357 results about "Bacillus strain" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention discloses bacillus capable of tolerating various organic solvents and application of the bacillus. The invention provides a bacillus sp. YL23 strain, and the preservation number of the bacillus sp. YL23 strain is CGMCC (China General Microbiological Culture Collection Center) NO.29579. Also provided is the use thereof in the preparation of recombinant microorganisms for industrial fermentation. YL23 has potential application value in the technical field of industrial biology, including that substitute raw materials such as xylose can be utilized to ferment and produce target chemicals under acidic and high-temperature conditions. The characteristic can realize high-temperature production in the field of fermentation, and the processes of sterilization and the like are reduced. Meanwhile, the acid fermentation condition and the good organic solvent tolerance characteristic are beneficial to accumulation of target products such as organic acid.

The invention discloses a bacillus strain H7 and an application thereof. The invention provides bacillus velezensis H7 or a progeny of the bacillus velezensis H7, and the preservation number of the bacillus velezensis H7 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 2025630. The bacillus velezensis H7 disclosed by the invention has a very good antibacterial effect on various common clinical pathogenic bacteria (including klebsiella pneumoniae, escherichia coli, candida albicans, enterobacter cloacae complex, acinetobacter baumannii and the like), and particularly shows a very high inhibition rate on some drug-resistant clinical pathogenic bacteria; therefore, the compound has important application value in development of drug and non-drug antibacterial products (such as medical antibacterial materials and the like), a new choice can be provided for drug and non-drug prevention and control of clinical pathogenic bacterium infection, and particularly, the compound has a supplementary effect in the aspects of reducing drug-resistant bacterium propagation risk and reducing drug dependence.

The invention relates to bacillus for producing lipopeptide, bacteriostatic lipopeptide of the bacillus and application of the bacillus to aquatic products, and belongs to the technical field of microorganisms. The bacillus velezensis is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063, and the bacillus velezensis strain FJAT-57093 is a bacillus velezensis strain FJAT-57093, is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, and has a preservation number of CGMCC No. 34063. The bacteriostatic lipopeptide is prepared from three types of lipopeptides, namely, iturin, fengycin and surfactin. The invention further discloses a preparation method of the bacteriostatic lipopeptide. The bacillus and the bacteriostatic lipopeptide have a broad-spectrum bacteriostatic effect on aquatic pathogenic bacteria, can be used for aquaculture, and have a wide application prospect.

The invention relates to the technical field of biological control of plant diseases, in particular to application of a bacillus atrophaeus strain and a preparation thereof to control wheat gibberellic disease caused by gibberellic maize. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.29799, and the strain is classified and named as the bacillus atrophaeus strain HHQGTS13-1 (Bacillus atrophaeus HHQGTS13-1). The bacillus atrophaeus strain HHQGTS13-1 is wide in application range to natural environment conditions such as temperature and pH, and has the capability of antagonizing fusarium maydis when the temperature is 10-45 DEG C and the pH value is 4-10. The bacillus atrophaeus strain HHQGTS13-1 can be used for obviously inhibiting the growth of fusarium maydis hyphae and enabling the hyphae to be deformed; the antagonistic bacillus HHQGTS13-1 strain has an obvious inhibition effect on spore germination of fusarium maydis, the spore germination inhibition rate is 97.10%, the prevention and treatment effect of the antagonistic bacillus HHQGTS13-1 strain fermentation liquor after wheat is inoculated with fusarium maydis pathogenic bacteria is 63.43%, and the antagonistic bacillus HHQGTS13-1 strain has good potential of preventing and treating plant diseases caused by fusarium maydis.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention belongs to the technical field of agricultural microorganisms, and discloses a bacillus flexus strain BJ699 and application thereof in strawberry production. Specifically, the invention provides a novel bacillus flexus strain BJ699, and the preservation number of the strain BJ699 in the China Center for Type Culture Collection is CCTCC (China Center for Type Culture Collection) NO: M20252013. The invention also provides a microbial agent containing the strain BJ699 as an active component and application of the strain BJ699 in preparation of a microbial agent for prevention and control of strawberry root rot pathogenic bacteria and / or degradation of pyraclostrobin. The new strain or the microbial agent containing the strain comes from the natural environment, has no cross resistance to existing bactericides and insecticides, has the advantages of high efficiency, low toxicity, no environmental pollution, low possibility of drug resistance generation and the like, and has a wide application prospect.

The invention provides a microbial agent as well as a preparation method and application thereof. The microbial agent comprises an artificial strain, namely a bacillus velezensis P43-DegQ strain, which is obtained by carrying out genetic engineering oriented modification on bacillus velezensis XY40-1 serving as an engineering bacterium. The method comprises the following steps: replacing an original promoter of a DegQ gene in bacillus velezensis XY40-1 with a P43 promoter, connecting the DegQ gene in the XY40-1 with a PBE-P43 vector to construct a P43-DegQ recombinant expression vector, and introducing the P43-DegQ recombinant expression vector into the bacillus velezensis to obtain a bacillus velezensis P43-DegQ strain. According to the invention, the yield of the bacillus velezensis abinogen is improved through genetic engineering oriented modification, so that the microbial control effect of a microbial agent containing the strain is improved. A resonance biopesticide suspending agent and a resonance microbial seed coating agent prepared from the microbial agent are subjected to resonance treatment, the effects of plant epidemic disease prevention and control and seed germination are greatly improved compared with the prior art, and the microbial agent has a very strong application prospect.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of bacillus velezensis fermentation liquor in prevention and control of citrus red mites. A bacillus velezensis XZT106 strain is subjected to fermentation treatment to obtain fermentation liquor without thalli, the fermentation liquor contains effective components including epothilone A and silthiopham, experiments prove that the death rate of red spiders treated by a stock solution of the fermentation liquor is 100%, the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7%, and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 500 times and the death rates of the red spiders treated by the stock solution of the fermentation liquor diluted by 200 times and 500 times are 87.8% and 66.7% respectively. In addition, it is further verified that the fermentation liquor of the bacillus velezensis XZT106 thallus does not generate the problems of drug resistance and environmental pollution in the process of preventing and treating the red spiders.

The invention provides a bacillus subtilis strain and application thereof, the bacillus subtilis strain is named as bacillus subtilis Ya3.1, is preserved in China General Microbiological Culture Collection Center on July 7, 2025, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35019; the bacillus subtilis Ya3.1 is separated from intestinal tracts of healthy hybrid sturgeons, and can be used as a probiotic additive for aquaculture, especially for sturgeon culture. The bacillus subtilis Ya3.1 provided by the invention has high safety, strong stress resistance and efficient intestinal colonization ability, and can significantly improve the activity of sturgeon intestinal digestive enzymes, improve the growth performance of sturgeons, enhance the non-specific immunity of sturgeons, improve the intestinal flora structure and significantly improve the ability to resist aeromonas bacterial infection; and a special probiotic resource can be provided for sturgeon culture.

The invention discloses a long lysinibacillus inoculant, a preparation method and application, the inoculant is prepared from a long lysinibacillus strain C1, and the preparation method comprises the following steps: culturing the strain of the long lysinibacillus strain through a tryptone soybean agar culture medium until yellow single colonies appear; inoculating the yellow single colony into serum, and carrying out seed culture by using a long lysine bacillus liquid culture medium until a logarithmic phase to obtain a bacterial solution; and inoculating the cultured bacterial liquid into a production device according to a long lysine bacillus strain, and carrying out production culture by using a long lysine bacillus production culture medium to a logarithmic phase to prepare the long lysine bacillus biocontrol inoculant. And carrying out centrifugal treatment on the obtained biocontrol microbial inoculum of the long lysine bacillus, taking a supernatant, and filtering the supernatant to obtain the long lysine bacillus biocontrol fermentation broth. The invention further discloses application of the lysinibacillus elongate microbial inoculum in antagonism of rice blast bacteria and prevention and control of rice blast.

The application discloses a bacillus velezensis and application of the bacillus velezensis in rapid increase of sauce aroma in salt-reduced soy sauce fermentation, and belongs to the technical field of food fermentation. The bacillus velezensis Bv-M1 is isolated from soy sauce fermentation mash for the first time, the bacillus velezensis strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.32853. Compared with the fermentation soy sauce without the addition of the bacillus velezensis Bv-M1 aroma-enhancing koji immersion liquid, the product has obvious increase of TTMp content and sauce aroma, and currently, there is no reported application example of the bacillus velezensis strain in application of soy sauce fermentation and sauce aroma increase.

The invention discloses a bacillus tequilensis strain HN-1 and application thereof, and belongs to the technical field of biological control of plant parasitic nematodes. The strain HN-1 is identified as bacillus tequilensis, the preservation date is July 28, 2025, the strain HN-1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20251718, and the preservation number is CCTCC NO: M 20251718. The invention discovers that the bacillus tequilensis strain HN-1 has good insecticidal activity on meloidogyne incognita and pine wood nematode for the first time; the fermentation liquor and fermentation metabolites of the strain HN-1 can be used as a liquid fungicide, or the fermentation liquor is used for preparing a solid fungicide, and the fungicide can be used for preventing and treating meloidogyne incognita and pine wood nematodes in fields.

The invention discloses a bacillus strain and application thereof, the bacillus strain comprises bacillus licheniformis X-Z9 and bacillus subtilis X-Z19, the bacillus licheniformis X-Z9 and the bacillus subtilis X-Z19 are both preserved in the China Center for Type Culture Collection, the preservation number of the bacillus licheniformis X-Z9 is CCTCC (China Center for Type Culture Collection): M 2025850, and the preservation number of the bacillus subtilis X-Z19 is CCTCC NO: M 2025851. The invention also develops a compound microbial agent based on the novel strain, and the microbial agent successfully solves the key problems of serious ammonia release, large nitrogen loss, long decomposition period and the like in kitchen waste aerobic composting (especially in combination with a film covering technology). The microbial inoculum is convenient to apply, and provides a powerful biotechnology support for efficient, environment-friendly and resourceful treatment of the kitchen garbage.

The invention provides a salt-tolerant bacillus strain Bah-38, the classification name of the salt-tolerant bacillus strain Bah-38 is the salt-tolerant bacillus strain Bah-38, the salt-tolerant bacillus strain Bah-38 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the salt-tolerant bacillus strain Bah-38 is CGMCC NO.33642. The invention further provides a preparation method of the salt-tolerant bacillus strain Bah-38. According to the invention, a confrontation culture test proves that the Bah-38 strain has a bacteriostatic effect on fusarium solani, and the inhibitory effect of the strain on the fusarium solani reaches 51.4%. In a potting inoculation test, the prevention and control effect on the root rot caused by fusarium solani reaches 70.6% after the fermentation liquor of the strain is subjected to root irrigation treatment. Meanwhile, the obvious growth promoting effect is achieved. The fusarium solani has a high and stable control effect on fusarium solani, and is suitable for agricultural production requirements.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

Engineered, modified, or mutated Bacillus strains that produce keratinases or serine proteases (KerS) that biologically degrade keratin-containing materials, such as feathers; compositions containing these Bacillus strains or the keratinases they produce, and methods for biologically degrading keratin-containing materials using these strains or keratinases.

The invention belongs to the technical field of agricultural microorganisms, and relates to a bacillus velezensis strain S-34 and application thereof in prevention and treatment of mango leaf anthracnose, the bacillus velezensis strain S-34 is separated from rhizosphere soil of a mango variety 'Jinhuang' with high anthracnose resistance, is preserved in China Center for Type Culture Collection on May 29, 2025, and has a preservation number of CCTCC No. M 20251225. The bacillus velezensis S-34 provided by the invention not only can be used for preventing and treating mango leaf anthracnose, but also has a good antagonistic effect on various mango leaf pathogenic fungi. In addition, the bacillus velezensis S-34 as well as sterile fermentation liquor and volatile gas of the bacillus velezensis S-34 can inhibit spore germination and epidermis penetration processes of the colletotrichum siamensis, so that the infection process of the colletotrichum siamensis on plants is inhibited. The invention provides a novel antibiological inoculant and a preparation method for efficient prevention and treatment of mango leaf anthracnose and other leaf diseases in agricultural production, and is beneficial to green and healthy development of the mango industry.

The invention relates to a bacillus velezensis strain B12, a fungicide containing the same and application of the bacillus velezensis strain B12. The bacillus velezensis strain B12 disclosed by the invention has an efficient and lasting antagonistic effect on septospora (especially tomato septospora), and can play a key role in biological prevention and control of tomato spot blight. Furthermore, research on endogenous metabolites shows that the volatile metabolite 4-hydroxycoumarin can remarkably improve the effects of antagonizing the tomato Acetrospora chrysosporium and preventing and treating tomato spot blight, so that the 4-hydroxycoumarin can be used for developing a more efficient compound bacterial agent. The bacterial strain and the compound bacterial agent have the characteristics of high efficiency, durability, safety and environmental protection when being used for antagonizing septospora caninum and preventing and treating tomato spot blight caused by septospora caninum, so that the bacterial strain and the compound bacterial agent have relatively high industrial value and application prospect.

The invention discloses bacillus velezensis, a microbial preparation as well as a preparation method and application of the microbial preparation, and relates to the technical field of microorganisms. The bacillus velezensis strain is preserved in the China Center for Type Culture Collection, and is named as bacillus velezensis XNW-BLSYB1, the preservation number is CCTCC NO: M 2025721, the preservation time is April 8, 2025, and the 16S rDNA sequence of the bacillus velezensis strain is as shown in SEQID No.1. The bacillus velezensis strain has the advantages that the bacillus velezensis strain is named as bacillus velezensis XNW-BLSYB1; the spore formed by the bacillus velezensis XNW-BLSYB1 strain can survive at high temperature, and can inhibit the reproductive and respiratory syndrome virus and inhibit the activity of bacteria such as streptococcus suis, infectious actinobacillus pleuropneumoniae, clostridium perfringens, pasteurella multocida and escherichia coli.

The invention discloses bacillus velezensis S1. The preservation number of the bacillus velezensis strain S1 is CGMCC (China General Microbiological Culture Collection Center) No.28915, and the preservation number of the bacillus velezensis strain S1 is CGMCC No.28915. As a microbial oil-displacing agent, the strain is applied to microbial oil recovery of oil reservoirs with high salinity and alkalinity, oxygen deficit and even oxygen-free. The invention further discloses a preparation method of the bacillus velezensis, a preparation method of bacillus velezensis fermentation liquor and application of the bacillus velezensis S1 and the fermentation liquor in oil reservoirs which are high in salinity and alkalinity, oxygen-deficient and even oxygen-free. The S1 strain disclosed by the invention can reach a stable growth period after being cultured for about 12 hours, the relatively appropriate growth temperature is 37 DEG C, and the strain shows good growth in an environment with the pH value of 6-8, can tolerate the mineralization degree of about 100000 mg / L, and can grow under an anoxic condition, so that the S1 strain can be relatively well suitable for an oil reservoir stratum environment with high salt and alkali, oxygen deficiency and even oxygen-free conditions.

The present invention relates to means and methods comprising a bacterial heme peroxidase and / or a functional fragment thereof for medical and non-medical use in preventing and / or controlling a pathogen on food, plants, or in or on the human or animal body. The present invention thus relates to novel medical and non-medical uses of a bacterial heme peroxidase and / or a functional fragment thereof for preventing and / or controlling a pathogen on food, plants, or on the human or animal body. Furthermore, the present invention relates to corresponding methods comprising a bacterial heme peroxidase and / or a functional fragment thereof for preventing and / or controlling a pathogen on food, plants or on or in the human or animal body comprising applying a sufficient amount of the bacterial heme peroxidase and / or a functional fragment thereof on said food, plants, or on mucosae, skin, in particular epidermal skin like scalp or facial skin and / or teeth to reduce the number of cells of said pathogen. In addition, the invention relates to specific heme peroxidases that have been identified to have desired properties. The invention also relates to fragments of or small heme peroxidases that have particularly advantageous properties. Moreover, the present invention further relates to corresponding compositions comprising a bacterial heme peroxidase and / or a functional fragment thereof for preventing and / or controlling a pathogen on food, plants, or in or on the human or animal body. Kits comprising components used in the methods and compositions of the invention are also provided herein. Furthermore, provided is the use of a bacterial heme peroxidase and / or of a functional fragment thereof for the preparation of the compositions of the present invention. The present invention finally also relates to a method for producing a bacterial heme peroxidase protein and / or a functional fragment thereof in an engineered Bacillus subtilis strain. The inventive use of the bacterial heme peroxidase of the present invention and / or a functional fragment thereof for preventing and / or controlling a pathogen is, without being limiting, particularly useful in ecologically compatible commercial plant / crop protection campaigns as well as in daily personal care products such as toothpaste for example.

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

Modified bacterial strain of the Pseudomonas sp. genus, the strain of Pseudomonas stutzeri deposited under Deposit Accession No. RGM3505 and a modified bacterial strain of the Bacillus sp. genus, a strain of Bacillus velezensis deposited under Accession No. RGM3504. The strains may be combined at a Pseudomonas stutzeri to Bacillus velezensis ratio from 1 :10 to 10:1 CFU / mL. The strains were modified to have greater chemotactic attraction towards plant roots and to improve the beneficial features of crops, for example, of legumes.

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.

The present application relates to a strain of Inhernia Bacillus and its application in ramie degumming, the strain is preserved in Guangdong Microbial Culture Collection Center on September 15, 2025, and the preservation number is GDMCC NO: 66966. When the strain is applied to ramie degumming, the degumming reaction can be carried out at a higher temperature, the degumming efficiency of ramie is effectively improved, the fiber is not damaged, the quality of the fiber is improved, and the strain has high application value in ramie degumming.

This invention discloses a halophilic fast-growing bacillus strain that degrades chlorinated persistent organic pollutants and its applications. The strain is *Celeribacter baekdonensis* TBC005, with accession number GDMCC No:65236. The *Celeribacter baekdonensis* TBC005 of this invention exhibits excellent degradation ability against chlorinated persistent organic pollutants. After 14 days of anaerobic culture, it shows significant degradation of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP reached 21.5%, 16.7%, and 16.9%, respectively; after 35 days of cultivation, the degradation rates of C... 10 H 16 The degradation rates of Cl6, TECP, and TCPP can reach 50.7%, 59.2%, and 61.3%, respectively, and they can be applied to the environmental pollution control and bioremediation of chlorinated persistent organic pollutants.

In accordance with the present invention, novel Bacillus strains are provided, more specifically, Bacillus strains that have extracellular hydrolase activity and that exhibit reduced malodor after incubation at least 25°C for at least 5 days in lactose-free whole milk samples compared to uninoculated lactose-free whole milk samples, compositions comprising these strains, and methods of using the novel Bacillus strains are provided.