48 results about "Kandelia candel" patented technology

The invention discloses a method for planting a mixed mangrove forest in groups. According to the method, local mangrove species are planted with row as unit; the direction of the row is parallel to the coastline; one local mangrove species is planted in each row; kandelia candel hypocotyls are inserted between the rows; the local mangrove species comprise Bruguiera gymnorrhiza, kandelia candel, Rhizophora stylosa, avicennia marina and Tung blossoms; and the mangrove species are 2-3 years old and the mangrove species in one row closest to the seat are 3 years old. The method is beneficial for bringing colony heterosis and boundary advantages of each mangrove species into full play, and has the advantages that topographies and spaces are sufficiently utilized and forest tree growth is promoted. The method has the advantages as follows: as more mature mangroves are planted in near-sea sections, the method is beneficial for protecting the kandelia candel hypocotyls and plants in the back rows and reducing impacts of tides on the mixed forest, the surrival rate of afforestation can be increased, the use of building materials is reduced, the secondary pollution is avoided and the cost is reduced.

The invention relates to a method of controlling the invasion of spartina alterniflora and restoring the ecological environment of mangrove by adopting the sonneratia apetala. The method is characterized by the steps: (1) pretreating the tidal flat of the spartina alterniflora invasion according to the requirement; (2) preparing the nursery stock of the sonneratia apetala; (3) planting the nursery stock of the sonneratia apetala according to the site condition and the thickness of the spartina alterniflora; (4) supporting and fixing the planted nursery stock; (5) pruning the undergrowth spartina alterniflora and planting the native mangrove after the crown closure and the fade-away or the complete fade-away of the spartina alterniflora. The spartina alterniflora starts the crown closure after a plantation of 1-1.5 years, the native mangrove including bruguiera gymnorrhiza and kandelia candel in the undergrowth of the spartina alterniflora can be planted after the sonneratia apetala gradually recesses to promote the formation of the community of rich biodiversity. The method adopts the fast-growing pioneer species of the sonneratia apetala to compete with the spartina alterniflora for fertilizer, water and sunlight, so as to cause the spartina alterniflora decline or death. The adopted sonneratia apetala has long growth period, large biomass, high stress resistance, easy plantation and good weed-suppressive effect.

The invention provides a method for managing spartina alterniflora. The method comprises the following steps of cutting off the spartina alterniflora; turning over intertidal zones; and planting embryonal axes of kandelia candel. Management on soil, with the depth of 0-20cm, for the spartina alterniflora is replaced by cutting off the spartina alterniflora, turning over the intertidal zones and planting the kandelia candel; the organic matter content and the N (nitrogen) content of the soil are obviously increased; the salinity of the soil is obviously improved by the high-density kandelia candel; pH (potential of hydrogen) of the soil is reduced, and is continuously reduced along with increasing of the density of the kandelia candel; influence on the P (phosphorus) content of the soil is quite low; the organic matter content of the soil with the depth of 20-40cm is increased, and is positively correlated with the density of the kandelia candel; the N content, the P content and the pH of the soil are reduced; and the pH is continuously reduced along with increasing of the density of the kandelia candel. After the kandelia candel is planted for one year, the density and the biomass of large benthonic animals are increased; and the density and the biomass of polychaetes animals, crustacean animals and other animals and the biomass of mollusk are improved. The method can be effectively used for managing the spartina alterniflora.

The invention relates to a cold-resistant acclimation method for kandelia candel seedling pot plants planted at a high latitude. The method successively comprises the following steps that firstly, kandelia candel hypocotyls developing well are collected, soaked by using seawater and transplanted to a planting field as soon as possible; secondly, flower pots are placed on the planting field and then filled with planting soil; then, the kandelia candel hypocotyls are inserted and planted in the flower pots; finally, before winter, a greenhouse is built on the planting field, it is guaranteed that tidewater can normally rise and fall to the interior of the greenhouse, and the kandelia candel hypocotyls which are inserted and planted in the flower pots are subjected to high-temperature and low-temperature alternative acclimation cultivation. According to the cold-resistant acclimation method, in the natural mudflat environment, a potting technology is used for seedling raising and acclimation at the same time, the survival rate is increased, and afforestation through kandelia candel is achieved in harsh mudflats; in the seedling raising process in winter, the environmental temperatureof the greenhouse is continuously adjusted, so that seedlings gradually adapt to the cold climate, the adaptive capacity to the lower temperature is improved, and the mangrove forest grows in a high-latitude area with the north latitude of 28-31 degrees.

The invention discloses an extraction method for mangrove plant kandelia candel leaf total protein suitable for two-dimensional electrophoresis. A traditional trichloroacetic acid/acetone precipitation method for protein extraction and a traditional phenol extraction method for protein extraction are combined, a cosolvent SDS solution is introduced, the extraction process is optimized, and the Phe-B method suitable for extraction of the mangrove plant kandelia candel leaf total protein is established. The extraction efficiency and quality of the protein are effectively improved, and the technical scheme can be specifically suitable for extraction of the kandelia candel leaf protein in two-dimensional electrophoresis. The method has the advantages of being easy to operate, high in protein extraction efficiency and fewer in interfering substance, and is suitable for materials with difficult protein extraction, low protein extraction efficiency and more interfering substances. The extracted kandelia candel leaf protein completely meets the requirements of the first direction and the second direction of two-dimensional electrophoresis, a high-quality two-dimensional electrophoresis gel map with high resolution, more clear protein points, uniform distribution and the clear background can be obtained, and experiment repeatability and stability are good.

The invention relates to a method for measuring and calculating an area of a mangrove plant kandelia candel leaf, which belongs to the technical field of coastal mangrove forest wetland ecosystem protection and primary productivity evaluation. The method comprises the steps of: firstly picking kandelia candel leaves with a given quantity, the same size and normal shapes as well as statistical significance, and measuring data of each leaf such as an area, a maximal length and a maximal width by utilizing a leaf area scanner; implementing the regression analysis through a nonlinear equation of Y=aX1b1X2ba, and determining the regression coefficients a, b1 and b2; obtaining an equation capable of measuring the area of the kandelia candel leaf: A=aLb1Wb2; and measuring the maximal length and the maximal width of a kandelia candel leaf on site, and respectively putting the measured maximal length and maximal width into the equation to obtain the area of the kandelia candel leaf to be measured and calculated. The invention provides a simple, easy and precise method for measuring and calculating the kandelia candel leaf, so that a more convenient technology and a method for supporting the evaluation on the primary productivity of the mangrove forest ecosystem are provided.

The invention discloses a method for preparation of an activated carbon nano-iron composite material from kandelia candel leaf. The method adopts activated carbon as the carrier, uses polyphenol, flavone, caffeine and other bioactive reductants contained in a kandelia candel leaf extracted liquid to reduce ferrous ions into zero valent nano iron. The activated carbon nano-iron composite material combines the adsorption properties of activated carbon with the strong reducibility of nano-iron organically, and improves the adsorption and reduction properties of the composite material when appliedto printing and dyeing wastewater treatment. The method provided by the invention has no need of using potassium borohydride, sodium borohydride or other reducing agent, is low in production cost andhas small harm to the environment, the prepared activated carbon nano-iron composite material overcomes the disadvantages of easy inactivation and agglomeration of nano-iron in water, difficult recovery and recycling and the like currently, and the method has high economic and environmental benefits.

The present invention discloses a method for removing sporobolus alterniflorus. The method for removing the sporobolus alterniflorus requires the following devices and apparatuses including a mower, arotary cultivator, a ditching and soil covering machine, an agricultural film covering machine, a mulching film perforating machine and a tree support fixer, and requires the following materials including black degradable shading films, mature plants and seedlings of avicennia marina, paulownia fortunei, rhizophora stylosa, kandelia candel and bruguiera gymnorrhiza, and several meretrix lyrata, glauconome chinensis, nassariidae and cassidula nucleus. The method for removing the sporobolus alterniflorus adopts a method of treating the sporobolus alterniflorus and then burying the treated sporobolus alterniflorus firstly and then putting the rhizophoraceae mature plants and seedlings and accompanying marine animals, completely removes the sporobolus alterniflorus, while does not affect themarine ecosystem and surrounding environment, at the same time is free of danger of introducing foreign invasive species, can establish a complete mangrove forest ecosystem and is conductive to ecological restoration of coastal beaches and shoals.

The invention provides a method for extracting oleanolic acid from Kandelia leaves, and relates to an oleanolic acid, in particular to a method for extracting purified oleanolic acid from mangrove Kandelia leaves. The invention provides the method for extracting oleanolic acid from Kandelia leaves, which is simple in steps, short in production cycle, high in separation efficiency and high in purity. The method comprises the following steps: Kandelia leaves are washed, dried in the shade and added with methylene chloride for extraction; extract is obtained through filtration and then dried, so as to obtain extractum; the extractum is added to silica gel for chromatography, absorbed and ground into powder; the powder is packed in a column; dient elution is performed by taking petroleum ether/acetone as eluant; 1 to 5 column volumes are rinsed according to that the mass ratio of the petroleum ether to the acetone is 30:1, 10:1 and 4:1 respectively; the powder is washed with methanol, so as to obtain 18 components in all, wherein twelfth to seventeenth components are crude oleanolic acid products; after silica gel column chromatography, and the crude oleanolic acid products are eluted by taking chloroform/methanol as the eluant, and are finally washed with methanol, so as to obtain four components, wherein a third component is just a pure oleanolic acid product.

The invention discloses ocean phosphorus-dissolving bacillus megaterium and an application thereof. The ocean phosphorus-dissolving bacillus megaterium is collected in China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Luojia Hill, Wuhan, China, and the collection number is CCTCC NO:M2018056. The ocean phosphorus-dissolving bacillus megaterium and the application disclosed by the invention have the beneficial effects that phosphates can be degraded, the phosphorus-dissolving bacillus is about 117.14mg/dl when sodium phytate is used as a unique sphosphorus source, and by high-efficiency degradation of substances such as phosphates in a deposit environment, high-efficiency circulation of phosphorus elements in an ecological system is promoted, and the obtainedphosphorus amount in the deposit environment is increased; the growth of mangrove-plant kandelia candel can be better promoted, the productivity of the whole ecological system can be improved and therestoration of the ecological system can be accelerated; due to no toxic substances, the ocean phosphorus-dissolving bacillus megaterium can be further applied to research and development of microbial agents with microbial functions, has large potential for assisting protection and restoration of the mangrove ecological system, and has better market prospect, economic benefit and ecological effect.

The invention provides a method for raising seedlings of plant kandelia candel fruit in a mangrove forest, which is used for planting the kandelia candel in a region from the north of 25 degrees northlatitude to 40 degrees north latitude. The method comprises the following steps: 1, collecting kandelia candel fruit, 2, after October, arranging a constant temperature shed on an empty land of a coastal beach; 3, soaking kandelia candel seeds in potassium permanganate solution for 30 minutes, and then taking out the seeds and airing for later use; 4, screening the kandelia candel fruit which isdamaged or rotted, and inserting the intact kandelia candel fruit into the sea silt in the constant temperature shed with the roots downwards to carry out seedling raising so that the kandelia candelfruit grows into a kandelia candel seedling; 5, transplanting the kimchi seedlings, and enabling the kandelia candel seedlings to adapt to the temperature transition from 15 DEG C of the constant-temperature shed to 10 DEG C of outdoors to 0 DEG C of the seawater so as to adapt to the local climate temperature. The method is simple, easy to carry out and low in cost, and the risks of high cost, high death rate and the like which frequently occur in manual seedling digging, transportation, planting and the like in the transplanting process are avoided; and meanwhile, the survival rate of the kandelia candel seedlings in high-latitude areas is ensured.

The invention relates to the field of pond returning for recovery of forests and wetlands and discloses a method for returning ponds for recovery of forests and wetlands. The method comprises the steps of selecting the fish ponds with the bottom elevations lower than a high tide line, forming gaps to completely or partially discharge water in the fish ponds, and then selecting mangrove plant seedlings for planting according to different elevations, wherein pioneer tree species are mainly planted in a middle-low tide zone, kandelia candel and/or rhizophora stylosa is planted in a middle tide zone, at least one of bruguiera gymnorrhiza, bruguiera sexangular and ceriops tagal is mainly planted in a high tide zone, and at least one of excoecaria agallocha, lumnitzera racemosa and semi-mangrove plants is mainly planted on the banks of the fish ponds. By adopting the provided method for returning the ponds for recovery of forests and wetlands, not only can the survival rate of afforestation be increased, but also the manufacturing cost is reduced, the heterogeneity of the ecological environment can also be obviously improved, and a foraging and habitat space is created for birds, fishes and benthic animals; the requirements of a wetland remediation principle which adopts ecology as the top priority, natural remediation as the main content and artificial auxiliary remediation as the auxiliary content are met, and the wetland remediation purpose of building forests and wetlands on appropriate sites is achieved.

The invention relates to a preparation method of high-purity general flavone in kandelia candel leaves. The preparation method disclosed by the invention comprises the following steps of: (1) crushing kandelia candel leaves into coarse powder, extracting by using 15 times of ethanol solution with the volume fraction of 60-70% for two times, wherein the extraction time for each time is 0.5-1.5h; the extraction temperature is 60-80 DEG C, and mixing extracting solution; (2) concentrating the extracting solution obtained in the step (1) till the concentration of the extracting solution is 5 mg/ml, centrifuging to obtain supernate, purifying by using nonpolar macroporous adsorption resin, adsorbing for 30 min, washing by using water to remove impurities, and eluting by using 35-80% of ethanol, wherein the nonpolar macroporous adsorption resin is one or more of HPD722 type, adsorption resin HPD100 type adsorption resin and HPD826 adsorption resin; and (3) collecting eluant obtained in the step (2), decompressing to recycle a solvent, drying, and crushing to obtain the high-purity general flavone. The content of general flavone in kandelia candel leaves is at least 60%; the method disclosed by the invention is simple, convenient, high-efficiency, environmentally friendly and high in pertinence; and thus, high-purity general flavone in kandelia candel leaves having high skin whitening and freckle removing activities can be obtained.

The invention belongs to the field of mangrove forest ecosystems, and relates to a method for simulating and predicting the total phosphorus concentration change of kandelia candel mangrove forest sediments. The method includes: constructing a kandelia candel ecological process model and a kandelia candel mangrove forest sediment phosphorus circulation model; the influence of the kandelia candel growth process on the kandelia candel mangrove sediment total phosphorus concentration is more accurately considered; on the basis of collecting and analyzing phosphorus elements in columnar sedimentsof the mangrove forest kandelia candel wetland, Model parameters are calibrated and a model is verified, and three methods of correlation analysis, root-mean-square error and observed value standard deviation ratio and consistency index are adopted to carry out precision analysis on the model, so that necessary theoretical reference and technical support are provided for research and management ofmangrove forest sediments.

The invention relates to the field of Kandelia candel processing, and in particular relates to an automatic conveying device for Kandelia candel. The automatic conveying device comprises an unloadingconveying belt, a transportation conveying belt, a guide plate, a blanking sliding way, a distance measuring sensor, a first drainage mechanism and a second drainage mechanism, wherein the transportation conveying belt is arranged obliquely, and the downstream end of the transportation conveying belt is upturned; the blanking sliding way is obliquely arranged on one side of the transportation conveying belt, and the first drainage mechanism and the distance measuring sensor are arranged between the blanking sliding way and the transportation conveying belt; the second drainage mechanism is arranged at the downstream end of the blanking sliding way; a plurality of stirring plates distributed at equal distance in the conveying direction are arranged on the conveying plane of the transportation conveying belt, and a storage space is formed between every two adjacent stirring plates; the guide plate is obliquely arranged at the downstream end of the transportation conveying belt; a first gravity sensor and a second gravity sensor are respectively arranged in the first drainage mechanism and the second drainage mechanism; and a vibration motor is arranged at the bottom of the blanking sliding way. The automatic conveying device can transfer the single Kandelia candel in a set attitude.

The invention discloses an ecological effective technology for making spartina alterniflora naturally die. The technology comprises the steps that seedlings of kandelia candel, avicennia marina and aegiceras corniculatum are selected from two ways; for the first way, protogenesis fruits of kandelia candel, avicennia marina and aegiceras corniculatum in the south of the area at the northern latitude of 20 degrees are collected, and seed cultivation is conducted at the line spacing of 20 cm*20 cm at the coastal beach in the south of the area at the northern latitude of 40 degrees; for the secondway, kandelia candel, avicennia marina and aegiceras corniculatum saplings cultivated by farmers at the coastal beach area in the south of Wenzhou in Zhejiang are planted at the beaches of all areasfrom Nantong to Lianyungang in Jiangsu at the line spacing of 1 m*1 m, survival fruit protogenesis seedlings are used for replenishment, and a blocking fence is used at the periphery for preventing various floats in seawater from flooding the saplings. According to the ecological effective technology, a large number of three kinds of plants, namely kandelia candel, avicennia marina and aegiceras corniculatum, in mangrove forest are planted, and spartina alterniflora rhizomes cannot survive but natural die by using root system extension of the three kinds of trees; spartina alterniflora is treated, the balance of the beach ecological environment is restored, and the ecological environment free of pesticide and capable of killing spartina alterniflora is truly provided for the sea.

The invention discloses a kandelia candel planting cluster forest belt for a mudflat mangrove forest in a high-stormy-wave strong-tide area. By controlling the planting density of planting clusters and spacing of the planting clusters and cooperating with a protective enclosure, the planting clusters can resist the impact of seawater and floaters within 1-2 years, the quantity of planktons and marine products in the tidal flat area is effectively increased, and habitats are provided for birds. A practical basis is provided for tidal flat reconstruction.

The invention provides a method for cultivating kandelia candel seedlings through cultivation tail water, and belongs to the technical field of plant cultivation. The cultivation tail water is used for providing nutrient substances for growth of the kandelia candel seedlings, meanwhile, the cultivation tail water is supplied regularly and quantitatively by depending on the cultivation nursery garden, it is guaranteed that the kandelia candel can adapt to the growth environment quickly in the seedling growth period, an overwintering greenhouse is built in winter of cultivation, and the situation that the kandelia candel is frozen due to plants is avoided. After one year of culture of regularly and quantitatively supplying culture tail water, an overwintering greenhouse is not built for natural overwintering in the next year of overwintering, so that the kandelia candel seedlings gradually adapt to the winter temperature of the planting land, the purpose of cold winter domestication is achieved, and it is guaranteed that the kandelia candel seedlings can adapt to the cold winter temperature of the planting land after being transplanted. Experiments prove that the method not only greatly improves the growth speed of the kandelia candel seedlings, but also improves the transplanting survival rate of the kandelia candel seedlings, and is of great significance in promoting large-scale cultivation of the kandelia candel in the Zhebei region.

The invention provides endophytic fungi 2cpe-1 of kandelia candel of mangrove plants, fermenting liquid and application thereof. With the collection number of CGMCC No. 15388, the endophytic fungi 2cpe-1 of the kandelia candel of the mangrove plants is collected at China General Microbiological Culture Collection Center on the 7th February, 2018. The invention aims at providing the endophytic fungi 2cpe-1 of the kandelia candel of the mangrove plants and the fermenting liquid thereof. The endophytic fungi 2cpe-1 is a strain with more obvious inhibiting effects for the growth of hyphae of plantpathogenic fungi such as fusarium graminearum, phytophthora sojae and colletotrichum musae, can used as a novel agricultural antibiotic natural active substance for resisting the diseases of the plant pathogenic fungi, and belongs to the technical field of microorganisms.

The invention discloses a mangrove forest afforestation method for intensively weathered tuff geology and intertidal zones with high elevations. The method comprises the steps of a, site preparation,b, planting soil backfilling, c, water channel excavation and d, mangrove plant planting. One year after afforestation is completed, various mangrove nursery stocks grow well, the survival rate of avicennia marina, kandelia candel and Bruguiera gymnorrhiza is not lower than 60%, the survival rate of Rhizophora stylosa and aegiceras corniculatum is not lower than 70%, and the height of the nurserystocks is increased by 10-30 cm. By means of the method, the area of the mangrove forest in coastal areas in southeast and south China can be enlarged, and the important functions of the mangrove forest in the aspects of food supply, germplasm resource preservation, species diversity maintenance, wind prevention, wave dissipation, marine environment purification, ecological balance maintenance, carbon sink maintenance and the like are better played; meanwhile, the created water channel and mangrove forest coexist, diversified habitats are provided, and biodiversity conservation and recovery are better facilitated.

A plant extraction used for reconditioning of cellular polystyrene and its preparation method and assemblage are disclosed. It is produced by: grinding the leaves of Eucalyptus citriodora Hook, Eucalyptus exserta F.Muell, Eucalyptus robusta Smith, kandelia candel, rhizophora stylosa, mint herb, soaking with water, distilling, collecting the oil, preparing the combination of materials adding in the accessories and the water by dispersing at high rate. Its advantages include: innocuous, contamination free, converting the huge waste cellular polystyrene into small rapidly, reclaiming and utilizing easily, volatilizing easily so as not to pollute the reclaimed waste cellular polystyrene, less dosage, low cost, conveniently using without heating and compressing, not having the cellular polystyrene analyzing, having high purity of the reclaimed product.

The invention discloses a method for preparing an activated sludge cuprous oxide composite material by using kandelia candel hypocotyl extract. The method comprises the steps of by taking activated sludge as a carrier, drying the activated sludge of municipal sewage treatment plants, crushing, screening, soaking by using nitric acid, carrying out suction filtration and drying for later use; and reducing copper ions to nano-cuprous oxide by adopting bioactive reducing agents such as polysaccharide, amino acid, vitamin C and the like contained in the kandelia candel hypocotyl extract. The methodhas the beneficial effects that the adsorption performance of the activated sludge and the high catalytic activity of cuprous oxide are organically combined by the activated sludge cuprous oxide composite material, so that the adsorption and reduction properties of the composite material applied to printing and dyeing wastewater treatment are improved; chemical reducing agents such as sodium borohydride and the like are not needed, so that the harm to the environment is less and the production cost is low; the shortcomings that the current cuprous oxide recovery is difficult, the secondary pollution is easy to form and the like are overcome by means of the prepared activated sludge cuprous oxide composite material; and therefore, the method has high environmental benefit and economic value.

The invention discloses that a kandelia candel extract in petroleum ether has a good inhibitory effect on the proliferation of prostatic cancer cells. Accordingly, the inventor also establishes the corresponding preparation method of the extract. The experiments show that the kandelia candel extract in petroleum ether has resistance effect on prostatic cancer. It can be seen that kandelia candel has the potential value of anti-prostatic cancer drugs. Therefore, the invention provides a strong basis and reliable guarantee for further opening up the medical application of mangrove plant kandeliacandel, and also opens up a Chinese herbal medicine approach for the treatment of prostatic cancer.