5 results about "Oxaloacetic acid" patented technology

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.

The application provides a CO2 capture method and a CO2 capture device coupled with electrochemical regeneration of hydrogen. The method comprises the following steps: absorbing CO2 in a CO2-containing gas by using an alkaline solution to form a CO2-rich solution; performing acid-base neutralization of the CO2-rich solution and an acidic solution to obtain a neutralization solution and CO2; performing electrolysis of the neutralization solution to obtain a cathode electrolysis product and an anode electrolysis product, wherein the cathode electrolysis product is H2 and the alkaline solution, and the anode electrolysis product is O2 and the acidic solution; and the acidic solution is selected from one or a combination of more than two of the following solutions: pyruvic acid solution, gluconic acid solution, malonic acid solution, tartaric acid solution, glyceric acid solution, oxalacetic acid solution, formic acid solution, isopropionic acid solution, isobutyric acid solution, fumaric acid solution, aconitic acid solution, isocitric acid solution and hexanediol diacid solution. The acidic solution generated by electrolysis can avoid corrosion of the device equipment and reduce the net energy consumption and net cost of the CO2 capture process.

The application discloses a high-yield beta-alanine engineering bacterium and method based on a type I CRISPRi screening system, wherein by knocking out a pckA gene encoding oxaloacetate to phosphoenolpyruvate and a nadB gene encoding L-aspartate to oxaloacetate, a countercurrent pathway for synthesizing beta-alanine is blocked, and carbon flow loss is reduced; by knocking out an ldhA gene and a poxB gene, the content of synthesized pyruvate is increased; by overexpressing an NCgl0580 gene, the tolerance of EcN to products is improved; by knocking out a lacI gene in a genome, the adding cost of an inducing agent in a fermentation process is reduced; by optimizing a type I CRISPRi system for high-throughput screening of target genes affecting the yield of beta-alanine in the engineering bacterium, the screening efficiency and accuracy are significantly improved, an aspartate tRNA ligase gene aspS capable of improving the yield of beta-alanine is obtained, by overexpressing the aspS, the PanD enzyme activity is increased, and the yield of beta-alanine is further improved.

The application relates to a phosphorus-free neutral rust remover and a preparation method, and relates to the technical field of rust removal.The phosphorus-free neutral rust remover comprises the following components in proportion by weight: 5-10 parts of sodium hydrosulfite, 5-10 parts of phosphorus-free washing agent, 0.5-2 parts of dispersant, 10-20 parts of phosphorus-free chelating agent and 67-84 parts of water; the phosphorus-free washing agent is obtained by compounding oxalacetic acid and polyether compounds.The specific phosphorus-free washing agent obtained by compounding oxalacetic acid and polyether compounds, the specific reducing agent of sodium hydrosulfite, the phosphorus-free chelating agent and the dispersant are compounded to prepare the phosphorus-free neutral rust remover.The phosphorus-free neutral rust remover can selectively and efficiently remove iron rust on the surface of stainless steel and will not damage the metal base, and the problems of low safety, poor rust removal effect and corrosion of the metal base of the existing rust remover are solved.

The present invention is directed to methods of controlling the growth of undesired vegetation in a particular area by using an herbicidal composition that contains an esterified plant metabolite.