention discloses a chitinase-producing Bacillus albus A01 and its application in the fermentation of shrimp and crab solid waste. The chitinase-producing Bacillus albus is named... Bacillus albus A01, with accession number CCTCC: M 20252571; the 16S rDNA gene sequence of this chitinase-producing Bacillus albus strain A01 is shown in SEQ ID NO.1. The fermentation agent of this strain can effectively degrade the chitin content in shrimp and crab solid waste, and improve the nutritional value of shrimp and crab fermented powder through solid-state fermentation. This invention solves the problem of unsatisfactory fermentation effects of existing feed fermentation strains, and has great application potential for improving the feed nutritional quality of shrimp and crab fermented powder and developing feed resources.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

This invention discloses a deep-sea Bacillus strain and its applications, belonging to the field of agricultural microbiology and biocontrol technology. This invention screened and identified a deep-sea Bacillus strain NT-3-7. Experimental verification showed that the deep-sea Bacillus strain NT-3-7 provided by this invention is tolerant of saline-alkali environments (it can grow in 15% NaCl and pH 9.0 environments) and possesses good nitrogen-fixing, phosphorus-solubilizing, and potassium-solubilizing abilities; it has excellent growth-promoting effects on plants, increasing plant height, taproot length, root dry weight, and stem and leaf weight of chili pepper plants. Using the deep-sea Bacillus strain NT-3-7 provided by this invention to prepare products that promote plant growth can help promote sustainable agricultural development while significantly reducing environmental pollution, showing promising application prospects.

This invention belongs to the field of agricultural microbiology technology and relates to a strain of Bacillus velezensis S-34 and its application in controlling mango leaf anthracnose. It was isolated from the rhizosphere soil of the anthracnose-resistant mango variety 'Jinhuang' and deposited at the China Center for Type Culture Collection (CCTCC) on May 29, 2025, with accession number CCTCC No. M 20251225. The Bacillus velezensis S-34 provided by this invention not only controls mango leaf anthracnose but also exhibits good antagonistic effects against various mango leaf pathogenic fungi. Furthermore, Bacillus velezensis S-34, its sterile fermentation broth, and volatile gases can inhibit the germination and epidermal penetration of Anthracnose spores, thereby inhibiting the infection process of Anthracnose spores on plants. This invention provides a new biocontrol agent and preparation method for the efficient control of mango leaf anthracnose and other foliar diseases in agricultural production, which is beneficial to the green and healthy development of the mango industry.

This invention belongs to the field of bio-fermentation technology and relates to a strain of Bacillus videmannii SXZY-01 and its applications, including: isolating and screening a strain of Bacillus videmannii capable of producing tanninase from the surface of tobacco. Bacillus wiedmannii The strain SXZY-01, with accession number CCTCC NO:M 2025151, was used to ferment a biological agent containing SXZY-01. After fermentation optimization, the tannin enzyme activity reached 40.98 U / mL. This invention applies a high-enzyme-activity solution to tobacco leaf fermentation and optimizes the fermentation process, achieving efficient degradation of tannins, significantly improving the sensory quality of tobacco, reducing the bitter aftertaste, and demonstrating outstanding technical effects. It provides a green and environmentally friendly solution for upgrading tobacco quality and is in line with the industry's high-quality development direction.

The application belongs to the field of agricultural microorganism technology, and particularly relates to a bacillus velezensis with double effects of disease resistance and growth promotion and application thereof. The strain number is BVZX-1. The bacillus velezensis BVZX-1 strain has been preserved in the China General Microbiological Culture Collection Center, the preservation date is November 7, 2025, the preservation address is No. 1, Xili, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 36546. The bacillus velezensis BVZX-1 has effects of disease resistance, growth promotion, seed germination promotion and seed aging delay.

The application discloses a bacillus velezensis agent with multiple tobacco fungal disease prevention and preparation method and application thereof. The application discloses a bacillus velezensis strain with multiple tobacco fungal disease prevention, wherein the preservation name of the bacillus velezensis strain with multiple tobacco fungal disease prevention is Bacillus velezensis YC2177, and the preservation number is CCTCC NO: M20211635. The application can improve production efficiency and product quality, reduce the incidence of fungal diseases, thereby achieving good effects in the process of biological control, preventing and controlling multiple fungal diseases while applying base fertilizer, effectively replacing the use of chemical pesticides, being green and environment-friendly, reducing environmental pollution and improving crop safety.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus safranin* and its application in the preparation of flocculants. The strain is *Bacillus safranin*. (Bacillu s safensis)BS‑RCEES‑ 2025‑001 The strain was deposited at the China General Microbiological Culture Collection Center on December 30, 2025, with accession number [accession number missing]. CGMCCNo.37242。 The present invention is based on Bacillus sarfus. (Bacillus safensis)BS‑RCEES‑2025‑001 The strain secretes extracellular polysaccharides that have a strong flocculation effect, and the flocculation rate of the wastewater treated with the flocculant prepared from it is 80% to 94%.

The application discloses a tobacco source bacillus velezensis chq3-4 and its amylase and application, and belongs to the field of tobacco microorganisms. The bacillus velezensis chq3-4 strain belongs to Bacillus, is classified and named as Bacillus velezensis chq3-4, is preserved in the China Center for Type Culture Collection, the preservation date is June 19, 2024, and the preservation number is CCTCC NO: M 20241305. The amylase crude enzyme solution prepared from the strain has an enzyme activity of 119.28+ / -0.76 U / mL, and the enzyme has strong stability and can still maintain certain enzyme activity under high-temperature and alkaline conditions. The amylase crude enzyme solution is applied to tobacco products, can effectively reduce the content of tobacco starch in the tobacco products, improve the content of reducing sugar, and then improve the quality of the tobacco products.

This invention belongs to the field of microbial technology, specifically relating to a type of endophytic Bacillus belye AN6 in rice and its applications. Specifically, this invention provides a Bacillus belye AN6, classified and named as follows: Bacillus velezensis This invention provides a novel rice endophytic Bacillus bereaves strain AN6. Through isolation, screening, identification, and experimental verification, it was found that this strain exhibits broad-spectrum resistance to various plant pathogens, including rice bacterial blight and rice blast. This strain has the ability to inhibit multiple plant pathogens and adsorb heavy metals, which is of great significance for the biological control of rice diseases and the development of green agriculture. (CCTCC accession number: M 20231886)

A strain of Bacillus STF4 and its applications, relating to the field of microbiology, this invention relates to Bacillus ( Bacillus sp. STF4 is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, on February 24, 2025, with accession number CCTCC NO: M2025290. It is used as a microbial agent to promote maize growth in saline-alkali soils and enhance maize's tolerance to salt and alkali stress. The Bacillus STF4 of this invention plays an important role in selecting microbial agents that enhance crop tolerance to salt and alkali stress and promote growth in saline-alkali soils. This not only provides important scientific basis for the development of microbial agents for saline-alkali soils but also opens up new avenues for solving the problem of crop yield reduction caused by soil salinization.

This invention relates to the field of biotechnology, and particularly to a salt-tolerant Bacillus strain and its applications. The invention applies a salt-tolerant Bacillus strain to the composting of high-salt organic waste, and provides an industrially feasible process and key parameter window. The salt-tolerant Bacillus strain is a microorganism that can maintain activity and promote rapid hydrolysis of starch and protein under high-salt / high-osmotic / low-water-activity conditions, along with a matching composting process, to achieve rapid, stable composting and low-odor treatment of tobacco waste, providing technical support for circular agriculture in tobacco-growing areas.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

The application belongs to the field of agricultural microbial technology, and particularly relates to a plant endophytic Bacillus safensis and application thereof in plant disease prevention and treatment. The plant endophytic Bacillus safensis has a strain number of BSA15, a classification name of Bacillus safensis Bacillus safensis , a preservation number of CGMCC No. 36637, and is preserved in the China General Microbiological Culture Collection Center on November 17, 2025. The Bacillus safensis has a broad-spectrum inhibitory activity on pathogenic fungi and oomycetes, and can produce a significant inhibitory effect on plant pathogenic microorganisms through multiple mechanisms. In addition, the Bacillus safensis BSA15 can be stably colonized in the plant body as an endophyte, further enhancing the disease prevention and control effect. Using the strain for disease prevention and treatment can significantly reduce the use amount of chemical pesticides, reduce pesticide residues and environmental pollution, and meet the requirements of green agriculture and sustainable development.

The application belongs to the technical field of gene detection, and particularly relates to specific primers, a Taqman probe and application of a bacillus velezensis HMB26553 strain. The specific primers and the probe sequence of the bacillus velezensis HMB26553 strain are respectively SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The primers can amplify a single unique target fragment from the bacillus velezensis HMB26553 strain, and realize specific detection of the bacillus velezensis HMB26553 strain. The primers have the characteristics of high specificity, high detection efficiency, low false positive rate, long preservation time and the like. By using the primers and through real-time quantitative PCR technology, the population quantity of the bacillus velezensis HMB26553 strain in the rhizosphere of cotton can be quickly quantified, and the primers have the advantages of high throughput, rapidness and accuracy.

The present application relates to the technical field of p-hydroxyphenylacetic acid preparation, and particularly relates to a method for preparing p-hydroxyphenylacetic acid. In the first aspect, a strain of Proteus hauseri OMK-101 is provided, which can convert L-tyrosine into p-hydroxyphenylacetaldehyde and a small amount of p-hydroxyphenylacetic acid, and the preservation number is CCTCC NO: M 20252832. In the second aspect, a strain of Bacillus tropicus OMK-102 is provided, which can convert p-hydroxyphenylacetaldehyde into p-hydroxyphenylacetic acid, and the preservation number is CCTCC NO: M 20252833. In the third aspect, the application of OMK-101 or OMK-102 in the preparation of p-hydroxyphenylacetic acid is provided. In the fourth aspect, a method for preparing p-hydroxyphenylacetic acid is provided. By establishing a mixed strain whole-cell catalytic system containing OMK-101 and OMK-102, the present application can efficiently convert L-tyrosine into p-hydroxyphenylacetic acid.

The present application discloses bacillus velezensis strain 1-1-6 and bacillus composticola strain 2-1-3 and a compound microbial inoculant and use thereof. The two strains have a significant inhibitory effect on the fusarium of the Morchella esculenta stem rot pathogen, and have a synergistic antagonistic effect when combined. The two strains are fermented together to form an inoculant, and the inoculant is sprayed on the surface of the Morchella esculenta cultivation compartment at a rate of 500 mL / m 2 The incidence of stem rot can be significantly reduced by spraying the inoculant on the surface of the Morchella esculenta cultivation compartment, providing a safe and effective microbial inoculant for the biological control of Morchella esculenta stem rot.

The present invention relates to a pharmaceutical composition and a health functional food composition for regulating and improving blood glucose, each comprising, as an active ingredient, a fermented culture supernatant of a Bacillus velezensis KMU01 strain, a concentrate thereof, a dried product thereof, a fermentation metabolite thereof, or a mixture thereof, and having the effects of regulating insulin secretion in serum and lowering fasting blood glucose.

This invention discloses a compound microbial agent for processing wastewater from Trichosanthes kirilowii seeds and its preparation method, relating to the field of environmental microbiology. The compound microbial agent is composed of saponin-degrading strains, polysaccharide-degrading strains, and decolorizing strains in a volume ratio of 3:2:1. The saponin-degrading strains are Bacillus strains, the polysaccharide-degrading strains are Pseudomonas strains, and the decolorizing strains are Rhodococcus strains. The preparation method includes: isolating and screening functional strains from Trichosanthes kirilowii planting soil and wastewater from the processing workshop; expanding the culture to a bacterial concentration of CFU / mL or higher; mixing them in a volume ratio of 3:2:1; adding a protective agent; and drying to prepare a powder with a viable count greater than CFU / g. This compound microbial agent is added to the biochemical treatment unit at 0.01%-0.05% of the wastewater volume. Microbial community acclimatization can be completed within 7-14 days, effectively improving COD removal rate, saponin degradation rate, and color removal rate. It is suitable for the efficient biological treatment of Trichosanthes kirilowii seed processing wastewater.

This invention discloses a Bacillus subtilis ( Bacillus subtle The EB-8 strain, deposited on January 14, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37320, is disclosed. The application of Bacillus subtilis strain EB-8 in the fermentation modification of tartary buckwheat flour is also disclosed. This invention's Bacillus subtilis strain EB-8 can significantly increase the amylose content of tartary buckwheat, helping to inhibit starch retrogradation and improve its freeze-thaw stability; simultaneously, it increases the total flavonoid and total phenolic content of tartary buckwheat, enhancing its antioxidant activity. Furthermore, this strain can selectively retain and enrich essential amino acids such as lysine and methionine, reduce bitter components, improve taste, and facilitate the formation of a cereal matrix suitable for cold processing, with a mild taste and enhanced oxidative defense. Overall, it achieves synergistic optimization of the functional activity and processing characteristics of tartary buckwheat flour, which is beneficial for the high-value utilization of tartary buckwheat flour resources.

The application discloses a feed bacillus NGT8 and application thereof in prevention and treatment of wheat stem base rot. Paenibacillus pabuli The feed bacillus (Bacillus NGT8) provided by the application is preserved in the Guangdong Microbial Culture Collection Center on May 7, 2025, and the preservation number is GDMCC No. 66267. The strain has good bacteriostatic effect on various plant pathogenic bacteria, has broad-spectrum bacteriostatic property, and can be used for preventing and treating plant diseases caused by the pathogenic bacteria. The NGT8 strain has good prevention and treatment effect when applied to the prevention and treatment of wheat stem base rot, and can reduce the incidence and disease index of wheat stem rot. The NGT8 strain provided by the application can be used for developing more plant pathogenic bacteria inhibitors or biocontrol agents, reducing the use of chemical pesticides and pesticide residues, and providing a new way for gradually replacing chemical control with biological control.

The present application discloses a spizizenii bacillus and application thereof in pear tree disease prevention and treatment. The spizizenii bacillus strain of the present application exhibits significant antagonistic activity to escherichia amylor and apple black rot pericarp pear variety, and can be used as a core bacterial resource for biological prevention and treatment of pear tree diseases, thereby providing a new bacterial resource and technical path for constructing a green prevention and control technology system of pear tree diseases.

This invention discloses a Bacillus velezensis strain B05 and its application in controlling soybean root rot. The Bacillus velezensis strain B05 has the accession number CCTCC NO: M 2026071. Strain B05 significantly inhibits the growth of Fusarium and other pathogens. This invention is the first to screen specific genes associated with the superior antibacterial phenotype of strain B05 and clarify their sequence characteristics. Compared with other Bacillus velezensis strains, these specific genes increase the inhibition rate of strain B05 against Fusarium to over 90%, providing targeted genes for functional optimization of Bacillus velezensis and reducing the blindness of genetic engineering.

The invention relates to bioactive polypeptides from strains of Brevibacillius laterosporus which have useful activity including pesticidal activity such as insecticidal activity, compositions comprising said polypeptides, and methods for using the polypeptides and compositions, for example in methods of controlling agriculturally-important pests including insect pests.

This invention relates to a salt-tolerant bacillus strain HSHA1-2, its inoculum, and its applications, belonging to the technical field of microbiology. The salt-tolerant bacillus (… Halalkalibacterium halodurans HSHA1-2 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 38092, on April 9, 2026. The address of the depository is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. This invention also provides a bacterial agent containing salt- and alkali-tolerant *Bacillus halophilus* HSHA1-2 and its application in degrading benzene-containing wastewater. The salt- and alkali-tolerant *Bacillus halophilus* HSHA1-2 provided by this invention exhibits excellent salt and alkali tolerance, and can stably and efficiently degrade benzene compounds under extreme saline-alkali environments.

This invention belongs to the fields of genetic engineering and bioengineering technology, specifically disclosing a method for synthesizing 2-keto-L-gulonic acid (2-KLG) using Bacillus as a substrate strain. This invention achieves single-strain, one-step fermentation for 2-KLG production using Bacillus as a substrate strain by introducing and expressing sorbitol dehydrogenase and sorbitol dehydrogenase with NAD(P)H or FADH as coenzymes in Bacillus. This invention provides a novel technical route for the microbial fermentation production of 2-KLG, with the potential to replace the traditional two-step fermentation process. It also provides reference and inspiration for the exploration and optimization of biosynthetic pathways for other important compounds.