56results about How to "Reduce purification" patented technology

The invention provides a novel library construction method based on an illumina sequencing platform, and in particular provides a small-fragment DNA library construction method. Aiming at the defects of the small-fragment DNA library construction of the existing illumina sequencing platform, the purification steps can be reduced, the loss and waste of a library are reduced, the cost is lowered, and the working efficiency is improved.

This invention relates to a method of degumming Boehmeria nivea. The process is: fresh Boehmeria nivea (or commercial original Boehmeria nivea) ->immersion in warm water-> mechanical hemp knocking and washing ->boiling off in hyperthermia and heavy pressure (or immersion in warm water) -> mechanical hemp knocking and washing -> refined dry hemp. This method, assuming the characteristics of environmental protection, high efficiency and high qualities, greatly reduces the degumming cost, avoids the pollution of strong acid and strong base in waste solution to the environment, for this process makes use of existing machinery of degumming. Instead of using such chemical materials as strong acid and strong base, the method is high-temp high-pressure cooking, immersion in warm water, mechanical beating and washing for removing extraneous cells of Boehmeria nivea liber.

Process for purifying a recombinant protein including one or a few procedural steps only. The process combines the step of lysis of the host cell, with the purification of the protein of interest, allowing for a rapid and much more efficient process of purification. The conditions used during the purification process are those of a high temperature and a low pH, allowing for thermostable and acid-resistant recombinant proteins to be isolated from a suspension. The invention also relates to purifying recombinant proteins which are fusion proteins, wherein one part of the protein may be selected from an enamel matrix protein, such as amelogenin.

The invention provides a magnetic compatible microsphere for purifying thrombin and a preparation method and application thereof. The magnetic compatible microsphere for purifying the thrombin comprises a magnetic core, a polymer enwrapping material enwrapped outside the magnetic core, and a ligand coupled with the polymer enwrapping material. The magnetic affinity microsphere is characterized in that the ligand can be specifically compatible with the thrombin.

The invention discloses a preparation method of solid phase peptide synthesis atosiban which includes the following steps: taking Rink Amide resins, Rink Amide MBHA resins or Rink Amide AM resins as starting materials and taking Fmoc amino acids as monomers, amino acids are grafted one by one and mercaptopropionic acids (Map(SX)) are protected by the last peptide chain; after protected nonapeptide resins are obtained, the acellular side-chain protective groups and cutting peptides are synchronized; then cutting peptides is carried out, and reduced crude atosiban is collected; the pH value is adjusted to 7.5 to 10.0, and oxidized crude atosiban is collected; target products are obtained by the separation and purification by preparative HPLC(C18 or C8 column). The preparation method is convenient in material source, simplifies technology and reduces cost; and the preparation method is low in the pollution of three wastes and is high in yield, and the preparation method is convenient for being industrialized and has good industrialization prospect.

The invention provides a preparation method of a Salvia miltiorrhiza soft capsule, comprising the following steps of: completely mixing Salvia miltiorrhiza extract with glycerine and polyethylene glycol 400 so that liposoluble constituents such as tanshinone II A are dissolved in a liquid substrate so as to achieve certain solubilization and improve the biological availability of liposoluble constituents of Salvia miltiorrhiza. In the preparation process of the Salvia miltiorrhiza soft capsule, a method for preparing Salvia miltiorrhiza water-soluble component extract is provided, a macroporous resin separation technology is adopted, and ethanol solution is concentrated, atomized and dried after elution; in the obtained extract, the total salvianolic acid compounds account for more than 60% of total solid, wherein the content of salvianolic acid compound danshinolic acid B is between 45% and 60%, and the content of danshensu is controlled between 10% and 20%. The content of danshensu is controlled while the yield of salvianolic acids is improved so that the proportion of the main active ingredient danshinolic acid B is obviously improved. The preparation method is safe and efficient, low-toxic and environmentally-friendly, can satisfy the requirement of industrial production, and has wide and practical application value.

The invention relates to a preparation method of a glutathione-enriched yeast. Culture conditions, feeding strategy and related techniques are optimized to obtain a glutathione-enriched efficient dough yeast product. The method provided by the invention can effectively solve the problems of low glutathione content and poor improvement effect on the dough in the common dried yeast, and the problems of high cost, high oxidation tendency and the like when independently adding glutathione to improve the dough, thereby enhancing the added value of the product.

The present invention discloses a reversible acetylcholinesterase inhibitor huperzine-A synthesis method, wherein the route is defined in the specification. The method of the present invention has advantages of easily-available raw materials, simple operation, high yield, low cost, high purity of the final product, easy quality control and the like, and is suitable for industrial production.

The invention relates to a system and a method of purifying crude caprolactam solution. The system comprises (A) a filtering area, in which resin is utilized to filter out ionic impurities, (B) a detecting unit used for judging whether the solution filtered by the resin conforms to preset detecting standard, (C) a purifying area used for concentrating the solution that is filtered and conforms to preset detecting standard and (D) a first temporary storage device used for receiving and storing the solution that is filtered and is unqualified for preset detecting standard and also used for mixing solution therein with unfiltered crude caprolactam solution and leading the mixture to the filtering area once again. Continuous filtering is available to the system, thereby reducing operation changing frequency of the resin, having no final unqualified caprolactam and prolonging effective operation period of the resin device for filtering out ionic impurity; the system has the advantage of saving material and reducing waste.

The invention relates to a preparation method of a coix seed extract. The method comprises the steps as follows: S1, drying and crushing coix seeds to obtain coix seed flour; S2, removing starch and fat in the coix seed flour through acid treatment and addition of a degreasing agent; S3, performing dynamic superhigh-pressure microfluidization treatment on coix seed pulp to promote dissolution extraction of polysaccharide and protein in the coix seed pulp; S4, adjusting the mass concentration of the coix seed pulp, adding an ionic strength regulator to regulate the ionic strength of the coix seed pulp, and adjusting the pH of the coix seed pulp; S5, adding an organic polymer adsorbent to the coix seed pulp to adsorb protein and polysaccharide agglomerates; S6, performing standing and filtration, collecting a filtered-out organic polymer adsorbent, and sequentially eluting polysaccharide and protein; and S7, removing starch and grease again through low-temperature settlement and centrifugal degreasing to finally prepare a coix seed emulsion containing polysaccharide and protein. The prepared coix seed emulsion is compounded into instant compound coix seed flour.

The invention discloses recombinant escherichia coli for high yield of cytidine monophosphate and application of the recombinant escherichia coli. According to the invention, cytidine kinase gene is obtained by cloning from a cytidine monophosphate production strain; crude enzyme liquid obtained by crushing recombinant bacteria has good catalytic activity and stability; cytidine, adenosine triphosphate (ATP) and Mg < 2 + > are added, the reaction system is simple, the conditions are mild, the period is short, byproducts are fewew, the method is clean and free of pollution, the application is asimple, rapid and efficient production way, and the conversion rate of substrate cytidine and ATP reaches 85% or above.

The invention discloses a complete synthesis method of a natural product paradol, the route being represented as the specification. The method employs feasible raw materials and simple operation, is high in yield and low in cost, is high in final product purity, is controllable in quality, and is suitable for industrial production.

The invention belongs to the technical field of lithium ion battery manufacturing, and discloses a method for purifying lithium difluoroborate and lithium tetrafluoroborate mixed lithium salt and application thereof. According to the preparation method of the high-purity mixed lithium salt with different proportions, industrial-grade lithium oxalate is used as a raw material, the mixed lithium salt with different molar proportions of lithium difluoroborate and lithium tetrafluoroborate is prepared through the processes of synthesis, concentration, auxiliary purification filter membrane purification and the like, and the mixed lithium salt can adapt to various electrolyte formulas, is used for manufacturing lithium ion batteries, and the production cost is reduced; meanwhile, the method provided by the invention avoids the tedious process of recrystallization and the solid-liquid separation operation after crystallization in the prior art, can improve the yield and purity of the product, and simplifies the technological process.

The invention relates to a process for producing ammonium sulfate and hydrogen chloride. The process specifically comprises the following steps: 1, premixing ammonium chloride and sulfuric acid at normal pressure; 2, pumping the obtained sulfuric acid solution of ammonium chloride in step 1 into a tubular reactor, and obtaining ammonium bisulfate and hydrogen chloride gas; and 3, preparing the ammonium bisulfate obtained in step 2 into an aqueous solution, and carrying out an ammonolysis reaction to obtain ammonium sulfate through an ammoniation tower. The ammonium sulfate is prepared by utilizing ammonium chloride, the tubular reactor and the ammoniation tower reactor are mainly selected, a positive pressure reaction is adopted, the reaction conversion rate is improved, the production steps are simplified, the target product yield is improved, and the large-scale continuous production requirements are met; meanwhile, the reaction links are organically combined, the reaction is finished, the generated concentrated sulfuric acid, crystallization mother liquor and the like in the production process are utilized, and the effluent discharge amount is small in the whole production process; and moreover, the ammonium chloride deep-processing problem is effectively solved, and a novel method for producing ammonium sulfate by utilizing high-additional value ammonium chloride is developed.

The invention discloses a local calcium circulation and pure oxygen combustion coupling carbon capture device and process for cement production, and the device comprises a preheater-carbonization furnace-rotary kiln module, a pure oxygen combustion decomposing furnace module and an auxiliary and purification device. The preheater-carbonization furnace-rotary kiln module comprises a raw material feeding device, a first-series cyclone preheater, a carbonization furnace, a second-series cyclone preheater, a smoke chamber, a rotary kiln, a tertiary air pipe, a rotary kiln combustor and a cooling machine. The pure oxygen combustion decomposing furnace module comprises a decomposing furnace, a decomposing furnace combustor, a third-series cyclone preheater and a booster fan. The auxiliary and purification device comprises a first SNCR (Selective Non Catalytic Reduction) device, a second SNCR device, an air distribution device, a first material distribution device, a second material distribution device, a first heat exchange device, a second heat exchange device, a condensation and water removal device, a CO2 collection and storage device, a dust removal device, an SCR (Selective Catalytic Reduction) device and a flue gas exhaust device. Through modular separation and coupling, the CO2 capture and utilization difficulty is reduced, and good economical efficiency is achieved.

The objective of the present invention is to provide a method capable of efficiently removing an impurity from an aqueous solution or a suspension comprising an antibody or an antibody-like molecule and the impurity. The method for purifying an antibody or an antibody-like molecule according to the present invention is characterized in treating an aqueous solution or a suspension comprising the antibody or the antibody-like molecule and an impurity with a water-insoluble inorganic compound, wherein the water-insoluble inorganic compound comprises one or more elements selected from magnesium, calcium and aluminum.

The invention discloses an improved synthetic method of penoxsulam. The improved synthetic method comprises the following steps: taking a strong-alkalinity substance, difluoroethanol and 2-fluoro-6-trifluoromethyl-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyridine-2-yl)benzsulfamide (hereinafter to be referred as tetrafluorosulfamide) as raw materials; taking the strong-alkalinity substance and the difluoroethanol to react to prepare a solution A (in the presence of the difluoroethanol) which has relatively weak alkalinity and contains F2CH1CH2O-; adding the tetrafluorosulfamide to prepare the penoxsulam. Therefore, all the raw materials are added in sequence and the temperature of a reaction system is controlled; side reaction can be effectively reduced; the content of impurities is reduced, the purity of a product is improved or a purification step is reduced.

The invention belongs to the technical field of medicines, and particularly relates to a meropenem intermediate and a preparation method thereof. The meropenem intermediate provided by the invention has a structure as shown in a formula I which is described in the specification. In the formula, R is selected from C1-C6 alkyl groups, optionally substituted phenyl groups or optionally substituted benzyl groups. The preparation method of the meropenem intermediate comprises the following step: mixing an azabicyclo compound, a chloroformate compound, a first organic base and an organic solvent for a reaction to obtain the meropenem intermediate, wherein the azabicyclo compound has a structure as shown in a formula II, the chloroformate compound has a structure as shown in a formula III, and the meropenem intermediate has a structure as shown in the formula I. The meropenem intermediate and the preparation method thereof provided by the invention can effectively overcome the technical defects that in an existing meropenem synthesis process, a phosphorus-containing reagent cannot be recycled, is not friendly to a water body and the ecological environment and pollutes the environment, and solid-liquid waste liquid treatment cost is increased.