The invention relates to the technical field of
plant tissue culture and endangered tree seed breeding, in particular to an efficient
embryo germination culture method for breaking through the deep
dormancy characteristic of
tilia amurensis, and aims at solving the problems that existing
tilia amurensis seed breeding is low in
germination rate, long in period, high in
pollution rate and poor in
embryo seedling quality. The method comprises the following steps: selecting robust
tilia amurensis seed trees in specific areas such as 129 degrees, 19 '+ / -0.5 degrees and the like of the east
longitude, and harvesting seeds with the
water content of 45.25%-59.06% from August 29 to September 20; stratification is conducted at the low temperature of 4 DEG C for 20 days, 50 mg / L GA3 is added, soaking is conducted for 12 h, and pretreatment Precisely disinfecting with 75%
alcohol and 2%
sodium hypochlorite;
cutting a 1-2mm notch on the seed coat, and then carrying out
antioxidant treatment by using a
vitamin C-
humic acid solution; inoculating to an optimized MS culture medium containing hormones such as 0.1 mg / L 6-BA, carrying out dark culture at 22 DEG C for 5 days, and then carrying out 2000lx illumination culture at 24 DEG C; according to the method, deep
dormancy is efficiently broken, the
pollution rate is low,
embryo seedlings are robust, and large-scale breeding and
population recovery of tilia amurensis can be supported.