256 results about "Oxaloacetic acid" patented technology

The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).

Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate α-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and / or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

The invention discloses a method for constructing an ALA production strain, that is, enhancing the activity of related enzymes promoting the synthesis of oxaloacetate in the 5-aminolevulinic acid production strain or introducing exogenous related enzymes promoting the synthesis of oxaloacetate , such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and / or weaken the succinyl-CoA downstream metabolic pathway-related enzymes in the strain, such as succinyl-CoA synthetase or succinate dehydrogenase active. The invention also discloses an ALA high-yield strain constructed by the method and a method for preparing ALA by using the strain. The bacterial strain of the invention can produce ALA efficiently, at low cost and with low pollution without adding exogenous succinic acid.

Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate α-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.

The present invention describs methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-l,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

The invention relates to the field of medicaments and discloses an antialcoholismic composition. The raw materials of the antialcoholismic composition comprise: one or a mixture of more than two of S-adenosyl methionine, cysteine and methionine, one of a mixture of D-glyceric acid and D-glycerate, one or a mixture of more than two of succinic acid, fumaric acid, citric acid, oxaloacetic acid and malic acid, one or a mixture of more than two of vitamin B1, vitamin B6 and vitamin B2, one or a mixture of coenzyme Q10 or coenzyme I, L-glutamine, fortune eupatorium herb, artemisia capillaries, white paeony root and root of tall monkshood. The invention also provides a preparation method of the antialcoholismic composition. The antialcoholismic composition disclosed by the invention is taken after wine drinking for reducing absorption of alcohol, accelerating alcohol metabolism, as well as tonifying stomach and spleen and preventing drunkenness and alcoholism. The antialcoholismic composition is quick in effectiveness and has a bright application prospect.

The invention relates to a preparation for improving the color and sweet of fruits such as apple and pear, the preparation is aqueous solution agent. The red rate and sweetness of the fruit can be improved through establishing the metabolism control technology of synthesis of apple and pear color and synthesis of soluble sugar. The components and the concentration of the preparation are as follows: acetylsalicylic acid is 1.0 to 3.0 mg / L, galactose is 0.5 to 1.5 g / L, malic acid is 0.1 to 3.0 g / L, phenyalanine is 0.1 to 1.0 g / L, oxaloacetic acid is 0.1 to 1.0 g / L, boric acid is 0.1 to 2.0 g / L,monopotassium phosphate is 0.8 to 1.5 g / L, copper sulphate is 0.1 to 2.0 g / L, ammonium molybdate is 50 to 200 mg / L, twee 100 is 1.0 to 4.0 ml / L, little ethanol, and the balance water. The preparationmethod is as follows: 1, weighing up the acetylsalicylic acid, galactose, malic acid, phenyalanine, oxaloacetic acid, boric acid, monopotassium phosphate, copper sulphate, ammonium molybdate and twee100 according to a certain proportion, 2, the acetylsalicylic acid is dissolved by the ethanol, the phenyalanine is dissolved by distilled water at 100 DEG C, the monopotassium phosphate, copper sulphate and ammonium molybdate are dissolved by distilled water at 80 to 90 DEG C, other components are dissolved by distilled water at room temperature, all the components are dissolved and mixed, and then are stirred evenly to make the volume to the required volume, thereby obtaining the preparation. The preparation can improve the coloring rate of Nanguo pear by 15.8 percent. The preparation is convenient and time-saving to use, has no chemical injury and has no toxic side effect to human body and environment.

The invention discloses a rice heavy metal absorption blocking agent and a use method thereof. The blocking agent includes one or more effective components of alpha-ketoglutarate, glycerol and oxaloacetic acid. The aim of preventing heavy metal from being absorbed and transferred to rice can be achieved through leaf surface spraying in the rice tillering period, heading period and / or postulation period. The contents of lead, cadmium and other heavy metal polluting rice in rice fields can be remarkably reduced, and rice produced in rice fields slightly or intermediately polluted by heavy metal meets the food safety standard. The effective spraying concentration of the blocking agent is low, the blocking agent can be sprayed after being mixed with pesticides for preventing and treating rice diseases and pests, and cost is low.

A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.

The present invention relates to oxaloacetate compounds that activate AMP-activated protein kinase (AMPK), including the preparation of the compounds, compositions containing the compounds, preserving said compounds and the use of the compounds in the prevention or treatment of disorders such as diabetes, metabolic syndrome, obesity, cardiovascular disease, Alzheimer's disease, and cancer.

The invention relates to a kind of enzyme colorimetric method and a method of the coupling method measuring the density of citric acid and a diagnosis reagent box applying the citrate synthetase and coupling malic dehydrogenase enzymatic reaction continues monitoring method / ratio colorimetric method. The reaction of citrate synthetase enzymolysis citric acid generates oxaloacetic acid and then through the effect of coupling malic dehydrogenase at last oxidizes the reduced coenzyme (there is a absorption peak at the site of 340nm) becoming the coenzyme (there is a absorption peak at the site of 340nm) so we can assay the degree / speed of the fall-way absorbance at the place of 340nm. It can measure and calculate the density of citric acid by measuring the degree / speed of the fall-way absorbance at the place of 340nm. The method has high specificity and it is not polluted by the endogenous and exogenous object and the test result is precise and accurate. The invention can get the array result by the ultraviolet / visible light analytic instrument so it is convenience to extend and apply.

The present invention relates to a plant nutrient active agent, it is an aqueous solution containing several components. It is characterized by that it contains at least two preservative components, the described preservative component is carboxylic acid or carboxylate containing no benzene ring, and its concentration is not less than 0.001 mole / L. The described preservative component can be two kinds or more than two kinds selected from acetic acid, acetate, diacetic acid, diacetate, dehydroacetic acid, dehydroacetate, oxaloacetic acid, oxaloacetate, propanoic acid, propionate, acrylic, acid, acrylate and others.