4results about How to "Reduce purification" patented technology

The invention belongs to the field of biological catalysis, and particularly relates to a method for preparing oxidized nicotinamide adenine dinucleotide phosphate through double-enzyme coupling. According to the invention, through construction and optimization of a one-pot double-enzyme coupled catalytic system, a genetically engineered bacterium containing nicotinamide mononucleotide adenosine transferase NMNAT and a genetically engineered bacterium containing NAD kinase are used as a whole-cell catalyst, nicotinamide mononucleotide NMN is used as a starting substrate, adenosine triphosphate, polyphosphate and divalent metal ion salt are added, and the whole-cell enzyme is prepared through a one-pot method. The oxidized nicotinamide adenine dinucleotide phosphate NADP < + > is efficiently and catalytically synthesized by a one-pot method, and the whole-cell catalytic reaction liquid is separated and purified to obtain the NADP < + >. The double-enzyme catalysis method disclosed by the invention is short in reaction route, high in atom economy, capable of reducing the existing production cost, short in production period, easy for downstream separation and purification, and suitable for large-scale and large-scale production of NADP < + >.

The application discloses a device for recycling asphalt in waste asphalt mixture, which comprises a main box body, a bottom plate fixed to the lower side of the main box body, a bottom frame fixedly connected to the lower side of the bottom plate for supporting, a feeding hopper fixedly installed at the top rear side of the main box body, and a cooling and conveying mechanism arranged in the main box body for cooling and conveying the asphalt mixture. The cooling and conveying mechanism is used for uniformly cooling the asphalt mixture to zero below, so that the asphalt material becomes brittle and fragile, and the stone material still maintains the original strength at low temperature due to the material characteristics. The extrusion mechanism uses an elastic rotating structure to apply controllable extrusion force to the mixture, the brittle asphalt material is preferentially crushed, and the stone material is hardly damaged in the extrusion process and can fall into a stone collecting hopper in an intact form. The device greatly improves the recycling rate of the stone material, the intact stone material can be directly used for road base or concrete aggregate, and secondary processing is not needed, so that the cost of stone purchase and reprocessing is remarkably reduced.

The utility model provides an energy-saving acidizing blocking remover preparation and recovery device, which relates to the field of acidizing blocking removers and comprises a preparation unit, a recovery unit is arranged at the bottom of the preparation unit, and an exhaust component is arranged at the top of the preparation unit. The rotating speed of the gear motor can be controlled through the microcontroller, heat is directly transferred to raw materials through the electric heating tube, and the heat preservation sleeve can effectively reduce loss of heat in the reaction tank and reduce energy consumption in the heating process, so that the energy utilization efficiency is improved, the energy consumption is reduced, and the purpose of saving energy is achieved. The recycling unit is arranged at the bottom of the preparation unit, so that the integrated design of preparation and recycling of the acidification blocking remover is realized, the occupied area of equipment and the loss in the material transfer process are reduced, the production efficiency is improved, the used acidification blocking remover can be effectively separated and purified through a nanofiltration membrane and an ultrafiltration membrane, the recycling rate of effective components is improved, and the production cost is reduced. The production cost is reduced.

The present application relates to a primer set, a kit and its application for detecting intraocular bacterial infection, and belongs to the field of biological detection technology. The primer set for detecting intraocular bacterial infection in the present application includes a detection primer pair, a detection probe, an exogenous internal standard primer pair and an exogenous internal standard probe. The present application also discloses a kit for detecting intraocular bacterial infection, including the above-mentioned primer set for detecting intraocular bacterial infection, a PCR reaction premix, a negative quality control product, a positive quality control product, a nucleic acid releaser, a PCR decontamination reagent and an exogenous internal standard. The present application can specifically detect whether the sample contains bacteria by performing qPCR on the eye (intraocular fluid) sample, and can still complete the detection of the sample when the sample amount is small or the bacterial content is low. The minimum detection limit can reach 34CFU / mL, which has the advantages of rapid and simple detection of whether bacteria exist in a single sample, and high sensitivity, strong specificity and a wide detection range.